Liquid submerged fermentation culture method for hericium erinaceus

The technology of a liquid submerged fermentation and cultivation method is applied in the cultivation field of edible fungi, which can solve the problems of low biological yield of mycelium, and achieve the effects of high yield, high biological yield and fast growth rate.

Inactive Publication Date: 2011-02-16
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to solve the problem of low mycelium biological yield in the production method of Hericium erinaceus, and provide a liquid submerged fermentation method for Hericium erinaceus

Method used

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  • Liquid submerged fermentation culture method for hericium erinaceus
  • Liquid submerged fermentation culture method for hericium erinaceus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening of Hericium erinaceus strain Zhongnong Hericium 1

[0027] With 32 Hericium erinaceus strains (3 from the Institute of Agricultural Resources and Agricultural Regional Planning, Chinese Academy of Agricultural Sciences; 4 from the Institute of Vegetable and Flowers, Chinese Academy of Agricultural Sciences; others from the Institute of Edible Fungi, Fujian Sanming Fungi Research Institute, Jilin Province, Jiangsu Province and other places (the specific location is unknown), the 32 Hericium ericaceus strains described are all wild species) as materials, in Difco PDA TM Culture medium (a standard medium, produced in Germany) at 25±0.1°C for 7 days, measure the colony diameter, mycelial growth rate=colony diameter / 7d, the results (see Table 1) show that the agricultural resources and agricultural The No. 18 strain collected by the Regional Planning Institute from Changbai Mountain in Northeast China has the fastest growth rate, indicating that it has re...

Embodiment 2

[0030] Identification of Agricultural Hericium 1 in Example 2 Hericium Hericium Strain

[0031]The No. 18 Hericium strain screened in Example 1, namely "Zhongnong Hericium 1", was identified. Its morphological characteristics are: white and dense hyphae, suitable for pH 4-5, and a temperature of 24-26°C Under conditions of growth, the mycelial growth rate on Difco PDA medium (a standard medium, produced in Germany) was 5.8mm / d at 25°C. "Zhongnong Hericium 1" is a fruiting variety with low medium temperature, high quality, high yield and strong stress resistance. The optimum temperature for the fruiting period is 10-22°C, and the optimum temperature is 17±1°C. The fruiting body is solitary, pure white; the diameter of the fruiting body is 10cm-30cm, medium-sized; the length of the stipe is 2-4cm, and the length of the thorn is 2-5cm.

[0032] ITS sequence sequencing

[0033] The total DNA of Zhongnong Hericium 1 was extracted by the CTAB method, and the total DNA was used as...

Embodiment 3

[0042] The submerged fermentation culture of Hericium erinaceus 1 mycelium in embodiment 3 Hericium erinaceus

[0043] Follow the steps below:

[0044] (1) The strain is activated under aseptic conditions, from Zhongnong Hericium 1 (preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on December 23, 2009, and the preservation number is: CGMCC3536) mother species test tube Take out mycelium pieces with a size of 2-4mm×2-4mm, quickly inoculate them on the slant medium, plug them with silica gel plugs, and culture them in an incubator at 25°C for 15 days to obtain the activated strain of Zhongnong Hericium 1. The slant culture medium described therein is prepared according to the following method: peel the potatoes, weigh 200 g, cut into small pieces of about 1 cm3, add water and boil until the potatoes are soft but not rotten, and filter with 4 layers of gauze. Take the filtrate, add 20g of glucose and 20g of agar, heat ...

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Abstract

The invention discloses a liquid submerged fermentation culture method for hericium erinaceus, and belongs to the field of culture methods for edible fungi. The culture method comprises the following steps of: activating hericium erinaceus strains on a slant culture medium, then performing primary strain culture on a liquid seed culture medium, performing secondary strain culture on a liquid fermentation culture medium, and finally performing amplified culture on the liquid fermentation culture medium to obtain hericium erinaceus mycelium. The invention also discloses hedgehog hydnum 1 in the hericium erinaceus strains, a seed culture medium and a fermentation culture medium for hericium erinaceus culture. The biological yield of the hericium erinaceus mycelium obtained by the culture method is high and can reach 12g / L which is over two times as much as the fermentation production level of the current factory; the hedgehog hydnum 1 in the hericium erinaceus strains has high growth speed which can reach 5.8 millimeters every day; and moreover, the liquid seed culture medium and the fermentation culture medium have good culture effect so that the hericium erinaceus mycelium has quick growth and high yield.

Description

Technical field: [0001] The invention belongs to a cultivation method of edible fungi, in particular to a liquid submerged fermentation cultivation method of Hericium erinaceus, and also relates to a medium for fermentation cultivation. Background technique: [0002] Hericium erinaceus is named for its physical shape resembling the head of a small monkey. Also known as Hericium erinaceus, Hericium erinaceus, Lemon bacteria, Tricholoma erinaceus, Hebala (Tibetan name), Shanfu mushroom (Japan) and Bear head seedling (Europe). (Lv Zuozhou, Edible Fungi Cultivation, Higher Education Press, 2006, P262). Hericium erinaceus is a rare edible fungus with edible and medicinal value. Hericium erinaceus polysaccharides can significantly inhibit the growth of stomach, esophagus, liver and skin cancer, (Mizuno, T. International Journal of Medicinal Mushrooms 1, 1999. p105-119.) It has three main active ingredients: β-D-glucan, which has anti-tumor effect; ergosterol, which is the precur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00A01G1/04
Inventor 张金霞黄晨阳陈强高巍
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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