75 results about "Schizosaccharomyces pombe" patented technology

The present invention is directed to telomerase nucleic acids and amino acids. In particular, the present invention is directed to nucleic acid and amino acid sequences encoding various telomerase protein subunits and motifs, including the 123 kDa and 43 kDa telomerase protein subunits of Euplotes aediculatus, and related sequences from Schizosaccharomyces, Saccharomyces sequences, and human telomerase. The present invention is also directed to polypeptides comprising these telomerase protein subunits, as well as functional polypeptides and ribonucleoproteins that contain these subunits.

The present invention is directed to novel telomerase nucleic acids and amino acids. In particular, the present invention is directed to nucleic acid and amino acid sequences encoding various telomerase protein subunits and motifs, including the 123 kDa and 43 kDa telomerase protein subunits of Euplotes aediculatus, and related sequences from Schizosaccharomyces, Saccharomyces sequences, and human telomerase. The present invention is also directed to polypeptides comprising these telomerase protein subunits, as well as functional polypeptides and ribonucleoproteins that contain these subunits.

Screening and application of yeast with high ethanol yield and low fusel oil yield in Chinese Maotai-flavor liquor production belong to the technical field of biological engineering. The strain is any one strain selected from pichia anomala CGMCC4740, schizosaccharomyces pombe CGMCC4744, zygosaccharomyces bailii CGMCC4745, trichosporon asahii CGMCC4746, saccharomyces cerevisiae CGMCC4747, kazachstania exigua CGMCC4748, pichia fermentans CGMCC4750. The strain is separated from various Daqu, fermented grains, brewing raw materials and brewing environment of several Chinese famous distilleries; the screening method comprises the following steps: diluting distiller yeast samples, fermented grains, brewing raw materials or brewing environment substances, coating on a TTC primary screening plate, selecting a colony with an obvious red color, inoculating the colony in a shake flask for culture, performing secondary screening, determining the contents of ethanol and fusel oil of the fermentation broth by a distillation specific gravity method and HS-SPME-GC-MS respectively, screening the strain with high ethanol concentration and low fusel oil concentration. The strain is applicable to food industry and brewed wine industry.

The present invention relates to a transformant, containing a lactate dehydrogenase gene which is introduced into Schizosaccharomyces pombe as a host, in which a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated.

The invention discloses a strain produced by screening beta-damascenone in Chinese liquor brewing by utilizing flavor directional technology and application thereof, belonging to the fields of biology engineering technology and brewing biotechnology. The beta-damascenone is an important trace characteristic flavor substance in Chinese Fen-flavor liquor. The strain is any one of Pichia anomala CGMCC4740, Issatchenkia orientalis CGMCC4741, Saccharomycopsis fibuligera CGMCC4742, Pichia farinosa CGMCC4743, Schizosaccharomyces pombe CGMCC4744, Trichosporon asahii CGMCC4746, Saccharomyces cerevisiae CGMCC4747, Kazachstania exigua CGMCC4748, Hanseniaspora osmophila CGMCC4749, Pichia fermentans CGMCC4750, Pichia membranifaciens CGMCC4751 and Clavispora lusitaniae CGMCC4752. The strain is obtainedby separating Daqu, Xiaoqu, fermented grains and brewing materials in the brewing environment of many famous wineries in China. The strain can be applied to the field of traditional food brewing and preparation of beta-damascenone biological spices.

The invention relates to a composite microbial agent for treating domestic garbage and a method for treating the domestic garbage by using the composite microbial agent. The composite microbial agent for treating the domestic garbage is prepared from the following components: brevendimonas diminuta, pseudomonas stutzeri, pseudomonas maltophilia, pseudomonas fluorescens, pseudomonas putida, candida utilis, candida lipolytica, schizosaccharomyces pombe, schizosaccharomyces octosporus, bacillus megatherium, bacillus subtilis, bacillus cereus, alcaligenes faecalis, clostridium beijerinckii, achromobacter denitrificans, nocardia coralline, and the like, wherein the parts by weight of the brevendimonas diminuta are 1-5, the parts by weight of the pseudomonas stutzeri are 1-5, the parts by weight of the pseudomonas maltophilia are 1-5, the parts by weight of the pseudomonas fluorescens are 1-5, the parts by weight of the pseudomonas putida are 1-5, the parts by weight of the candida utilis are 1-5, and the parts by weight of the candida lipolytica are 1-5.

The invention relates to the technical field of liquor production, particularly to a multi-microbe solid fermentation ethanol and acetic acid production key microbe quantitative analysis method. The method comprises performing quantitative PCR (polymerase chain reaction) analysis on HIS3 genes of saccharomyces cerevisiae, AHD1 genes of schizosaccharomyces pombe and 16S rRNA (ribonucleic acid) fragments of lactobacillus to analyze the change tendency of the saccharomyces cerevisiae, the schizosaccharomyces pombe and the lactobacillus in liquor yeasts and fermented grains and accordingly to predict the yield of ethanol. The multi-microbe solid fermentation ethanol and acetic acid production key microbe quantitative analysis method has the advantages of being high in detecting speed and sensitivity, good in accuracy and specificity and free from cultivation.

This invention relates to a class of checkpoint genes and their polypeptide products which control progression through the cell cycle in eukaryotic cells. In particular, this invention relates to Schizosaccharomyces pombe rad3 gene, to its human homologue (ATR), and to their encoded proteins. The invention further relates to assay methods for selecting compounds which modulate the activity of the polypeptide products of these checkpoint genes and the use of the selected compounds in anticancer therapy.

The invention discloses a method for effectively improving L-malic acid production intensity of Aspergillus oryzae pp25, belonging to the field of genetic engineering. According to the invention, Aspergillus oryzae pp25 is used as a starting strain, a C4-dicarboxylate transport protein gene C4T318 of the Aspergillus oryzae pp25 and a malic acid transport protein gene maeI of Schizosaccharomyces pombe are expressed to obviously reduce the intracellular accumulation of malic acid, and finally, the shake flask yield of the L-malic acid is up to 93.17+ / -2.10g / L and is increased by 47.4%; the concentration of succinic acid is 5.17+ / -0.49g / L and is reduced by 41.8%; and the conversion rate of the malic acid relative to glucose is 0.85g / g and is increased by 49.1%. Meanwhile, the fermentation period is shortened from 90 hours to 80 hours, and the production intensity is up to 1.16g / L.h and is increased by 65.7%.

The invention discloses a method for producing edible alcohol by fermenting thick mash of defective red dates at a high temperature. According to the advantages of high sugar content of defective red dates, defective red dates are taken as raw materials, by virtue of high-temperature alcohol-resistant Schizosaccharomyces pombe, and the thick mash of red dates is subjected to high-temperature alcoholic fermentation to obtain red dates edible alcohol dates. According to the method, the conversion rate from carbohydrates in red dates to ethanol reaches above 80%, and the produced red dates edible alcohol is in line with hygienic standards of national edible alcohol.

The invention relates to the technical field of microbial gene engineering, in particular to brand-new ketopantolactone reductase derived from schizosaccharomyces pombe and application of the brand-new ketopantolactone reductase. The ketopantoate reductase has excellent enzymatic activity to ketopantolactone and can catalyze asymmetric reduction of the ketopantolactone to generate D-pantolactone.A one-bacterium double-enzyme reaction system is further constructed, the ketopantolactone reductase generated by double-enzyme recombinant cell induction is coupled with glucose dehydrogenase, asymmetric reduction of a substrate, namely the ketopantolactone, is catalyzed to generate the D-pantolactone, beta-nicotinamide-adenine dinucleotide phosphate (NADP<+>) is reduced into nicotinamide adeninedinucleotide phosphate (NADPH) through dehydrogenation of a cosubstrate, namely glucose, and thus coenzyme circulation is realized. The catalytic efficiency of the glucose dehydrogenase is much higher than that of the ketopantolactone reductase, thus reduction reaction can be promoted to be conducted at the high speed, the reaction efficiency is greatly improved, and the cost is lowered.

The invention discloses a method for producing tea wine by utilizing eurotium cristatus. The method comprises steps as follows: a tea solid medium is prepared; eurotium cristatus FBKL3.0002 is activated on a PDA (potato dextrose agar) solid medium and enriched and cultured at the temperature of 30 DEG C in a PDA liquid medium for 5-7 days; 15% by mass of the eurotium cristatus FBKL3.0002 is inoculated to the tea solid medium and fermented at 30 DEG C for 10-15 days; the tea solid medium is prepared; schizosaccharomyces pombe CICC1360 is activated on a wort solid medium, cultured for 2-3 d in a wort slant solid medium, inoculated to a wort liquid medium and cultured at the temperature of 28 DEG C for 2 d; 8%-10% by volume of schizosaccharomyces pombe CICC1360 is inoculated and fermented at the temperature of 28 DEG C for 5-7 d; ethanol is steamed out, the alcohol content is controlled to be about 40 v / v, and the tea wine is obtained. With the adoption of the method for producing the tea wine by utilizing eurotium cristatus, the utilization rate of effective ingredients of tea can be increased, and the quality of the tea wine is better.

The invention discloses an engineering bacteria of fission yeast schizosaccharomyces pombe which has cellulase activity and a construction method thereof. A constructed expression carrier of the fission yeast schizosaccharomyces pombe which contains cellulase genes is led into the yeast schizosaccharomyces pombe and the fission yeast schizosaccharomyces pombe provided by the invention is obtained. The construction method of engineering bacteria of fission yeast schizosaccharomyces pombe is that the fission yeast schizosaccharomyces pombe which contains cellulase genes is constructed and then led into the fission yeast schizosaccharomyces pombe to gain a recombinant yeast transformant, and then the recombinant yeast cultured to lead cellulase genes to express the carrier. The invention makes up the shortcomings of the traditional preparation method of cellulase, a prokaryotic expression system, and the expression system of saccharomyces cerevisiae and pichia. The invention is applied to the production of industrial cellulase and has advantages of high activity of cellulase, short growth cycle, and low cost of extraction.

A traditional Chinese medicinal preparation for treating damp turbidity and spleen stagnation type hyperlipidemia is prepared by using malt, millet sprout, rice grain sprout, Gynostemma pentaphylla, lotus leaf, corn silk, Chinese yam, dried orange peel, cassia seed, hawthorn fruit, Mythic Fungus, wolfberry fruit, Poria cocos, white hyacinth bean, lotus seed, black soybean, jujube, Coriolus versicolor(L.ex Fr.)Quel., dahurian angelica root, coix seed, Ligustri Lucidi Fructus and Semen Phaseoli. A preparation method of the traditional Chinese medicinal preparation comprises the following steps: decocting the above raw materials with water, cooling the obtained filtrate to room temperature, and inoculating Saccharomyces cerevisiae or Sac.carlsbergensis Hansen or Schizosaccharomyces pombe or Nematspora gossypii, wherein the amount of the above inoculated bacterium accounts for 1-5% of the volume of the water extract filtrate; fermenting at 20-32DEG C for 30-48h to obtain a suspension fermentation liquid, concentrating to obtain an extract paste, and drying at 80-100DEG C; and grinding the dried extract paste to fine powder having a particle size of 150mum or less to obtain an inactivated thallium and metabolite fine powder, detecting the protein content reaching 40% or more, and filling to a capsule. The clinic cure rate, the significant efficiency, the effective rate and the total effective rate of the preparation are 32.90%, 23.81%, 32.14% and 92.86% respectively.

The invention discloses an environmental-friendly restoring agent for heavy metals in soil. The environmental-friendly restoring agent is prepared from the following materials by weight: 15-28 parts of carboxymethyl chitosan, 17-31 parts of polyacrylamide,7-19 parts of polyaluminum chloride, 20-39 parts of polyaluminum ferric chloride, 14-31 parts of bentonite, 17-28 parts of sepiolite, 7-11 partsof calcium magnesium phosphate fertilizer, 28-33 parts of calcium superphosphate, 20-31 parts of magnesium sulfate, 15-27 parts of sodium silicate, 13-23 parts of boric acid, 21-31 parts of borax, 28-37 parts of zinc sulfate, 11-21 parts of amino acids, 9-14 parts of humic acid, 13-18 parts of a compound enzyme preparation, 4-6 parts of slash pine needles, 4-6 parts of Pseudomonas fluorescens powder, 7-11 parts of halotolerant yeast powder and 10-15 parts of Schizosaccharomyces pombe powder. The environmental-friendly restoring agent for heavy metals in soil can supplement a large amount of organic matters and nitrogen-phosphorus-potassium nutrient sources, reduce the activity of heavy metals in soil, improve the physical and chemical properties of soil, lower soil acidity, and achieve the purpose of restoring soil activity and fertility.

The invention provides a preparation and quick screening method of yeast fusant. In order to realize mass production of coenzyme Q10, a method of protoplast fusion is utilized to fuse schizosaccharomyces pombe and hensenula polymorpha and combined with a method for efficiently screening target yeast fusant to quickly and efficiently obtain target yeast fusant having excellent characteristics of two yeast strains, and the target yeast fusant has the advantages of high growing speed, resistance to high temperature and capability of efficiently producing the coenzyme Q10. According to the yeast fusant screening method, growing performance including growing speed, resistance to high temperature and target metabolite of thalli is taken as a screening marker; specifically, temperature tolerance and a culture medium containing vitamin K3 which is a structural analog of the coenzyme Q10 are taken as screening strategies, and the target fusant can be directly screened in a high-throughput manner. The preparation and quick screening method is a yeast improving method which is simple, quick, efficient, economical, practical and safe.

The invention belongs to the field of biological feed, and relates to a preparation method of recombinant manganese peroxidase and application of the recombinant manganese peroxidase in degradation ofChinese herbal medicine lignin. The method comprises the following steps: firstly, carrying out preference optimization on a gene sequence of manganese peroxidase coming from Irpex lacteus, carryingout PCR amplification by taking the optimized gene as a template, connecting the optimized gene with pREP, then converting the connected gene into escherichia coli competent cells to obtain recombinant shuttle plasmids, and introducing the recombinant shuttle plasmids into defective schizosaccharomyces pombe competent cells to obtain recombinant manganese peroxidase engineering bacteria; and performing culture to obtain a crude enzyme liquid, mixing the crude enzyme liquid with Chinese herbal medicines, and then adding calcium chloride, manganese sulfate, laccase, glucose oxidase and a small molecular substance activator for a multi-enzyme synergistic enzymolysis reaction. According to the method provided by the invention, a large amount of manganese peroxidase can be rapidly obtained, industrial production of the manganese peroxidase is realized, a good and stable peroxidase product is prepared, and effective degradation of Chinese herbal medicine lignin is realized.

The invention discloses a method for preparing coenzyme Q10. The method of the invention comprises the following steps: switching-in schizosaccharomyces pombe to culture medium containing solanesol, carrying out fermentation in the supercritical CO2 environment with temperature ranging from 25 DEG C to 35 DEG C and pressure ranging from 5MPa to 20 MPa, thus obtaining the coenzyme Q10.The method of the invention makes full use of dissolution characteristics of the supercritical CO2 to increase solubility of the solanesol, thus effectively reducing product inhibiting effect in the process of conversion, providing a high anaerobic environment for yeast, changing respiratory chain oxygen supply mode of microorganism, and promoting synthesis of the coenzyme Q10; in addition, in the method of the invention, the solanesol is added as substrate of the coenzyme Q10, thus improving speed and output of the synthesis of the coenzyme Q10.Experiments indicate that the output of the coenzyme Q10 prepared by the method of the invention is increased by 60-162% compared with the output thereof prepared by ordinary fermentation methods.

The invention discloses a method for heterologous expression and purification of members of glucose transporter family including CLUT1, CLUT2, and CLUT3 on human cell membrane, which comprises the following steps of: constructing an inducible fusion protein expression vectors of GST-CLUT1, GST-GLUT2, and GST-GLUT3 and transforming fission yeast; identifying transformants of the fission yeast; induced expression of the three fusion proteins of GST-CLUT1, GST-GLUT2, and GST-GLUT3 in the fission yeast; identifying the expression of the genes of GLUT1, GLUT2 and GLUT3 in the fission yeast; separating and purifying the three fusion protein; detecting and identifying the three separated and purified fusion proteins; separating and purifying human proteins of GLUT1, GLUT2 and GLUT3 from the three fusion proteins respectively.

The invention provides an engineering bacteria of fission yeast schizosaccharomyces pombe which has a function of defensin and a construction method thereof. A constructed expression carrier of the fission yeast schizosaccharomyces pombe which contains defensin genes is led into the yeast schizosaccharomyces pombe and the fission yeast schizosaccharomyces pombe provided by the invention is obtained. The defensin genes are inserted in the polyclonal locus of the expression carrier of the fission yeast schizosaccharomyces pombe and the constructed expression carrier of the fission yeast schizosaccharomyces pombe is led into the fission yeast schizosaccharomyces pombe to gain recombinant fission yeast schizosaccharomyces pombe which is cultured to get the expression of defensin genes. The invention makes up the shortcomings of the traditional preparation method of defensin and the expression system of pronucleus, saccharomyces cerevisiae and pichia. The invention is applied to the production of industrial defensin and has advantages of large production scale, high activity of defensin, strong sterilizing ability, short growth cycle, and low cost of extraction.

Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in which Schizosaccharomyces pombe is used as a host, a lactate dehydrogenase gene of Lactobacillus pentosus is introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.

Provided are methods and compositions for reducing damage to skin by ultraviolet light and other agents that cause distortion to double stranded DNA and methods for reducing damage to other organs due to such DNA distortion. These compositions comprise a novel truncated UV damage endonuclease (a truncated derivative of Uvde 1 (UVDE, Uvelp) of Schizosaccharomyces pombe) in conjunction with a cell penetrating peptide, together with components suitable for topical application or other administration to a human or animal in need of treatment to reduce damage to due distortion of double-stranded DNA. Methods for reducing DNA distortion-induced damage or deterioration of condition comprise the step of administering a composition comprising the truncated Uvde 1 of the present invention in conjunction with a fused cell penetration peptide or with a noncovalently bound cell penetration peptide to the skin or other organ, or by other route of administration. Compositions for topical application are also useful for cosmetic or cosmeceutical use.

Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in which Schizosaccharomyces pombe is used as a host, a lactate dehydrogenase gene of Lactobacillus pentosus is introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.

Compounds are identified simultaneously as having antibiotic activity targeting a specific microbial protein and having no or limited toxicity against eukaryotic cells by expressing the microbial protein in eukaryotic cells by expressing the microbial protein in eukaryotic cells which then are used to screen candidate antibiotic compounds. Preferably, yeast cells such as Schizosaccharomyces pombe are transfected with and express a target bacterial protein such as FtsZ or MreB, optionally as a fusion with a reporter protein, and these transfected cells are used to screen libraries of compounds simultaneously for activity against the bacterial protein and lack of toxicity against the yeast cell.

The present disclosure describes DNA damage endonucleases which exhibit broad specificity with respect to the types of structural aberrations in double stranded DNA. These enzymes recognize double stranded DNA with distortions in structure, wherein the distortions result from photoproducts, alkylation, intercalation, abasic sites, mismatched base pairs, insertion deletion loops, cisplatin adducts and other types of base damage (for example, uracil resulting from cytosine deamination). The UVDE (Uve1p) of Schizosaccharomyces pombe, certain truncated forms of that UVDE (lacking from about 100 to about 250 amino acids of N-terminal sequence) and certain endonucleases from Homo sapiens, Neurospora crassa, Bacillus subtilis, Bacillus anthracis, Methanococcus jannaschii, and Deinococcus radiodurans. The present disclosure further provides methods for cleaving double stranded DNA having structural distortions as set forth herein using the exemplified endonucleases or their stable, functional truncated derivatives.

A fission yeast having non-functional dga1 and plh1 genes, which is incapable of synthesizing triacylglycerols, is disclosed. The fission yeast is susceptible to lipotoxicity including lipoapoptosis, and is useful for screening of compounds that inhibit or prevent lipotoxicity or lipoapoptosis. The fission yeast strain may be transformed to express a mammalian enzyme involved in triacylglycerol synthesis, and therefore is also useful for screening of compounds that inhibit or prevent triacylglycerol synthesis, which activity is conferred by the mammalian enzyme.

The invention discloses primers and a method for rapidly identifying and quantifying schizosaccharomyces pombe, and belongs to the technical field of bioengineering. A specific primer pair R1A1F / R1A1R is designed for schizosaccharomyces pombe ethanol dehydrogenase, the sequences are shown as SEQ ID NO:2 and SEQ ID NO:3, and rapid qualitative and quantitative detection of schizosaccharomyces pombe in samples such as yeast for making hard liquor and fermented grains can be achieved through a PCR technology; and accuracy is high, effectiveness is good, and sensitivity is high. The technical scheme of the invention is suitable for fermented food finished products or samples taken from the fermentation process of fermented food.