6results about How to "Improve inflammation" patented technology

The application discloses a method and application of knocking down ubiquitin-specific peptidase 7 protein, and belongs to the field of biological medicine. The method for knocking down the USP7 protein comprises: specifically inhibiting the expression of USP7 in endothelial cells through gene intervention, and the gene intervention means comprises shRNA, siRNA or recombinant virus carrier-mediated USP7 expression down-regulation. Further provided is a USP7 knockdown reagent for preparing a drug for treating endothelial cell inflammation or a cardiovascular disease, in particular, the USP7 knockdown reagent is a USP7 knockdown virus carrier containing a Cdh5 promoter; the cardiovascular disease comprises heart failure, myocardial infarction or ischemic cardiomyopathy. The application specifically knocks down the expression of USP7 in endothelial cells through gene intervention, significantly improves the migration, tube formation capacity and reduces the apoptosis of the endothelial cells, repairs endothelial dysfunction, the USP7 knockdown virus carrier is used for preparing a drug for treating a cardiovascular disease, and has important clinical value.

The invention relates to the technical field of medicines, and discloses a preparation method and application of an alcohol extract of root tuber of pilose asiabell. The preparation method comprises the following steps: crushing dried tuberous roots of cayenne pepper, sieving, weighing cayenne pepper powder, adding 95% ethanol, soaking and extracting at room temperature for 2 days, stirring once within 2 hours, once stirring for 30 minutes, performing suction filtration after soaking to obtain ethanol leaching liquor, soaking and extracting filter residues for 2 times, merging the ethanol leaching liquor, transferring into a rotary vacuum concentrator, and concentrating to obtain the cayenne pepper pure extract. Concentrating and drying to obtain a maozhou root tuber alcohol extract; experimental verification shows that the prepared pure extract of the sauced head has the effect of relieving the fatty liver, so that the pure extract of the sauced head can be used for preparing the medicine for relieving the fatty liver.

The invention provides a composite active dressing for neural restoration and a preparation method and application thereof, and relates to the technical field of medical materials. The composite active dressing comprises a transdermal inner layer and a small extracellular vesicle slow-release outer layer, the transdermal inner layer is of a membrane-shaped structure and comprises a composite active component and a hydrogel matrix material, and the composite active component is selected from one or more of an anti-inflammatory component, a microcirculation improving component, an antioxidant component and a neurotrophic component; the small extracellular vesicle sustained-release outer layer is of a three-dimensional network structure and comprises small extracellular vesicles and a hydrogel matrix material. According to the invention, stable loading and layered deep delivery and release of the small extracellular vesicles are realized, the inflammatory response and microcirculation state of an injured part are improved, proliferation migration and remyelination of Schwann cells can be promoted, and axon regeneration and effective repair of nerve injury can also be promoted.

This invention discloses a traditional Chinese medicine composition for treating ulcerative colitis, its preparation method, combined drugs, and applications. The traditional Chinese medicine composition for treating ulcerative colitis of this invention is made from the following raw materials in parts by weight: Astragalus membranaceus 13-26 parts, Lonicera japonica 10-20 parts, Sargentodoxa cuneata 10-20 parts, Prunus mume 8-16 parts, Agrimonia pilosa 8-16 parts, Allium macrostemon 6-12 parts, Platycodon grandiflorus 6-12 parts, Coptis chinensis 3-6 parts, and Panax notoginseng powder 1-3 parts. An animal model of ulcerative colitis was constructed using DSS induction, and the results showed that the traditional Chinese medicine composition of this invention has a certain therapeutic effect on ulcerative colitis. Clinical studies showed that the traditional Chinese medicine composition of this invention, used in combination with mesalazine enteric-coated tablets, can reduce the modified Mayo score and serum inflammatory markers in patients with active ulcerative colitis, improve colonic mucosal inflammation and gastrointestinal symptoms, and effectively treat active ulcerative colitis of the damp-heat type.

This invention provides a nanomaterial for relieving inflammation and pain in tendinopathy and its preparation method, relating to the field of biomedical technology. The nanomaterial comprises decellularized tendon DTM and hybrid membrane vesicles (MSM) of macrophages and nerve cells. The aforementioned nanomaterial for relieving inflammation and pain in tendinopathy includes decellularized tendon DTM and hybrid membrane vesicles (MSM) of macrophages and nerve cells. Combining these two components yields DTM-MSM, which regulates the proliferation and differentiation of tendon stem cells, modulates the local immune microenvironment, and relieves pain. It can improve inflammation and pain in tendinopathy and accelerate the repair of damaged tendons. In other words, DTM-MSM can not only alleviate the pathophysiological changes in tendinopathy but also rapidly improve the main clinical symptoms of tendinopathy, such as pain, swelling, and functional limitation, thus overcoming the current shortcomings of tendinopathy treatments, including slow onset of action, poor compliance, significant side effects, and unclear efficacy.

This invention discloses the application of *Lactobacillus mucosa* vesicles in the preparation of drugs for treating MAFLD, belonging to the field of microbial medicine. The *Lactobacillus mucosa* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.37136, and its 16S rDNA gene sequence is shown in SEQ ID NO: 1. The *Lactobacillus mucosa* strain was isolated from fecal samples of healthy adults. This strain exhibits significant antioxidant, anti-inflammatory, and lipid metabolism regulating activities in vitro. Its secreted EVs are non-cytotoxic and non-immunogenic in vivo, and animal experiments have verified its good in vivo safety and liver-targeted enrichment characteristics.