3results about How to "Maintain native conformation" patented technology

The application discloses a kind of for sweat glucose concentration monitoring method, it is related to sweat monitoring field, comprising the following steps: step 1, enzyme-fixed chain conjugate synthesis: glucose dehydrogenase is reacted with bilirubin oxidase respectively with bifunctional crosslinking agent and is purified, obtain enzyme-crosslinking agent intermediate;It is clicked with azide modified DNA fixed chain chemistry reaction again, and after purification, obtain GDH-fixed chain and BOx-fixed chain conjugate;Step 2, preparation of circular DNA template: after annealing hybridization of phosphorylated template chain and primer chain, it is connected into ring by T4 DNA ligase;Step 3, rolling circle amplification self-assembly package enzyme DNA hydrogel;The present application uses super-flexible DNA hydrogel as flexible electrode material, realizes the efficient fixation and continuous stable catalysis of GDH and BOx, and constructs flexible sweat-based EBFC self-powered sensor as flexible bioelectrode, to realize continuous non-invasive blood glucose monitoring.

The invention relates to a method for purifying influenza viruses cultured by chick embryos, and belongs to the technical field of biological medicines. The method comprises the following steps: specifically capturing inactivated virus liquid from a chick embryo through microspheres; the microspheres swell and adsorb viruses at low temperature, the network structure of the microspheres is triggered to shrink through temperature control, complete virus particles are released, and damage caused by chemical competitive elution is avoided; and after the released virus is split, refining and purifying through sucrose density gradient centrifugation and molecular sieve chromatography. Through the synergistic effect of impurity removal, specific capture and mild release, ovalbumin residues are reduced, virus hemagglutinin is kept, and an industrialized feasible path is provided for preparing influenza vaccine antigens with high purity and high immunogenicity.

The application provides a method for preparing apocynum venetum polysaccharide with a triple helix structure through fermentation of bacillus velezensis and application thereof. The apocynum venetum is subjected to fermentation treatment by bacillus velezensis, and through synergistic effect of various extracellular enzymes secreted by the strain, efficient degradation of the cell wall of the apocynum venetum and sufficient release of the polysaccharide are realized. Experiments show that the polysaccharide obtained by the application has a significant triple helix structure feature, and the polysaccharide obtained by ultrasonic cellulase complex method and cellulase method does not have the structure. It is found for the first time that fermentation of bacillus velezensis can induce apocynum venetum polysaccharide to form a stable triple helix structure, and directional construction of the higher structure of the apocynum venetum polysaccharide is realized. The apocynum venetum polysaccharide with the triple helix structure can be used as a probiotic preparation for promoting generation of short-chain fatty acids in the intestinal tract and improving intestinal microecology, and can be applied to the fields of functional food and intestinal health.