4 results about "DNA Contamination" patented technology

The invention discloses a blocking agent for eliminating environmental DNA (Deoxyribose Nucleic Acid) of human pollution and a detection method of the environmental DNA, and the blocking agent plays a role in inhibiting amplification of a human source sequence by specifically hybridizing with a human source template and blocking primer annealing or DNA polymerase extension. The invention further provides application of the CRISPR-Cas12a technology to in-vitro removal of human-derived DNA pollution, the method can reduce the sequencing cost and the time cost of bioinformatics processing without affecting detection of target biological groups, and the method is mainly used for treating human pollution in marine environment DNA. And the method can also be used for treating human-derived DNA pollution in similar molecular biology research and application.

This invention provides a method for detecting genome-wide ROH based on ultra-low depth sequencing. The method first acquires the raw PGT-A sequencing data of the sample, extracts high-frequency SNP loci from the population, and screens out loci on autosomes with sequencing depths of 2-4X to construct a locus set. Then, the heterozygosity index (HetRR) and its standard score (Z) are calculated. Based on thresholds, the results are judged as follows: HetRR < 0.1 and Z ≤ -3.0 indicates genome-wide ROH; 0.1 ≤ HetRR < 0.5 and Z ≤ -3.0 indicates ROH chimerism; HetRR ≥ 1.05 and Z ≥ 3.0 suggests triploidy or DNA contamination; the rest are normal. This method can utilize sequencing data as low as 0.01X and can be simultaneously detected in the routine PGT-A process without additional operations or costs, improving the accuracy of embryo screening and possessing high clinical application value.

Provided are an amplification-free method for the preimplantation genetic testing (PGT) of in-vitro fertilization (IVF) embryos, an amplification-free method of generating a library for the PGT, and a method of identifying the genetic background of IVF embryos. Provided are also a method of determining the degree of non-embryonic DNA contamination, and an amplification-free method of generating a library of cell-free DNAs (cfDNAs) from a biological material. Similarly provided are a method for enrichment of methylated fragments from the amplification free library, and a method for identifying methylation profiles and / or changes of same, in samples of different sources.

The invention relates to a plasmid target spot, a primer probe, a kit and a method for detecting host DNA residues in a cell preparation or a virus preparation. The invention relates to the technical field of biology, in particular to a plasmid target spot, a primer probe, a kit and a method for detecting host DNA residues in a cell preparation or a virus preparation. The plasmid target point is selected from one or two of plasmid Ori and KanR genes, the Ori element has a gene sequence as shown in SEQ ID NO.1, the KanR gene has a gene sequence capable of coding a Kan R peptide chain, such as a nucleotide sequence for coding SEQ ID NO.2, the nucleotide sequence can have any one nucleotide sequence in SEQ ID NO.3-5, and the Kan R has an amino acid sequence as shown in SEQ ID NO.2. The invention also relates to a primer probe of the plasmid target, a kit and a use method. The invention provides an effective detection target (Ori and KanR genes) and an accurate detection amplification system, and detection of host DNA residues and plasmid DNA residues in a cell preparation or a virus preparation is realized.