3 results about "Dna recovery" patented technology

The invention belongs to the field of a trace sample nucleic acid extraction method, and discloses a fresh poultry egg high-efficiency DNA extraction method based on embryonic disc separation, the method adopts an acoustoelectric coupling micropore cracking-separation technology, the method comprises the following steps: synchronously applying 1.5 MHz high-frequency sound wave and a 2 kV / cm, 1 kHz alternating electric field in a micro-fluidic chip, generating controllable nano-scale micropores in an embryonic disc cell membrane, and separating the embryonic disc cell membrane by using an ultrasonic wave; the ultra-fast cracking within 500 microseconds is realized; split products are directionally screened through a polytetrafluoroethylene microporous membrane under the driving of an electric field, only DNA molecules are allowed to pass through, proteins and lipids are intercepted, and thermal damage and chemical residues are avoided; the method is suitable for 0.5 microliter ultramicro embryonic disc samples, and the DNA recovery rate is gt; the purity is 90%, and the purity is A260 / 280gt; 1.8, the requirements of high-throughput sequencing and SNP (Single Nucleotide Polymorphism) analysis are met.

The invention belongs to the technical field of biology, and particularly relates to a method for extracting metagenomes of microorganisms in a mud-water mixture. Comprising the following steps: S1, mud-water sample separation: separating mud and a water body part in mud-water; s2, extraction of metagenomes in the soil: extracting the metagenomes in the soil; s3, water body sample separation: separating microorganisms from free DNA in the water body; s4, extracting metagenomes of thallus samples in water, and extracting metagenomes of microorganisms in the water; and S5, extracting metagenome of the free DNA sample in water, and extracting free DNA in the water. According to the method disclosed by the invention, almost all microorganism DNAs can be effectively recovered from the mud-water mixture, and the DNA loss caused by sample separation is reduced. The DNA recovery rate is improved through optimized splitting decomposition and purification steps, and it is ensured that a high-quality DNA sample can be obtained even under extreme conditions.

The present application relates to the field of biotechnology and molecular diagnosis, and particularly relates to a methylation detection pretreatment kit and method for protecting DNA integrity, which can solve the problem that DNA degradation is serious in traditional bisulfite conversion technology, and conversion efficiency and DNA integrity are difficult to be obtained simultaneously, the kit comprises a conversion solution, a protection solution, a combination solution, a desulfonation solution, a washing solution and an elution solution, the conversion solution contains 2.5-4 mol / L bisulfite and linear polyethylene polyamine, the protection solution contains an organic denaturant and a polyphenol antioxidant, the DNA melting temperature is reduced by the organic denaturant, the linear polyethylene polyamine accelerates the conversion reaction, and the desulfonation system with low water activity is combined to complete the bisulfite conversion under mild conditions of 50-70 DEG C; the present application can realize the conversion efficiency of greater than 99.5% of unmethylated cytosine, greatly reduce DNA degradation, significantly improve the long fragment DNA recovery rate and the detection sensitivity of trace samples, and is suitable for the methylation detection of three generations of long read sequencing and clinical trace samples.