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Lipase/polyion liquid-styrene microsphere/hydrogel catalytic material as well as preparation method and application thereof

A technology of styrene microspheres and polyionic liquids, applied in the direction of microsphere preparation, biochemical equipment and methods, microcapsule preparations, etc., can solve problems such as weak force, destruction of enzyme conformation, difficult substrate contact, etc., to prevent Falling off, not easy to fall off, easy to separate effect

Active Publication Date: 2020-06-16
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the articles reported in recent years, most of the enzymes are adsorbed on anion-cation resins, mesoporous materials, and the surface of polystyrene microspheres. limited by disadvantages

Method used

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  • Lipase/polyion liquid-styrene microsphere/hydrogel catalytic material as well as preparation method and application thereof
  • Lipase/polyion liquid-styrene microsphere/hydrogel catalytic material as well as preparation method and application thereof
  • Lipase/polyion liquid-styrene microsphere/hydrogel catalytic material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] (1) Weigh 2.4g of PVA (polyvinyl alcohol) and 2.4g of sodium chloride into 80mL of pure water, and mechanically stir at 95°C until dissolved to obtain the water phase;

[0054] (2) Styrene (5.208g), divinylbenzene (3.256g), toluene (4.608g), and initiator azobisisobutyronitrile (0.2g, 3.0wt%) were mixed and purged with nitrogen for 20min , remove the oxygen in the solution to obtain the mixed solution as the organic phase;

[0055] (3) The organic phase was added to the water phase, and the reactor was vigorously stirred for 1 h to prepare an oil-in-water suspension, and suspension polymerization was carried out at 70° C. for 6 h, and the mechanical stirring rate was kept constant at 650 rpm;

[0056] (4) After the polymerization reaction is finished, first use ethanol, then thoroughly wash with hot water, remove unreacted monomers, and obtain polystyrene microspheres;

[0057] (5) Weigh 0.5g polystyrene microspheres in 5mL lipase fermentation broth, then put them on a...

Embodiment 2

[0060] (1) Weigh 2.4g of PVA (polyvinyl alcohol) and 2.4g of sodium chloride into 80mL of pure water, and mechanically stir at 95°C until dissolved to obtain the water phase;

[0061] (2) styrene (5.208g), divinylbenzene (3.256g), toluene (4.608g), 1-vinyl-3-butylimidazolium bromide (1.450g) and initiator azobisisobutyl Nitrile (0.436g, 3.0wt%) mixed, and purged with nitrogen for 20min, removed the oxygen in the solution, and obtained the mixed solution as the organic phase;

[0062] (3) The organic phase was added to the water phase, and the reactor was vigorously stirred for 1 h to prepare an oil-in-water suspension, and suspension polymerization was carried out at 70° C. for 6 h, and the mechanical stirring rate was kept constant at 650 rpm;

[0063] (4) After the polymerization reaction finishes, first use ethanol, then thoroughly wash with hot water, to remove unreacted monomers, obtain polyionic liquid-styrene microspheres;

[0064] (5) Weigh 0.5g polyionic liquid-styre...

Embodiment 3

[0067] (1) Weigh 2.4g of PVA (polyvinyl alcohol) and 2.4g of sodium chloride into 80mL of pure water, and mechanically stir at 95°C until dissolved to obtain the water phase;

[0068] (2) styrene (5.208g), divinylbenzene (3.256g), toluene (4.608g), 1-vinyl-3-butyl imidazolium bromide (2.890g) and initiator azobisisobutyl Nitrile (0.479g, 3.0wt%) was mixed, and purged with nitrogen for 20min, removed the oxygen in the solution, and obtained the mixed solution as the organic phase;

[0069] (3) The organic phase was added to the water phase, and the reactor was vigorously stirred for 1 h to prepare an oil-in-water suspension, and suspension polymerization was carried out at 70° C. for 6 h, and the mechanical stirring rate was kept constant at 650 rpm;

[0070] (4) After the polymerization reaction finishes, first use ethanol, then thoroughly wash with hot water, to remove unreacted monomers, obtain polyionic liquid-styrene microspheres;

[0071] (5) Weigh 0.5g polyionic liquid-...

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Abstract

The invention relates to a lipase / polyion liquid-styrene microsphere / hydrogel catalytic material as well as a preparation method and application thereof, and belongs to the field of enzyme immobilization. The preparation method comprises the steps: performing polymerization reaction on styrene and imidazolium bromide to form polyion liquid-styrene microspheres; dispersing the polyion liquid-styrene microspheres into a lipase solution, immobilizing lipase onto the polyion liquid-styrene microspheres through physical adsorption to obtain lipase / polyion liquid-styrene microspheres, and carrying out secondary immobilization on the lipase / polyion liquid-styrene microspheres in hydrogel. The obtained catalytic material is environment-friendly and non-toxic, is easy to separate from a reaction system, and can be recycled, so that the catalytic material has a wide application value in various fields such as biocatalysis and food industry.

Description

technical field [0001] The invention relates to a lipase / polyionic liquid-styrene microsphere / hydrogel catalytic material and its preparation method and application, belonging to the field of enzyme immobilization. Background technique [0002] Lipase is a class of enzymes that can catalyze reactions such as hydrolysis, esterification, transesterification, and polypeptide synthesis. Lipase widely exists in various tissues of animals and plants and many microorganisms, such as bacteria, yeast and mold, which can secrete lipase. Enzyme catalysis is one of the green production technologies. It has the advantages of mild conditions, high catalytic efficiency, good specificity, environmental friendliness, high product safety, and low energy consumption. Environmental advantages. Enzyme catalysis has become a new development trend due to the continuously growing demand for environmental protection and the implementation of national energy-saving emission reduction and low-carbon...

Claims

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Application Information

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IPC IPC(8): C08F212/08C08F212/36C08F226/06C08F2/18B01J13/14C12N11/082
CPCC08F212/08C08F2/18B01J13/14C12N11/08C12N9/20C12Y301/01003C08F212/36C08F226/06
Inventor 田晶冯暖费旭李尧徐龙权王一
Owner DALIAN POLYTECHNIC UNIVERSITY
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