Method for recovery of DNA by using magnetic nanocomposite and reagent kit therefor

A composite material and magnetic nanotechnology, applied in the biological field, achieves the effect of simple method, good biocompatibility and prolonging service life

Inactive Publication Date: 2007-05-30
JILIN UNIV
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Currently, there is no technology for

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Example 1

[0045] Preparation of magnetic nanocomposites

[0046] Weigh 54.90g of FeCl 3 ·6H 2 O and 20.20g FeCl 2 ·4H 2 O, dissolve with 200ml of water that has been purged with nitrogen and deoxygenated to obtain a mixed solution. Take 800ml of water in a 1L round-bottomed flask with nitrogen and remove oxygen, add 50ml of concentrated ammonia water with a concentration of 25-28% by mass, and quickly pour the above-mentioned iron salt mixed solution into it under vigorous stirring, at 80℃ Reaction for 1h. After the reaction is completed, a 0.1T permanent magnet is used to separate the black solid from the reaction solution, and the obtained solid is washed 3 to 5 times with high purity water to obtain Fe with a particle size of 6 to 10 nm. 3 O 4 Nanoparticles.

[0047] Weigh out 5.0g Na 2 SiO 3 (SiO 2 The content is 45%) dissolved in 1000ml of water, the pH of the solution is adjusted to 9.6 with cationic resin, and 2g of the above-mentioned synthetic Fe is added to it. ...

Example Embodiment

[0049] Example 2

[0050] DNA recovery kit

[0051] Prepare a DNA recovery kit using magnetic composite materials, which includes:

[0052] 0.5ml of the magnetic nanocomposite material solution prepared in Example 1 with a concentration of 20mg / ml

[0053] 100ml dissolving solution, its components are 6mol / L NaI solution, 0.5ml Tris-HCl, pH=6.4, 25mmol / l EDTA;

[0054] 100ml washing liquid II, its component is 1.5mol / l sodium acetate;

[0055] 10ml eluent is deionized water.

Example Embodiment

[0056] Example 3

[0057] DNA recovery kit

[0058] Prepare a DNA recovery kit using a magnetic composite material. The magnetic nanocomposite material solution and washing solution II are the same as those in Example 2, and further include:

[0059] 100ml solution, its components are 4mol / L NaCl solution, 0.5ml Tris-HCl, pH=6.4, 45mmol / l EDTA, 2.5% Triton;

[0060] 100ml washing liquid I, its composition is 4mol / l NaClO 4 Solution, 0.5mol / l Tris-HCl, pH=6.7, 25mmol / l EDTA;

[0061] 10ml eluent, the composition of which is 10mmol / l Tris-HCl.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a recycling method of DNA and agent box through magnetic nanometer composite material in the biological technical domain, which comprises the following steps: (a) blending magnetic nanometer composite material and recycled sample; forming solid-liquid dispersing suspension; (b) adsorbing DNA to separate magnetic nanometer composite material from solid phase; obtaining magnetic nanometer composite material with adsorbed DNA; (c) washing impurity; evolving DNA from magnetic nanometer composite material. The magnetic nanometer composite agent box to recycle DNA possesses magnetic nanometer composite material solution, dissolving liquid, washing liquid I and washing liquid II and eluent.

Description

technical field [0001] The present invention relates to the field of biotechnology. More specifically, the present invention relates to a method for recovering DNA using magnetic nanoparticles and a kit for recovering DNA. Background technique [0002] The recovery of DNA has a wide range of applications, such as recovery of high-purity DNA from DNA polymerase chain reaction (PCR) amplification products, polyacrylamide gel, agarose gel, digested biological samples for genetic fingerprint identification, gene Sequencing, library construction, PCR testing, etc. DNA recovery technology is a key technology in molecular biology experiments. The efficiency and purity of recovered DNA will directly affect the success of subsequent experiments. Therefore, developing fast, efficient, simple and low-cost DNA recovery methods has always One of the goals of academics. [0003] At present, the main DNA recovery methods are chemical method, glass bead method and column separation metho...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/10
Inventor 杨百全王刚杨文胜
Owner JILIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap