3 results about "Structural motif" patented technology

Heterocyclic compounds with a novel pyrazole thioamide-based NNSN structural motif, having highly effective zinc- or copper-activated toxicity against microbial infections at micromolar or nanomolar minimum inhibitory concentrations (MIC), and methods of making and using the same.

This invention belongs to the field of biotechnology and relates to a method for rapidly preparing three-dimensional self-assembled DNA crystals in a closed homogeneous system. The operation process of this invention is highly simplified, employing a "one-click annealing" mode. The reaction conditions are highly standardized, exhibiting excellent batch-to-batch reproducibility, and can stably obtain DNA crystals with regular morphology and uniform size, laying the foundation for subsequent large-scale preparation. More importantly, this strategy demonstrates good versatility and scalability, having been successfully validated on DNA structural motifs of different geometries and sizes (such as 2-turn and 4-turn stretched integral triangles). Its universality has been proven through two independent pathways: specific sequence design and chemical modification, indicating its potential to develop into a universal platform for DNA crystal construction.

The present disclosure discloses a programmatic design method for a topological protein, the method comprising the following steps: i) splitting an original structure of a protein-of-interest, and designing possible rewiring approaches according to a target topological structure; ii) evaluating connection approaches between structural motifs and determining priorities of the connection approaches in a subsequent design; iii) for each of the connection approaches, generating new virtual loop regions successively, exhausting all possible combinations of generation orders of the loop regions, creating corresponding spatial relationships, determining the formed chemical topological structures, calculating a formation probability of the target topological structure, and determining a length range of a newly-generated loop region; and iv) designing a length and sequence of the newly-generated loop region of the topological protein. The programmatic design method for topological proteins provided in the present disclosure provides a desirable platform for illustrating the structure-activity relationship of topological proteins and also provides a convenient method for developing functional topological proteins.