10 results about "Amino-Levulinic Acid" patented technology

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

This invention belongs to the field of agricultural herbicides and provides a photosynthetic inhibitor herbicidal composition comprising 5-aminolevulinic acid, malondialdehyde, and a herbicidal active ingredient. The herbicidal active ingredient is selected from one or a combination of several herbicides selected from acetolactate synthase inhibitors, photosynthetic system II inhibitors, or protoporphyrinogen oxidase (PPO) inhibitors. This invention utilizes the synergistic effect of 5-aminolevulinic acid, malondialdehyde, and a specific type of herbicidal active ingredient to effectively enhance the efficacy of the herbicidal active ingredient, enabling rapid and thorough weed control.

This application relates to the field of aquatic product logistics technology, and more particularly to an additive for improving the survival rate of yellow catfish during long-distance transportation and its preparation method. An additive for improving the survival rate of yellow catfish during long-distance transportation comprises the following raw materials: 0.2-0.5 parts of microbial preparation, 2-3 parts of glucose, 0.5-1 parts of compound vitamins, 3-5 parts of pH adjuster, 0.2-0.4 parts of 5-aminolevulinic acid, 0.8-1.2 parts of traditional Chinese medicine extract, and 91-95 parts of deionized water. The additive of this application, through the synergistic effect of microbial preparation, 5-aminolevulinic acid, and traditional Chinese medicine extract, improves hypoxia, improves water quality, enhances the immunity and stress resistance of yellow catfish, significantly increases the survival rate of yellow catfish during long-distance transportation, effectively solves the problem of low survival rate of yellow catfish during long-distance transportation, and can be widely used in the logistics and distribution of yellow catfish.

The present application relates to a kind of microdroplet photodynamic therapy compositions for treating skin and mucous membrane squamous cell carcinoma and its preparation method, belong to medical technical field.The microdroplet photodynamic therapy compositions for treating skin and mucous membrane squamous cell carcinoma, including photosensitizer precursor, anesthetic and solvent, the mass final concentration of the photosensitizer precursor is 15-25 %, the mass final concentration of the anesthetic is 4-6 %, and the photosensitizer precursor is hydrochloric acid aminolevulinic acid.The microdroplet photodynamic therapy compositions for treating skin and mucous membrane squamous cell carcinoma prepared by the present application adopts the mode of microdroplet injection, solves the three big problems of drug delivery, function preservation and pain control commonly faced in the treatment of different sensitive parts (face, hand, genital organ, tongue), enhances the practicability of the microdroplet photodynamic therapy compositions for treating skin and mucous membrane squamous cell carcinoma of the present application.

This invention provides an in-situ gel delivery carrier for 5-aminolevulinic acid and its preparation method, belonging to the field of biomedical technology. The in-situ gel delivery carrier comprises TAT ​​peptide and *Rhizopus oryzae* lipase; the mass-to-volume ratio of TAT peptide to *Rhizopus oryzae* lipase is 0.03-0.07 g: 0.005-0.015 g. This in-situ gel delivery carrier combines a dual mechanism of "physical thermosensitive phase transition" and "biological thermosensitive enzyme activity" to achieve long-term, targeted adhesion to lesion sites. Through the nonlinear synergistic effect of *Rhizopus oryzae* lipase and TAT peptide, it precisely degrades lipid barriers and efficiently delivers drugs, thereby significantly reducing the drug concentration while exponentially increasing the conversion efficiency of protoporphyrin IX (PpIX) in target cells. The preparation method is simple to operate.

This invention provides a 5-aminolevulinic acid producing strain, its construction method, and its application. Based on the existing strain JAL9, it involves knocking out… pflB、ldhA、poxB、adhE、ackA、dppA Genes, upregulated eamA、grxA、sodB The transcriptional level of the katE gene was downregulated. pfkB Gene transcription level, heterologous expression hemA ST Genes and xfp The gene, along with a high-copy plasmid PETC4, expresses genes from... Rhodobacter capsulatus of hemA RC Genes, ultimately through hemB Introducing an ASV degradation tag at the gene terminus enhances the degradation of HemB protein, effectively improving the synthesis efficiency of 5-aminolevulinic acid. This method has good industrial application value when applied to the fermentation production of 5-aminolevulinic acid.

This invention discloses an engineered *Yarrowia lipolytica* strain capable of methanol assimilation, its construction method, and its applications. By inserting AOX, CTA, DAK, and DAS genes into the genome of *Yarrowia lipolytica* strain BZ, a methanol assimilation pathway was constructed within the *Yarrowia lipolytica* metabolic network. The key regulatory gene YALI0D00451 was overexpressed, and a 5-aminolevulinic acid synthase gene was introduced to construct a 5-aminolevulinic acid synthesis pathway. This resulted in an engineered *Yarrowia lipolytica* strain with high methanol utilization, enabling the synthesis of the high-value-added chemical 5-aminolevulinic acid using methanol as the primary carbon source. This provides important strain resources and technical support for green biomanufacturing based on one-carbon resources.

The application provides a composite antibacterial biological preparation for preventing and treating tobacco brown spot, and a preparation method and application thereof, and belongs to the technical field of tobacco disease prevention and treatment.The composite antibacterial biological preparation comprises the following components in parts by weight: Chinese herbal medicine fermentation liquor 50-110 parts, active ingredient 8-25 parts, and auxiliary agent 1.5-3.8 parts; the active ingredient is a mixture of 5-amino levulinic acid hydrochloride and sinomenine and limonene at a mass ratio of 5-10:0.01-0.03:0.15-0.78.The application realizes effective prevention and treatment of tobacco brown spot through synergistic effect of multiple components, and the components are natural, easy to degrade, strong in rainwater erosion resistance, free of pesticide residues and environmentally friendly.

The application discloses a kind of compositions containing 5-amino levulinic acid and application thereof.The composition includes 5-amino levulinic acid and tetrahydropyrimidine.It is proved by test that in fruit swelling period and color changing period, the above-mentioned composition is sprayed on leaf surface, through the synergistic mode of "supply enhancement-transportation strengthening-synthesis stabilization", jointly promote fruit sugar accumulation and fruit peel coloring.Upgrade fruit quality.The above-mentioned composition can be applied to grape, apple, cherry, strawberry, pear, peach, apricot, tomato and other fruit quality improvement.It is proved by test that compared with single component, the composition of the application is obviously more excellent in the improvement effect on color and flavor of grape fruit, and has more excellent improvement effect on leaf agronomic index, single fruit weight, single cluster weight and nutrient content.

The present application provides a kind of fermentation method for improving the content of vitamin B12, by controlling total sugar content to be 1.0-7.0g / 100ml in the fermentation process, under the condition that 5-amino levulinic acid is added in the fermentation process of producing vitamin B12 in the fermentation medium by using Pseudomonas denitrificans, the metabolic pathway of vitamin B12 product synthesis is greatly strengthened.The fermentation method of the present application shortens the fermentation period and effectively improves the fermentation titer in the fermentation process of producing vitamin B12, and has great industrial application prospect.