32 results about "Auricularia" patented technology

The invention discloses an auricularia plicata strain and a cultivation and domestication method thereof, and particularly relates to an auricularia plicata strain W-24 (Jiangxi No.1) and a cultivation and domestication method thereof, the auricularia plicata strain W-24 is preserved in China General Microbiological Culture Collection Center on June 11, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The cultivation method comprises the following steps: firstly, activating auricularia plicata strains on a PDA (potato dextrose agar) comprehensive culture medium, inoculating the auricularia plicata strains into a solid culture medium, and culturing at 24-26 DEG C for 30-35 days to obtain solid stock seeds; the solid stock seeds are inoculated into cultivation bags, the inoculation amount is 10-15 g / bag, the cultivation bags are sealed, the cultivation bags are placed in a cultivation room to be cultivated in a dark place at the constant temperature of 24-26 DEG C, after cultivation is carried out for 80-85 days, hyphae grow all over the fungus bags, and fruiting management is carried out; the auricularia plicata strain W-24 provided by the invention has the characteristics of short cultivation period, high yield, strong stress resistance and the like, and has a wide market prospect.

The invention discloses an auricularia plicata strain rich in iron and crude fibers and a cultivation method and application thereof, and belongs to the field of edible fungus cultivation. A new Auricularia plicata strain is separated and purified from an Auricularia plicata fruiting body on an anise tree in Dariaotun, love village, Yujiang district, Baijiang city, Guangxi Zhuang Autonomous District, is named as' Guiyun No.5 ', is preserved in Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No: 64801. According to the method, a set of auricularia plicata cultivation method is constructed through condition optimization, large-scale cultivation and planting can be achieved through the cultivation method, the planted auricularia plicata is rich in iron and crude fibers, and the method has important significance on deep development of edible mushrooms and preparation of the edible mushrooms into food or medicine rich in iron and crude fibers.

The invention discloses an auricularia auricula strain and a rejuvenation culture medium and a cultivation method thereof, and belongs to the field of edible fungus cultivation. A new auricularia crispa strain is separated from miscellaneous trees at the top of Aoba in Nanpond village, Shixan town, eight-step area, Hexanzhou city, Guangxi Zhuang Autonomous Region, named 'Guiyun No.7', and the preservation number is GDMCC No.65145. The invention further discloses a preparation method of the auricularia crispa strain. The invention also discloses a rejuvenation culture medium which comprises the following components: 20 g / L of barks, 20 g / L of glucose, 2 g / L of yeast extract and 20 g / L of agar, and the barks are cyclobalanopsis barks or maple barks. Through the rejuvenation culture medium, the auricularia auricula strain can be rejuvenated, and strain degeneration is prevented. The crisp agaric strain can also be directly applied to cultivation and production, large-scale cultivation and production of crisp agaric are achieved, and the current situation that only a small-scale experimental research stage exists in crisp agaric cultivation at present is changed.

This invention relates to the field of edible fungi technology, and discloses a new strain of Auricularia heimuer and its applications. The Auricularia heimuer strain JH-Y6 of this invention was deposited at the China Center for Type Culture Collection (CCTCC) on November 20, 2023, with accession number CCTCC No: M 20232280. Auricularia heimuer JH-Y6 possesses high tyrosinase activity, high melanin production, and excellent agronomic traits, and can be used in the screening and breeding of Auricularia heimu, the extraction process of melanin from Auricularia heimu, and its cultivation. It can also be used in the deep processing of food or health products.

The invention discloses a black fungus AMY protein and a coding gene and application thereof, and belongs to the technical field of protein engineering. The invention provides a black fungus AMY protein as well as an encoding gene and application thereof in order to excavate a key enzyme gene of a starch sucrose metabolic pathway in black fungus, and discovers and verifies that the black fungus AMY protein and the encoding gene thereof play a key regulation and control role in inducing synthesis of black fungus alpha-amylase through transcriptome sequencing. A solid foundation is laid for deeply exploring the function of a key gene of a starch sucrose metabolic pathway.

The utility model provides an agaric fungus bag sterilizing and inoculating all-in-one machine which is characterized in that the agaric fungus bag sterilizing and inoculating all-in-one machine comprises an inoculating device, a cleaning device and a collecting device, the cleaning device comprises a conveying device and two connecting rod cleaning pieces, the conveying device is arranged in the inoculating device, and one ends of the two connecting rods are arranged at the output end of the conveying device; the conveying device is used for driving the connecting rod to move. The top of the cleaning piece is fixedly connected to one end of the connecting rod. According to the sterilization and inoculation all-in-one machine for the agaric fungus bags, compost falling during punching of the inoculation device is collected through the collection frame, meanwhile, the interior of the collection frame is cleaned in cooperation with the connecting rod and the cleaning piece in the process that the conveying device conveys the agaric fungus bags, and then collection is conducted through the collection box; and therefore, the scattered compost can be conveniently collected in the later period, waste is reduced, and meanwhile, the interior of the inoculation device is conveniently cleaned.

The invention relates to a high-temperature-resistant auricularia delicata strain Adel 27, which has a preservation number of CCTCC NO: M 2025114, is dense in bacterial colony, long in growth period, high-temperature-resistant, single-piece clustered in sporocarp, small in auricular base, light brown in auricular piece and soft in texture, and can be used for auricularia delicata cultivation and breeding. The cultivated auricularia plicata is high in nutrient substance content, can turn moisture for 3-4 times, can greatly improve the yield and reduce the cost, and is suitable for a factory large-scale production mode of one-time harvesting.

The invention discloses application of auricularia auricula monomer polysaccharide ME-2 in preparation of drugs for preventing and treating fatty liver diseases related to metabolic dysfunction. The research of the invention systematically proves that ME-2 has a remarkable intervention effect on the whole course of MASLD for the first time. The invention discloses a breakthrough function of ME-2 as a liver metabolism regulator for the first time: by directly correcting liver lipid metabolism disorder (such as regulating core genes such as Srebp-1c, ACC1, FASN and the like), liver fatty degeneration can be relieved in a dose-dependent manner in the whole course of MASLD, and the serum transaminase level and dyslipidemia can be reduced. Importantly, the curative effect of the traditional Chinese medicine is derived from intervention on a metabolic root source, and is totally different from a known anti-inflammatory application mechanism. Meanwhile, ME-2 has the remarkable advantages of being clear in structure, taking metabolic regulation as a core, and being safe and non-toxic, and a brand new drug candidate is provided for prevention and treatment of MASLD.

The invention relates to the technical field of biological medicines, and discloses a traditional Chinese medicine composition for treating rhinitis and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 1 to 8 parts of fructus xanthii, 3 to 8 parts of flos magnoliae, 5 to 20 parts of radix saposhnikoviae, 15 to 20 parts of herba menthae, 5 to 10 parts of radix scutellariae, 1 to 10 parts of periostracum cicadae, 1 to 5 parts of fructus arctii, 0.5 to 4 parts of trametes robiniophila mycoplasm, 0.01 to 0.1 part of zinc oxide nanoparticles, 12 to 20 parts of hydroxymethyl chitosan, 2 to 5 parts of poloxamer 407 and 1 to 3 parts of poloxamer 188. According to the traditional Chinese medicine composition for treating rhinitis and the preparation method, the trametes robiniophila mycoplasm and the zinc oxide nanoparticles cooperate with the fructus xanthii, the flos magnoliae, the radix saposhnikoviae, the herba menthae, the radix scutellariae, the periostracum cicada and the fructus arctii to jointly treat allergic rhinitis. The traditional Chinese medicine and western medicine combined method is adopted, inflammatory response is relieved, the immunologic function of a patient is enhanced, meanwhile, medicine use is reduced, and wide application prospects are achieved.

The invention discloses a functional auricularia auricula polysaccharide-milk compound and a preparation method and application thereof.Auricularia auricula polysaccharide is extracted in a free radical mediation and NADES high-penetration mode after auricularia auricula sporocarp is subjected to ultramicro pretreatment, the technological process is green and environmentally friendly, operation is easy and convenient to implement, the polysaccharide yield and activity are improved, and the functional auricularia auricula polysaccharide-milk compound is prepared. The polysaccharide dissolution rate can reach 57% or above; the biological activity of the compound disclosed by the invention is obviously improved, such as active oxygen and active nitrogen removal capability; the weight loss and DAI score increase caused by DSS-induced ulcerative colitis are relieved, the inflammatory factor level is reduced, and symptoms are effectively relieved. The compound provided by the invention is derived from a natural product, avoids serious toxic and side effects caused by chemical synthetic drugs or hormones, does not observe obvious toxicity under an experimental dose, is high in safety, can be widely applied to the fields of healthy foods, health care products, special medical foods and the like, and has a wide industrialization prospect.

The invention relates to the technical field of food science, and particularly discloses a preparation method and application of auricularia auricula polysaccharide-zein glycosylation gels, the method comprises the following steps: dissolving zein in an ethanol solution to prepare a zein solution with the mass volume concentration of 8%-12%; auricularia auricula polysaccharide is added into the zein solution, the addition amount of the auricularia auricula polysaccharide is 2%-6% of the mass of zein, and stirring and mixing are conducted; adjusting the pH value of the mixture obtained by stirring to 12 by using alkali liquor; heating the mixture of which the pH is adjusted at 70-90 DEG C to react for 30-50 minutes; a product obtained after heating reaction is rapidly cooled to the room temperature or below, standing is conducted at the temperature of 2-6 DEG C, composite gel is formed, glycosylation modification is conducted on the zein through the auricularia auricula polysaccharide, and the technical bottleneck that the zein is extremely low in solubility in a water environment and unstable in conformation due to high hydrophobicity is effectively overcome.

The invention belongs to the technical field of edible fungus cultivation. The invention relates to an auricularia polytricha strain, in particular to a Jiangxi auricularia polytricha strain and a breeding method thereof. The invention provides a novel Auricularia polytricha (Auricularia polytricha) strain 'Jiangxi' (Auricularia polytricha) and an efficient breeding technology system of the novel Auricularia polytricha (Auricularia polytricha) strain. The strain is stable in character and high in auricularia polytricha fruiting capacity, and systematic artificial cultivation of auricularia polytricha is achieved for the first time. According to the invention, a liquid culture medium is optimized, so that the mycelium pellet density and biomass of the strain are respectively increased by 62.65% and 55.06%, and elaeagnus pungens, pterocarya stenoptera, ligustrum lucidum ait and sophora japonica are screened as optimal matrixes through cut-log cultivation; a high-yield bag cultivation medium is developed aiming at the strain, the biological efficiency is 75-83%, and the total yield of the third-generation fresh ears is about 130g / bag. The method breaks through the bottleneck that auricularia polytricha depends on wild collection, provides germplasm and technical support for industrialization, and has good application value.

The application provides auricularia auricular syntheses related genes and application thereof, and the auricularia auricular syntheses related genes are AaHQT gene, AaHCT gene and AaC3H gene. The application adopts cultivation material to regulate the expression amount of the auricularia auricular syntheses related genes, so as to improve the production of chlorogenic acid of the fungus. The cultivation material is composed of wood chips, bran, bean powder, calcium carbonate, sucrose, superphosphate, potassium chloride and water. The application discloses the related genes involved in the synthesis of chlorogenic acid in auricularia auricular for the first time, and provides a theoretical basis for the application of the fungus in the production of chlorogenic acid. The application puts forward the application of the auricularia auricular syntheses related genes in the production of chlorogenic acid for the first time, the application adopts cultivation material to regulate the expression amount of the auricularia auricular syntheses related genes, and the operation is simple and easy to implement. The added substances of the cultivation material are safe and harmless, the price is low, the application is economical and practical, and large-scale production is facilitated.

The invention provides a preparation method and application of a cynanchum auriculatum composition with a lactagogue effect, raw material powder is obtained after cynanchum auriculatum, colla corii asini, longan, fructus lycii, pawpaw, radix puerariae, rhizoma dioscoreae and poria cocos are pretreated, then the raw materials are extracted by adopting an ultrasonic-assisted subcritical water technology, and the cynanchum auriculatum composition is obtained. The preparation method is easy to operate, low in energy consumption, safe, environmentally friendly, high in extraction efficiency of plant raw materials and very suitable for low-cost large-scale production, and an oral preparation prepared from the cynanchum auriculatum composition is good in lactagogue effect and high in stability.

1. The name of the design product: inflatable doll (auricularia genius). 2. The use of the design product: for display. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: front view. 5. The top view has been shown in the perspective view 1, the bottom view has been shown in the perspective view 2, and the top view and the bottom view are omitted.

The present application provides a kind of auricularia polysaccharide composition and its application, it is related to functional health product technical field.The auricularia polysaccharide composition includes by weight parts: black fungus polysaccharide 1-10 parts, blue fruit extract 2-15 parts, hawthorn extract 1-5 parts, lotus leaf extract 1-5 parts, mulberry leaf extract 1-5 parts, cassia extract 1-5 parts.The black fungus polysaccharide is prepared by the green method of enzyme hydrolysis-semi-bionic extraction combined with magnetic induction electric field-ultrafiltration membrane purification;The blue fruit extract and other plant extracts are prepared by alcohol extraction or water extraction method respectively.The composition of six components synergistic effect, can significantly regulate blood lipid and blood sugar level, can be used for preparing blood lipid-lowering and / or blood sugar-lowering functional products.The raw materials of the composition of the present application are all natural products of medicinal and edible origin, safe and non-toxic, with broad market application prospect.

The invention provides a process for preparing Tremella fuciformis polysaccharide by enzymolysis, and the specific steps of the process include pretreatment of Tremella fuciformis fruiting bodies, enzymolysis reaction of Tremella fuciformis fruiting bodies and the like. In the invention, multiple enzymes are used to carry out enzymolysis reaction together, and key conditions such as material-liquid ratio, enzymolysis time, and enzymolysis temperature in the enzymolysis reaction are controlled, so that the obtained Tremella fuciformis polysaccharide has high yield, high purity, and low protein content, and the total sugar content of Tremella fuciformis polysaccharide can reach more than 90%. The invention uses enzymolysis to prepare Tremella fuciformis polysaccharide, and the reaction conditions are mild, so that the use of a large amount of organic reagents is avoided, and the Tremella fuciformis polysaccharide is safe and environmentally friendly; the preparation process is simple, and the reaction process does not rely on equipment, and the production cost is low. Tremella fuciformis polysaccharide prepared by the enzymolysis method of the invention can be widely used in cosmetic products, and has excellent antioxidant and moisturizing effects.

The application discloses a kind of preparation auricularia auricular polysaccharide / casein emulsion gel and its preparation method and application.The preparation method of the application includes the following steps: 1) auricularia auricular polysaccharide aqueous solution is mixed with casein aqueous solution, to obtain protein-polysaccharide complex solution;2) water phase is mixed into oil phase, the water phase is the protein-polysaccharide complex solution, and the obtained mixture is ultrasonically homogenized, to obtain emulsion;3) gluconic acid-delta-lactone is added to the emulsion and mixed, incubated, to obtain auricularia auricular polysaccharide / casein emulsion gel.The auricularia auricular polysaccharide / casein emulsion gel disclosed in the application is applied to curcumin encapsulation.After adding auricularia auricular polysaccharide, the gel performance of casein emulsion gel is significantly improved;After adding auricularia auricular polysaccharide, the loading rate of curcumin loaded in casein emulsion gel is significantly increased.

The application discloses a kind of for cultivating auricularia polymeric mite smoke agent, including the following weight parts of substance: lufenuron 1~3 parts, spirodiclofen 2~6 parts, Sophora flavescens 10~15 parts, combustion improver 25~30 parts, smoke agent 5~10 parts, flame retardant 4~8 parts, adhesive 3~7 parts, moisture-proof agent 3~5 parts, fuel 15~20 parts, stabilizer 3~5 parts.The application discloses the application of the polymeric mite smoke agent in the application of auricularia cultivation in preventing and treating saprophytic mite harm.The application selects spirodiclofen for preventing and treating mite harm in the process of auricularia cultivation by pesticide formula screening, and spirodiclofen has the effects of preventing and treating each life stage of mite, long persistence period, wide mite spectrum and safety to auricularia;Sophora flavescens is used in fuel, and has the effects of killing insects and mites;by using smoke agent dosage form, effective component forms highly dispersed system, and pesticide can be uniformly distributed to ground, wall, top and around bag, and small particles can pass through sponge, cotton aperture and enter inside bag, and disappear fast, with low residual amount.

This invention discloses a strain of Auricularia auricula-judae HN-7 ( Auricularia This strain (species name) and its applications belong to the field of edible fungi technology. It was deposited at the China Center for Type Culture Collection (CGMCC) on November 14, 2025, with accession number CGMCC No. 42368. The HN-7 strain exhibits rapid mycelial growth, strong resistance to mold, small auricle bases, large leaves, numerous folds on the ventral side, and a grayish-yellow dorsal surface with prominent villi. The polysaccharide and amino acid contents in its fruiting bodies are 28.9% and 39.5% higher than the control strain 781, respectively, indicating higher nutritional value and yield. This strain has significant application value in the cultivation of Auricularia auricula-judae. It enriches the germplasm resources of Auricularia auricula-judae and helps improve the economic benefits of the Auricularia auricula-judae industry.

The utility model relates to the field of autumn auricularia planting, in particular to an autumn auricularia planting warm shed which comprises a warm shed body and main position frames, the main position frames are symmetrically installed in the warm shed body front and back, a top plate is installed on the upper end faces of the main position frames, an electric sliding block is installed at the bottom of the top plate, a water tank is installed at the bottom of the electric sliding block, and a water pump is installed at the bottom of the water tank. The bottom of the water pump is provided with a T-shaped pipe, the bottom of the outer side wall of the T-shaped pipe is provided with a plurality of connecting pipes, the bottom of each connecting pipe is provided with a spray head, and the outer side of each connecting pipe is provided with a first valve. The operation efficiency is improved, and the labor cost is effectively saved.

This invention provides a rice cake rich in black fungus polysaccharides and its preparation method. The steps are as follows: S1. Crushed rice is sieved and mixed with natural polysaccharide excipients to form a slurry, which is then dried to obtain rice starch aerogel microspheres; S2. The slurry is fermented with Bacillus subtilis, rinsed, and dried to obtain microbially modified aerogel microspheres; S3. A cellulose nanofiber dispersion is prepared; S4. The dispersion is mixed with refrigerated egg white, stirred, and whipped to obtain nanofiber-reinforced egg white foam; S5. The modified aerogel microspheres and black fungus polysaccharides are added to the rice slurry and mixed evenly; S6. The mixture is gently folded with the egg white foam, extruded, and baked and cooled to obtain the finished product. This invention forms a closed loop through the physical anti-sticking properties of the aerogel microcarrier and the bio-toughening effect of the dual microbial network, ensuring molding and structural stability. The nanofiber-reinforced foam structure and the porous structure of the aerogel work synergistically to promote rapid moisture evaporation and improve crispness, while simultaneously encapsulating the black fungus polysaccharides to prevent high-temperature denaturation, ultimately achieving comprehensive optimization of product performance.

The present application relates to the growth prediction and regulation field of Auricularia arida, and discloses an intelligent control method for the cultivation process of Auricularia arida, which comprises selecting suitable organic raw materials and configuring them into culture medium, inoculating the strain into the culture medium which has been sterilized, and placing the culture medium in a cultivation environment which can regulate humidity, temperature, carbon dioxide concentration and air flow after inoculation, so that the mycelium can expand and grow in the culture medium under suitable environment. The present application sets early observation points to obtain the coverage area and distribution of the mycelium on the culture medium, so that the mycelium expansion state in the cultivation process can be monitored in real time, and the cultivation efficiency and quality of Auricularia arida can be predicted according to the early expansion and growth of the mycelium. The problem of insufficient mycelium expansion can be found in the early stage, which provides a basis for subsequent remedial measures and reduces the risk of affecting the formation of fruit bodies and yield due to poor mycelium growth in the later stage.

The invention belongs to the technical field of microorganisms, and particularly provides a Sebacinales strain LS01 and application thereof in promoting germination of pleione bulbocodioides seeds to form seedlings. The LS01 strain disclosed by the invention is an auricularia cerasus species, and the preservation number of the LS01 strain is CCTCC (China Center for Type Culture Collection) NO: M 2024813. The invention further provides application of the Auricularia lasiocarpa LS01 in promoting germination of pleione bulbocodioides seeds to form seedlings. Compared with a traditional tissue culture mode, the strain has the advantages of being low in cost, high in field cultivation survival rate and the like, can be used for industrial development of large-scale pleione bulbocodioides seedling breeding, and lays an application foundation for wild-imitating cultivation, species regression and population reconstruction of pleione bulbocodioides.

The invention belongs to the technical field of bioengineering, and particularly relates to a method for fermenting cynanchum auriculatum with lactic acid bacteria and application of a product in a product for strengthening the spleen, benefiting the stomach and promoting digestion. The method specifically comprises the following steps: (1) pretreatment: adding cynanchum auriculatum powder into water, and performing high-temperature stewing to obtain a pretreatment solution; (2) enzymolysis: continuously adding water into the pretreatment solution, and then adding an enzyme preparation for enzymolysis to obtain an enzymolysis solution; (3) enzyme deactivation and fermentation: sterilizing the enzymatic hydrolysate, adding plant lactobacillus at the temperature of 25-30 DEG C, and fermenting for 1-3 hours to obtain fermentation liquor A; and (4) secondary fermentation: adding a compound strain into the fermentation liquor A, fermenting for 14-18 hours to obtain fermentation liquor, and carrying out solid-liquid separation on the fermentation liquor to obtain an upper layer which is fermentation clear liquor. The content of C21 steroid saponin in the fermentation product is high, and the fermentation product has the effects of strengthening the spleen, benefiting the stomach, promoting digestion and the like.

The application discloses an agricultural biological stimulant preparation and a preparation method and application thereof, and belongs to the field of agricultural biological stimulant preparation. The agricultural biological stimulant preparation comprises the following components (in percentage by weight): 5-50% of water extract of by-product of Auricularia auricular's fruiting body, 1-20% of thymol, 0.5-10% of Tween, 1-10% of ethyl acetate and 2-80% of water. The thymol in the agricultural biological stimulant preparation is sufficient in the market and easy to obtain. The water extract of the mycorrhiza and / or the fruiting body of Auricularia auricular is a resource recycling of by-product of agricultural products, and is safe to the environment. The agricultural biological stimulant preparation is suitable for green and organic agricultural product production, and has remarkable economic and social benefits and wide application prospect. The biological stimulant preparation is used in foliar spraying of green vegetables, so that the yield and quality of the green vegetables can be remarkably improved.

The invention discloses an auricularia auricula polysaccharide feed for feeding laying hens, the auricularia auricula polysaccharide feed comprises auricularia auricula polysaccharide (AAP) and a basal feed, the basal feed comprises the following components in parts by weight: 62.5 parts of corn, 22.0 parts of soybean meal, 8.0 parts of mountain flour, 1.5 parts of calcium hydrophosphate, 0.3 part of salt, 0.12 part of methionine, 0.08 part of lysine and 1.0 part of premix, the auricularia auricula polysaccharide feed is prepared by mixing the auricularia auricula polysaccharide (AAP) and the basal feed, the polysaccharide content of the auricularia auricula polysaccharide is greater than or equal to 82.3%, and the addition amount of the auricularia auricula polysaccharide (AAP) is 100-300mg / kg AAP. The invention discloses the dose effect relationship of the auricularia auricula polysaccharide on the laying hens in the later laying period, the breeding benefit is improved by improving the feed formula, and the achievement can be directly applied to the breeding practice of the laying hens and has important application and popularization values.

The invention relates to the technical field of polysaccharide extraction, in particular to a method for improving biological activity of auricularia auricula polysaccharide by modifying auricularia auricula polysaccharide through steam explosion. The black fungus polysaccharide is obtained through steam explosion pretreatment, low-temperature drying and smashing, degreasing with absolute ethyl alcohol, water extraction and concentration, alcohol precipitation, deproteinization and dialysis and freeze drying. The steam explosion pretreatment is carried out under the conditions that the steam explosion pressure is 0.3-1.5 MPa, the steam explosion pressure maintaining time is 20-100 s, and the material-liquid ratio is (1: 10)-(1: 50) g / mL. The method disclosed by the invention has the beneficial effects that on the basis of the traditional steps of hot water extraction, ethanol precipitation, Sevage method deproteinization, dialysis purification and the like, three key technical means of low-pressure long-time steam explosion, immediate low-temperature drying after explosion and preferential ethanol degreasing are creatively adopted; the yield, the purity, the in-vitro anti-oxidation activity and the in-vivo anti-aging activity of the auricularia auricula polysaccharide are remarkably improved.

The invention relates to a method for improving quality and efficiency of black fungus. Specifically, the invention provides a quality and efficiency improving method for black fungus, and the method comprises the following technical measures: a step of puncturing, a step of putting the black fungus down to the ground and a step of arranging fields, namely a step of first-time cultivation, a step of second-time thinning and a step of third-time cylinder drying. And the technical measures of preventing, watching and dragging in the ear growing management link are taken. According to the method, the problems that the black fungus cultivation pollution rate is high, the black fungus is prone to flowing, and the quality is poor can be solved, the yield of the black fungus is increased, and the excellent quality-improving and efficiency-improving effects on the black fungus are achieved.

The invention discloses application of a secondary metabolite of mycoplasm of trametes robiniophila in preparation of a medicine for resisting hepatocellular carcinoma. The secondary metabolite of mycoplasm of trametes robiniophila is tryptanthrin. According to the invention, tryptanthrin with potential anti-HCC activity is identified from secondary metabolites of Bayer's fungus, Tryp-atLip is prepared, the problem of poor water solubility of tryptanthrin is overcome, and a bionic nano-drug Tryp-atLip-M1 is further constructed. The Tryp (at) Lip and the Tryp (at) Lip-M1 NPs can be used for remarkably improving the blood circulation time and the tumor targeting property of the tryptanthrin. The Tryp (at) Lip inhibits the proliferation of the HCC by down-regulating an NAD < + > / beta-catenin / MYCN signal channel. The Tryp (at) Lip-M1 can significantly activate NK cells in a tumor microenvironment, increase the infiltration level of the NK cells, improve the killing ability of the NK cells to tumor cells, and generate a synergistic anti-cancer effect by remodeling a tumor immune microenvironment.