207 results about "Amino-Levulinic Acid" patented technology

The invention relates to the technical field of synthetic biology and microbial fermentation, in particular to a 5-aminolevulinic acid synthetase mutant and application thereof. According to the invention, 5-aminolevulinic acid synthetase derived from rhodobacter capsulatus is expressed in recombinant Escherichia coli, point mutation is carried out on non-conservative amino acid of wild-type ALAS to obtain I86C mutants, the enzymatic activity of the 5-aminolevulinic acid synthetase is improved, and the ability of the 5-aminolevulinic acid synthetase in synthesis of 5-aminolevulinic acid is improved. The method can be used for solving the problems that the enzyme activity of the ALAS is low and the activity of the ALAS is easily inhibited by high-concentration substrate glycine and the like at present, and the yield of the 5-ALA is increased.

The invention discloses a transcription factor Cgl2988 related to production of 5-aminolevulinic acid based on network model prediction and application, the amino acid sequence of the transcription factor Cgl2988 is as shown in SEQ ID NO.1. The transcription factor Cgl2988 related to production of 5-ALA is simulated and predicted based on a metabolic network model system mcCGL, and experiments prove that the transcription factor Cgl2988 related to production of 5-ALA has the advantages that the transcription factor Cgl2988 related to production of 5-ALA can be used for preparing the transcription factor Cgl2988 related to production of 5-ALA; the expression strain Cgh3-low-cgl2988 of the transcription factor gene cgl2988 in the down-regulated corynebacterium glutamicum is subjected to shake flask fermentation, and the yield of 5-ALA is 5.52 + / -0.19 g / L and is increased by about 1.64 times compared with that of a control strain; the expression strain Cgh3-pX-cgl2988 of the transcription factor gene cgl2988 in the up-regulated corynebacterium glutamicum is subjected to shake flask fermentation, and the yield of the 5-ALA is 5.76 + / -0.11 g / L, which is about 2.84 times higher than that of a control strain.

The invention relates to the technical field of compound fertilizers, and discloses a boron-zinc-molybdenum synergistic compound fertilizer, which is prepared from the following raw materials in parts by weight: calcium nitrate, ammonium polyphosphate, potassium sulfate, magnesium sulfate, disodium octoborate tetrahydrate, sugar alcohol chelated zinc, sodium molybdate and a synergistic auxiliary agent, the feed additive is prepared from a feed additive, an activator, 5-alginic acid oligosaccharide, aminolevulinic acid, sugar alcohol chelated magnesium, an antagonist, a stress-resistant enhancer and a coating material. According to the boron-zinc-molybdenum synergistic interaction compound fertilizer and the preparation process thereof, the disodium octoborate tetrahydrate, the sugar alcohol chelated zinc and the sodium molybdate are compounded according to a specific proportion, antagonism is avoided through proportion limitation, the synergistic effect of the three elements is remarkably enhanced, and the problem that a single element is insufficient or excessive is solved; chelated elements such as sugar alcohol chelated zinc and sugar alcohol chelated magnesium are combined with an activating agent (humic acid chelated calcium and silica sol) to form a gel-state chelate, so that the trace elements can be prevented from being fixed by soil, and the transfer efficiency of the trace elements in crops is improved.

The invention discloses a special organic-inorganic compound fertilizer for improving the yield and quality of rice in a rice field, and belongs to the technical field of agricultural fertilizers. The compound fertilizer comprises a three-layer structure consisting of a porous core layer, a quick release coating layer and a biodegradable film, wherein the porous core layer is prepared from ammonium polyphosphate, potassium humate, nano charcoal modified chicken manure, mineral source potassium fulvate and sodium carboxymethyl cellulose-bentonite composite colloid; the quick-release coating layer is prepared from sulfur-resin double-layer coated urea, amino acid chelated zinc, alginic acid complex boron, silica sol, ammonium molybdate, 5-aminolevulinic acid and nano cerium oxide; the biodegradable film is prepared from a film forming solution and a bioactive microbial agent. Through the design of a three-layer structure and the combination of an organic-inorganic-microorganism synergistic technology, accurate matching of nutrient release and a rice fertilizer requirement rule is achieved, meanwhile, soil health and environmental protection are considered, and an innovative solution is provided for green high-yield rice planting.

The invention relates to the field of genetic engineering, in particular to a genetically engineered bacterium for efficiently synthesizing 5-aminolevulinic acid. According to the invention, a synthetic biological technology and a genetic engineering means are utilized, escherichia coli E.coli BL21 (DE3) is taken as an original strain, a C5 way of the escherichia coli E.coli BL21 (DE3) is modified, and degradation of glutamic acid is reduced by knocking out a gadA gene and replacing a promoter of an argE gene; the synthesis of the 5-aminolevulinic acid is increased by enhancing the expression of gdhA, hemAS and hemL genes, replacing promoters and increasing the carbon flux flowing to the 5-aminolevulinic acid; in addition, by knocking out acetic acid synthesis genes ackA, poxB and pta, the loss of carbon flux flowing to TCA circulation is reduced, so that the purpose of increasing the yield of 5-aminolevulinic acid is achieved.

A method of treating non-melanoma skin cancer in a patient in need thereof is provided. The method includes administering a hedgehog pathway inhibitor to the patient and applying to a region of skin on the patient, a topical composition comprising 5-aminolevulinic acid, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable excipient. The method further includes incubating the topical composition on the region of skin; and illuminating the region of skin with a light source at an irradiance density of 20 mW / cm2.

The utility model relates to the technical field of reaction kettles, and discloses a reaction kettle for biosynthesis of 5-aminolevulinic acid, which effectively solves the problem that bubbles floating to the liquid level are inconvenient to crush in the reaction process at present, and comprises a reaction kettle, a rotating shaft is rotatably mounted in the reaction kettle, and a plurality of stirring blades are arranged on the rotating shaft. The rotating shaft is rotationally connected with the middle of the top end of the reaction kettle, the top end of the rotating shaft is fixedly connected with an output shaft of the motor, the motor is fixedly installed at the top end of the reaction kettle, stirring blades are evenly installed at the bottom end of the outer side of the rotating shaft, and a bubble removing assembly is installed on the outer side of the rotating shaft and comprises a floating block arranged on the outer side of the rotating shaft in a sleeving mode. Under the action of buoyancy, the floating block always floats above the liquid level, bubbles of solutions with different liquid level heights can be conveniently removed, meanwhile, the floating block is in sliding connection with the guide strip through the guide groove, the floating block rotates along with the rotating shaft, pointed cones on the side rods make contact with the bubbles on the liquid level conveniently, and the bubble removing effect is improved.

The invention discloses a method for producing 5-aminolevulinic acid by inhibiting expression of a global transcriptional regulatory factor gene mcbR in corynebacterium glutamicum. The method comprises the following steps: (1) constructing a pCRISPRi-mid-McbR plasmid and a pCRISPRi-high-McbR plasmid; (2) respectively introducing the plasmids into corynebacterium glutamicum Cgh3 through electrotransfection, verifying by using a bacterial colony PCR (Polymerase Chain Reaction) mode to obtain an expression strain Cgh3-m-McbR or Cgh3-h-McbR for inhibiting a global transcriptional regulation factor gene mcbR in the corynebacterium glutamicum, and fermenting to obtain 5-aminolevulinic acid; experiments prove that compared with a control strain Cgh3-pXi, the strain for inhibiting the expression of the global transcriptional regulation factor gene mcbR in corynebacterium glutamicum has the advantage that the yield of 5-aminolevulinic acid is increased by 1.27 times or more after fermentation.

The invention belongs to the technical field of fertilizers, and particularly discloses coated 5-aminolevulinic acid as well as a preparation method and application thereof. The preparation method of the coated 5-aminolevulinic acid comprises the following steps: (1) melting: melting urea phosphate to obtain a coating agent; and (2) mixing: uniformly mixing the coating agent and 5-aminolevulinic acid powder in a high-tower granulator, carrying out spray granulation, and cooling and curing to obtain the coated 5-aminolevulinic acid. The preparation method disclosed by the invention is simple in process, and the prepared coated 5-aminolevulinic acid is good in stability and can promote the growth of chilies.

The invention provides an anti-aging composition and application thereof, the composition at least contains pyrroloquinoline quinone disodium salt, ergothioneine and 5-aminolevulinic acid or salt thereof in physiologically acceptable media, the composition contains PQQ, ergothioneine and 5-aminolevulinic acid (5-ALA) or salt thereof, the three components are compounded to achieve a synergistic effect, and the anti-aging effect is achieved. The dyeing intensity of beta-galactosidase is reduced, and the anti-aging effect is achieved.

The present invention belongs to the field of plant growth regulators, and specifically relates to a fruit coloring composition, a fruit coloring functional fertilizer and their applications. The fruit coloring composition comprises 1-aminocyclopropane carboxylic acid and a protective auxiliary agent, and the mass ratio of 1-aminocyclopropane carboxylic acid to the protective auxiliary agent is 1:0.1 to 200; the protective auxiliary agent is selected from 5-aminolevulinic acid, diethyl aminoethyl hexanoate or choline chloride. The present invention first discloses the use of protective auxiliary agents such as 5-aminolevulinic acid, which can effectively improve the adverse effects when ACC is applied alone, such as high dosage for coloring, easy to cause negative phenomena such as plant defoliation, fruit cracking, fruit drop and quality decline. When the effective application dosage is only 0.05 to 2.5 ppm, excellent coloring effect can be achieved. At the same time, the safety of exogenous application of ACC is effectively improved, and the planting effect of fruit and vegetable crops can be improved in aspects such as preventing fruit drop, increasing fruit sweetness, reducing phytotoxicity, and relieving the growth inhibition of stems, leaves and fruits.

The invention belongs to the technical field of plant regeneration through a tissue culture technology, and the classification number is A01H; the invention particularly relates to a radix fici simplicissimae seedling raising method and application thereof. The seedling raising method comprises the following steps: cleaning and disinfecting radix fici simplicissimae leaf tissues, and then airing for later use; the method comprises the following steps: soaking radix fici simplicissimae leaf tissues serving as explants in a germination-promoting induction culture medium at room temperature for 12-20 hours, wherein the germination-promoting induction culture medium is 5-aminolevulinic acid loaded modified nano-zeolite dispersion liquid; placing the soaked leaf tissues on a seedbed paved with nutrient soil according to the number of 1-2 leaf tissues in each hole for culture, and spraying water until the soil is moist at the same time; after leaf tissue culture is conducted for 10-20 days, a nutrient solution is sprayed every 3-5 days, spraying is conducted every time till soil is moist, and when seedlings are cultured to be 10-15 cm high, the seedlings are transplanted to a field. The prepared culture medium can improve the germination rate of the radix fici simplicissimae, promote the growth of seedlings and reduce the seedling culture cost.

The present invention relates to a 5-aminolevulinic acid derivative having improved storage stability and bioavailability, and a pharmaceutical composition comprising same, the present invention exhibiting pharmacokinetic characteristics in which 5-aminolevulinic acid is more effectively exposed to blood in the body, and alleviating side effects such as pain, erythema, swelling, edema, burning, itching, exfoliation, hyperpigmentation, and long-term irritation and hypersensitivity following treatment. In addition, the present invention relates to a 5-aminolevulinic acid derivative and a composition comprising same in which 5-aminolevulinic acid is highly effective while reducing the severity of side effects, and which can be used for photodynamic diagnosis and treatment.

The invention belongs to the technical field of biology, and particularly discloses application of several compositions based on methyl carboline acid in stress resistance and growth promotion of plants. The invention solves the problems of single function, poor effect, large dosage, high cost and the like of the plant regulator in the market, and the methyl carboline acid, paclobutrazol, alginate oligosaccharide and diethyl aminoethyl hexanoate are compounded for use under a specific concentration, so that the growth of crops can be obviously promoted, and the yield and quality of the crops can be improved. The methyl carboline acid and the gamma-aminobutyric acid are compounded for use under a specific concentration, so that the influence of saline-alkali stress on the growth of crops such as wheat and cucumber can be remarkably relieved; the methyl carboline acid and the 5-aminolevulinic acid are compounded for use under a specific concentration, so that the growth of crops such as tomatoes and peanuts at a low temperature can be promoted. The stress-resistant and growth-promoting composition provided by the invention can be used for remarkably improving the self resistance of plants under a proper concentration, effectively promoting the growth and development of the plants and improving the yield and quality of crops.

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

This invention discloses a lipid assembly loaded with 5-aminolevulinic acid and its preparation method. The lipid assembly is a hollow sphere with a core consisting of a hydrophilic phospholipid-chelating agent conjugate formed by chelation with a reactive phospholipid PEG reagent. The outer layer of the phospholipid-chelating agent conjugate is loaded with 5-aminolevulinic acid via hydrazone bonds, and then combined with a radionuclide as an internal light source excitation photosensitizer. The lipid assembly is loaded with 5-ALA via hydrazone bonds. Utilizing the acid-sensitive bond-breaking characteristics of hydrazone bonds and the prodrug properties of 5-ALA, combined with a radionuclide labeled on the surface of the lipid assembly as an internal light source excitation photosensitizer, this invention can overcome the limitations of external light penetration depth in traditional photodynamic therapy, achieving deep PDT treatment. Combined with chemotherapy drugs, it can minimize the toxic side effects on normal tissues during the combined use of radionuclides and photosensitizers in targeted therapy.

The objective is to provide 5-aminolevulinic acid or its salts and a method for producing the same, which have fewer impurities and residual solvents and suppressed discoloration compared to conventional methods. The objective is to provide 5-aminolevulinic acid or its salts, wherein, based on peak area obtained by HPLC analysis, the ratio of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0007 or less, and the sum of the ratios of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0016 or less.

The invention discloses a saccharomyces cerevisiae engineering bacterium for producing heme-binding protein as well as a construction method and application of the saccharomyces cerevisiae engineering bacterium. The construction method comprises the following steps: (1) by taking Saccharomyces cerevisiae CEN.PK2-1C as an original strain, optimizing a metabolic pathway, enhancing the supply of cytoplasm alpha-ketoglutaric acid (alpha-KG) and succinyl coenzyme A, and realizing the efficient accumulation of 5-aminolevulinic acid (ALA); (2) regulating and controlling the heme synthesis gene cluster in a time-sharing manner, and cooperatively expressing heme binding protein, so as to improve the assembly efficiency of heme (Heme); and (3) a secretion system is optimized, the secretion bottleneck of eukaryotic protein is broken through, and transmembrane transport and extracellular accumulation of the heme-binding protein are realized. Through the transformation of the combination strategy, an engineering strain for efficiently producing the heme-binding protein is obtained, and the yield of the bean hemoglobin in the saccharomyces cerevisiae is remarkably improved. Under the fermentation condition of a 5L fermentation tank, the secretory expression quantity of the bean hemoglobin reaches 286.6 mg / L.

The utility model relates to the technical field of reaction kettles, and discloses a special reaction kettle for synthesizing 5-aminolevulinic acid, which comprises a base, brackets are connected to the upper end of the base, a reaction kettle body is rotatably connected between the inner sides of the two brackets through a rotating shaft, a connecting shaft is rotatably connected to the lower part of the inner side of the bracket on one side, and the connecting shaft is rotatably connected to the lower part of the inner side of the bracket on the other side. According to the reaction kettle disclosed by the utility model, the connecting rod is driven to rotate by the motor, so that the shifting pin does circular motion, the swing arm swings back and forth around the connecting shaft along with the motion of the shifting pin in the lower movable groove, and then the swing arm drives the reaction kettle body to swing back and forth under the action of the upper movable groove and the protruding shaft, so that the reaction kettle body can swing back and forth. The reaction kettle body can swing back and forth while internal parts of the reaction kettle body drive materials to move, so that the motility of the materials is further improved, and the synthesis reaction efficiency is improved.

The invention relates to the technical field of chemical substance preparation, and particularly discloses preparation equipment and a preparation method of 5-aminolevulinate, the preparation equipment comprises a preparation table, and further comprises a feeding unit arranged on the preparation table; the screening unit is arranged below the feeding unit; through the arrangement of the feeding bin, crushing of raw materials can be achieved, meanwhile, the raw materials can be screened during crushing, secondary screening is conducted through the screening bin below after screening, meanwhile, through the arrangement of the first driving unit and the moving unit, the screening bin can be driven to move while feeding is conducted, and in the back-and-forth moving process, the screening efficiency is improved. The feeding bin and the screening bin are impacted by the moving strips to generate vibration, so that the screening efficiency of the feeding bin and the screening bin is improved, raw materials clamped on the screening holes and the screening plate can be vibrated off through impact, the raw materials can be cleaned without shutdown, the caked raw materials can be crushed in the impact process, and the screening efficiency is improved. And therefore, subsequent screening work is facilitated.

The invention provides a method for detecting chloroethane in hexyl aminolevulinate hydrochloride and application, the method comprises the following steps: preparing a reference solution and a test solution, the reference solution is a standard chloroethane solution, and the test solution is a hexyl aminolevulinate hydrochloride solution containing chloroethane; respectively injecting the reference solution and the test solution into a GC-MS (Gas Chromatography-Mass Spectrometer) by adopting a headspace sampling mode, and recording chromatograms; calculating the content of chloroethane in the test sample according to a peak area by an external standard method; the used gas chromatographic column is DB-FFAP, and the adopted gas chromatographic determination conditions are as follows: the flow of the chromatographic column is 1-10ml / min; the initial temperature of the column temperature is 25-50 DEG C, the initial temperature is maintained for 1-10 minutes, and the initial temperature is increased to 150-250 DEG C at the rate of 10-50 DEG C / min and maintained for 1-10 minutes; the temperature of a sample inlet is 150-30 DEG C; the sample size is 0.5 to 5 ml; the mass spectrum conditions are as follows: the operation time is 5-20 minutes; and the mass-to-charge ratio of chloroethane is 64 and 66. The method can be used for accurately and quickly detecting the content of chloroethane in hexyl aminolevulinate hydrochloride.

This invention belongs to the field of agricultural herbicides and provides a photosynthetic inhibitor herbicidal composition comprising 5-aminolevulinic acid, malondialdehyde, and a herbicidal active ingredient. The herbicidal active ingredient is selected from one or a combination of several herbicides selected from acetolactate synthase inhibitors, photosynthetic system II inhibitors, or protoporphyrinogen oxidase (PPO) inhibitors. This invention utilizes the synergistic effect of 5-aminolevulinic acid, malondialdehyde, and a specific type of herbicidal active ingredient to effectively enhance the efficacy of the herbicidal active ingredient, enabling rapid and thorough weed control.

The invention provides a high-yield strategy and application of a porphyrin compound. The strategy comprises: fermentative culturing a porphyrin compound producing strain to produce a porphyrin compound; wherein an additive selected from the group consisting of 5-aminolevulinic acid (5-ALA), glucose, glutamate, succinic acid, or a combination thereof, is added before the start of fermentation or during the fermentation. According to the technical scheme, the yield of the porphyrin compound can be remarkably increased, the cost is low, and operation is easy.

The invention discloses a method for electrically synthesizing 5-aminolevulinic acid by using pichia pastoris. According to the method, based on construction of pichia pastoris for reducing a glycine pathway and a 5-ALA synthesis pathway, a microbial electrochemical system is used for providing external electric energy to realize electric synthesis of 5-ALA. According to the method, pichia pastoris is selected as a research object, an efficient carbon sequestration glycine reduction pathway and a 5-ALA generation pathway are constructed in the earlier stage, the recombinant pichia pastoris is applied to a microbial electrochemical system, formic acid or CO2 is used as a carbon source, electric energy supplied by the microbial electrochemical system is used as an energy source, and the microbial electrochemical system is constructed. And the generation condition of the pichia pastoris 5-ALA under the electric energy condition is researched.

The invention relates to the technical field of plant cultivation and nutrition, and discloses a treatment method for enhancing coloring and sugar increasing of citrus fruits based on 5-aminolevulinic acid. And phosphite serving as a carbon immobilization catalyst and polyhydric alcohol serving as a product transfer accelerant are creatively compounded. The preparation method comprises the key step of accurately regulating and controlling the pH value of the system so as to ensure the stability and efficient absorption of the preparation. According to the application method, foliage spraying is carried out in specific time windows of the young fruit stage and the expansion stage of citrus, so that the effect of the preparation is matched with the key physiological rhythm of fruit development. Through the synergistic effect of the composition and the method, the photosynthetic whole chain of light harvesting-carbon sequestration-transportation is systematically optimized, the soluble solid content and the sucrose content of the citrus fruits can be remarkably increased, the color turning of the peel is effectively promoted, and the method is safe, stable and outstanding in effect.

The utility model relates to the technical field of content detection, and discloses an accurate detection device for the content of 5-aminolevulinic acid, which solves the problem that a 5-aminolevulinic acid sample and a reagent are not uniformly mixed at present, and comprises a base, a detection cylinder is arranged right above the base, a stirring mechanism is arranged between the base and the detection cylinder, and the stirring mechanism is connected with the detection cylinder. The stirring mechanism comprises an L-shaped frame fixed at the top of the base; according to the device, through the cooperation between the motor and the transmission shaft as well as between the conveyor belt and the stirring shaft, the stirring rod rotates to stir a solution in the detection cylinder, through the cooperation between the bevel gear I and the bevel gear II as well as the driven shaft, the disc rotates conveniently, and through the cooperation between the driving rod and the sleeve plate as well as the cooperation between the U-shaped round rod and the U-shaped plate, the detection efficiency is improved. The detection barrel can continuously move up and down conveniently, then a solution in the detection barrel can continuously move, and meanwhile, the position of a stirring rod in the detection barrel is changed, so that a 5-aminolevulinic acid sample and a reagent can be uniformly mixed conveniently.

The invention provides a separation microneedle of a loaded lipidosome nanoreactor as well as a preparation method and application of the separation microneedle, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: by taking phospholipid, cholesterol, tetramethoxysilane and 3-aminopropyltriethoxysilane as raw materials, entrapping cis-platinum, 5-aminolevulinic acid and catalase to prepare a liposome nano-reactor; polyvinylpyrrolidone, citric acid, sodium bicarbonate and regenerated silk cellulose are used as raw materials, a liposome nano reactor is dispersed in a microneedle body through a pouring method, and the microneedle capable of being rapidly separated is successfully prepared. Experimental research proves that the separation microneedle has good biocompatibility and mechanical properties, can effectively break through cuticle and realizes efficient transdermal delivery of a liposome nanoreactor. The liposome nanoreactor can be locally planted in deep tumor tissues, continuously supplies oxygen through enzymatic reaction, effectively improves the difficulty of insufficient oxygen supply in tumor photodynamic therapy, and has obvious advantages and wide prospects in the aspects of skin tumor photodynamic therapy application and the like.

The object of the invention is to provide a pharmaceutical composition for enhancing the antitumor effect by an immune checkpoint inhibitor. Provided is a pharmaceutical composition for enhancing the antitumor effect by an immune checkpoint inhibitor comprising 5-aminolevulinic acids (ALAs) as the active ingredient.

The present application relates to the field of metabolic engineering and microbial fermentation, and in particular to a recombinant shewanella producing 5-amino levulinic acid and its application. The present application discloses a new 5-amino levulinic acid producing strain, which is obtained by modularly modifying shewanella by synergistically expressing 5-amino levulinic acid production module genes (hemA, hemL and gltX) derived from shewanella, antioxidant module genes (katE, sodB) derived from escherichia coli, and introducing inhibition modules (the inhibition genes are hemB2 and sucA), so that the shewanella can efficiently produce 5-amino levulinic acid, and the yield of 5-amino levulinic acid can be increased by 134.0 times compared with the original strain.

The invention discloses saccharomyces cerevisiae for relieving heme feedback inhibition and strengthening heme supply and application, and belongs to the technical field of genetic engineering. The invention constructs a 5-aminolevulinic acid synthetase mutant capable of removing heme, and the yield of 5-aminolevulinic acid and the yield of heme of a strain H5 constructed by in-situ integration of the mutant in saccharomyces cerevisiae can be increased by 1.69 times and 2.99 times respectively compared with a control strain CEN.PK2-1C. According to the present invention, the HEM1I213K and the HEM13 are further integrated on the basis of the H5 strain in the multi-copy manner, such that the heme synthesis of the obtained heme-reinforced strain H25 is improved by 16.57 times compared with the CEN.PK2-1C, the yield of the synthesized rHb and the heme binding rate are improved by 22.9% and 39.2% compared with the CEN.PK2-1C added with 25 mg / L heme, and the heme supply-reinforced and high-activity human hemoglobin synthesis can be easily achieved.