6 results about "Screening assay" patented technology

The invention provides for a screening assay-using a selective proteolysis of recombinant full-length PIKfyve at an endogenous caspase 3 cleavage site to liberate the 34 kDa C -terminal kinase domain. Despite instability of the kinase domain to isolation, the liberated kinase domain is stable in the presence of the N-terminal PIKfyve protein fragment and can thus be treated with compounds of interest and analyzed by mass spectrometry, such as RapidFire-MS, with each injection taking under one minute. The assay was established to triage small molecules for covalent modification of PIKfyve. The assay was also established using cysteine-mutant proteins, where the reactive cysteine was replaced with an inert amino acid which when run through the same experimental protocol, was used to confirm adduct formation at the target cysteine.

A method for designing a cell-based screening assay experiment includes, at a graphical user interface, receiving a first input identifying a template plate definition and receiving a second input defining a first group of disease reagents and at least one destination concentration of the disease reagents. The method also includes receiving a third input defining a first group of treatment reagents, at least one destination concentration for the treatment reagents, a number of replicates of the treatment reagents, and a selection to associate the first group of treatment reagents with the first group of disease reagents. The method further includes generating, based at least partially on the inputs, a design for the cell-based screening assay experiment, including determining a number of test wells required for performing the experiment, and distributing the test wells over a one or more test plates that are instances of the template plate definition.

A method for screening and detecting biological particles includes the following steps: providing a biological particle screening and detection system, comprising a flow channel, an optical detection component, and a carrier disk, wherein the optical detection component provides a detection optical path that penetrates the flow channel, and the optical detection component is used to sense a target biological particle; providing a sample; diluting the sample to form a test solution; controlling the test solution to flow into the flow channel from an inlet end; and when the optical detection component senses the target biological particle in the test solution, the carrier disk is controlled to move to an outlet end of the flow channel, wherein the carrier disk contains a diluent, and a portion of the solution containing the target biological particle in the test solution is controlled to be discharged from the outlet end and then loaded onto the carrier disk to dilute the portion of the solution.

Antibodies that bind to SNAP-25 are provided. Botulinum toxin potency screening assays that utilize anti-SNAP-25 antibodies are also provided.

A method for screening a candidate agent for its ability to modulate RAGE activity, such RAGE activity being induced by an active coexisting GPCR, the method comprising the steps of contacting a RAGE polypeptide with a GPCR polypeptide in the presence of the candidate agent, wherein the GPCR polypeptide is constitutively active and / or activated by the addition of an agonist, partial agonist or allosteric modulator of the GPCR; and detecting whether the candidate agent is a modulator of RAGE ligand-independent RAGE activation by activated coexisting GPCR by detecting an effect indicative of modulation of RAGE activation by the presence of the candidate agent and / or by detecting RAGE-dependent signaling that is modulated by the presence of the candidate agent.