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Method for simultaneously producing Auricularia polysaccharides and melanin

A technology of fungus polysaccharides and melanin, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of improving fermentation efficiency, high economic benefits, and shortening preparation time

Active Publication Date: 2017-07-04
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fungus polysaccharides and melanin are biologically active substances with good application prospects. The simultaneous production of fungus polysaccharides and melanin by liquid fermentation can obtain these two substances at the same time, but there is no research on the simultaneous production of fungus polysaccharides and melanin by liquid fermentation. report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for simultaneously producing fungus polysaccharide and melanin, the specific steps are:

[0023] 1) Activation of the strain: the Auricularia auricula strain with the preservation number CGMCC No.9711 was placed on the slant medium for activation, the temperature was controlled at 25~35°C, the pH was 5.5~6.5, and the activation time was 50~ After 100 hours, when the mycelia covered the slant medium, the activation was completed. The activation of strains is to transform the black fungus strains that are in a preserved state and have low vitality into vigorous, reproductive and metabolic strains, paving the way for the next step of expanding the cultivation of strains;

[0024]2) Shake flask seed culture: insert the slanted surface of the test tube into the shake flask filled with liquid medium for expansion culture, the filling coefficient is 0.15~0.25, and the rotation speed of the rotation culture is 150~200r / min. The temperature is 24~30℃, the pH is 5.7~6.5...

Embodiment 2

[0032] A method for simultaneously producing fungus polysaccharide and melanin, the most preferred step is:

[0033] 1) Strain activation: The Auricularia auricula strain with the preservation number of CGMCC No.9711 was connected to the slant medium for activation, the temperature was controlled at 30°C, the pH was 6, the activation time was 80h, and the hyphae were allowed to grow. Full slant medium, activation ends. Strain activation is to transform the black fungus strains that are in a state of preservation and have low vigor into strains with vigorous vigor, strong reproductive ability and metabolic ability, and pave the way for the next step to expand the culture of strains;

[0034] 2) Shake flask seed culture: insert the slanted bacteria in the test tube into a shake flask filled with liquid medium for expanded culture, the filling coefficient is 0.22, the rotation speed of the rotation culture is 150r / min, and the culture temperature is 28°C. The pH was 6 and the in...

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Abstract

The invention provides a method for simultaneously producing Auricularia polysaccharides and melanin. The method concretely comprises the following steps: 1, strain activation; 2, shaking bottle seed culture; 3, fermenting culture; 4, separation and purification of Auricularia auricula melanin; and 5, separation and purification of the Auricularia polysaccharides. Auricularia auricular can simultaneously generate the Auricularia auricular melanin and the Auricularia polysaccharides, and the melanin and the Auricularia polysaccharides are directly extracted from a fermentation liquid; a fermentation medium can induce the Auricularia auricular to secrete the melanin and the Auricularia polysaccharides, so the secretion amount of the melanin and the Auricularia polysaccharides is increased, the secretion amount of substances independent of the excellent quality of the products is reduced, the product fermentation efficiency is increased, and the product preparation time is shortened; and the products obtained after separation and purification have the advantages of high purity, non-destroy of the structure, high activity and high extraction rate.

Description

technical field [0001] The invention belongs to the field of biological fermentation, and in particular relates to a method for simultaneously producing fungus polysaccharide and melanin. Background technique [0002] Black fungus (Auricularia auricula) is also known as fine fungus, Sichuan ear, cloud ear, alias black cabbage, wood moth, tree chicken, wood fungus, ear, light fungus, elm ear, mulberry ear, etc., because it grows sideways on trees , named for its resemblance to a human ear. It is a traditional medicinal and edible fungus in my country. It is rich in nutrients and has the functions of nourishing qi and strengthening the body, nourishing qi and blood, moistening the lungs, stopping bleeding, relieving pain, and laxative. It is an important traditional Chinese medicine resource in our country. [0003] Studies have shown that the main active ingredients in fungus are melanin, fungus polysaccharides, amino acids, trace elements and so on. Melanin has biological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P1/02C12R1/645
CPCC12P1/02C12P19/04C12N1/145C12R2001/645
Inventor 杨海龙周化斌杨欢东
Owner WENZHOU UNIVERSITY
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