Preparation method of highland barley vinasse polypeptide oral liquid for activating ethanol dehydrogenase

The preparation of highland barley lees polypeptide oral liquid using ultrasound-assisted alkaline proteolysis and ultrafiltration technology solves the problem of low utilization rate of highland barley lees resources, improves the activation rate and antioxidant capacity of alcohol dehydrogenase, significantly reduces alcoholic liver damage, and achieves high-value utilization of resources and hangover relief and liver protection effects.

CN121942909APending Publication Date: 2026-05-01QINGHAI UNIVERSITY
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Patent Information

Application Number
CN202610032956.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-12
Publication Date
2026-05-01

AI Technical Summary

Technical Problem

In existing technologies, the utilization rate of highland barley lees resources is low, the protein extraction rate is not high, the activation activity of the prepared polypeptide alcohol dehydrogenase is unclear or low, and the mechanism of its alcohol detoxification and liver protection in vivo has not been fully resolved.

Method used

Highland barley lees powder was treated with an ultrasonic-assisted alkaline enzymatic hydrolysis method, and polypeptide components with a molecular weight of less than 3000 Da were retained by ultrafiltration technology. The polypeptides were then compounded with honey and sea buckthorn extract treated at low temperature to prepare a highland barley lees polypeptide oral liquid.

Benefits of technology

It significantly improved the activation rate of alcohol dehydrogenase and the DPPH free radical scavenging rate, reduced alcoholic liver damage, realized the high-value utilization of highland barley lees, and exerted the effect of detoxification and liver protection by regulating liver lipid metabolism and intestinal flora structure.

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Abstract

The invention relates to the technical field of functional food biology, and discloses a preparation method of highland barley vinasse polypeptide oral liquid for activating ethanol dehydrogenase, highland barley vinasse is used as a raw material, an ultrasonic-assisted alkaline protease enzymolysis technology is adopted, directional enzymolysis is carried out under an optimal process, and active polypeptide components with the molecular weight smaller than 3000Da are cut off through ultrafiltration, so that the highland barley vinasse polypeptide oral liquid is obtained. And then scientifically compounding with a honey extracting solution and a sea-buckthorn diluent. Through identification, the polypeptide component is rich in specific active peptide fragments such as LGAPF and the like. Animal experiments prove that the oral liquid can significantly improve the activity of ethanol dehydrogenase and acetaldehyde dehydrogenase in vivo and effectively reduce serum transaminase and liver lipid peroxidation level; the action mechanism relates to up-regulation of lipid metabolism gene expression and regulation of key intestinal flora abundance such as Muraculum and the like. According to the invention, high-valued utilization of the highland barley vinasse resource is realized, and the prepared product has remarkable comprehensive effects of dispelling the effects of alcohol, protecting liver and improving intestinal microecology.
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