Bacillus cellulase gene fib1x suitable for rice straw returning to field

Abstract

The Bacillus cellulase gene FIB1X suitable for rice straw returning to the field belongs to the field of farming and cultivation technology. The cycle of using bacteria to treat straw is very short, usually it can be completed in a few days, so that air pollution caused by burning straw can be avoided. Various problems caused by other processing methods. Therefore, the hydrolysis circle experiment to measure the cellulase activity of all intestinal bacteria of Brachypterus sp. was found to have the strongest cellulase activity. Then, through the PCR amplification of the gene, DNA recovery, ligation reaction, E. coli heat shock transformation, E. coli plasmid DNA extraction, gene induction expression, protein electrophoresis detection, sequence determination and analysis, the Bacillus cellulase gene FIB1X was obtained. The nucleic acid sequence is Seq ID No: 1. Finally, the enzyme activity of the protein expressed by the Bacillus cellulase gene FIB1X was determined, and the result showed that the enzyme activity was relatively high.

Description

technical field [0001] The invention relates to a bacillus cellulase gene FIB1X suitable for rice straw returning to the field, and belongs to the technical field of farming and cultivation. Background technique [0002] Straw returning to the field is a measure of fertilizing soil fertility and increasing production that is generally valued in the world today. It can increase fertilizer and increase production while eliminating the air pollution caused by straw burning. Straw returning can increase soil organic matter, improve soil structure, loosen soil, increase porosity, reduce capacity, and promote microbial activity and root development of crops. Straw returning to the field has a significant effect on increasing fertilizer and production, and generally can increase production by 5% to 10%. However, if the method is not appropriate, it will also lead to an increase in soil pathogens, aggravation of crop diseases, and lack of seedlings (sick seedlings) and other undesir...

AI Technical Summary

Problems solved by technology

[0003] In order to overcome how to use the characteristics of short reproduction cycle and easy operation of bacteria, we searched for suitable bacteria from the intestinal tract of some herbivorous Orthoptera insects, found the gene whose expressed protein has cellulase activity, and determined that the expressed protein of the gene has fiber The enzyme activity, the problem of applying bacteria to straw returning to the field to quickly decompose the cellulose in the straw, the present invention provides a Bacillus cellulase gene FIB1X suitable for rice straw returning to the field, suitable for the spore of rice straw returning to the field Bacillus cellulase gene FIB1X uses bacteria to treat straw in a very short cycle, usually within a few days, so as to avoid air pollution caused by straw burning and various problems caused by other treatment methods

Images