11 results about "Structural motif" patented technology

A method of stabilizing a chemokine protein with a C-X-C or a C-C structural motif such as CXCL13 and CCL21 is provided. The method comprises the step of introducing the chemokine proteins into a solution comprising a buffer a non- ionic or amphoteric surfactant; and an aqueous chaotropic agent. A method of detecting a chemokine protein with a C-X-C or a C-C structural motif such as CXCL13 and CCL21 conjugated to antibodies thereto labelled with a fluorescent label in a solution is also provided. The method comprises the following steps: providing a test substrate comprising an elongate nitro cellulose membrane.

The invention relates to tryptanthrin derivatives with thiosemicarbazone substitution of the general formulae I and II, where R1-R8 are independently H, OH, alkyl with 1 to 6 carbon atoms, C(CH3)3, allyl, propargyl, ben-zyl, phenyl, F, Cl, Br, I, CH2OH, O (alkyl), CF3, OCF3, CN, COOH, COO(alkyl), CONH2, CONH(alkyl), NO2, N(alkyl)2, NH(alkyl), NHCO(alkyl), where the alkyl has 1 to 6 carbon atoms, or R1-R2 or R2-R3 or R3-R4 or R5-R6 or R6-R7 or R7-R8 is —CH═CH—CH═CH—, i.e., a fused benzene ring, X and Z are independently H, alkyl of 1 to 6 carbon atoms, benzyl or phenyl. The compounds combine a structural motif suitable for PLpro targeting, have strong affinity and selectivity for RNA over DNA, and at the same time effectively chelate ferric and ferrous ions. Thus, these compounds have the desired properties for potential use as inhibitors of the production of SARS-COV-2 viral particles and can thus be used for the preparation drugs for the treatment of coronavirus diseases, especially COVID-19.

Recombinant E. coli strains and synthetic protein sequence designs are leveraged for production of disordered polypeptides such as spidroins and elastin-like peptides (ELPs). These disordered polypeptides, the high-titer production of which has proven difficult, include repeating structural motifs from a small selection of amino acid residues, resulting in lack of well-defined tertiary and quaternary structure. The recombinant E. coli include expression vectors with genes encoding for the disordered polypeptide product. Expression of these genes is controlled by a promoter that downregulates and substantially inhibits basal expression in the recombinant bacteria. Further, the recombinant bacteria include mutations to one or more stress-response genes from wild-type E. coli, such as yggw, yedv, yedw, yedy, spec, speb, uspc, hcha, loip, mltc, envz, ompr, yhgf, or hupb. The recombinant E. coli enable production of high titers of disordered protein product while minimizing the toxic effects thereof on the host.

Heterocyclic compounds with a novel pyrazole thioamide-based NNSN structural motif, having highly effective zinc- or copper-activated toxicity against microbial infections at micromolar or nanomolar minimum inhibitory concentrations (MIC), and methods of making and using the same.

This invention belongs to the field of biotechnology and relates to a method for rapidly preparing three-dimensional self-assembled DNA crystals in a closed homogeneous system. The operation process of this invention is highly simplified, employing a "one-click annealing" mode. The reaction conditions are highly standardized, exhibiting excellent batch-to-batch reproducibility, and can stably obtain DNA crystals with regular morphology and uniform size, laying the foundation for subsequent large-scale preparation. More importantly, this strategy demonstrates good versatility and scalability, having been successfully validated on DNA structural motifs of different geometries and sizes (such as 2-turn and 4-turn stretched integral triangles). Its universality has been proven through two independent pathways: specific sequence design and chemical modification, indicating its potential to develop into a universal platform for DNA crystal construction.

Radical cascade reactions enabling sequence-controlled ring-closing polymerization and ring-opening polymerization for the controlled synthesis of polymers with complex main-chain structures are provided. Facile syntheses leading to low-strain macrocyclic monomers consisting of the ring-opening triggers and extended main-chain structures are also provided. The present disclosure further provides methods for excellent control over polymer molecular weights and molecular weight distributions and high chain-end fidelity allows for the preparation of polymeric systems with well-defined architectures. Further provided are the general nature of the radical cascade-triggered transformations in polymer chemistry, and its application to the synthesis of polymers with diverse main-chain structural motifs with tailored functions. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

The present disclosure discloses a programmatic design method for a topological protein, the method comprising the following steps: i) splitting an original structure of a protein-of-interest, and designing possible rewiring approaches according to a target topological structure; ii) evaluating connection approaches between structural motifs and determining priorities of the connection approaches in a subsequent design; iii) for each of the connection approaches, generating new virtual loop regions successively, exhausting all possible combinations of generation orders of the loop regions, creating corresponding spatial relationships, determining the formed chemical topological structures, calculating a formation probability of the target topological structure, and determining a length range of a newly-generated loop region; and iv) designing a length and sequence of the newly-generated loop region of the topological protein. The programmatic design method for topological proteins provided in the present disclosure provides a desirable platform for illustrating the structure-activity relationship of topological proteins and also provides a convenient method for developing functional topological proteins.

Recombinant E. coli strains and synthetic protein sequence designs are leveraged for production of disordered polypeptides such as spidroins and elastin-like peptides (ELPs). These disordered polypeptides, the high-titer production of which has proven difficult, include repeating structural motifs from a small selection of amino acid residues, resulting in lack of well-defined tertiary and quaternary structure. The recombinant E. coli include expression vectors with genes encoding for the disordered polypeptide product. Expression of these genes is controlled by a promoter that downregulates and substantially inhibits basal expression in the recombinant bacteria. Further, the recombinant bacteria include mutations to one or more stress-response genes from wild-type E. coli, such as yggw, yedv, yedw, yedy, spec, speb, uspc, hcha, loip, mltc, envz, ompr, yhgf, or hupb. The recombinant E. coli enable production of high titers of disordered protein product while minimizing the toxic effects thereof on the host.

The invention relates to a porous aromatic skeleton based on a tetraphenylmethane-aromatic imide-tetraphenylmethane dumbbell-shaped structural element as well as a preparation method and application of the porous aromatic skeleton. The porous aromatic skeleton takes tetraphenylmethane-aromatic imide-tetraphenylmethane with a hexabromo-substituted dumbbell-shaped structure as the structural element; the compound is obtained by performing Yamamoto coupling with a tetrabromo-substituted tetraphenylmethane structural element according to different molar ratios x, wherein the value range of x is 0-1. Compared with the prior art, the aromatic imide unit introduced into the structure endows the material with the characteristics of an organic porous material and a photoelectric functional material. Therefore, the material can be applied to the fields of adsorption separation of gas and micropollutants, photodegradation of pollutants, photocatalytic reaction and the like.

The present invention relates to a novel modified precursor of an RNA interference-inducing nucleic acid molecule, to a method for designing the modified precursor, and to a method for producing an RNA interference-inducing nucleic acid molecule. A modified precursor, according to one aspect, can be efficiently and homogeneously processed, and thus exhibits a higher expression level in cells, thereby being useful for design and regulation studies of RNA interference-inducing nucleic acid molecules.

The invention discloses a universal epegRNA eukaryotic expression skeleton vector as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The vector is an annular vector and comprises a pronucleus replication starting point, an ampicillin resistance gene, a U6 promoter, sgRNAscaffold, a 3'structural motif tevopreQ1, a TTTTTTT terminator, a BbsI restriction enzyme site, an LguI restriction enzyme site, an Mva1269I restriction enzyme site, an NheI restriction enzyme site and an MunI restriction enzyme site. In a eukaryotic cell, the U6 promoter is responsible for starting transcription of epegRNA, and the TTTTTTT terminator is responsible for terminating transcription. The vector can be conveniently inserted into a spacer sequence of epegRNA after being subjected to BbsI enzyme digestion, and then can be conveniently inserted into RTT and PBS sequences of the epegRNA after being subjected to LguI and Mva1269I double enzyme digestion. The universal skeleton vector is especially suitable for constructing eukaryotic expression vectors of different epegRNAs in batches, the cost of synthesizing DNA sequences in vitro is saved, the operation is simple, and the operation can be carried out by mastering a simple molecular cloning technology.