Auricularia polysaccharide with function of reducing blood fat and preparation method and application thereof

A technology of fungus polysaccharides and fungus, applied in medical preparations containing active ingredients, functions of food ingredients, polysaccharide/gum-containing food ingredients, etc.

Inactive Publication Date: 2018-07-10
SAAS BIOTECH & NUCLEAR TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, it has not been seen that the fungus polysaccharides pro

Method used

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  • Auricularia polysaccharide with function of reducing blood fat and preparation method and application thereof
  • Auricularia polysaccharide with function of reducing blood fat and preparation method and application thereof
  • Auricularia polysaccharide with function of reducing blood fat and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 polysaccharide powder

[0028] (1) Pulverize the fungus fruit body and pass through a 100-mesh sieve to obtain the fungus powder;

[0029] (2) Mix the fungus powder obtained in step (1) with water at a mass ratio of 1:40, and stir evenly;

[0030] (3) adopt hot pressing method, leaching under the condition of 120 ℃, 0.12MPa in airtight container for 80 minutes, obtain extract;

[0031] (4) centrifuge, take the extract of fungus in the upper layer, remove protein by Sevage method, and obtain polysaccharide solution of fungus;

[0032] (5) Precipitate polysaccharide with 95% ethanol at a volume ratio of 1:20, let it stand overnight, centrifuge, take the precipitate and dry it to obtain polysaccharide powder.

Embodiment 2

[0033] Example 2 Preparation of irradiated fungus polysaccharide and investigation of irradiation conditions

[0034] The polysaccharide powder of Example 1 is configured into a polysaccharide solution of 5 g / ml with distilled water; respectively irradiated with Co60-γ rays of 10 kGy, 100 kGy, and 1000 kGy at a dose rate of 7 kGy / min, and the irradiated fungus polysaccharide solution is dried used later.

[0035] Add water to the irradiated fungus polysaccharide powder to prepare a fungus polysaccharide solution with a final concentration of 2mg / ml, stir at 50°C at a speed of 170rpm / min, and measure the time and viscosity for complete dissolution. The effect of irradiation dose on the change of dissolution time is shown in figure 1 .

[0036] The experimental results showed that the dissolution time and viscosity of polysaccharides from fungus could be significantly reduced by irradiation with a dose of 1000kGy. The solubility and viscosity are related to the structural pro...

Embodiment 3

[0037] Embodiment 3 establishment of hyperlipidemia animal model

[0038] After 7 days of adaptive feeding, 50 4-week-old C57 male mice were randomly divided into two groups according to body weight: the first group of 10 mice was the normal group, which was given basal diet; the second group of 40 mice was the high-fat group, which was given high-fat diet Feed (D12451, Beijing Huafukang Biotechnology Co., Ltd.) to create animal models of hyperlipidemia. Mice had free access to food and water. After 12 weeks, blood was collected from the canthus vein of the mice to measure serum total cholesterol, triglyceride, high-density lipoprotein and low-density lipoprotein. The test data was statistically processed by the independent sample t test of SPSS19.0 statistical software.

[0039] The results showed (see Table 1), the serum total cholesterol, triglyceride, and low-density lipoprotein contents of the hyperlipidemia group were significantly higher than those of the normal group...

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Abstract

The invention belongs to the field of fungus utilization, and particularly relates to auricularia polysaccharide with the function of reducing blood fat and a preparation method and application thereof. The auricularia polysaccharide is obtained through irradiation technical processing. The auricularia polysaccharide with the function of reducing blood fat achieves the technical purpose of lowering serum cholesterol and low-density lipoprotein. The preparation method of the auricularia polysaccharide prepared through irradiation comprises the steps of 1, performing smashing to obtain agaric powder; 2, mixing the agaric powder and water and performing stirring; 3, adopting a hot-press method, and performing digestion in a closed container to obtain an agaric extracting solution; 4, performing centrifuging to obtain the agaric extracting solution, sampling a supernate, adopting a Sevage method for removing protein, and then obtaining an auricularia polysaccharide solution; 5, adding ethanol into the auricularia polysaccharide solution for precipitation to obtain polysaccharide powder; 6, adding water into the polysaccharide powder, adopting Co60-gamma ray irradiation treatment, and performing drying to obtain auricularia polysaccharide. The auricularia polysaccharide has the advantages of lowering viscosity and improving the solubility, and it is accidentally discovered that theauricularia polysaccharide has the function of reducing blood fat.

Description

technical field [0001] The invention belongs to the field of fungus utilization, and in particular relates to a fungus polysaccharide with blood lipid-lowering function obtained through irradiation technology and its preparation method and application. Background technique [0002] Irradiation technology is the use of X, α, β, γ and other rays to irradiate substances to produce physical and chemical reactions. It is widely used in industry, agriculture, and medical treatment, such as food preservation and storage, crop breeding and flower variation, and sterilization of medical supplies. , polymer material modification, waste treatment, etc. Polysaccharides are an important part of all living organisms. Due to their special physical and chemical properties, polysaccharides are widely used in food, medicine, cleaning supplies, papermaking, printing and other fields. Polysaccharides generally have a large molecular weight, and years of research have shown that the molecular w...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P3/06A61K31/715A23L33/125
CPCA23V2002/00A61K31/715C08B37/0003C08B37/006A23V2200/3262A23V2250/51
Inventor 陈祖琴黄文丽熊川
Owner SAAS BIOTECH & NUCLEAR TECH RES INST
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