34 results about "Amino-Levulinic Acid" patented technology

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

The objective is to provide 5-aminolevulinic acid or its salts and a method for producing the same, which have fewer impurities and residual solvents and suppressed discoloration compared to conventional methods. The objective is to provide 5-aminolevulinic acid or its salts, wherein, based on peak area obtained by HPLC analysis, the ratio of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0007 or less, and the sum of the ratios of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0016 or less.

This invention belongs to the field of agricultural herbicides and provides a photosynthetic inhibitor herbicidal composition comprising 5-aminolevulinic acid, malondialdehyde, and a herbicidal active ingredient. The herbicidal active ingredient is selected from one or a combination of several herbicides selected from acetolactate synthase inhibitors, photosynthetic system II inhibitors, or protoporphyrinogen oxidase (PPO) inhibitors. This invention utilizes the synergistic effect of 5-aminolevulinic acid, malondialdehyde, and a specific type of herbicidal active ingredient to effectively enhance the efficacy of the herbicidal active ingredient, enabling rapid and thorough weed control.

The object of the invention is to provide a pharmaceutical composition for enhancing the antitumor effect by an immune checkpoint inhibitor. Provided is a pharmaceutical composition for enhancing the antitumor effect by an immune checkpoint inhibitor comprising 5-aminolevulinic acids (ALAs) as the active ingredient.

The present application relates to the field of metabolic engineering and microbial fermentation, and in particular to a recombinant shewanella producing 5-amino levulinic acid and its application. The present application discloses a new 5-amino levulinic acid producing strain, which is obtained by modularly modifying shewanella by synergistically expressing 5-amino levulinic acid production module genes (hemA, hemL and gltX) derived from shewanella, antioxidant module genes (katE, sodB) derived from escherichia coli, and introducing inhibition modules (the inhibition genes are hemB2 and sucA), so that the shewanella can efficiently produce 5-amino levulinic acid, and the yield of 5-amino levulinic acid can be increased by 134.0 times compared with the original strain.

The application discloses a 5-aminoacetylpropionic acid-producing genetically recombined bacillus velezensis and application thereof, relates to the technical field of biotechnology and microbial metabolic engineering. The bacillus velezensis is obtained by integrating a hemA gene of Bradyrhizobium japonicum into the genome of bacillus velezensis R9, and the bacillus velezensis is obtained. The bacillus velezensis R9 has been preserved in the China Center for Type Culture Collection on July 5, 2023, and the preservation number is CCTCC NO: M 20231199. The strain can ferment to produce 17 mg / L of 5-aminoacetylpropionic acid in a 5-aminoacetylpropionic acid detection culture medium, and the concentration is more than 3 times higher than that of the bacillus velezensis R9, so the strain is an ideal engineering strain for producing 5-aminoacetylpropionic acid.

The present invention provides a composition comprising 5-aminolevulinic acid or a salt or ester thereof, comprising: (a) an active ingredient selected from 5-aminolevulinic acid or a salt or ester thereof; and (b) an aqueous carrier comprising an analgesic. The 5-aminolevulinic acid or the salt or ester thereof can be used for photodynamic therapy analgesia for more than 20 minutes, so that the clinical treatment cycle can be covered, the pain feeling after photodynamic therapy can be obviously relieved, the photodynamic therapy receiving time of a patient is prolonged, and the medication compliance of the patient is improved. Moreover, the composition provided by the invention can also improve the condition of tissue edema after photodynamic therapy, improve the skin smoothness after photodynamic therapy and shorten the pigmentation time, and meanwhile, compared with a marketed preparation, the composition has a better effect in the aspect of photodynamic therapy of inflammatory diseases (such as psoriasis or contact dermatitis) or acne and the like, and has a good application prospect. The acceptability of the patient to the photodynamic therapy is further improved; and the application scene of the photodynamic therapy is widened.

The invention provides a biological stimulant for regulating and controlling micro-ecological activity of acid soil rhizosphere as well as a preparation method and application of the biological stimulant, and particularly provides the biological stimulant which comprises high fructose corn syrup, tea polyphenol and 5-aminolevulinic acid. The biostimulant further comprises an amino acid and an oligosaccharide. The biological stimulant provided by the invention can improve the pH of rhizosphere soil, reduce exchangeable aluminum ions, improve soil respiration and mycorrhizal spore density, and realize yield increase and efficiency increase.

This application relates to the field of aquatic product logistics technology, and more particularly to an additive for improving the survival rate of yellow catfish during long-distance transportation and its preparation method. An additive for improving the survival rate of yellow catfish during long-distance transportation comprises the following raw materials: 0.2-0.5 parts of microbial preparation, 2-3 parts of glucose, 0.5-1 parts of compound vitamins, 3-5 parts of pH adjuster, 0.2-0.4 parts of 5-aminolevulinic acid, 0.8-1.2 parts of traditional Chinese medicine extract, and 91-95 parts of deionized water. The additive of this application, through the synergistic effect of microbial preparation, 5-aminolevulinic acid, and traditional Chinese medicine extract, improves hypoxia, improves water quality, enhances the immunity and stress resistance of yellow catfish, significantly increases the survival rate of yellow catfish during long-distance transportation, effectively solves the problem of low survival rate of yellow catfish during long-distance transportation, and can be widely used in the logistics and distribution of yellow catfish.

The invention relates to the field of functional feed, in particular to application of 5-aminolevulinic acid in preparation of products for preventing and treating ascites symptoms of broiler chickens, the products further contain bacillus coagulans BC001, and the preservation number of the bacillus coagulans BC001 is CGMCC No.26470. The invention further relates to application of the 5-aminolevulinic acid in preparation of the products for preventing and treating ascites symptoms of the broiler chickens. The addition amount of the 5-aminolevulinic acid or the pharmaceutically acceptable salt of the 5-aminolevulinic acid in the product is 5-1000g per ton of feed according to the addition amount of the 5-aminolevulinic acid in the feed; the product contains vitamin C and sodium selenite. It is found for the first time that 5-ALA has a remarkable effect on reducing the occurrence rate of broiler ascites syndrome, and the remarkable effect can be achieved by preferably adding 50 g of 5-ALA per ton of feed. 5-aminolevulinic acid, VC and sodium selenite synergistically act, so that the effect of preventing and treating the ascites syndrome of the broilers is better, and the morbidity and mortality of the ascites syndrome of the broilers can be remarkably reduced through synergism.

The present application relates to a kind of microdroplet photodynamic therapy compositions for treating skin and mucous membrane squamous cell carcinoma and its preparation method, belong to medical technical field.The microdroplet photodynamic therapy compositions for treating skin and mucous membrane squamous cell carcinoma, including photosensitizer precursor, anesthetic and solvent, the mass final concentration of the photosensitizer precursor is 15-25 %, the mass final concentration of the anesthetic is 4-6 %, and the photosensitizer precursor is hydrochloric acid aminolevulinic acid.The microdroplet photodynamic therapy compositions for treating skin and mucous membrane squamous cell carcinoma prepared by the present application adopts the mode of microdroplet injection, solves the three big problems of drug delivery, function preservation and pain control commonly faced in the treatment of different sensitive parts (face, hand, genital organ, tongue), enhances the practicability of the microdroplet photodynamic therapy compositions for treating skin and mucous membrane squamous cell carcinoma of the present application.

This invention provides an in-situ gel delivery carrier for 5-aminolevulinic acid and its preparation method, belonging to the field of biomedical technology. The in-situ gel delivery carrier comprises TAT ​​peptide and *Rhizopus oryzae* lipase; the mass-to-volume ratio of TAT peptide to *Rhizopus oryzae* lipase is 0.03-0.07 g: 0.005-0.015 g. This in-situ gel delivery carrier combines a dual mechanism of "physical thermosensitive phase transition" and "biological thermosensitive enzyme activity" to achieve long-term, targeted adhesion to lesion sites. Through the nonlinear synergistic effect of *Rhizopus oryzae* lipase and TAT peptide, it precisely degrades lipid barriers and efficiently delivers drugs, thereby significantly reducing the drug concentration while exponentially increasing the conversion efficiency of protoporphyrin IX (PpIX) in target cells. The preparation method is simple to operate.

The present application belongs to the technical field of synthetic biology and microbial metabolic engineering, and particularly relates to a high-yield 5-amino levulinic acid (ALA) strain, a screening method and application thereof. The present application discloses that 5-amino levulinic acid (ALA) causes higher levels of oxidative stress in a dose-dependent manner, and the oxidative stress caused by ALA reduces the intracellular cAMP level of Escherichia coli. Based on this, the present application constructs cAMP response promoters with different kinetic ranges, and establishes a high-throughput screening system of ALA high-yield strains on the basis. The present application uses the system to screen enzyme high-yield mutants in two ALA production pathways (C4 pathway and C5 pathway) through fluorescence-activated cell sorting (FACS), and successfully screens excellent mutant strains of ALA production pathway enzymes, and therefore has good practical application value.

The invention particularly relates to a composition and a method for long-distance transportation, storage and sowing of cyclobalanopsis glauca fruits. According to the specific technical scheme, the method for prolonging the preservation period of the cyclobalanopsis glauca fruits comprises the steps that the cyclobalanopsis glauca fruits are collected and put into an aqueous solution containing 5-aminolevulinic acid and abscisic acid, the fruits are taken out after being soaked, then the fruits are put into a sealed space, and 1-methylcyclopropene is introduced for fumigation. The invention provides a novel method for treating cyclobalanopsis glauca fruits, the method is low in cost and convenient to operate in the field, the period of validity of the treated fruits under the conventional logistics transportation condition can reach 20-25 days, and enough time is reserved for continuous collection and transportation.

This invention provides a 5-aminolevulinic acid producing strain, its construction method, and its application. Based on the existing strain JAL9, it involves knocking out… pflB、ldhA、poxB、adhE、ackA、dppA Genes, upregulated eamA、grxA、sodB The transcriptional level of the katE gene was downregulated. pfkB Gene transcription level, heterologous expression hemA ST Genes and xfp The gene, along with a high-copy plasmid PETC4, expresses genes from... Rhodobacter capsulatus of hemA RC Genes, ultimately through hemB Introducing an ASV degradation tag at the gene terminus enhances the degradation of HemB protein, effectively improving the synthesis efficiency of 5-aminolevulinic acid. This method has good industrial application value when applied to the fermentation production of 5-aminolevulinic acid.

The utility model discloses an instrument for accurately detecting the content of 5-aminolevulinic acid, which relates to the technical field of chemical detection and comprises a pretreatment tank, a treatment cavity is formed in the pretreatment tank, a rotating rod is arranged in the treatment cavity, a plurality of filter screen frames are uniformly and fixedly connected to the periphery of the rotating rod, and the filter screen frames are fixedly mounted at the bottom end of the rotating rod; fixing rods are fixedly connected to the two sides of the treatment cavity, and bristles are fixedly connected to the ends, close to each other, of the two fixing rods. According to the utility model, through the cooperation of the components, the outer wall of the filter screen frame is brushed by the bristles in the rotary filtering process of the filter screen frame, so that the bristles can remove impurities on the filter screen frame in time, the filter screen frame is prevented from being blocked by the impurities, the detection result deviation caused by impurity interference is avoided, and the detection accuracy is improved; due to the fact that the outer wall of the filter screen frame is cleaned in the rotating process of the filter screen frame, the automatic cleaning effect is achieved, manual intervention is reduced, and the labor intensity and the working risk of workers are reduced.

The invention discloses a synthetic method of 5-aminolevulinic acid and derivatives thereof, 5-aminolevulinic acid and derivatives thereof and application, and relates to the technical field of chemical synthesis, and the synthetic method comprises the following steps: mixing monoalkyl succinate, an inert solvent and an activating reagent, and carrying out acylation activating reaction to obtain a first intermediate; mixing the first intermediate, nitromethane, an alkaline agent, a molecular sieve and an organic solvent, and carrying out nitro condensation reaction to obtain a second intermediate; and mixing the second intermediate, a solvent and a catalyst, and carrying out catalytic hydrogenation reaction to obtain the 5-aminolevulinic acid and the derivative thereof. By adopting the steps, only three-step reaction is needed from the initial raw material to the target product, the reaction conditions are mild and efficient, the 5-aminolevulinic acid and the derivative thereof prepared by the process are white solids, the chemical purity of the 5-aminolevulinic acid and the derivative thereof can reach more than 96.0% and the yield can reach more than 70% through HPLC (High Performance Liquid Chromatography) analysis, and the requirements of industrial large-scale production are completely met.

The application provides a medical light guide gel with high light guide performance and a preparation method thereof. The medical light guide gel with high light guide performance comprises pure water, glycerol and carbomer, and further comprises aminolevulinic acid propyl ester hydrochloride. The aminolevulinic acid propyl ester hydrochloride is recrystallized, mixed with other components, stirred for 10-15 min, and then the product is prepared by standing. The technical scheme has the technical characteristics of high light penetration, and improves the treatment efficiency and the phototherapy effect.

This invention discloses an engineered *Yarrowia lipolytica* strain capable of methanol assimilation, its construction method, and its applications. By inserting AOX, CTA, DAK, and DAS genes into the genome of *Yarrowia lipolytica* strain BZ, a methanol assimilation pathway was constructed within the *Yarrowia lipolytica* metabolic network. The key regulatory gene YALI0D00451 was overexpressed, and a 5-aminolevulinic acid synthase gene was introduced to construct a 5-aminolevulinic acid synthesis pathway. This resulted in an engineered *Yarrowia lipolytica* strain with high methanol utilization, enabling the synthesis of the high-value-added chemical 5-aminolevulinic acid using methanol as the primary carbon source. This provides important strain resources and technical support for green biomanufacturing based on one-carbon resources.

The application provides a composite antibacterial biological preparation for preventing and treating tobacco brown spot, and a preparation method and application thereof, and belongs to the technical field of tobacco disease prevention and treatment.The composite antibacterial biological preparation comprises the following components in parts by weight: Chinese herbal medicine fermentation liquor 50-110 parts, active ingredient 8-25 parts, and auxiliary agent 1.5-3.8 parts; the active ingredient is a mixture of 5-amino levulinic acid hydrochloride and sinomenine and limonene at a mass ratio of 5-10:0.01-0.03:0.15-0.78.The application realizes effective prevention and treatment of tobacco brown spot through synergistic effect of multiple components, and the components are natural, easy to degrade, strong in rainwater erosion resistance, free of pesticide residues and environmentally friendly.

The invention relates to the technical field of fermentation preparation, in particular to a penaminolevulinic acid preparation device and a preparation method thereof.The penaminolevulinic acid preparation device comprises a fermentation vat, a clamping block is rotationally installed at the bottom of an inner cavity of the fermentation vat through a motor, a plurality of arc plates are arranged on the outer side of the clamping block, a rotating rod is detachably installed at the top of the clamping block, and a fermentation frame is arranged on the outer side of the bottom of the rotating rod; through the detachable connection design of the rotating rod and the clamping block, the whole device can be conveniently taken out of the fermentation barrel, thorough cleaning of the interior of the fermentation barrel is facilitated, the fermentation frame is used for fixing the filter cloth, solid-liquid separation of fermentation liquid is effectively achieved, and the stirring unit can conduct basic mixing and can also conduct stirring. A uniform, stable and efficient environment is created for microbial reaction, the arranged oxygen supply assembly works cooperatively with the adjusting assembly and the stirring unit, and oxygen is supplied synchronously in the stirring process, so that the gas-liquid contact area is remarkably increased, and the oxygen dissolution efficiency and the thallus utilization rate are improved.

An agent for ameliorating and / or preventing sickle cell disease, includes 5-aminolevulinic acid (ALA) or a derivative thereof or a salt thereof. The agent is useful for amelioration and / or prevention of sickle cell disease. The agent may include one or more kinds of metal-including compounds. The metal in the metal-including compound can be any of several different metals, including iron, magnesium, zinc, nickel, vanadium, copper, chromium, molybdenum, or cobalt.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a transcription factor MdWRKY71 or a MdWRKY71 gene in promoting anthocyanin accumulation in plants, wherein an amino acid sequence of the transcription factor MdWRKY71 is shown as SEQ ID NO:1, and a nucleotide sequence of the MdWRKY71 gene is shown as SEQ ID NO:2. The transcription factor MdWRKY71 is synthesized by the MdWRKY71 gene, and it is proved by transgenic and functional identification that the transcription factor MdWRKY71 synthesized by the MdWRKY71 gene as a new transcription factor for regulating anthocyanin accumulation in apples can positively regulate expression of key anthocyanin synthesis enzyme genes MdANS and MdDFR, and promote 5-amino levulinic acid (ALA)-induced anthocyanin accumulation in apples, and can play an important role in agricultural and forestry production and scientific research.

Provided are an aminolevulinic acid hydrochloride freeze-dried formulation and a preparation method thereof. With the character, moisture, related substances, and content of the aminolevulinic acid hydrochloride freeze-dried formulation as evaluation indicators, the processes such as temperature, rate, time, repeated freezing and thawing in the pre-freezing and sublimation process are explored. Meanwhile, the amplified freeze-drying process is optimized, and the character, appearance, and quality of the finished product after freeze drying all meet the requirements.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to low-pH-tolerant escherichia coli, a construction method of the low-pH-tolerant escherichia coli and application of the low-pH-tolerant escherichia coli in synthesis of 5-aminolevulinic acid. Specifically, escherichia coli MG1655 is taken as a starting strain, mutS genes are knocked out through a Red homologous recombination technology to improve the mutation rate, and an acid-resistant strain is obtained through adaptive laboratory evolution; meanwhile, an overexpression module plasmid containing hemA, coaA, ppc, eamA and grxA genes and an sRNA inhibition module plasmid containing targeted hemB, aceA and sucC genes are further constructed, and the double plasmids are transferred into the acid-resistant strain, so that the acid-resistant recombinant escherichia coli with excellent 5-aminolevulinic acid production performance is obtained. An efficient strain and a technical support are provided for industrial production of the 5-aminolevulinic acid, so that the 5-aminolevulinic acid strain has a good practical application value.

The application discloses a kind of compositions containing 5-amino levulinic acid and application thereof.The composition includes 5-amino levulinic acid and tetrahydropyrimidine.It is proved by test that in fruit swelling period and color changing period, the above-mentioned composition is sprayed on leaf surface, through the synergistic mode of "supply enhancement-transportation strengthening-synthesis stabilization", jointly promote fruit sugar accumulation and fruit peel coloring.Upgrade fruit quality.The above-mentioned composition can be applied to grape, apple, cherry, strawberry, pear, peach, apricot, tomato and other fruit quality improvement.It is proved by test that compared with single component, the composition of the application is obviously more excellent in the improvement effect on color and flavor of grape fruit, and has more excellent improvement effect on leaf agronomic index, single fruit weight, single cluster weight and nutrient content.

Disclosed is use of an MdPHY7 protein or an MdPHY7 gene related to apple anthocyanin biosynthesis in regulation of the apple anthocyanin biosynthesis, belonging to the technical field of plant genetic engineering. Exogenous 5-aminolevulinic acid (ALA) treatment can induce expression of the MdPHY7 gene, thereby promoting an increase of the apple anthocyanin biosynthesis, and then ultimately resulting in accumulation of apple anthocyanins to promote apple fruit coloring.

The invention discloses a drug loading system based on natural apoptotic bodies (AB) and application of the drug loading system in glioma photodynamic therapy. The drug loading system contains apoptotic bodies modified by targeted peptide MFGM, and the amino acid sequence of the targeted peptide MFGM is shown as SEQ ID NO. 2. Preferably, the drug loading system also contains 5-aminolevulinic acid (5-ALA). According to the invention, a drug with a relatively high concentration is directly wrapped in the MFGM modified AB, on one hand, ANG2 and RGD in the MFGM can enhance BBB penetration and tumor targeting, on the other hand, consumption of the drug and generation of by-products are also avoided, and on the other hand, the MFGM modified AB transfers the target drug into target cells through a membrane fusion effect, so that the limitation of transmembrane transport of some drugs is overcome, and the drug delivery efficiency is improved. And the concentration of the drug in target cells is enhanced. Meanwhile, for some drugs with relatively strong side effects, AB limits the drugs in vesicles for transportation, and has no toxicity to normal cells and low side effects.

The present application provides a kind of fermentation method for improving the content of vitamin B12, by controlling total sugar content to be 1.0-7.0g / 100ml in the fermentation process, under the condition that 5-amino levulinic acid is added in the fermentation process of producing vitamin B12 in the fermentation medium by using Pseudomonas denitrificans, the metabolic pathway of vitamin B12 product synthesis is greatly strengthened.The fermentation method of the present application shortens the fermentation period and effectively improves the fermentation titer in the fermentation process of producing vitamin B12, and has great industrial application prospect.

The purpose of the present invention is to provide 5-aminolevulinic acid or a salt thereof and a method for producing the same, the 5-aminolevulinic acid or salt thereof having suppressed discoloration and fewer impurities and residual solvents than in the prior art. Provided is 5-aminolevulinic acid or a salt thereof, in which the ratio of the content of individual impurities to the content of the 5-aminolevulinic acid or salt thereof is 0.0007 or less, and the total of the ratios of the contents of the individual impurities to the content of the 5-aminolevulinic acid or salt thereof is 0.0016 or less, based on the peak areas obtained by HPLC analysis.