Micro-blood RNA (ribonucleic acid) quick separation method through nucleic acid adsorption

A nucleic acid and blood technology, applied in DNA preparation, recombinant DNA technology, etc., can solve the problems of complicated operation, impurity, and time-consuming, and achieve the effects of high RNA purity, small sampling amount, and easy operation.

Inactive Publication Date: 2013-01-09
西安交通大学口腔医院
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Problems solved by technology

Because the isolation of mononuclear cells requires a large amount of blood, it brings difficulties and inconvenience to sampling, and more importantly, it brings unnecessary pain and psychological burden to patients, and affects and limits clinical detection, diagnosis and research of diseases.
In addition, because blood contains a large amount of RNase and some components in red blood cells, if the treatment is not clean, it will seriously affect the quality of RNA extraction and the success of subsequent experiments. Traditional methods of extracting RNA include using lymphocyte separation fluid. This is time-consuming and cumbersome
This is also an important reason that affects the easy degradation and low yield and impurity of RNA extraction in blood.

Method used

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  • Micro-blood RNA (ribonucleic acid) quick separation method through nucleic acid adsorption

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Embodiment Construction

[0016] According to the technical scheme of the present invention, the rapid separation method for nucleic acid adsorption of trace blood RNA of the present invention takes a short time in the whole experimental process, and can effectively inhibit the activity of RNase, and with the addition of specific adsorption, the extracted RNA does not degrade, and the activity High, high purity.

[0017] The nucleic acid adsorption centrifuge sleeve includes an inner adsorption tube and an outer collection tube, wherein the inner adsorption tube contains an adsorption medium layer, and the outer sleeve is a liquid collection tube. When adding reagents, the inner adsorption tube needs to be inserted into the outer collection tube, discard the waste liquid in the collection tube after centrifugation, and then return the adsorption tube to the collection tube. When adding the separation liquid at the end, insert the adsorption tube into a clean (new) collection tube , and then centrifuged...

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Abstract

The invention discloses a micro-blood RNA (ribonucleic acid) quick separation method through nucleic acid adsorption. A nucleic acid adsorption centrifuge shield and RNA extraction reagent are used for quickly separating RNA from micro-blood, wherein the RNA extraction reagent consists of red blood cell lysis solution, dissociation solution, removing solution, washing solution and separation solution. The micro-blood RNA quick separation method through nucleic acid adsorption has the advantages that the method is simple and convenient to operate, the time is short, the sampled quantity is small, the purity of the extracted RNA is high, the RNA is not polluted by protein and DNA (deoxyribonucleic acid), the superiority to the traditional blood RNA extraction method is shown, and application values of clinical popularization of the method for disease diagnoses and researches are further represented.

Description

technical field [0001] The invention belongs to the blood RNA separation method in the technical field of molecular biology, and specifically relates to the rapid separation of RNA from a small amount of blood. The combined application of a specific RNA separation reagent and a nucleic acid adsorption centrifuge sleeve can quickly separate the RNA in the blood, and the isolated The RNA yield and purity are high, without protein and genomic DNA contamination, and the operation is simple and time-consuming. Background technique [0002] At present, for the extraction of RNA in blood at home and abroad, lymphocyte separation medium is often used to separate mononuclear cells first, and then lyse and extract. Because the isolation of mononuclear cells requires a large amount of blood, it brings difficulties and inconvenience to sampling, and more importantly, it brings unnecessary pain and psychological burden to patients, and affects and limits clinical detection, diagnosis and...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 饶国洲李昂李晓红周洪
Owner 西安交通大学口腔医院
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