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Culture method of black fungus

A technology of black fungus and culture medium, which can be used in botanical equipment and methods, fertilizer mixtures, gardening, etc., and can solve the problems of low yield of fungus and destruction of forest resources, etc.

Inactive Publication Date: 2008-07-30
顾英梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are two methods of artificial cultivation: one is to harvest 10-20-year-old broad-leaved trees for artificial wood section cultivation, and the other is to use bagged cultivation. Both of these methods have their shortcomings. The wood-section cultivation of fungus seriously damages forest resources. And although existing bagged cultivation has saved forest resources, because black fungus is strict to external conditions such as temperature, humidity, makes its fungus output low

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  • Culture method of black fungus

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Embodiment 1

[0030] A method for cultivating black fungus is completed by the following specific steps:

[0031] a. Primary bacteria inoculation and cultivation: Sterilize the prepared primary bacterial culture medium at a temperature of 100-120°C for 40 minutes at a constant temperature, then lower it to normal temperature; Put the fungus mother species into the medium, control the temperature between 24-26°C, and grow for 15-20 days, that is, the cultivation of the first-level bacteria is completed;

[0032] b. Secondary bacteria inoculation and cultivation: Sterilize the prepared secondary bacterial culture medium at a temperature of 100-120°C and keep the temperature constant for 8-10 hours. Stop heating and cool down naturally for 4 hours, and let it dry in the room. Cool to room temperature, insert the first-grade bacteria obtained in step a into the second-grade bacteria culture medium, grow in the culture room for 30-50 days, and control the humidity at 45-60%. The room needs to be...

Embodiment 2

[0052] A method for cultivating black fungus is completed by the following specific steps:

[0053] a. Primary bacteria inoculation and cultivation: put the prepared primary bacterial culture medium in a test tube and put it into a pressure cooker for sterilization. The sterilization temperature is 100-120°C, constant temperature for 40 minutes, and then lowered to room temperature ; Inoculation of first-level bacteria, put the purified fungus parent species into the culture medium of the test tube, control the temperature between 24-26°C, grow for 15-20 days, and complete the cultivation of the first-level bacteria;

[0054] b. Secondary bacteria inoculation and cultivation: put the prepared secondary bacterial culture medium into a bacteria bag or bottle, and place it in an atmospheric pressure sterilizer for sterilization at a temperature of 100-120°C. Keep the temperature constant for 8-10 hours, stop heating and cool down naturally for 4 hours, let the room cool down to r...

Embodiment 3

[0061] A kind of cultivation method of black fungus, its

[0062] The ingredients of the primary bacteria culture medium include: 1000 parts of wheat bran, 1000 parts of water, 200 parts of agar and 200 parts of glucose powder. Finally, filter the juice, then mix the juice with other ingredients in the formula, continue heating, and boil the agar;

[0063] The ingredients of the secondary bacteria culture medium include: 78 parts of broad-leaved sawdust, 20 parts of wheat bran, 1 part of white sugar, 1 part of gypsum and water according to their parts by weight. Prepared by adding 120% water to the dry weight and stirring evenly;

[0064] The ingredients of the three-stage bacteria culture medium include: 30 parts of corn cob powder, 50 parts of rice straw pulverization, 2 parts of bean cake powder, 1 part of gypsum, 0.7 part of lime and water. The ingredients are mixed, and then 150% of water is added according to the weight of the dry ingredients and stirred evenly.

[00...

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Abstract

A cultivating method for an Auricularia auricular belongs to the cultivating technology of edible fungi, which comprises the steps of inoculation and cultivation of the first grade fungus, inoculation and cultivation of the second grade fungus, inoculation and cultivation of the third grade fungus, accelerating germination and growing to ears. The culture medium of the strain is made of broadleaf sawdust, soybean powder, gypsum, straw powder, wheat bran, white sugar, gypsum, lime and food grains, etc. The Auricularia auricular cultivated with the method is of large ear, thick flesh, rich nutrition and good taste, which has good shapes after the sun drying, thus having a good market prospect. In addition, with easy access to raw materials, low cost, short startup time and no land occupation, the method can be used in urban and rural areas with various conditions, which is not only suitable for factory management, but also suitable for household production.

Description

technical field [0001] The invention belongs to the cultivation technology of edible fungus, in particular to a cultivation method of black fungus. Background technique [0002] A small part of the black fungus currently on the market is collected wild, and most of them are artificially cultivated. There are two methods of artificial cultivation: one is to harvest 10-20-year-old broad-leaved trees for artificial wood section cultivation, and the other is to use bagged cultivation. Both methods have their shortcomings. The wood-section cultivation of fungus seriously damages forest resources. And although existing bagged cultivation has saved forest resource, because black fungus is strict to external conditions such as temperature, humidity, makes its fungus output low. Contents of the invention [0003] The invention aims at overcoming the deficiencies of the prior art, and develops a method for cultivating black fungus. [0004] A kind of cultivating method of black fu...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
CPCY02P20/133
Inventor 顾英梅
Owner 顾英梅
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