Method for detecting tanshinol and its metabolites in biological sample

A technology of biological samples and detection methods, applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2018-11-13
北京鸿测科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing detection methods of danshensu metabolites can detect very few danshensu metabol

Method used

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  • Method for detecting tanshinol and its metabolites in biological sample
  • Method for detecting tanshinol and its metabolites in biological sample
  • Method for detecting tanshinol and its metabolites in biological sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] 1 Experimental instruments and materials

[0057] DIONEX Ultimate 3000 ultra-high performance liquid chromatography analysis system: American ThermoFisher Company;

[0058] LTQ-Orbitrap XL mass spectrometer: American Thermo Fisher Company, equipped with electrospray ion source (ESI) and Xcalibur 2.1 workstation;

[0059] Grace Pure TM SPEC 18 -Low SPE cartridge (500mg / 3mL);

[0060] Milli-Q Synthesis ultrapure water purification system: Millipore, USA;

[0061] R200D electronic analytical balance (1 / 100,000): German Sartorius company;

[0062] KQ-250DE CNC ultrasonic cleaner: Kunshan Ultrasonic Instrument Co., Ltd.

[0063] Danshensu reference substance: product of Chengdu Master Biological Technology Co., Ltd. (purity greater than 98%);

[0064] Formic acid (chromatographically pure): Germany Merck company;

[0065] Methanol (mass spec pure) and acetonitrile (mass spec pure): American Thermo Fisher Company.

[0066] Sprague Dawley (SD) rats (male, weighing 220...

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Abstract

The invention discloses a method for detecting tanshinol and its metabolites in a biological sample. The method is characterized by comprising the following steps that the biological sample is treated; a solution to be detected is detected by adopting an ultra-high performance liquid chromatography-mass spectrometer. By adopting the detecting method, more tanshinol metabolites in the biological sample can be detected, and accordingly a foundation is laid for illumination of tanshinol metabolism pathways.

Description

technical field [0001] The invention relates to a detection method for danshensu and its metabolites in biological samples. Background technique [0002] Salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza Bge., a plant in the Labiatae family. It is a traditional medicine for promoting blood circulation and removing stasis in my country. Modern research shows that the main chemical components of Danshen are fat-soluble diterpene quinones and water-soluble phenolic acids. Danshensu, as an index component of Danshen, has various pharmacological effects such as anti-inflammation, inhibition of thrombosis, neuroprotection, anti-tumor, improvement of heart function, and expansion of coronary arteries. It is often used as a quality control index of Danshen medicinal materials or Danshen compound preparations. However, the in vivo metabolic process and metabolites of danshensu are still unclear, and further research is urgently needed. [0003] "Study on the Ph...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/06
CPCG01N30/02G01N30/06G01N30/72G01N2030/062
Inventor 王志斌张加余张连中
Owner 北京鸿测科技发展有限公司
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