The invention discloses a device and method for full-automatically sorting circulating
tumor cells on a micro-fluidic
chip. The device mainly comprises the PDMS micro-fluidic
chip and an
electromagnetism microvalve structure. The PDMS micro-fluidic
chip comprises
liquid storage holes (A, B, C, D, E and F), a detecting channel, a
main channel, a first focusing channel, a second focusing channel, a sample outlet channel, a target
cell collecting channel and an electromagnetic sorting channel. When the circulating
tumor cells pass through the detecting channel, produced
voltage signals are detected and sent to an NI collecting card and a
processing terminal, the
processing terminal sends out a
voltage signal through the NI collecting card so that an electromagnetic
relay can be switched off, the electromagnetic microvalve structure presses a PDMS layer below the electromagnetic microvalve structure, the PDMS layer deforms, a part of liquid is drained out of the
liquid storage hole E, and the circulating
tumor cells are pushed to flow to the target
cell collecting channel. According to the device, the embedded type electromagnetic microvalve structure is used for providing driving force for sorting, electroosmosis driving and electroosmosis focusing are adopted on samples, and the circulating tumor cells can be detected and sorted in a full automatic mode.