The invention relates to the technical field of boletus aereus culture and particularly relates to a boletus aereus culture medium, a preparation method therefor and an application of the boletus aereus culture medium. The boletus aereus culture medium provided by the invention is prepared from wood shavings, straw, red soil, cereal grains, rice bran, gypsum powder, a minor-element additive and ayeast extract, wherein the wood shavings is one or a mixture of at least two of Chinese chestnut wood, Cyclobalanopsis glauca wood, alder and Betula luminifera wood. The limitation that materials of existing boletus aereus culture media are restricted to growth cycle, regions or culture use is broken through, the region of culture is enlarged, and meanwhile, the raw materials are wide in source, large in quantity and low in cost; and the preparation method is simple, and the production cycle is short. By adopting the boletus aereus culture medium provided by the invention, the growth speed ofmycelia of boletus aereus can be accelerated, the boletus aereus culture time is shortened, meanwhile, the boletus aereus yield, average boletus aereus weight and boletus aereus quality are improved,thus, the productivity is greatly increased, the production cost is reduced, and the boletus aereus culture medium has remarkable economic benefit.