11 results about "Human dna" patented technology

The application discloses a human DNA sample quantitative amplification system and application thereof, and the amplification system comprises a primer and probe compound for specifically amplifying four target sequences; wherein the four target sequences are two autosomal target genes CSF1PO and TPOX, one Y chromosome target gene SRY and one non-human synthetic oligonucleotide IPC. Advantages are as follows: (1) the amplification system and the kit thereof can be applied to all forensic DNA analysis, four different target genes can be simultaneously amplified in one tube, human total DNA and human male DNA can be quantitatively and qualitatively evaluated, and information about sample degradation and inhibitor condition is provided. The primer and probe are high in specificity and sensitivity, and the minimum detection limit can reach 1 pg level. (2) the kit effectively simplifies the steps of qPCR reaction operation, improves the accuracy and precision of the test, and is suitable for all collected sample sources in forensic DNA analysis.

The invention discloses heat-resistant saccharomyces cerevisiae of a heterozygous human DNA methylation system as well as a construction method and application of the heat-resistant saccharomyces cerevisiae. The construction method comprises the following steps: determining a to-be-knocked-in saccharomyces cerevisiae target gene locus CAN1, and carrying out annular PCR amplification by taking a target sgRNA sequence as a homologous arm and an original gRNA plasmid as a template to obtain a gRNA plasmid; constructing a to-be-knocked-in gene segment, and transferring the constructed gRNA plasmid, the segment 1 and the segment 2 into BY4741-Cas9 saccharomycetes through a saccharomyces cerevisiae lithium acetate conversion method; the CRISPR plasmid is lost by adopting an iteration method to obtain a saccharomyces cerevisiae strain which is integrated into a genome and has DNA methylation modification, and the saccharomyces cerevisiae strain is named as yHL006. The invention constructs a saccharomyces cerevisiae chassis strain capable of stably expressing a DNA methylation system. Any auxotrophic selection marker is not occupied, and the complete amino acid synthesis capability and corresponding gene loci of the yeast are completely reserved. The heat stress resistance phenotype of the DNA methylation strain is verified, and the heat resistance of the yeast can be improved by the introduced DNA methylation system.

The present invention provides nucleic acid molecules and methods that use miRNA sequences that knocking down mRNA for the human DNA mismatch repair protein MutS Homolog 3 (MSH3), thereby resulting in the treatment of Huntington's Disease.

Compositions, kits, and methods are provided for validating patient-specific oligonuclotide assays using spike-in controls. The compositions comprise multiple sets of spike-in oligos, each set including a unique reference sequence and near-neighbor sequences with at least one substitution, insertion, or deletion. Near-neighbor concentrations are less than the reference and collectively span at least four orders of magnitude (e.g., 1:10 to 1:10,000); all sets have the same number of near-neighbors and concentration ratios. Spike-in sequences are unrelated to human sequences and are complementary to synthesis-control oligos at known concentrations. Kits further include synthesis-control oligos and patient-specific oligos for human DNA or RNA targets. Methods involve spiking a sample, co-synthesizing control and patient-specific oligos, assaying by PCR or sequencing, and validating limit of detection and dynamic range by comparing observed counts to known ratios. Applications include ctDNA and cfRNA testing.

The invention discloses a multiple qPCR (quantitative polymerase chain reaction) detection composition, method and kit for detecting various DNA / RNA (deoxyribonucleic acid / ribonucleic acid) viruses, and aims to detect various human DNA / RNA viruses possibly existing in biological products, compare and analyze through database analysis, find virus genome conserved sequences, design primers and probes with higher specificity and detect most subtypes of the viruses. A plurality of pairs of probe primers are designed for a virus conserved region, the primer probes with good specificity and high sensitivity are finally screened, the virus content of 2 * 10 < 7 > copies / [mu] L to 20 copies / [mu] L can be accurately quantified, and the sensitivity of most viruses can reach 2-10 copies / [mu] L. By repeatedly screening and optimizing a reaction system, including the concentrations of the primers and the probes, the detection method can be used for stably detecting various DNA / RNA viruses under the condition of fewer nucleic acid samples, is simple to operate and has higher sensitivity.

A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.

1. The name of the design product: kit. 2. The use of the design product: the design product is used for the packaging and transportation of human DNA typing detection reagents. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view. 5. Other circumstances that need to be explained: see the reference drawing for one of the unfolded forms.

This invention belongs to the field of molecular biology technology, specifically relating to a method for high-purity extraction of human fecal DNA based on secondary magnetic bead purification. The method involves first performing preliminary lysis of the fecal supernatant, then using coarse magnetic beads to bind nucleic acids in the sample. After preliminary rinsing and elution, the coarse magnetic beads and nucleic acids are eluted into a first elution buffer. Next, using the first elution buffer as the sample, gentle lysis is performed, followed by the use of fine magnetic beads to bind nucleic acids in the sample. Further rinsing and elution yield high-purity fecal human DNA. The obtained high-purity DNA exhibits low inhibition and high purity, and can be directly used in downstream detection experiments.

The present invention provides nucleic acid molecules and methods that use miRNA sequences that knocking down mRNA for the human DNA mismatch repair protein MutS Homolog 3 (MSH3), thereby resulting in the treatment of Huntington's Disease.

The invention relates to a preparation method of a standard substance for human DNA STR detection, and belongs to the technical field of gene detection service. According to the method, a representative allele combination of 20 core STR loci of a CODIS system is optimized and determined according to STR genetic characteristics of people; chemically synthesizing a double-stranded DNA fragment containing a target STR repeat region and upstream and downstream flanking sequences of a natural genome; mixing according to an equal molar ratio after adopting a high-precision quantitative technology; and adding a composite freeze-drying protective additive consisting of trehalose and glycerol, and preparing the freeze-dried powder standard substance through an optimized freeze-drying process. According to the invention, fully synthesized DNA is adopted to replace a human source sample, ethical and privacy risks are thoroughly avoided, and excellent inter-batch consistency and room-temperature long-term stability are realized. The prepared standard substance highly accords with the genetic characteristics of Chinese population, and the coverage degree is obviously higher than the international general standard.

The invention relates to the production of all nitrogen atom-labeled internal standard products of Poly [ADP-ribose] polymerase 1 (PARP1) and 8-oxoguanine-DNA glycosylase (OGG1), which are DNA repair proteins.