The invention discloses a composite microbial agent for detoxifying liver and a preparation method thereof, the composite microbial agent comprises a strain and an adsorption carrier; wherein the strain is prepared by using Streptococcus thermophilus, Lactobacillus casei and Bacillus megaterium. the strain comprises the following components by weight percentage: 30-40% of Streptococcus thermophilus, 25-35% of Lactobacillus casei and 25-35% of Bacillus megaterium, and the weight ratio of the total strain to the adsorption carrier is 1:0.9-1:1.1. The synergistic effect is generated by reasonablecombination of Streptococcus thermophilus, Lactobacillus casei and Bacillus megaterium. The beneficial bacteria can degrade the pesticide residues in fruits and vegetables, food additive residues, excessive fat intake, alcohol and other harmful substances, which can reduce the burden on the liver, and can help the digestion and absorption of nutrients in the food and supplementing various vitamins and amino acids, can significantly reduce serum transaminase, effectively alleviates the disease relapse of chronic hepatitis, and has good curative effect for chemical liver injury.