26 results about "Human dna" patented technology

The invention relates to a vessel for the long-term storage of DNA in space at a constant temperature of -196 °C. The aim is to protect biological samples – especially DNA – for decades to centuries from the extreme environmental conditions of space, including vacuum, intense radiation, extreme temperature fluctuations and mechanical stresses. The vessel has a three-layered structure: 1. Inner chamber made of boron nitrogen nanotubes (BNNTs) that offer outstanding protection against cosmic radiation, high mechanical strength, chemical inertness and temperature resistance. 2. Middle insulating layer made of aerogel that minimizes heat exchange and keeps the cryogenic temperature constant. 3. Outer protective layer made of titanium, which protects against micrometeorites, It protects against physical impacts and corrosion. Technical advantages: • Long-term stability of DNA through consistently low temperatures. • Effective radiation protection against cosmic and solar radiation. • Excellent thermal insulation thanks to aerogel. • Mechanical robustness due to titanium. Areas of application: • Securing and archiving genetic material for research, space travel and long-term preservation. • Provision of DNA for future scientific or biological restoration projects. Special feature: The capsule is compact, space-compatible, and can be used as part of space missions, space stations, or interplanetary projects.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer system for improving on-site detection accuracy, a freeze-drying reagent ball and application, and belongs to the technical field of molecular biology. The LAMP primer system comprises a primer combination for detecting human DNA (Deoxyribonucleic Acid) and a primer combination for detecting gender DNA, wherein the primer combination for detecting the sex DNA is designed based on an SRY gene coding region and a non-coding region, and the sequence of the primer combination is as shown in SEQ ID NO.1-12; and the sequences of the primer combination for detecting the human DNA are shown as SEQ ID NO. 13-18. A set of LAMP primers is designed on the basis of partial sequences on the Cyt b gene and the SRY gene, and the sensitivity of the constructed system can reach 25pg. The whole detection process is easy to operate, consumed time is short, closed treatment and information acquisition of the sample can be completed within 40 minutes, and a whole set of rapid, accurate and sensitive detection scheme is provided for forensic field rapid detection.

The application discloses a human DNA sample quantitative amplification system and application thereof, and the amplification system comprises a primer and probe compound for specifically amplifying four target sequences; wherein the four target sequences are two autosomal target genes CSF1PO and TPOX, one Y chromosome target gene SRY and one non-human synthetic oligonucleotide IPC. Advantages are as follows: (1) the amplification system and the kit thereof can be applied to all forensic DNA analysis, four different target genes can be simultaneously amplified in one tube, human total DNA and human male DNA can be quantitatively and qualitatively evaluated, and information about sample degradation and inhibitor condition is provided. The primer and probe are high in specificity and sensitivity, and the minimum detection limit can reach 1 pg level. (2) the kit effectively simplifies the steps of qPCR reaction operation, improves the accuracy and precision of the test, and is suitable for all collected sample sources in forensic DNA analysis.

The invention discloses heat-resistant saccharomyces cerevisiae of a heterozygous human DNA methylation system as well as a construction method and application of the heat-resistant saccharomyces cerevisiae. The construction method comprises the following steps: determining a to-be-knocked-in saccharomyces cerevisiae target gene locus CAN1, and carrying out annular PCR amplification by taking a target sgRNA sequence as a homologous arm and an original gRNA plasmid as a template to obtain a gRNA plasmid; constructing a to-be-knocked-in gene segment, and transferring the constructed gRNA plasmid, the segment 1 and the segment 2 into BY4741-Cas9 saccharomycetes through a saccharomyces cerevisiae lithium acetate conversion method; the CRISPR plasmid is lost by adopting an iteration method to obtain a saccharomyces cerevisiae strain which is integrated into a genome and has DNA methylation modification, and the saccharomyces cerevisiae strain is named as yHL006. The invention constructs a saccharomyces cerevisiae chassis strain capable of stably expressing a DNA methylation system. Any auxotrophic selection marker is not occupied, and the complete amino acid synthesis capability and corresponding gene loci of the yeast are completely reserved. The heat stress resistance phenotype of the DNA methylation strain is verified, and the heat resistance of the yeast can be improved by the introduced DNA methylation system.

The present invention includes antibodies and antigen-binding fragments thereof that specifically bind a double-stranded deoxyribonucleic acid (DNA) strand. Polynucleotides encoding an antibody or an antigen-binding fragment, vectors, and host cells that comprise a polynucleotide and pharmaceutical compositions are also included in this invention. Also described herein are methods of using the presently disclosed antibodies, antigen-binding fragments, polynucleotides, vectors, host cells, and compositions to diagnose, prevent or treat a DNA virus infection.

The present invention provides nucleic acid molecules and methods that use miRNA sequences that knocking down mRNA for the human DNA mismatch repair protein MutS Homolog 3 (MSH3), thereby resulting in the treatment of Huntington's Disease.

A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.

Compositions, kits, and methods are provided for validating patient-specific oligonuclotide assays using spike-in controls. The compositions comprise multiple sets of spike-in oligos, each set including a unique reference sequence and near-neighbor sequences with at least one substitution, insertion, or deletion. Near-neighbor concentrations are less than the reference and collectively span at least four orders of magnitude (e.g., 1:10 to 1:10,000); all sets have the same number of near-neighbors and concentration ratios. Spike-in sequences are unrelated to human sequences and are complementary to synthesis-control oligos at known concentrations. Kits further include synthesis-control oligos and patient-specific oligos for human DNA or RNA targets. Methods involve spiking a sample, co-synthesizing control and patient-specific oligos, assaying by PCR or sequencing, and validating limit of detection and dynamic range by comparing observed counts to known ratios. Applications include ctDNA and cfRNA testing.

The present invention includes antibodies and antigen-binding fragments thereof that specifically bind a double-stranded deoxyribonucleic acid (DNA) strand. Polynucleotides encoding an antibody or an antigen-binding fragment, vectors, and host cells that comprise a polynucleotide and pharmaceutical compositions are also included in this invention. Also described herein are methods of using the presently disclosed antibodies, antigen-binding fragments, polynucleotides, vectors, host cells, and compositions to diagnose, prevent or treat a DNA virus infection.

The invention discloses a multiple qPCR (quantitative polymerase chain reaction) detection composition, method and kit for detecting various DNA / RNA (deoxyribonucleic acid / ribonucleic acid) viruses, and aims to detect various human DNA / RNA viruses possibly existing in biological products, compare and analyze through database analysis, find virus genome conserved sequences, design primers and probes with higher specificity and detect most subtypes of the viruses. A plurality of pairs of probe primers are designed for a virus conserved region, the primer probes with good specificity and high sensitivity are finally screened, the virus content of 2 * 10 < 7 > copies / [mu] L to 20 copies / [mu] L can be accurately quantified, and the sensitivity of most viruses can reach 2-10 copies / [mu] L. By repeatedly screening and optimizing a reaction system, including the concentrations of the primers and the probes, the detection method can be used for stably detecting various DNA / RNA viruses under the condition of fewer nucleic acid samples, is simple to operate and has higher sensitivity.

A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.

Pharmaceutical composition in the form of a capsule for oral administration comprising a encapsulated processed faecal composition that comprises faecal microbiota, characterized in that, the processed faecal composition is a powdered lyophilized faecal composition that is encapsulated with a coating shell which is a pH responsive polymer composition and may dissolve or provide a delayed release of the processed faecal composition to the intestine, and wherein the processed faecal composition comprises faecal microbiota comprising colony forming units (CPU) and equal to or less than 1% of human DNA with respect to the total DNA. Method of preparation of the pharmaceutical composition.

A complex nanostructure, which includes a first nanostructure component having at least one aperture in a side thereof; at least one second nanostructure component having a first end and a second end, wherein the first end of each of the at least one second nanostructure is inserted through a corresponding one of the at least one aperture in the first nanostructure, thereby forming at least one junction. Embodiments of the complex nanostructure include a bifurcated nanostructure transistor constructed of linear carbon nanotubes, a multiplexer constructed of a circular carbon nanotube and multiple linear carbon nanotubes, and an information unfolder constructed of linear or a combination of linear and circular carbon nanotubes. The nanotubes may optionally be decorated with genetic material such as single-strand or double-strand human DNA segments and / or may be modified by e-beam or ozone gas to add defects into the nanotubes to alter electrical / functional characteristics.

A complex nanostructure, which includes a first nanostructure component having at least one aperture in a side thereof; at least one second nanostructure component having a first end and a second end, wherein the first end of each of the at least one second nanostructure is inserted through a corresponding one of the at least one aperture in the first nanostructure, thereby forming at least one junction. Embodiments of the complex nanostructure include a bifurcated nanostructure transistor constructed of linear carbon nanotubes, a multiplexer constructed of a circular carbon nanotube and multiple linear carbon nanotubes, and an information unfolder constructed of linear or a combination of linear and circular carbon nanotubes. The nanotubes may optionally be decorated with genetic material such as single-strand or double-strand human DNA segments and / or may be modified by e-beam or ozone gas to add defects into the nanotubes to alter electrical / functional characteristics.

1. The name of the design product: kit. 2. The use of the design product: the design product is used for the packaging and transportation of human DNA typing detection reagents. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view. 5. Other circumstances that need to be explained: see the reference drawing for one of the unfolded forms.

The present invention discloses a DNA methylation site prediction method based on secondary fine-tuning and an integrated algorithm, belonging to the field of bioinformatics technology. The present invention uses human DNA promoter sequences obtained from the UCSC database, after 1-mer, 3-mer, and 5-mer segmentation, as a corpus to pre-train the BERT model, forming a Promoter-BERT model. This model captures and abstracts deep features in DNA promoter sequences, providing an efficient and robust initial state for subsequent fine-tuning. The Promoter-BERT model is first fine-tuned using the three largest datasets of three methylation types, and then fine-tuned again using 14 datasets with smaller data volumes. This allows the model to focus more on learning the unique features of the dataset, thereby better adapting to specific tasks. This helps the model achieve higher accuracy and performance on the target task and reduces the risk of overfitting.

This invention belongs to the field of molecular biology technology, specifically relating to a method for high-purity extraction of human fecal DNA based on secondary magnetic bead purification. The method involves first performing preliminary lysis of the fecal supernatant, then using coarse magnetic beads to bind nucleic acids in the sample. After preliminary rinsing and elution, the coarse magnetic beads and nucleic acids are eluted into a first elution buffer. Next, using the first elution buffer as the sample, gentle lysis is performed, followed by the use of fine magnetic beads to bind nucleic acids in the sample. Further rinsing and elution yield high-purity fecal human DNA. The obtained high-purity DNA exhibits low inhibition and high purity, and can be directly used in downstream detection experiments.

The invention relates to the technical field of biological detection, and particularly discloses an MIRA constant-temperature kit for synchronously detecting mycoplasma and human DNA and a detection method of the MIRA constant-temperature kit, high-sensitivity, high-specificity and high-repeatability single-tube double-target detection is realized by designing specific primers and exo type fluorescent probes aiming at a mycoplasma CARDS gene and a human POP7 gene; according to the method, amplification can be completed within 20 min under the constant temperature condition, the sample quality and the mycoplasma infection condition are synchronously verified through real-time dual-channel fluorescence monitoring, and a false negative result is effectively avoided; the method is easy and convenient to operate, rapid in detection and suitable for rapid nucleic acid detection in clinical, on-site and resource-limited places.

The present invention discloses a precise gene editing method with high efficiency and low by-products. The method of the present invention utilizes an enzyme (such as FnCas12a) that can cleave DNA and generate strict sticky ends, fuses a high-fidelity ligase (such as Human DNA Ligase III), and forms a multimer in the form of (FnCas12a-ligase) n by interacting proteins, co-transfects cells with a donor plasmid capable of generating corresponding sticky ends, achieves efficient and precise gene editing, and simultaneously eliminates accidental insertions or deletions. The method involved in the present invention has extremely low cytotoxicity to cells, can be repeatedly performed in multiple rounds of editing to further improve the efficiency of precise gene editing to a greater extent. The precise gene editing method involved in the present invention is applicable not only to dividing cells but also to non-dividing cells.

The present invention discloses a multiplex amplification system for forensic human DNA detection by qPCR and its application. The multiplex amplification system simultaneously amplifies four targets, namely HS, HL, HY and IPC, by qPCR. Among them, the HS target is used for total DNA quantification, the HL target is used for degradation degree evaluation, the HY target is used for sex ratio judgment, and the IPC target is used for inhibitor detection. The kit of the present invention detects four targets, namely HS, HL, HY and IPC, by qPCR technology to achieve DNA quantification and quality analysis, and the operation is simple. It has high sensitivity (the lowest detection limit is 1 pg / μL), strong anti-inhibition ability, good specificity, can accurately quantify male and female DNA, can also evaluate the DNA degradation degree, judge PCR inhibitors, is applicable to a variety of biological samples, provides an efficient and accurate solution for forensic DNA detection, and helps solve cases.

A complex nanostructure, which includes a first nanostructure component having at least one aperture in a side thereof; at least one second nanostructure component having a first end and a second end, wherein the first end of each of the at least one second nanostructure is inserted through a corresponding one of the at least one aperture in the first nanostructure, thereby forming at least one junction. Embodiments of the complex nanostructure include a bifurcated nanostructure transistor constructed of linear carbon nanotubes, a multiplexer constructed of a circular carbon nanotube and multiple linear carbon nanotubes, and an information unfolder constructed of linear or a combination of linear and circular carbon nanotubes. The nanotubes may optionally be decorated with genetic material such as single-strand or double-strand human DNA segments and / or may be modified by e-beam or ozone gas to add defects into the nanotubes to alter electrical / functional characteristics.

The present invention provides nucleic acid molecules and methods that use miRNA sequences that knocking down mRNA for the human DNA mismatch repair protein MutS Homolog 3 (MSH3), thereby resulting in the treatment of Huntington's Disease.

The present invention discloses a primer combination for detecting 6 pathogenic microorganisms and their 20 drug resistance genes, including upstream primer F1: 5’-GAACGACATGGCTACGATCCGACTT SPECIAL F-3’; downstream primer R1: 5’-CTAAGACCGCTTGGCCTCCGACTT SPECIAL R-3’, wherein SPECIAL F includes the odd-numbered sequences among sequences 1-64 in the sequence listing, and SPECIAL R includes the even-numbered sequences among sequences 1-64 in the sequence listing. The purpose of the present invention is to provide a pathogenic microorganism amplicon targeted capture sequencing technology, aiming to enrich the microbial genomes with low starting amounts after selectively depleting human DNA from clinical specimens, simply and effectively improving the detection rate of pathogenic microorganisms and at the same time increasing the detection rate of their drug resistance genes.

The invention discloses a primer, a probe, an implementation method and a detection system for qPCR detection of SQLE drug-resistant mutation of dermatophytes, and the method comprises the following steps: identifying a plurality of key mutation sites related to drug resistance by comparing an obtained dermatophytes SQLE gene sequence with a reference sequence in an NCBI database; designing a specific primer, a TaqMan-MGB fluorescent probe and a wild type closed probe modified by the MGB probe based on the mutation site, and verifying the sensitivity and the specificity through the strain level; the method comprises the following steps: constructing a mixed DNA sample model containing drug-resistant strain DNA, wild type strain DNA and human DNA, optimizing a reaction system of single and multiple qPCR detection, and determining an optimal reaction system of qPCR detection. According to the specific primer and probe for qPCR detection of dermatophyte SQLE drug-resistant mutation with specificity meeting requirements and an optimized reaction system for qPCR detection, whether dermatophyte SQLE drug-resistant mutation exists or not can be quickly verified, the sensitivity and specificity of dermatophyte drug-resistant detection are improved, early precise treatment is facilitated, and prognosis of patients is improved.

The invention relates to a preparation method of a standard substance for human DNA STR detection, and belongs to the technical field of gene detection service. According to the method, a representative allele combination of 20 core STR loci of a CODIS system is optimized and determined according to STR genetic characteristics of people; chemically synthesizing a double-stranded DNA fragment containing a target STR repeat region and upstream and downstream flanking sequences of a natural genome; mixing according to an equal molar ratio after adopting a high-precision quantitative technology; and adding a composite freeze-drying protective additive consisting of trehalose and glycerol, and preparing the freeze-dried powder standard substance through an optimized freeze-drying process. According to the invention, fully synthesized DNA is adopted to replace a human source sample, ethical and privacy risks are thoroughly avoided, and excellent inter-batch consistency and room-temperature long-term stability are realized. The prepared standard substance highly accords with the genetic characteristics of Chinese population, and the coverage degree is obviously higher than the international general standard.

The invention relates to the production of all nitrogen atom-labeled internal standard products of Poly [ADP-ribose] polymerase 1 (PARP1) and 8-oxoguanine-DNA glycosylase (OGG1), which are DNA repair proteins.