105 results about "Amino-Levulinic Acid" patented technology

The invention relates to the technical field of compound fertilizers, and discloses a boron-zinc-molybdenum synergistic compound fertilizer, which is prepared from the following raw materials in parts by weight: calcium nitrate, ammonium polyphosphate, potassium sulfate, magnesium sulfate, disodium octoborate tetrahydrate, sugar alcohol chelated zinc, sodium molybdate and a synergistic auxiliary agent, the feed additive is prepared from a feed additive, an activator, 5-alginic acid oligosaccharide, aminolevulinic acid, sugar alcohol chelated magnesium, an antagonist, a stress-resistant enhancer and a coating material. According to the boron-zinc-molybdenum synergistic interaction compound fertilizer and the preparation process thereof, the disodium octoborate tetrahydrate, the sugar alcohol chelated zinc and the sodium molybdate are compounded according to a specific proportion, antagonism is avoided through proportion limitation, the synergistic effect of the three elements is remarkably enhanced, and the problem that a single element is insufficient or excessive is solved; chelated elements such as sugar alcohol chelated zinc and sugar alcohol chelated magnesium are combined with an activating agent (humic acid chelated calcium and silica sol) to form a gel-state chelate, so that the trace elements can be prevented from being fixed by soil, and the transfer efficiency of the trace elements in crops is improved.

The invention discloses a special organic-inorganic compound fertilizer for improving the yield and quality of rice in a rice field, and belongs to the technical field of agricultural fertilizers. The compound fertilizer comprises a three-layer structure consisting of a porous core layer, a quick release coating layer and a biodegradable film, wherein the porous core layer is prepared from ammonium polyphosphate, potassium humate, nano charcoal modified chicken manure, mineral source potassium fulvate and sodium carboxymethyl cellulose-bentonite composite colloid; the quick-release coating layer is prepared from sulfur-resin double-layer coated urea, amino acid chelated zinc, alginic acid complex boron, silica sol, ammonium molybdate, 5-aminolevulinic acid and nano cerium oxide; the biodegradable film is prepared from a film forming solution and a bioactive microbial agent. Through the design of a three-layer structure and the combination of an organic-inorganic-microorganism synergistic technology, accurate matching of nutrient release and a rice fertilizer requirement rule is achieved, meanwhile, soil health and environmental protection are considered, and an innovative solution is provided for green high-yield rice planting.

The invention belongs to the technical field of fertilizers, and particularly discloses coated 5-aminolevulinic acid as well as a preparation method and application thereof. The preparation method of the coated 5-aminolevulinic acid comprises the following steps: (1) melting: melting urea phosphate to obtain a coating agent; and (2) mixing: uniformly mixing the coating agent and 5-aminolevulinic acid powder in a high-tower granulator, carrying out spray granulation, and cooling and curing to obtain the coated 5-aminolevulinic acid. The preparation method disclosed by the invention is simple in process, and the prepared coated 5-aminolevulinic acid is good in stability and can promote the growth of chilies.

The invention provides an anti-aging composition and application thereof, the composition at least contains pyrroloquinoline quinone disodium salt, ergothioneine and 5-aminolevulinic acid or salt thereof in physiologically acceptable media, the composition contains PQQ, ergothioneine and 5-aminolevulinic acid (5-ALA) or salt thereof, the three components are compounded to achieve a synergistic effect, and the anti-aging effect is achieved. The dyeing intensity of beta-galactosidase is reduced, and the anti-aging effect is achieved.

The present invention belongs to the field of plant growth regulators, and specifically relates to a fruit coloring composition, a fruit coloring functional fertilizer and their applications. The fruit coloring composition comprises 1-aminocyclopropane carboxylic acid and a protective auxiliary agent, and the mass ratio of 1-aminocyclopropane carboxylic acid to the protective auxiliary agent is 1:0.1 to 200; the protective auxiliary agent is selected from 5-aminolevulinic acid, diethyl aminoethyl hexanoate or choline chloride. The present invention first discloses the use of protective auxiliary agents such as 5-aminolevulinic acid, which can effectively improve the adverse effects when ACC is applied alone, such as high dosage for coloring, easy to cause negative phenomena such as plant defoliation, fruit cracking, fruit drop and quality decline. When the effective application dosage is only 0.05 to 2.5 ppm, excellent coloring effect can be achieved. At the same time, the safety of exogenous application of ACC is effectively improved, and the planting effect of fruit and vegetable crops can be improved in aspects such as preventing fruit drop, increasing fruit sweetness, reducing phytotoxicity, and relieving the growth inhibition of stems, leaves and fruits.

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

This invention discloses a lipid assembly loaded with 5-aminolevulinic acid and its preparation method. The lipid assembly is a hollow sphere with a core consisting of a hydrophilic phospholipid-chelating agent conjugate formed by chelation with a reactive phospholipid PEG reagent. The outer layer of the phospholipid-chelating agent conjugate is loaded with 5-aminolevulinic acid via hydrazone bonds, and then combined with a radionuclide as an internal light source excitation photosensitizer. The lipid assembly is loaded with 5-ALA via hydrazone bonds. Utilizing the acid-sensitive bond-breaking characteristics of hydrazone bonds and the prodrug properties of 5-ALA, combined with a radionuclide labeled on the surface of the lipid assembly as an internal light source excitation photosensitizer, this invention can overcome the limitations of external light penetration depth in traditional photodynamic therapy, achieving deep PDT treatment. Combined with chemotherapy drugs, it can minimize the toxic side effects on normal tissues during the combined use of radionuclides and photosensitizers in targeted therapy.

The objective is to provide 5-aminolevulinic acid or its salts and a method for producing the same, which have fewer impurities and residual solvents and suppressed discoloration compared to conventional methods. The objective is to provide 5-aminolevulinic acid or its salts, wherein, based on peak area obtained by HPLC analysis, the ratio of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0007 or less, and the sum of the ratios of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0016 or less.

The invention discloses a saccharomyces cerevisiae engineering bacterium for producing heme-binding protein as well as a construction method and application of the saccharomyces cerevisiae engineering bacterium. The construction method comprises the following steps: (1) by taking Saccharomyces cerevisiae CEN.PK2-1C as an original strain, optimizing a metabolic pathway, enhancing the supply of cytoplasm alpha-ketoglutaric acid (alpha-KG) and succinyl coenzyme A, and realizing the efficient accumulation of 5-aminolevulinic acid (ALA); (2) regulating and controlling the heme synthesis gene cluster in a time-sharing manner, and cooperatively expressing heme binding protein, so as to improve the assembly efficiency of heme (Heme); and (3) a secretion system is optimized, the secretion bottleneck of eukaryotic protein is broken through, and transmembrane transport and extracellular accumulation of the heme-binding protein are realized. Through the transformation of the combination strategy, an engineering strain for efficiently producing the heme-binding protein is obtained, and the yield of the bean hemoglobin in the saccharomyces cerevisiae is remarkably improved. Under the fermentation condition of a 5L fermentation tank, the secretory expression quantity of the bean hemoglobin reaches 286.6 mg / L.

The invention relates to the technical field of chemical substance preparation, and particularly discloses preparation equipment and a preparation method of 5-aminolevulinate, the preparation equipment comprises a preparation table, and further comprises a feeding unit arranged on the preparation table; the screening unit is arranged below the feeding unit; through the arrangement of the feeding bin, crushing of raw materials can be achieved, meanwhile, the raw materials can be screened during crushing, secondary screening is conducted through the screening bin below after screening, meanwhile, through the arrangement of the first driving unit and the moving unit, the screening bin can be driven to move while feeding is conducted, and in the back-and-forth moving process, the screening efficiency is improved. The feeding bin and the screening bin are impacted by the moving strips to generate vibration, so that the screening efficiency of the feeding bin and the screening bin is improved, raw materials clamped on the screening holes and the screening plate can be vibrated off through impact, the raw materials can be cleaned without shutdown, the caked raw materials can be crushed in the impact process, and the screening efficiency is improved. And therefore, subsequent screening work is facilitated.

The invention provides a method for detecting chloroethane in hexyl aminolevulinate hydrochloride and application, the method comprises the following steps: preparing a reference solution and a test solution, the reference solution is a standard chloroethane solution, and the test solution is a hexyl aminolevulinate hydrochloride solution containing chloroethane; respectively injecting the reference solution and the test solution into a GC-MS (Gas Chromatography-Mass Spectrometer) by adopting a headspace sampling mode, and recording chromatograms; calculating the content of chloroethane in the test sample according to a peak area by an external standard method; the used gas chromatographic column is DB-FFAP, and the adopted gas chromatographic determination conditions are as follows: the flow of the chromatographic column is 1-10ml / min; the initial temperature of the column temperature is 25-50 DEG C, the initial temperature is maintained for 1-10 minutes, and the initial temperature is increased to 150-250 DEG C at the rate of 10-50 DEG C / min and maintained for 1-10 minutes; the temperature of a sample inlet is 150-30 DEG C; the sample size is 0.5 to 5 ml; the mass spectrum conditions are as follows: the operation time is 5-20 minutes; and the mass-to-charge ratio of chloroethane is 64 and 66. The method can be used for accurately and quickly detecting the content of chloroethane in hexyl aminolevulinate hydrochloride.

This invention belongs to the field of agricultural herbicides and provides a photosynthetic inhibitor herbicidal composition comprising 5-aminolevulinic acid, malondialdehyde, and a herbicidal active ingredient. The herbicidal active ingredient is selected from one or a combination of several herbicides selected from acetolactate synthase inhibitors, photosynthetic system II inhibitors, or protoporphyrinogen oxidase (PPO) inhibitors. This invention utilizes the synergistic effect of 5-aminolevulinic acid, malondialdehyde, and a specific type of herbicidal active ingredient to effectively enhance the efficacy of the herbicidal active ingredient, enabling rapid and thorough weed control.

The invention relates to the technical field of plant cultivation and nutrition, and discloses a treatment method for enhancing coloring and sugar increasing of citrus fruits based on 5-aminolevulinic acid. And phosphite serving as a carbon immobilization catalyst and polyhydric alcohol serving as a product transfer accelerant are creatively compounded. The preparation method comprises the key step of accurately regulating and controlling the pH value of the system so as to ensure the stability and efficient absorption of the preparation. According to the application method, foliage spraying is carried out in specific time windows of the young fruit stage and the expansion stage of citrus, so that the effect of the preparation is matched with the key physiological rhythm of fruit development. Through the synergistic effect of the composition and the method, the photosynthetic whole chain of light harvesting-carbon sequestration-transportation is systematically optimized, the soluble solid content and the sucrose content of the citrus fruits can be remarkably increased, the color turning of the peel is effectively promoted, and the method is safe, stable and outstanding in effect.

The invention provides a separation microneedle of a loaded lipidosome nanoreactor as well as a preparation method and application of the separation microneedle, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: by taking phospholipid, cholesterol, tetramethoxysilane and 3-aminopropyltriethoxysilane as raw materials, entrapping cis-platinum, 5-aminolevulinic acid and catalase to prepare a liposome nano-reactor; polyvinylpyrrolidone, citric acid, sodium bicarbonate and regenerated silk cellulose are used as raw materials, a liposome nano reactor is dispersed in a microneedle body through a pouring method, and the microneedle capable of being rapidly separated is successfully prepared. Experimental research proves that the separation microneedle has good biocompatibility and mechanical properties, can effectively break through cuticle and realizes efficient transdermal delivery of a liposome nanoreactor. The liposome nanoreactor can be locally planted in deep tumor tissues, continuously supplies oxygen through enzymatic reaction, effectively improves the difficulty of insufficient oxygen supply in tumor photodynamic therapy, and has obvious advantages and wide prospects in the aspects of skin tumor photodynamic therapy application and the like.

The object of the invention is to provide a pharmaceutical composition for enhancing the antitumor effect by an immune checkpoint inhibitor. Provided is a pharmaceutical composition for enhancing the antitumor effect by an immune checkpoint inhibitor comprising 5-aminolevulinic acids (ALAs) as the active ingredient.

The present application relates to the field of metabolic engineering and microbial fermentation, and in particular to a recombinant shewanella producing 5-amino levulinic acid and its application. The present application discloses a new 5-amino levulinic acid producing strain, which is obtained by modularly modifying shewanella by synergistically expressing 5-amino levulinic acid production module genes (hemA, hemL and gltX) derived from shewanella, antioxidant module genes (katE, sodB) derived from escherichia coli, and introducing inhibition modules (the inhibition genes are hemB2 and sucA), so that the shewanella can efficiently produce 5-amino levulinic acid, and the yield of 5-amino levulinic acid can be increased by 134.0 times compared with the original strain.

The invention provides a cleaning solution for a semiconductor chip after chemical mechanical polishing, and a preparation method and application thereof, the cleaning solution for the semiconductor chip after chemical mechanical polishing comprises the following components by mass: 5-20 parts of a strong base; 5-30 parts of a weak base; 0.5-3 parts of a cellulose derivative; 0.5 to 2 parts of a chitin derivative; and 70-95 parts of ultrapure water. The cellulose derivative is one or more of 2, 5-furandicarboxylic acid, levulinic acid and 5-aminolevulinic acid. The chitin derivative is one or more of chitosan oligosaccharide, carboxymethyl chitosan, N-acetyl glucose, glucosamine and carboxymethyl glucosamine. According to the cleaning solution for the semiconductor chip after chemical mechanical polishing, CuO generated on the surface of copper after CMP can be effectively removed, a monomolecular adsorption layer is generated on the surface of Cu, and reoxidation of the surface of Cu is effectively inhibited within waiting time, so that Vbd is prevented from being reduced, and the reliability of a gate oxide layer of an MOS (Metal Oxide Semiconductor) device is improved.

The utility model discloses equipment for rapidly separating 5-aminolevulinic acid, and relates to the technical field of rapid separation of 5-aminolevulinic acid, the equipment for rapidly separating 5-aminolevulinic acid comprises a column body, the top end of the column body is provided with a top head seat, the upper end of the top head seat is provided with a feed inlet, and the lower end of the top head seat is provided with a discharge outlet. A bottom end base is arranged at the bottom end of the column body, a discharging port is formed in the lower end of the bottom end base, supporting legs are welded to the lower surface of the bottom end base, water inlet pipes are arranged on the two sides, close to the top base, of the column body, a water distribution assembly is arranged on one sides of the water inlet pipes, and a material distribution assembly is arranged at the upper end of the water distribution assembly. According to the scheme, the problems that most of existing separation columns for rapid separation of 5-aminolevulinic acid are subjected to single-barrel discharging and water discharging, are located in the centers of the columns and are subjected to single-point discharging and water discharging, the situation that more water is discharged in the middle and less water is discharged on the periphery is caused, and therefore distribution uniformity is caused, and the separation uniformity and efficiency are affected are solved.

The invention discloses an agricultural plant growth regulator composition and application thereof, the effective components of the agricultural plant growth regulator composition comprise allantoin and 5-aminolevulinic acid, the mass ratio of the allantoin to the 5-aminolevulinic acid is 1: 80-80: 1, and the mass ratio of the allantoin to the 5-aminolevulinic acid is preferably 1: 3-3: 1. The plant growth regulator composition provided by the invention has an excellent synergistic effect, the two components are matched, the growth and development of root systems are enhanced, the photosynthesis is promoted, the fruit setting rate and the fruit weight are increased, meanwhile, the mature period is shortened, fruits appear on the market as soon as possible, higher price can be obtained, higher benefits can be obtained with lower cost, and the plant growth regulator composition is safe to crops.

The invention relates to a high-content 5-aminolevulinic acid sustained-release pellet, which comprises the following components: 5-aminolevulinic acid phosphate; microcrystalline cellulose (MCC PH-102); hydroxypropyl cellulose (HPC-SL); 2-5% of pregelatinized starch; sodium carboxymethyl starch (CMS-Na); sucrose; the drug loading capacity is 60 + / -2%, and the bulk density is 0.65-0.75 g / cm < 3 >.

The application discloses a 5-aminoacetylpropionic acid-producing genetically recombined bacillus velezensis and application thereof, relates to the technical field of biotechnology and microbial metabolic engineering. The bacillus velezensis is obtained by integrating a hemA gene of Bradyrhizobium japonicum into the genome of bacillus velezensis R9, and the bacillus velezensis is obtained. The bacillus velezensis R9 has been preserved in the China Center for Type Culture Collection on July 5, 2023, and the preservation number is CCTCC NO: M 20231199. The strain can ferment to produce 17 mg / L of 5-aminoacetylpropionic acid in a 5-aminoacetylpropionic acid detection culture medium, and the concentration is more than 3 times higher than that of the bacillus velezensis R9, so the strain is an ideal engineering strain for producing 5-aminoacetylpropionic acid.

The present invention provides a composition comprising 5-aminolevulinic acid or a salt or ester thereof, comprising: (a) an active ingredient selected from 5-aminolevulinic acid or a salt or ester thereof; and (b) an aqueous carrier comprising an analgesic. The 5-aminolevulinic acid or the salt or ester thereof can be used for photodynamic therapy analgesia for more than 20 minutes, so that the clinical treatment cycle can be covered, the pain feeling after photodynamic therapy can be obviously relieved, the photodynamic therapy receiving time of a patient is prolonged, and the medication compliance of the patient is improved. Moreover, the composition provided by the invention can also improve the condition of tissue edema after photodynamic therapy, improve the skin smoothness after photodynamic therapy and shorten the pigmentation time, and meanwhile, compared with a marketed preparation, the composition has a better effect in the aspect of photodynamic therapy of inflammatory diseases (such as psoriasis or contact dermatitis) or acne and the like, and has a good application prospect. The acceptability of the patient to the photodynamic therapy is further improved; and the application scene of the photodynamic therapy is widened.

The invention relates to mRNA therapy for the treatment of Acute Intermittent Porphyria (AIP). mRNAs for use in the invention, when administered in vivo, encode human porphobilinogen deaminase (PBGD), isoforms thereof, functional fragments thereof, and fusion proteins comprising PBGD. mRNAs of the invention are preferably encapsulated in lipid nanoparticles (LNPs) to affect efficient delivery to cells and / or tissues in subjects, when administered thereto. mRNA therapies of the invention increase and / or restore deficient levels of PBGD expression and / or activity in subjects. mRNA therapies of the invention further decrease levels of toxic metabolites associated with deficient PBGD activity in subjects, namely porphobilinogen and aminolevulinate (PBG and ALA).

The embodiment of the invention relates to the technical field of medical equipment, and provides a skin lesion treatment light source determination method based on a Wood's lamp and a related device, and the method comprises the following steps: smearing 5-aminolevulinic acid on a target skin area; acquiring Wood lamp irradiation image data of the target skin area; inputting the Wood lamp irradiation image data into a pre-trained lesion degree identification model to obtain a lesion identification result; according to the lesion recognition result, determining lesion skin division area data; light source output doses of different infection zones are determined according to the lesion skin division zone data, the boundary ranges of an uninfected zone, a mild infection zone and a severe infection zone can be distinguished according to wood lamp irradiation image data of a target skin zone, and a light dose output strategy is dynamically matched according to the fluorescence intensity gradient of the zones, so that the target skin area can be identified. And the sterilization efficiency of a severe infection area is improved while excessive irradiation of normal tissues is avoided.

The invention belongs to the technical field of bioengineering and application, and particularly relates to application of a transcription factor ArnR in promoting biosynthesis of 5-aminolevulinic acid. The amino acid sequence of the transcription factor ArnR is as shown in SEQ ID NO. 1. The biological synthesis efficiency of the 5-ALA is improved by up-regulating the expression level of the transcription factor ArnR, so that the large-scale application of synthesizing the 5-ALA by a biological method is promoted.

A method of treating non-melanoma skin cancer or a precancerous skin condition in a patient is provided. The method includes applying to a lesion on the patient a topical composition including 5-aminolevulinic acid, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable excipient; incubating the topical composition on the lesion; and illuminating the lesion with a blue light source.

The invention discloses a herbicide composition containing 5-aminolevulinic acid, which consists of 5-aminolevulinic acid and an active component A. The weight ratio of the 5-aminolevulinic acid to the active component A is (20-80): (0.1-10). The composition significantly enhances the weeding effect through the synergistic effect of different action mechanisms, and has the characteristics of high efficiency, rapidness, safety, environmental protection and the like.

The present invention relates to the field of herbicides and specifically relates to an ACCase inhibitor herbicide composition containing 5-aminolevulinic acid. The composition comprises, by weight, 1 to 95 parts of an ACCase inhibitor herbicide, 0.1 to 30 parts of 5-aminolevulinic acid, 0.1 to 30 parts of a respiratory inhibitor, and 5 to 95 parts of an adjuvant. The herbicide composition can improve the herbicidal efficacy of ACCase inhibitor herbicides and their performance under high temperature and drought conditions, while reducing the dosage of the ACCase inhibitor herbicide and slowing the emergence of resistant weeds.

The invention provides a biological stimulant for regulating and controlling micro-ecological activity of acid soil rhizosphere as well as a preparation method and application of the biological stimulant, and particularly provides the biological stimulant which comprises high fructose corn syrup, tea polyphenol and 5-aminolevulinic acid. The biostimulant further comprises an amino acid and an oligosaccharide. The biological stimulant provided by the invention can improve the pH of rhizosphere soil, reduce exchangeable aluminum ions, improve soil respiration and mycorrhizal spore density, and realize yield increase and efficiency increase.

To provide a motility improver for male sperms.SOLUTION: The male sperm motility enhancing agent of the present invention contains nicotinamide mononucleotide (NMN), coenzyme Q10, and 5-aminolevulinic acid (5-ALA) as essential components. The agent for improving sperm motility can be used for infertility treatment of an adult male with reduced sperm motility and / or reduced sperm concentration, or for pregnancy activity (fertility) of an adult male. Another application is in the breeding of domesticated animals.SELECTED DRAWING: Figure 2