The invention discloses a negative pressure sterilization and disinfection device and method for a clean room, the negative pressure sterilization and disinfection device comprises a sterilization and disinfection box and a filter box, the sterilization and disinfection box is communicated with the filter box through an air conveying
pipe, the sterilization and disinfection box is provided with an air inlet, the filter box is provided with an air outlet, and the air outlet is provided with an induced draft fan; a humidifying mechanism and a
plasma mechanism are installed in the disinfection and sterilization box, a dehumidifying mechanism and a
filter element are installed in the filter box, and the humidifying mechanism, the
plasma mechanism, the dehumidifying mechanism and the
filter element are sequentially arranged in the air circulation direction. The sterilizing box and the filtering box are separated and connected through the air conveying
pipe, activated species generated by
ionization of the
plasma mechanism in the sterilizing box are dissolved into water drops generated by the humidifying mechanism to form water mist with the sterilizing function, the air conveying
pipe is sterilized and disinfected, the air conveying pipe is prevented from polluting the clean room, the sterilizing effect of the clean room is improved, and the service life of the clean room is prolonged. The sterilization period is prolonged, and the production efficiency is improved.