The invention relates to a recombinant HSV (
Herpes Simplex Virus)
amplicon vector and application thereof. By using two replication deficient adenoviruses respectively carrying Cre and an operative linked component loxP-HSVoriS-pac-transgenic expression box-loxP, a novel HSV
amplicon vector is recombined in a coinfection
cell. Being different from the traditional HSV
amplicon vector taking bacterial plasmids as a skeleton, the amplicon vector does not contain a
bacteria copying sequence (colEorigin) and a resistance
gene component and only contains oriS of HSV, a pac sequence and a transgenic expression box. The recombinant HSV amplicon vector disclosed by the invention is used for preparing a novel HSV amplicon vector, which does not contain the bacterial
gene component and is used for various types of transgenic researches and tumour
gene treatments, and preparing an adenovirus treatment preparation for specific anti-HSV
virus and related diseases. The HSV amplicon vector recombined by the replication deficient adenovirus of the preparation in cells replicates itself by using infected wild HSV
virus and competitively inhibits or permanently expresses the antiviral genes so as to inhibit replication of wild HSV
virus. Therefore, the recombinant HSV amplicon vector can be used for resisting
HSV infection and treating related diseases thereof.