The invention discloses a fluorescent dark field
microscopy device and method based on
waveguide constraint. The fluorescent dark field
microscopy device comprises an illumination
laser light source, a beam expansion lens, a near-end reflector, a carrier
chip, a microobjective, a far-end reflector, a
light filter, a collection lens and a CCD (
Charge Coupled Device)
image sensor; the method comprises the following steps that: the carrier
chip which directly realizes the dark
field function is prepared; lasers emitted by the illumination
laser light source irradiate on a
polymer film in the carrier
chip through the near-end reflector after being subjected to beam expansion;
fluorescence light emitted from fluorescent molecules in the
light source forms a guiding mode in the carrier chip and is constrained at an interface of the
polymer film and air, and thus, the bottom of a sample nearby the carrier chip is lightened by a near field and scattered; then, light is collected by the microobjective, after the illumination lasers are filtered by the far-end reflector and the
light filter, the light is collected by the collection lens and forms an image at the CCD
image sensor. The device and the method are based on the constraint of a fluorescent
waveguide mode, the
microscopy structure is simplified, and the dark field microscopy function is realized.