The invention relates to a method for extracting SOD (
superoxide dismutase) by utilizing high-temperature
protein cellulose removal
column chromatography, and belongs to the technical field of SOD extraction.The method comprises the following steps that S1, a part of
plasma is extracted and put into a
beaker, and a
copper sulfate solution is added into the
plasma; s2, adding water which is 2-3 times of the
plasma into a
beaker; s3, the
beaker is heated, and stirring is conducted during heating; s4, the heated solution is filtered, and blood residues are filtered out; s5, taking the filtrate with the volume of 500 to 1000 [mu] / ml; s6, concentrating the filtrate into a solution with the concentration of 10000 to 50000 u / ml by using an ultrafilter; s7, adding
ammonia water into the solution; s8, adjusting the pH (Potential of
Hydrogen) value to 4.8 to 5; s9, the solution is heated again, during heating, 1% of a protective agent is added into the solution, the protective agent is a 20% solution prepared by adding water into
sulfate, the temperature is increased to 70-80 DEG C, filtering is conducted with
filter paper, and filtrate is taken; and S10, adsorbing the SOD in the solution to the
cellulose column. The
sulfate solution is used as a protective agent, so that when the temperature is raised to 80 DEG C, a large amount of
protein can be removed, and SOD does not lose activity.