The invention discloses an
endothelial progenitor cell (EPC) culture method. The culture method comprises the following steps: inoculating human
peripheral blood mononuclear cells in a
cell culture
bottle coated by I-type
rat tail collagen, adding a DMEM culture medium in the
cell culture
bottle, putting the
cell culture
bottle in an
incubator with the temperature of 37 DEG C, the CO2 content of 5 percent and the
humidity of 95 percent for culturing; carrying out EPC
digestion passage; then carrying out flow cytometric analysis on EPC; carrying out EPC
immunofluorescence assay; carrying out a cell proliferative
assay; carrying out a single cell clonogenic
assay; carrying out FITC-UEA-1
extracellular adsorption and
endocytosis Dil-acLDL experimental measurement; and carrying out an in-vitro
tube formation experiment. The EPC culture method is simple, convenient and feasible. With the adoption of the EPC culture method, the cost can also be greatly lowered; the high-purity I-type
rat tail collagen is prepared by the EPC culture method and used for culturing EPC by
coating the
cell culture bottle, and the culture medium which is low in cost is also adopted, so that the EPC is successfully cultured from human
peripheral blood; through identification, the EPC cultured by the method disclosed by the invention has the phenotypic characteristics and the functions of the EPC cultured by the conventional method.