Method for extracting ancient human remains matter DNA

An extraction method and material technology, which is applied in the field of DNA extraction from ancient human remains, can solve the problems of false positives, inability to effectively remove lysate proteins, organic reagents, and low success rate, and achieve the effect of increasing the content
CN1821265AInactive Publication Date: 2006-08-23XI AN JIAOTONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
XI AN JIAOTONG UNIV
Publication Date
2006-08-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to method of extracting ancient human remains matter DNA. The method includes first treating skeleton sample with plasma to decompose the surface pollutant and subsequent DNA extraction. The DNA extraction includes the steps of polishing skeleton sample, hypochloric acid treatment, ultraviolet irradiation, alcohol solution washing to eliminate foreign microbe contamination, Ar / O2 (9:1) plasma treatment, grinding in liquid nitrogen to obtain powder sample; digesting and cracking sample, phenol-chloroform extraction to eliminate digested organic molecule, adsorption with silica adsorption column, and filtering in 3 KDa ultrafiltering centrifugation tube to obtain ancient human remains matter DNA. The method of the present invention can eliminate surface pollutant fast effectively, and has high ancient human remains matter DNA extracting success rate and high template content.
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Description

technical field

[0001] The invention relates to the extraction and purification of ancient human DNA, in particular to a method for extracting ancient human DNA, which can prevent endogenous and exogenous pollution and improve the success rate of extraction. Background technique

[0002] At present, ancient DNA extraction methods are mainly based on some extraction methods in forensic science. These extraction schemes generally first remove the exogenous DNA on the sample surface by polishing the sample surface or sawing off the sample surface by 1-2mm with a wire saw, ultraviolet irradiation, and washing with 70% ethanol. contamination, and then grind the sample into powder in liquid nitrogen for pre-sample treatment. Specifically, there are mainly three different extraction schemes: proteinase K / phenol-chloroform method, silica method and guanidine isothiocyanate-silica method.

[0003] 1. The step of proteinase K / phenol-chloroform method (ThomasWK...

Claims

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