The invention provides a conditioning method used before sludge dewatering. The method includes the following implementation steps that 1, the temperature and pH of sludge are regulated to be within the proper range of a bio-enzyme; 2, the bio-enzyme is added into the sludge and mixed evenly, and a reaction is carried out for a period of time; 3, a certain amount of CTAB is added into the sludge, stirred and mixed, a reaction is carried out for a period of time, and then the mixture enters a dewatering unit. According to the method, the EPS component degrading and bound water releasing effects of the bio-enzyme and the hydrophobicity-hydrophilicity and charge neutrality of CTAB are combined, the advantages of the bio-enzyme and the advantages of CTAB are fully exerted, synergic effects are achieved, and the moisture content of sludge cakes is reduced to be 70% or lower after the sludge is dewatered. Besides, in the method, a conditioner is low in cost and wide in application range, all conditioning steps are clear and definite, operation is convenient, reduction is obvious, and the follow-up disposal cost of the sludge can be reduced.