The invention discloses a multi-tumor combined detection kit. The multi-tumor combined detection kit comprises
fluorescent microspheres capable of performing
time resolution, a
signal amplification
system, a first tumor-associated
protein, a sample to-be-detected object, a second tumor-associated
protein and
polymer fluorescent coding microspheres. The invention further discloses a use method of the kit. The method comprises the following steps: adding a to-be-detected sample;
processing incubation oscillation, forming a reaction mixture of macromolecular
fluorescence codes, second tumor-associated
protein, a to-be-detected object, first tumor-associated protein and
fluorescent microspheres capable of being subjected to
time resolution; using a time-resolved flow type
fluorescence detection device for carrying out high-
throughput test; conveying sample liquid and sheath liquid to the flowing chamber together; after to-be-detected liquid particles sequentially pass through a flowing chamber capillary tube detection area, a first
laser is activating, enabling a first
laser fluorescence detector to collect fluorescence signals while a second
laser is activated, and after short-life
background fluorescence decay disappears, starting a second laser time-resolved fluorescence detection unit to collect signals for analysis and
processing.