74 results about "Protoporphyrinogen oxidase" patented technology

The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic and genetically engineered plant cells, plant tissue, seeds and plants which contain and express an herbicide resistant protoporphyrinogen oxidase such that they germinate from seed and grow in the presence of an amount of herbicide where the parent plant does not. Such plants are especially appropriate for use in agriculture or horticulture where herbicides are used to kill undesirable plants which might contaminate or compete with the transgenic plant of interest.

The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic plant cells, plant tissue, seeds and plants which contain and express an herbicide resistant protoporphyrinogen oxidase such that they germinate from seed and grow in the presence of an amount of herbicide where the parent plant does not. Such plants are especially appropriate for use in agriculture or horticulture where herbicides are used to kill undesirable plants which might contaminate or compete with the transgenic plant of interest.

A protoporphyrinogen oxidase tolerant to photobleaching herbicide and derivatives thereof, comprising a polypeptide having the amino acid sequence represented by SEQ ID No. 2 or mutated peptides derived therefrom by deletion, addition, substitution, etc. of one or more amino acids in the above amino acid sequence and having an activity substantially equivalent to that of the protoporphyrinogen oxidase. The acquisition of the novel protoporphyrinogen oxidase, which is highly tolerant to photobleaching herbicide and originates in plant, make it possible to construct plants highly tolerant to photobleaching herbicide via the expression of this enzyme in host plants.

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

A herbicidal composition which comprises a protoporphyrinogen oxidase inhibiting herbicidal active ingredient, a water-soluble herbicidal active ingredient, a water-soluble anionic surfactant, a polyoxyalkylenehydrocarbylamine, a water-immiscible organic solvent in which the protoporphyrinogen oxidase inhibiting herbicidal active ingredient cannot be substantially dissolved, a thickner and water, wherein the protoporphyrinogen oxidase inhibiting herbicidal active ingredient is suspended in the water dissolving the water-soluble herbicidal active ingredient as solid fine particles has excellent suspension stability.

The invention relates to the field of pesticides, particularly to a thiotriazinone isoxazoline compound, a preparation method and application thereof, a herbicide containing the thiotriazinone isoxazoline compound, and a protoporphyrinogen oxidase inhibitor, wherein the thiotriazinone-containing isoxazoline compound has a structure represented by a formula (I), and R<1> and R<2> are respectively and independently selected from H, -NH2, C1-C6 alkyl or C1-C6 halogenated alkyl and the like. The thiotriazinone isoxazoline compound provided by the invention can efficiently inhibit the activity of plant protoporphyrinogen oxidase, and has good herbicidal activity and crop safety.

The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

This invention relates generally to the detection of genetic differences among soybeans. More particularly, the invention relates to soybean quantitative trait loci (QTL) for tolerance to protoporphyrinogen oxidase inhibitors, to soybean plants possessing these QTLs, which map to a novel chromosomal region, and to genetic markers that are indicative of phenotypes associated with protoporphyrinogen oxidase inhibitor tolerance. Methods and compositions for use of these markers in genotyping of soybean and selection are also disclosed.

A method of controlling a weed, which exhibits high herbicidal activity, without causing significant phytotoxicity on switchgrass, miscanthus, giant reed, reed canarygrass, blue stem, erianthus, napier grass or spartina. A method is provided of controlling a weed at a place where switchgrass, miscanthus, giant reed, reed canarygrass, blue stem, erianthus, napier grass or spartina grows, including applying an effective amount of a protoporphyrinogen oxidase inhibiting compound as an active ingredient to a place where switchgrass, miscanthus, giant reed, reed canarygrass, blue stem, erianthus, napier grass or spartina grows or will grow; and the method of controlling a weed at a place where switchgrass, miscanthus, giant reed, reed canarygrass, blue stem, erianthus, napier grass or spartina grows or will grow, wherein the protoporphyrinogen oxidase inhibiting compound is one or more compounds selected from the group consisting of flumioxazin, sulfentrazone, saflufenacil, oxyfluorfen, fomesafen and a compound represented by the following formula (I):

The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

Herbicidal concentrate compositions containing a combination of herbicides are provided. In particular, the present invention relates to aqueous herbicidal concentrate compositions containing a particulate encapsulated acetanilide herbicide and a protoporphyrinogen oxidase inhibitor (PPO inhibitor).

A method for treating a microbial infection involves administering an effective amount of a compound of the formula:

wherein R₁ is H, alkyl, aryl, heteroaryl, R₂ is H, halogen, alkyl, aryl, heteroaryl, R₃ is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R₄ is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R₅ is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R₆ is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R₇ is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R₈ is —CR₃, O, S, wherein R₅ and R₆, R₇ and R₆, R₅ and R₄, R₄ and R₃ can cyclize forming a 3-10 member ring comprising C, O, S, and/or N optionally substituted with one or more R₃; and administering light therapy, such as a photodynamic therapy (PDT) light source.

The invention discloses a herbicide composition for preventing and eliminating garden cuscuta plants. The herbicide composition is prepared from a protoporphyrinogen oxidase herbicide and a systemic herbicide according to a mass ratio of 1:(1 to 30). The herbicide composition has excellent prevention and elimination effects on the garden cuscuta plants, and has the advantages of high efficiency, simplicity, convenience, safety on hosts, environment friendliness and low cost.

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

Protoporphyrinogen oxidase having an activity of imparting acifluorfen resistance and gene thereof are provided. Cyanobacterium protoporphyrinogen oxidase gene is identified by introducing a protoporphyrinogen oxidase gene of Arabidopsis into cyanobacterium, disrupting a cyanobacterium gene with a transposon, selecting a mutant strain in which protoporphyrinogen oxidase gene is disrupted, identifying the disrupted protoporphyrinogen oxidase gene, and isolating the disrupted protoporphyrinogen oxidase gene. This procedure is effective as a gene isolation technique when a protein derived from other organism species that is homologous to a known protein (e.g., protoporphyrinogen oxidase from cyanobacterium) can not be found in a gene database of the other species.

Protoporphyrinogen oxidase enzyme-inhibiting herbicides are useful in a method for controlling unwanted ground shoots of vines and other trunk vegetation. Of particular interest is the use of carfentrazone ethyl and certain metabolites thereof for control of unwanted ground shoots of vines and other trunk vegetation.

This invention relates generally to the detection of genetic differences among soybeans. More particularly, the invention relates to soybean quantitative trait loci (QTL) for tolerance to protoporphyrinogen oxidase inhibitors, to soybean plants possessing these QTLs, which map to a novel chromosomal region, and to genetic markers that are indicative of phenotypes associated with protoporphyrinogen oxidase inhibitor tolerance. Methods and compositions for use of these markers in genotyping of soybean and selection are also disclosed.

The present invention provides a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing a plant that comprises at least one nucleic acidcomprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention also provides plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.