408 results about "Transcription Factor Gene" patented technology

This invention generally relates to a method for developing, generating and selecting human embryonic stem (hES)-like pluripotent cells using transgenic expression of intronic microRNA-like RNA agents. More particularly, the present invention relates to a method and composition for generating a non-naturally occurring intron and its intronic components capable of being processed into mir-302-like RNA molecules in mammalian cells and thus inducing certain specific gene silencing effects on differentiation-related and fate-determinant genes of the cells, resulting in reprogramming the cells into a pluripotent embryonic stem (ES)-cell-like state. The ES-like cells so obtained are strongly express hES cell markers, such as Oct3/4, SSEA-3 and SSEA-4, and can be guided into various tissue cell types by treating certain hormones and/or growth factors under a feeder-free cell culture condition in vitro, which may be used for transplantation and gene therapies. Therefore, the present invention offers a simple, effective and safe gene manipulation approach for not only reprogramming somatic cells into ES-like pluripotent cells but also facilitating the maintenance of pluripotent and renewal properties of ES cells under a feeder-free cell culture condition, preventing the tedious retroviral insertion of four large transcription factor genes into one single cell as used in the previous iPS methods.

The present invention relates to an isolated transcription factor gene which is expressed in a maturing dicot seed and which encodes a transcription factor protein which targets a promoter of a gene encoding seed storage proteins. One group of such genes binds to 7S globulin (b-phaseolin) or lectin (PHA-L) promoters. Transcription factors identified as Pv Seed Factor-1 (PvSF1) and Vicilin-box Binding Protein-1 (VBP1) have been isolated. There is also disclosed a method for enhancing or reducing expression of seed storage protein, lectin or oil-protein genes in dicot seed crops comprising transforming a seed crop plant with the transcription factor gene of the invention.

The invention discloses a gene promoter of ginseng PgPDR3 responded by methyl jasmonate and an application thereof. The sequences of the gene promoter of the ginseng PgPDR3 are shown in SEQ ID NO.1, or are the DNA sequences of which the homology with SEQ ID NO.1 is over 99%. According to the invention, a Genomewalker technology is adopted to clone, later the sequences of the gene promoter of the PDR transport protein is obtained, and then the expression vector Pcambia1301-ProPDR3::GUS of the promoter is built; if the promoter in the transgenic tobacco drives the GUS to express, and can be controlled by ginsenoside, salicylic acid, gibberellins and abscisic acid, the promoter participates in the transport and accumulation of the ginsenoside, which provides a basis for cloning the gene of key control recording factor in the ginsenoside transport signal pathway and controlling the transport and accumulation of the terpenoid, such as ginsenoside; and fine plants containing rich ginsenoside can be obtained by using a method for controlling or improving the ginseng and other plants via the promoter or the derivative thereof.

The invention discloses application of panax japonicus transcription factor gene PjWRKY1, namely, application in improving the expression quantity of key enzyme genes in the biological synthesis of panax japonicus saponins, and increasing the content of saponins in panax japonicus callus. The nucleotide sequence of the PjWRKY1 gene is shown as SEQ ID NO: 1, and WRKY transcription factors are coded. Through the adoption of functional genomics and techniques related to metabolic engineering, a panax japonicus PjWRKY1 transcription factor is proved to have the effect of positively regulating the biological synthesis of panax japonicus saponins; when the panax japonicus PjWRKY1 transcription factor gene disclosed by the invention is established on a plant expression vector and transferred into the panax japonicus callus for over expression, the expression quantity of the key enzyme genes in the synthetic route of panax japonicus saponins is increased, and the yield of panax japonicus saponins is increased.

The invention particularly relates to a salt-tolerant and drought-resistant gene TaMYB33 of wheat and coding protein as well as application thereof, belonging to the technical field of biological gene engineering. The salt-tolerant and drought-resistant gene TaMYB33 of wheat is a nucleotide sequence SEQ ID No.1 in a sequence table. The invention has the beneficial effects that an MYB transcription factor gene TaMYB33 of wheat is obtained by cloning for the first time, and a mediation method of agrobacterium tumefaciens is used for transferring the gene into arabidopsis. The comparative analysis proves that compared with a wild arabidopsis plant, the salt tolerance and drought resistance of a transgenic arabidopsis plant containing the gene TaMYB33 provided by the invention are obviously improved.

The invention provides Gingko MADS-box transcription factor gene GbMADS9 for controlling blossoming of plants; the gene is acquired by subjecting cDNA as a template to PCR (polymerase chain reaction) amplification, wherein the cDNA is acquired by reverse transcription of total RNA of gingko female flowers. The gene has the advantages that the gene GbMADS9 is capable of controlling blossoming of plants and shortening juvenile phase of plants, and under induction of GA3, ABA, salt stress, drought stress and cold damage stress, the overexpressed gene GbMADS9 is capable of enhancing plant tolerance to osmotic stress; the gene is applicable to controlling the blossoming phase of plants.

The invention discloses a soybean holy bean 9# NAC transcription factor gene GmST1 and a plant expression vector of the gene GmST1 and further discloses application of the gene GmST1 in improving salt/drought resistance of arabidopsis thaliana and soybean plants. An experiment proves that compared with a non-transgenic plant, the salt/drought resistance of a transgenic plant is greatly improved. Therefore, the theory basis and the practice foundation are provided for improving the salt/drought resistance of plants through genetic engineering means. The soybean holy bean 9# NAC transcription factor gene GmST1 can be widely used for cultivating salt/drought resistant plant varieties.

The present invention relates to a nitrogen-regulated GATA transcription factor gene required for sugar sensing and the modulation of the expression of this gene to modulate a characteristic in a plant. The GATA transcription factor of the present invention is involved in regulating sugar sensing in plants and its expression is influenced by nitrogen status. Increased expression of this or substantially similar genes can produce plants with improved nitrogen utilization and increased yield.

The invention provides rice SBP-box transcription factor genes and application thereof. The rice SBP-box transcription factor genes comprise SEQ ID No.1 nucleotide sequences and SEQ ID No. 2 amino acid sequences, coding sequences with the transcription factors and carriers or hosts containing the coding sequences. The invention further relates to a method of improving salt resistance of plants and a method of choosing plants with high salt resistance. The invention provides a new method of improving and searching salt resistance of plants; and the new method has wide application prospects.

The invention relates to a grapevine powdery mildew resistance transcription factor gene VpRFP1 promoter sequence and application thereof. In the sequence, a full-length 1249bp promoter sequence of a Chinese wild vitis pseudoreticulata powdery mildew transcription factor gene VpRFP1 is cloned by adopting chromosomal walking in combination with a nested polymerase chain reaction (PCR) technology, wherein the sequence comprises 103 TATA-boxes and 27 CAAT-boxes, has 8 elements related to plant defense reaction, 14 photoreaction elements, 5 plant hormone response elements, and 14 other unknown elements. By adopting an agrobacterium tumefaciens-mediated instantaneously converted nicotiana benthamiana leaves analyzing method, the function of the Chinese wild vitis pseudoreticulata powdery mildew transcription factor gene VpRFP1 promoter proves that: the promoter has stronger promotion activity, can actively respond to pathogen invasion and disease resistant signal substances, and can improve the disease resistance and high temperature resistance of wheat, rice, potato, corn, soybean, rape and other plants through a transgenic method.

The invention discloses a gene sequence for synthesizing a relative transcription factor SmMYB by eggplant anthocyanidin, comprising a nucleotide sequence shown in 1st-1035th nucleotides in SEQ ID NO.1, wherein a polypeptide coded by the gene sequence comprises an amino acid sequence is shown in SEQ ID NO.2. The gene sequence in the invention is a key regulation and control gene for synthesizing eggplant anthocyanidin, the relative transcription factor SmMYB is synthesized by eggplant anthocyanidin. A downstream gene which is regulated and controlled by the gene sequence and is closely related to anthocyanidin synthesis, and other transcription factor genes manually acting with the gene sequence, are screened. The gene sequence in the invention is applied to obtain an eggplant kind with high anthocyanidin content and excellent resistance quality.

The invention belongs to the technical field of plant gene engineering, and relates to clone and application of a maize water-logging tolerance-related transcription factor gene zm-bRLZ. The nucleotide sequence of the gene is shown as SEQ ID No. 1, and the gene has a total length of 4,027bp and comprises 6 exons. The cDNA sequence of the gene is shown as SEQ ID No. 2, and 282 amino acids are encoded on the gene. In flooding stress, the expression of the gene in maize inbred line Hz32 seedling roots is up-regulated and the expression level of the gene in the Mo17 is kept unchanged. The in-vitro combination of a gene protein product and an antidiuretic hormone (ADH) promoter anaerobic response factor shows that the zm-bRLZ gene has a regulation and control effect on an anaerobic induced gene. A pair of cleaved amplified polymorphic sequence (CAPS) markers is developed by utilizing zm-bRLZ gene sequence difference of the maize water-logging tolerance inbred line Hz32 and high-sensitivity K12. The gene is positioned at a water-logging tolerance quantitative trait locus (QTL) peak part of 9.04bin by utilizing K12*Hz32 F2 group. A candidate gene correlation analysis proves that a zm-bRLZ gene promoter region and a plurality of single nucleotide polymorphism (SNP) sites at a 3'-untranslated region (3'-UTR) are all obviously associated with a plurality of water-logging tolerance indexes.

The invention provides a stress resistance ERF transcription factor gene derived from Brassica napus, a preparation method and usage thereof. The Brassica napus stress resistance ERF transcription factor gene is BnaERFB1-2-Hy15, the base sequence thereof is shown as SEQ ID No1. In the invention, the gene sequence of Brassica napus stress resistance ERF transcription factor is cloned from Brassica napus seedling by polymerase amplification technology, the obtained ERF transcription factor gene can be used in plant transformation to improve stress resistance of plants.

The invention discloses a method for inducing and culturing in vitro multi-potential stem cells, comprising the following steps: two transcription factors, i.e., Oct3/4 and Nanog, are transferred into audult cells, cultivated in cell induction culture solution under the condition of non-heterologous feeder cells, to induce and clone multi-potential stem cells; and the induced multi-potential stem cells are cloned in embryo stem cell culture solution, cultured and amplified under the condition of heterologous feeder cells, and the multipotentiality thereof is maintained. The multi-potential stem cells generated by the invention can avoid transplant rejection; the multi-potential stem cells induced by non-heterologous feeder cells has better clinical application value; the technology of adopting Oct3/4 and Nanog transcription factor genes or direct transfer of relevant protein is concise and has higher biological safety.

The present invention provides for the first time YY1, a transcription factor gene over-expressed and/or functionally overactive in human cancer. The present invention provides methods of diagnosing and providing a prognosis for cancer such as prostate cancer, as well as methods of drug discovery. YY1 is also a therapeutic target for treatment of cancer resistant to conventional and experimental cancer therapeutics. Inhibition of YY1 expression and/or activity sensitizes resistant tumor cells to cytotoxic treatments, including chemotherapy, radiation therapy, hormonal therapy, and immunotherapy.

The invention belongs to the field of technology related to plant molecular biology and transgenosis. Concretely, the invention relates to verification of high temperature resistance function of the Arabidopis thaliana heat shock transcription factor gene HSFA 2, and the application of the gene in high temperature resistance plant new species cultivation. According to the invention, a model plant Arabidopis thaliana is used as material, and a HSFA gene T-DNA is inserted into a homozygous mutant lines CSS 849143 for researching high temperature resistance function of the HSFA2 gene in plants. The experiment result displays that the HSFA2 gene knock-out mutant appears a sensitive phenotype with heat shock stress, and survival rate, conductivity and osmotic pressure and other physiological indexes are significantly or highly significantly different from those of the wild type; The invention instructs that the HSFA2 gene plays an important effect in the signal transduction pathway of plant response low nitrogen stress, and predicts that the excessive expression of the gene can improve the heat resistance capability of plant and has a larger economic value in the plant stress-resistance molecular breeding.

The invention discloses an NAC (N-acetyl-L-Cysteine) transcription factor gene GmST2 of soybean holy bean No.9 and a plant expression vector of the GmST2. The invention further discloses application of the gene GmST2 in improvement on the salt resistant/drought tolerance in arabidopsis thaliana and soybean plants. Provided by tests, the salt resistance/drought tolerance of a transgenic plant is greatly improved compared with that of a non transgenic plant and theoretical base and practice foundation for improving the salt resistance/drought tolerance of crops through genetic engineering means are provided. The NAC transcription factor gene GmST2 can be widely applied to cultivation of salt-resisting/drought tolerant plant varieties.

The present invention relates to the production of islet cells and insulin in a subject by providing for expression of an islet transcription factors in the pancreas of the subject, by for example, introduction of nucleic acid encoding the transcription factor neurogenin3 or a factor that induces neuorgenin3 expression. The present invention also relates to methods for using a islet transcription factor gene and the islet transcription factor polypeptide to alter cellular differentiation in culture or in vivo to produce new β-cells to treat patients with diabetes mellitus.

The invention discloses an ERF transcription factor related to the plant stress resistance and an encoding gene and application thereof. The invention firstly provides an ERF transcription factor gene LcERF056 separated from lotus japonicas, the amino acid sequence of the gene LcERF056 is shown as SEQ ID No.1, and the nucleotide sequence of the encoding transcription factor is shown as SEQ ID No.2. The invention further provides an ERF transcription factor structure domain which can be combined with a cis-acting element to start stress resistance response gene expression and is shown as SEQ ID No.3. Function analysis experiments prove that the resistance of a plant to high salt stress can be effectively enhanced or improved by overexpressing the LcERF056 gene in the plant, and therefore the protein encoded by the LcERF056 gene has the functions of the EFR transcription factor. The invention further provides application of the ERF transcription factor and the encoding gene on the aspects of enhancing the adversity stress resistance of the plant, breeding new transgenic plant varieties with the adversity stress resistance and the like.

The invention discloses a panax notoginseng transcription factor gene PnMYB1 and application thereof. The PnMYB1 gene has a base sequence shown as SEQ ID NO:1, and encodes MYB transcription factor. The invention adopts functional genomics and metabolic engineering related technologies to prove that panax notoginseng PnMYB1 transcription factor has the function of regulating the biosynthesis of saponins of panax notoginseng. The panax notoginseng PnMYB1 transcription factor gene is constructed on a plant expression vector and is transferred into panax notoginseng callus for overexpression, thus enhancing the expression level of key enzyme gene in the synthetic route of saponins of panax notoginseng and PnbHLH1 transcription factor in panax notoginseng, and increasing the content of total saponins of panax notoginseng and part of monomer saponin.

The invention belongs to the technical field of rice genetic engineering, and in particular relates to functions and application of a transcription factor gene OsMYB91 in an MYB family relevant to stress-tolerance. The gene has the nucleotide sequence length of 990 bp, and is induced by various stress conditions such as drought simulation, cold, salt, heat shock, and plant hormones including ABA, ACC, NAA, 6-BA, JA, SA and the like; under the natural growing condition, the plant height of an over-expression pant of the gene is obviously lowered than that of a wild plant and an inhibited plant; when a rooting culture medium containing mannitol and ABA is used for treating the seedlings of over-expression, RNAi and wild plants, the over-expression of the gene is discovered to be capable of increasing the resistance to mannitol, and meanwhile, the growth and the germination rate of the transgenic plant are both influenced by an exogenous ABA; measurement of physiological indexes relevant to stress-tolerance of different transgenic plants after the NaCl treatment shows that the gene has the function of reinforcing the resistance of the rice.