Novel pure culture of Auricularia auricula and artificial cultivation method thereof
A technology of artificial cultivation and black fungus, applied in botany equipment and methods, horticulture, application, etc., can solve problems such as no research reports, and achieve obvious effects of lowering blood lipids, high economic benefits, and obvious effects
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Embodiment 1
[0032] The artificial cultivation method of black fungus new strain CGMCC NO.8708, comprises the following steps:
[0033] (1) Making the mother species: collect the isolated fungus black fungus MCC126001, aseptically connect the strain to the slope of the mother species culture medium, and place it in a 25°C incubator for constant temperature and dark cultivation until the mycelia cover the slope and then produce mother species,
[0034] (2) Making the original seed: Aseptically insert the production mother seed into the original seed bag, place it in a 25°C incubator for constant temperature and dark cultivation, and obtain the original seed after the mycelium is full of material;
[0035] (3) Making production species: insert the original species aseptically into the production seed bag, place it in a 25°C incubator for constant temperature and dark cultivation, and obtain the production species after the mycelium is full of material;
[0036] (4) Cultivation: The commonly...
Embodiment 2
[0046] Analysis of the active components of the new black fungus strain CGMCC NO.8708
[0047] In this embodiment, 5 different samples including the new bacterial strain of the present invention are used to analyze the active ingredients. The 5 samples are respectively as follows. The active ingredient analysis includes: protein content, total sugar content, calcium content, iron content , Zinc content, phosphorus content, potassium content, magnesium content.
[0048] The five samples are as follows:
[0049] Sample 1: Northeast autumn fungus (Yuanbao No. 2), the cultivation time is inoculated in May, and the ears are grown in July, and the cultivation location is Dongning County, Heilongjiang Province;
[0050] Sample 2: Northeast black fungus (Yuanbao No. 2), the cultivation time is inoculated in January, and the ears are grown in March, and the cultivation location is Dongning County, Heilongjiang Province;
[0051] Sample 3: Tibetan wild fungus No. 6, the cultivation ti...
Embodiment 3
[0064] Analysis of blood lipid-lowering function of a new black fungus strain MCC126001 (CGMCC NO.8708)
[0065] Black fungus is considered in traditional medicine as a kind of edible fungus with strong blood lipid-lowering effect. Therefore, in this embodiment, we choose blood lipid-lowering animal experiments to test the different effects between several black fungus varieties.
[0066] The experimental animal of this embodiment adopts healthy adult male SD rats (purchased from Animal Experiment Center of Guangdong Province), with a body weight of about 200 grams. After adaptive feeding for 1 week, 6 rats were given maintenance feed as a blank control group. Eighteen rats were given high-fat diet as the model group. After 2 weeks, blood was collected from all rats to determine the levels of serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) to detect the modeling situation . After ...
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