Agarose gel electrophoresis product recovering method

Abstract

The invention relates to an agarose gel electrophoresis product recovering method, which prevents experimenters from touching gel repeatedly, reduces rate of contamination of DNA (deoxyribonucleic acid), improves DNA recovery efficiency, shortens time of gel exposed under ultraviolet light, and reduces subsequent experiment risks caused by mutagenesis and damages of nucleic acid samples during ultraviolet excitation. A device utilizing the agarose gel electrophoresis product recovering method is unique in design, easy in manufacturing process, skillful, portable, simple and convenient in use and low in price.

Description

technical field [0001] The invention belongs to the field of chemistry, and relates to an electrophoresis recovery device, in particular to a method for recovering agarose gel electrophoresis products. Background technique [0002] Generally, in various molecular biology laboratories, DNA recovery from agarose gel is a routine experimental operation. It is the basis for gene cloning, sequencing and transgenic work. For example, specific fragments can be collected for cloning or probe preparation, and PCR products can be recovered for re-identification, etc. It took 1-2 hours to recover the gel electrophoresis fragments in the early stage, and then major brands have launched a variety of fast gel recovery kits with different purposes and different prices, which shortened the gel recovery time to more than 10 hours at once. Minutes, the operation is also greatly simplified. The two most important technical indicators in the recovery experiment are purity and recovery rate: ...

AI Technical Summary

Problems solved by technology

[0005] 1. In the process of moving the glue away from the UV lamp, it is necessary to contact the glue many times, which not only easily causes the fragmentation and damage of the glue in the process, but also increases the chance of contaminating the DNA product, and more importantly, the EB dye Harmful, this harmfulness is manifested in three aspects: contact carcinogenicity, mutagenicity of ultraviolet observation, and troublesome post-processing

But when it comes to EB substitutes, it seems that they are not so desirable. For example, SYBR dyes are unstable and easy to degrade under ultraviolet light. Some dyes such as GelRed have low toxicity, but they are expensive.

[0006] 2. After moving away from the UV lamp, perform blind cutting based on the memory of previous observations. Although the shortcomings mentioned in the appeal method can be avoided, the fatal thing is that it is easy to cause omissions in product recovery and is inaccurate.

Long-term exposure to strong ultraviolet rays can cause blindness

[0009] 2. When this method is operated, it is necessary to place the glue under ultraviolet light for a longer time, which increases the mutagenesis and damage to the nucleic acid sample caused by ultraviolet excitation, so that the cloning efficiency is low, and even the expression protein error and Mutations seriously affect subsequent experiments

[0011] 1. Most professional glue cutters are expensive, which has great limitations in their popularization and promotion;

[0012] 2. Most of the rubber cutters are complex in structure, requiring professional personnel to install and maintain, and the installation and maintenance costs are high;

[0013] 3. There are certain requirements for the installation conditions of the laboratory, and not every laboratory can be equipped with equipment;

[0014] 4. The volume is huge and inconvenient to carry, and the operation is relatively complicated

[0015] Therefore, experimenters urgently need a kind of agarose gel electrophoresis recovery device that can not only protect their own safety, but also improve test efficiency, and is cheap. see the report

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