Gene NtAOS1 for improving jasmonic acid content in tobacco leaves, cloning method and application of NtAOS1

A cloning method, the technology of jasmonic acid, applied in the field of genetic engineering
CN110305884AActive Publication Date: 2019-10-08YUNNAN ACAD OF TOBACCO AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YUNNAN ACAD OF TOBACCO AGRI SCI
Publication Date
2019-10-08

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Abstract

The invention discloses a gene NtAOS1 for improving jasmonic acid content in tobacco leaves, a cloning method and an application of the NtAOS1. The nucleotide sequence of the NtAOS1 which can improvethe content of jasmonic acid in tobacco is shown in SEQ ID NO. 1; the amino acid sequence encoding the protein is shown in SEQ ID NO. 2; a nucleic acid interference (RNAi) technology is used to designa segment of interference fragment, a gateway technology is used to construct plant expression vector and transform tobacco, and a qPCR technology is used to select NtAOS1 gene transcription level to significantly reduce transgenic plants. Detection of jasmonic acid content shows that the decreased expression level of the gene NtAOS1 results in significantly higher jasmonic acid content than that of wild-type control. The invention provides a material basis for applying the gene NtAOS1 and coded protein thereof to tobacco stress resistance breeding and subsequent research on jasmonic acid synthesis and metabolism pathway in tobacco.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a gene NtAOS1 for increasing the content of jasmonic acid in tobacco leaves, a cloning method and application thereof. Background technique

[0002] Jasmonic acid (JA) and its methyl ester derivative methyljasmonate (MeJA) are important plant growth regulators, which play a regulatory role in the process of plant growth. On the one hand, jasmonic acid can regulate the growth and development of plants, such as the germination and growth of seeds, the growth and development of organs, and the aging and apoptosis of plants. On the other hand, jasmonic acid also participates in the defense response of plants to biotic and abiotic stress. The biosynthetic pathway of JA is also known as the octadecane-like biosynthetic pathway: 13(S)-hydroperoxylinolenic (13-HPOT) is synthesized from linolenic acid catalyzed by lipoxygenase (LOX), 13 -HPOT generates 10,11-dihyd...

Claims

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