Growth-promoting microbial inoculum for inducing systematic resistance of processed tomatoes and application of growth-promoting microbial inoculum

A technology for processing tomatoes and growth-promoting bacteria, which is applied in the direction of application, bacteria, and microorganisms, can solve the problems of unstable application effects of biological control antagonistic bacteria, and achieve the effects of wide application value, green environmental protection cost, and increased germination potential

Active Publication Date: 2021-12-17
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the problems that there are many types of tomato diseases in the existing processing, chemical pesticides are the main control methods, and the application effect of biological control antagonistic bacteria is unstable, and there are no literature and patent reports about the effect of Paenarthrobacter nitroguajacolicus strains in inducing tomato to Botrytis cinerea, early blight and bluegrass. Application of Blight Systemic Resistance

Method used

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  • Growth-promoting microbial inoculum for inducing systematic resistance of processed tomatoes and application of growth-promoting microbial inoculum
  • Growth-promoting microbial inoculum for inducing systematic resistance of processed tomatoes and application of growth-promoting microbial inoculum
  • Growth-promoting microbial inoculum for inducing systematic resistance of processed tomatoes and application of growth-promoting microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation, screening and identification of Paenarthrobacternitroguajacolicus D8 CGMCC No.21584

[0028] (1) Separation and purification

[0029] Collected in 2020 from the rhizosphere soil of beans in the bean field of Xishan Farm in Urumqi, Xinjiang, root surface soil: refers to a small amount of soil that still adheres to the root surface after shaking a large amount of soil attached to the root system. Cut off the root, weigh it (W1), put it in a triangular flask filled with 45mL of sterile normal saline, shake it by hand for 1-2min, wait until the soil adhered to the root surface is washed off, take out the root, and clean it with absorbent paper. After absorbing the water on the root surface, weigh the mass (W2), and W1-W2 is the mass of the root surface soil. Dilute the soil on the root surface to 10-4 times, take 10-1, 10-2, 10-3, 10-4, draw 100 μL of the diluted solution and spread it on the NA medium plate, and place it in a 30°C incubator for culti...

Embodiment 2

[0039] Embodiment 2: the preparation of Paenarthrobacter nitroguajacolicus D8 CGMCC No.21584 microorganism growth-promoting agent

[0040] The present embodiment provides a kind of preparation method of the Paenarthrobacternitroguajacolicus D8 microbial bacterial agent that induces processing tomato system resistance, and concrete steps are as follows:

[0041] (1) Activate the cryopreserved Paenarthrobacter nitroguajacolicus D8 on the plate medium, pick a single colony on the slant medium, and culture it in a 37°C incubator for 24 hours to obtain the activated Paenarthrobacter nitroguajacolicus D8 strain.

[0042] (2) Use a sterile inoculation loop to scrape a ring. Step (1) Inoculate the activated Paenarthrobacter nitroguajacolicus D8 strain into 100mL liquid medium, and shake and cultivate for 24 hours at 37°C with a shaker speed of 180r / min to obtain Paenarthrobacter nitroguajacolicus D8 microbial growth-promoting liquid.

[0043] The aforementioned Paenarthrobacter nitro...

Embodiment 3

[0045] Example 3: Application of Paenarthrobacter nitroguajacolicus D8 CGMCC No.21584

[0046] (1) Effect of Paenarthrobacter nitroguajacolicus D8 strain on germination of processed tomato seeds

[0047] Strain D8 was cultured in a liquid medium at 37°C and 180rpm for 24 hours in shake flasks, and diluted to 10 with normal saline. 4 Multiple, the concentration of bacteria solution is 5.9×10 4 cfu / ml, used for processing tomato seeds for soaking. With water treatment as the blank control, the seeds were soaked in 2% sodium hypochlorite for 10 minutes, rinsed with water for 5 times, then soaked in 75% alcohol for 30 seconds, rinsed with water for 5 times, and the surface was disinfected. The sterilized seeds were soaked in diluted bacterial solution and clear water respectively, kept at 37°C for 4 hours, sowed 10 seeds on a petri dish covered with sterile water-moistened filter paper, each treatment was repeated 3 times, and placed in a 28°C incubator Cultivate in the dark, i...

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Abstract

The invention discloses a growth-promoting bacterium Paenarthrobacter nitroguajalicusD8 which can mediate processed tomatoes to generate induced systematic resistance and has a relatively good disease prevention effect on tomato early blight, gray mold and bacterial wilt, the bacterium is screened in green bean plant rhizosphere soil in a green bean field in a farm in West Mountain of Wujiang Mountain in Xinjiang, the bacterium is known to be a novel bacterium similar to Paenarthrobacter nitroguajalicus through classification and systematic identification and analysis, a Paenarthrobacter nitroguajalicus D8 growth-promoting microbial inoculum is prepared by using a liquid fermentation technology, tomato seeds are treated and processed, such that the bud length, the germination rate and the germination potential of the tomato seeds are significantly increased, and the tomato root length, the fresh weight and the plant height are significantly improved. The bacterial liquid of the strain D8 can obviously increase the jasmonic acid content of the processed tomatoes and maintain the jasmonic acid content for a certain time, so that the processed tomatoes generate systematic resistance, and the disease resistance of the processed tomatoes to three pathogenic bacteria is enhanced. The novel growth-promoting microbial inoculum provided by the invention is used for processing tomato epidemic situation prevention and control, is green, environment-friendly and low in cost, and has wide application value for expanding the technical field of application of microbial growth-promoting bacteria strains.

Description

technical field [0001] The present invention relates to the technical field of microbial bacterium agent and application thereof, be specifically related to a kind of Paenarthrobacter nitroguajacolicus D8 microbial growth-promoting bacterium agent and its application technical field of inducing processed tomato to produce systemic resistance. Background technique [0002] Tomato processing is a dominant industry in Xinjiang's characteristic economy, one of the three major industries in Xinjiang, accounting for 18% of the world's total output, and has an important position in China and the world. Early blight, bacterial wilt and botrytis cinerea are three serious diseases that occur during the growth of processed tomatoes, which cause a 10%-30% reduction in tomato output every year. In recent years, with the increase of the planting area of ​​processed tomatoes, the occurrence of diseases has become more and more serious. However, the situation of regional layout and industri...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G13/00A01G22/05C12R1/01
CPCC12N1/20A01G13/00A01G22/05Y02A40/10
Inventor 林青娄恺史应武霍向东杨红梅楚敏高雁曾军包慧芳王宁
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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