300 results about "Transcription Factor Gene" patented technology

The invention provides application of a transcription factor CsPIF7 gene in regulation and control of theanine synthesis and improvement of tea quality, and belongs to the technical field of agricultural molecular biology. The invention finds that high expression of CsPIF7 can activate expression of TS1 and GS1, so that more theanine can be accumulated in tea leaves; after the expression of CsPIF7 is reduced by a tea tree VIGS technology, the activation and expression of TS1 and GS1 can be reduced, so that the content of theanine in tea leaves is reduced. Furthermore, the tea tree transcription factor CsPIF7 is found to be capable of positively regulating and controlling expression of TS1 and GS1 by utilizing LUC verification, and can be used for regulating and controlling the content of theanine in tea trees and improving the quality of tea leaves.

The invention discloses a transcription factor Cgl2988 related to production of 5-aminolevulinic acid based on network model prediction and application, the amino acid sequence of the transcription factor Cgl2988 is as shown in SEQ ID NO.1. The transcription factor Cgl2988 related to production of 5-ALA is simulated and predicted based on a metabolic network model system mcCGL, and experiments prove that the transcription factor Cgl2988 related to production of 5-ALA has the advantages that the transcription factor Cgl2988 related to production of 5-ALA can be used for preparing the transcription factor Cgl2988 related to production of 5-ALA; the expression strain Cgh3-low-cgl2988 of the transcription factor gene cgl2988 in the down-regulated corynebacterium glutamicum is subjected to shake flask fermentation, and the yield of 5-ALA is 5.52 + / -0.19 g / L and is increased by about 1.64 times compared with that of a control strain; the expression strain Cgh3-pX-cgl2988 of the transcription factor gene cgl2988 in the up-regulated corynebacterium glutamicum is subjected to shake flask fermentation, and the yield of the 5-ALA is 5.76 + / -0.11 g / L, which is about 2.84 times higher than that of a control strain.

The invention discloses a heat shock transcription factor 49557 gene for increasing fucoxanthin content in phaeodactylum tricornutum and application thereof, and is characterized in that the nucleotide sequence of the 49557 gene is shown as SEQ ID NO: 3, the amino acid sequence of protein encoded by the 49557 gene is shown as SEQ ID NO: 4, the content of fucoxanthin synthesized by the 49557 gene is increased by knocking out the 49557 gene in the phaeodactylum tricornutum, and the content of fucoxanthin in the phaeodactylum tricornutum is increased. The method has the advantage that the fucoxanthin content in the phaeodactylum tricornutum can be remarkably increased.

The invention provides bacillus amyloliquefaciens as well as a construction method and application thereof. Wherein the bacillus amyloliquefaciens is the bacillus amyloliquefaciens of which the stress response transcription factor glcR gene is knocked out, and the bacillus amyloliquefaciens is a bacillus amyloliquefaciens of which the stress response transcription factor glcR gene is knocked out; the nucleotide sequence of the glcR gene is as shown in SEQ ID NO: 1. By knocking out a stress response transcription factor glcR gene in bacillus amyloliquefaciens, the level of glutaminase expression by using the bacillus amyloliquefaciens can be enhanced, the enzyme activity of the glutaminase obtained by expression is relatively high, and the activity of the enzyme can be further improved by using the modified strain; powerful technical support is provided for commercialized production of glutaminase, and great economic benefits and application prospects are achieved.

The invention discloses a polygonatum cyrtonema transcription factor gene PcWRKY1 as well as an encoding protein and application thereof. According to the application, a WRKY transcription factor PcWRKY1 which is obviously inhibited by high-temperature stress is screened, the transcription level of the PcWRKY1 transcription factor gene responding to the high-temperature stress is further determined, a tissue expression mode and subcellular sites of the PcWRKY1 transcription factor gene are analyzed, and finally, the regulation effect of the PcWRKY1 transcription factor gene on the high-temperature tolerance of the polygonatum cyrtonema protoplast is determined. The PcWRKY1 transcription factor gene is located in a cell nucleus, codes a protein containing 519 amino acids and is expressed in roots, stems, leaves, tubers and seeds of polygonatum cyrtonema, the expression quantity in the seeds is the highest, and the PcWRKY1 transcription factor gene can inhibit the increase of MDA content and promote the improvement of CAT enzyme activity under high temperature stress. Further, the tolerance of the polygonatum cyrtonema protoplast under high-temperature stress is improved.

The invention belongs to the field of plant genetic engineering, and discloses application of a PtNF-YB9 gene of trifoliate orange in drought-resistant genetic improvement of plants. The PtNF-YB9 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YB9 gene is as shown in SEQ ID NO. 1. The gene or the homologous gene thereof is knocked out or inhibited in a plant, the drought resistance of the plant is remarkably reduced, and the drought resistance of the plant is remarkably enhanced by overexpressing the gene or the homologous gene thereof. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an elongation factor subunit OsRAV9 gene for regulating and controlling resistance of rice to rice blast and application of the elongation factor subunit OsRAV9 gene. The elongation factor subunit OsRAV9 gene induced to express by magnaporthe oryzae is screened, and the nucleotide sequence of the elongation factor subunit OsRAV9 gene is as shown in SEQ ID NO: 1; the sequence of the protein coded by the gene is as shown in SEQ ID NO: 2. By means of agrobacterium tumefaciens-mediated transformation, an OsRAV9 over-expression transgenic strain and a CRISPR / Cas9 mutant strain are obtained. The rice blast inoculation identification on the transgenic material finds that the resistance of the OsRAV9 over-expression strain to the rice blast is enhanced, and the mutant strain is more susceptible to the rice blast bacteria, which indicates that the OsRAV9 is a positive regulation factor of the rice to the rice blast resistance. The OsRAV9 gene plays a positive regulation role in the rice blast resistance, and the resistance of rice to the rice blast can be remarkably improved by over-expression of the gene.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a wheat saline-alkaline tolerant ERF transcription factor gene TaERF109 and application thereof. The invention discloses the wheat saline-alkali stress response gene TaERF109 obtained by cloning by utilizing the existing plant gene engineering technology for the first time, the gene is overexpressed in wheat through an agrobacterium tumefaciens mediated method, and comparative analysis proves that the saline-alkali tolerance of a transgenic plant is obviously improved, and the transgenic plant has a good application prospect. And interference on expression of the TaERF109 can reduce the resistance of the wheat to salt and alkali stress. Therefore, the invention fully proves that the wheat saline-alkaline tolerant ERF transcription factor gene plays an important role in plant saline-alkaline tolerant regulation and control, and shows that the gene can be widely applied to cultivation and improvement of plant saline-alkaline tolerant varieties, so that the wheat saline-alkaline tolerant ERF transcription factor gene has good practical application value.

The invention discloses a panax notoginseng MYB transcription factor gene PnMYB8, the nucleotide sequence of the gene PnMYB8 is as shown in SEQ ID NO: 1, an MYB transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnMYB8 gene has the capability of improving fungal infection resistance of plants, the PnMYB8 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the panax notoginseng MYB transcription factor gene PnMYB8 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Cylindrocarpon destructor), the fusarium latericum (Fusarium latericum), the fusarium equiseti (Fusarium equiseti) and the phoma herbarum (Phoma herbarum) is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Fusarium latericum) is obviously enhanced.

The invention is suitable for the technical field of gene engineering, and provides a soybean MYB transcription factor gene and application thereof in increasing the content of isoflavone. The soybean MYB transcription factor gene is GmMYB3a, and the nucleotide sequence of the soybean MYB transcription factor gene is as shown in SEQ ID NO. 1. According to the invention, a pTF101-GmMYB3a overexpression vector and an RNAi-GmMYB3a interference vector are successfully constructed, and the constructed vectors are transferred into a receptor soybean variety Williams 82. Experimental results show that the soybean strain with overexpression of the GmMYB3a gene shows higher isoflavone content, while the soybean strain with interference of the GmMYB3a gene shows lower isoflavone content, and the positive regulation effect of the GmMYB3a in the isoflavone biosynthesis process is proved. The invention not only provides an innovative molecular breeding strategy for cultivating soybean strains with high isoflavone content, but also has remarkable application potential and market prospect.

The invention discloses a heat shock transcription factor bd1650 gene for increasing the content of fucoxanthin in phaeodactylum tricornutum and application thereof, and is characterized in that the nucleotide sequence of the bd1650 gene is as shown in SEQ ID NO: 1, the amino acid sequence of protein encoded by the bd1650 gene is as shown in SEQ ID NO: 2, the content of the fucoxanthin synthesized by the bd1650 gene is increased by knocking out the bd1650 gene in the phaeodactylum tricornutum, and the content of the fucoxanthin in the phaeodactylum tricornutum is increased. The method has the advantage that the fucoxanthin content in the phaeodactylum tricornutum can be remarkably increased.

The invention belongs to the field of plant genetic engineering, and particularly relates to a rice nitrogen response transcription factor OsNRTF2 gene, a protein coded by the rice nitrogen response transcription factor OsNRTF2 gene and application. The invention provides a rice nitrogen response transcription factor gene OsNRTF2, and the nucleotide sequence of the OsNRTF2 gene is shown as Seq ID No.1. The invention also provides a preparation method of the rice nitrogen response transcription factor gene OsNRTF2. The invention also provides an application of the gene OsNRTF2 at the same time, wherein the application is at least any one of the following applications: regulation and control of rice yield and nitrogen utilization efficiency. The invention provides important guidance for cultivating a new variety of nitrogen-efficient rice, and has important significance for guaranteeing grain safety.

The invention discloses application of a pear transcription factor PbrWHY2 gene in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrWHY2 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrWHY2 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. The PbrWHY2 can promote the growth of a pear pollen tube, the change of the expression of the PbrWHY2 is related to the change of ROS level and cellulose content at the top end of the pollen tube, and meanwhile, the PbrWHY2 also participates in a regulation mechanism of a self-incompatible reaction to regulate a downstream gene PbrAGP16 / PbrRALF6 / PbrGIS1 in a targeted manner. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention relates to a transcription factor gene specific fragment for delaying softening of postharvest apple fruits and a regulation and control method, and belongs to the technical field of plant genetic engineering. The MdWRKY33 transcription factor gene in an apple plant with poor storability is knocked out or silenced by using a transcription factor gene specific fragment as shown in SEQ ID NO.1 in a nucleotide sequence table, so as to delay the softening of postharvest apple fruits. The specific operation comprises the following steps: cloning a transcription factor gene specific fragment into a virus silencing vector pTRV2 to obtain a recombinant plasmid; respectively transferring the auxiliary vector pTRV1 and the recombinant plasmid into agrobacterium tumefaciens GV3101 strain cells by using a freeze thawing method to respectively obtain strain cells A and strain cells B; respectively carrying out culture medium culture and infection solution suspension treatment, and then preparing a mixed infection buffer solution; and immersing the apple fruits in the mixed infection buffer solution, and carrying out vacuum treatment to obtain the instantly silent apple fruits. Ethylene biosynthesis and cell wall degradation in the apple fruits are inhibited, and softening of the picked apple fruits is delayed.

The invention discloses a pseudo-ginseng bHLH transcription factor gene PnbHLH2, the nucleotide sequence of the pseudo-ginseng bHLH transcription factor gene PnbHLH2 is as shown in SEQ ID NO: 1, a bHLH transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnbHLH2 gene has the capability of improving fungal infection resistance of plants, the PnbHLH2 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the pseudo-ginseng bHLH transcription factor gene PnbHLH2 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to fusarium latericum, fusarium moniliforme, humicola fucoatra and cylindrocarpon destructor is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to the fusarium latericum, the fusarium moniliforme, the humicola fucoatra and the cylindrocarpon destructor is obviously enhanced. The transgenic tobacco overexpressed with the PnbHLH2 has the advantages that the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to the fusarium latericum, the fusarium moniliforme, the fusarium moniliforme and the cylindrocarpon destructor is obviously improved;

Disclosed are a Solanum tuberosum drought tolerance-related transcription factor gene StPIF4 and the use thereof. A CDS sequence of StPIF4 has a length of 1554 bp, has a nucleotide sequence shown as SEQ ID No. 1, and encodes 517 amino acids, the amino acid sequence being shown as SEQ ID No. 2. The gene is located in a cell nucleus. The gene reduces the water loss of Solanum tuberosum seedlings under the drought stress by adjusting the opening degree of stomas, thus enhancing the drought tolerance of Solanum tuberosum. The present invention provides a new genetic material and a theoretical basis for analyzing the drought-tolerant molecular mechanism and drought-tolerant breeding improvement of Solanum tuberosum.

The invention discloses a potato transcription factor gene bHLH47 and application thereof, and discloses that the potato bHLH47 gene participates in regulating and controlling the drought stress response of potatoes for the first time, and the drought resistance of the potatoes can be improved by reducing the expression quantity of the potato bHLH47 gene; the gene can be used for cultivating new transgenic drought-resistant varieties of potatoes, and has potential application value.

The invention is applicable to the technical fields of molecular biology and biology, and provides application of a vaccinium myrtillus MYB transcription factor VcMYB4a gene in improvement of vaccinium myrtillus salt tolerance. The nucleotide sequence of the VcMYB4a gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoded protein of the VcMYB4a gene is as shown in SEQ ID NO: 2. The recombinant RNAi plant expression vector contains a specific RNAi sequence aiming at the C2 negative regulation region of the VcMYB4a gene. The constructed recombinant RNAi plant expression vector is transferred into the bilberry callus, the expression of the C2 negative regulation region of the VcMYB4a gene is inhibited, the phenotype of the transgenic callus is observed after salt stress treatment, and the result shows that the inhibition of the expression of the C2 negative regulation region of the VcMYB4a gene improves the salt tolerance of the bilberry callus.

The invention discloses application of a scutellaria baicalensis SbMYB8 gene in promotion of anthocyanin synthesis, the application comprises the steps that the SbMYB8 gene is constructed to a plant expression vector and introduced into plant cells to promote synthesis of plant anthocyanin, and the nucleotide sequence of the SbMYB8 gene is shown as SEQ ID NO: 1, or the amino acid sequence coded by the SbMYB8 gene is shown as SEQ ID NO: 2. The MYB transcription factor SbMYB8 gene with the function of promoting anthocyanin synthesis is cloned and identified from the scutellaria baicalensis for the first time, and the blank of a transcription regulation mechanism for regulating anthocyanin metabolism in the scutellaria baicalensis is filled; the SbMYB8 gene can significantly promote accumulation of anthocyanin in various plants, shows good heterologous expression stability and adaptability, and has wide cross-species application potential.

The invention belongs to the technical field of biosynthesis of plant secondary metabolites, and particularly relates to a key gene for biosynthesis of large-fruit hawthorn flavonoid compounds and a screening method and application of the key gene. Through combined analysis of metabolome and transcriptome, a key gene combination containing seven structural genes and three transcription factor genes is screened out; the expression of the genes is remarkably positively correlated with the accumulation of a target flavone metabolite [6]-gingerol, and the genes are core factors for regulating and controlling the synthesis of the flavonoid compounds of the big-fruit hawthorns through experimental verification. According to the invention, the key gene for regulating and controlling the synthesis of flavone substances such as [6]-gingerol in the big hawthorn fruit is systematically identified for the first time, and a target spot is provided for analyzing a quality formation mechanism from a molecular level; the gene can be used for molecular marker-assisted breeding so as to cultivate a new variety of large-fruit hawthorn with high flavone content, and also can provide gene resources and technical support for the development of functional food and health care products.

The invention discloses application of a rice low-potassium response transcription factor KTR2. The sequence of the coding gene KTR2 of the low-potassium response transcription factor is shown as SEQ ID NO. 1. The gene can be applied to the aspects of controlling drought stress tolerance of rice and the like. The biological function of the low-potassium response transcription factor KTR2 in the rice is found through a large number of experiments, and after the gene is over-expressed, the tolerance of the rice to drought stress can be enhanced; after the gene is knocked out, the rice is sensitive to drought stress.

The invention provides a transcription factor knockout sinorhizobium meliloti strain as well as a construction method and application thereof. According to the application disclosed by the invention, transcription factor genes orf149, orf993, orf1177, orf1867, orf2327, orf2340, orf3280, orf3296, orf4169 and orf4207 are successfully knocked out in a genome of the sinorhizobium meliloti through a genetic engineering method, and a new strategy is provided for improving the yield of vitamin B12. Compared with wild-type sinorhizobium meliloti, the yield of vitamin B12 of the sinorhizobium meliloti recombinant strain constructed by the invention is increased by 50% or above at most.

The invention discloses application of a pear transcription factor PbrMADS1 to regulation and control of synthesis of stone cells and lignin as a component of the stone cells. A transcription factor PbrMADS1 gene separated from Dangshan pears belongs to SEP subfamily members, the nucleotide sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.1, and the coded amino acid sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.2 in a sequence table. The lignin content and the lignin synthetic gene expression quantity of the pear pulp callus with the PbrMADS1 being knocked out are remarkably reduced. Therefore, it is proved that the PbrMADS1 gene regulates formation of the pear fruit stone cells and the lignin component of the pear fruit stone cells. And the PbrMADS1 gene has the advantage of being capable of regulating and controlling a plurality of genes at the same time, so that a more efficient way is provided for molecular breeding. The discovery of the gene supplements and perfects a pear stone cell character transcriptional regulation mechanism, and provides a theoretical basis and gene resources for improving the pear fruit stone cell content.

The invention discloses a transcription factor ZmbHLH180 gene as well as an encoded protein and application thereof, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the corn ZmbHLH180 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2. The invention provides a transcription factor ZmbHLH180 gene, the CDS full length of the gene is 864bp, 287 amino acids are encoded, the gene is mainly positioned in a cell nucleus, and the expression of a chlorophyll degradation gene NYC1 and the senescence of leaves can be obviously promoted by over-expressing the ZmbHLH180 gene in arabidopsis thaliana; the trans-ZmbHLH180 gene overexpressed corn can remarkably promote leaf greening under drought stress, a certain technical foundation is laid for regulation and control of the plant growth cycle, a choice is provided for genetic improvement of new plant varieties, and the application value is important.

The invention discloses a silence system of a potato transcription factor StLRP1 gene and application of the silence system, and aims to regulate formation and development of potato tubers by silence of the StLRP1 gene so as to improve the yield and quality of the potato tubers. By silencing the StLRP1 gene, the tuberization efficiency of potatoes is remarkably improved, the tuberization time is advanced, early maturing is promoted, and the planting period is shortened. Meanwhile, the growth period is shortened due to gene silencing, the planting efficiency is enhanced, and the potatoes are more suitable for short growth seasons. In addition, the tubers of the plants are changed from an ovulate shape to a long cylinder shape, and the weight of a single potato plant is reduced, so that the storage and transportation efficiency can be improved, the market preference can be met, more choices are provided for breeding, and the cultivation of potato varieties adapting to different conditions is facilitated. Generally, the present invention enhances the production and adaptability of potatoes and brings advantages to the entire production chain of potatoes by improving the form and weight of tubers.

The invention provides application of an MsMYBS3 gene or a biological material thereof in regulation and control of plant cold resistance, and belongs to the technical field of plant genetic engineering. The amino acid sequence coded by the MsMYBS3 gene disclosed by the invention is as shown in SEQ ID NO. 4. The invention provides an application of a medicago sativa MYB transcription factor gene MsMYBS3 in the aspect of regulating and controlling the cold resistance of medicago sativa. A gene editing technology is adopted to directionally change the development and the cold resistance of the medicago sativa. The cold resistance of an MsMYBS3 gene editing strain constructed by the invention is reduced, and the MsMYBS3 gene has the effect of regulating and controlling the cold resistance of alfalfa, so that a theoretical basis and a candidate gene are provided for cultivating new varieties of high-resistance leguminous forage, and the application of alfalfa in production is promoted.

The invention discloses application of a potato transcription factor StBBM gene in potato tuberization breeding, and aims to regulate formation and development of potato tubers through silencing and overexpression of the StBBM gene so as to improve the yield and quality of the potato tubers. Through overexpression of the StBBM gene, the tuberization efficiency of the potatoes is remarkably improved, the tuberization time is advanced, early maturing is promoted, and the planting period is shortened. In addition, the tuber shape for silencing the gene is changed from an ovulate shape to a long cylinder shape, and the weight of a single potato plant is reduced, so that the storage and transportation efficiency can be improved, the market preference can be met, more choices are provided for breeding, and the cultivation of potato varieties adapting to different conditions is facilitated. Generally, the present invention enhances the production and adaptability of potatoes and brings advantages to the entire production chain of potatoes by improving the form and weight of tubers.

The invention discloses a wheat transcription factor gene TaWRKY65 and application of the wheat transcription factor gene TaWRKY65 in regulation and control of ethylene synthesis and drought resistance, and the nucleotide sequence of the wheat transcription factor gene TaWRKY65 is as shown in SEQ ID NO. 1. The wheat TaWRKY65 gene encodes a WRKY transcription factor, can be directly combined with a promoter of an ethylene synthesis gene TaACS, activates the expression of the wheat TaWRKY65 gene to promote the biosynthesis of ethylene, positively regulates the drought resistance of wheat, and has an important application value for drought-resistant breeding of wheat.

The invention discloses an application of a transcription factor gene MabHLH770 of Musa communis in improvement of drought tolerance of plants. The gene MabHLH770 is an alkaline helix-loop-helix (bHLH) transcription factor which is separated and cloned from bananas balsamifera, and the nucleotide sequence of the gene MabHLH770 is as shown in SEQ ID NO. 2. Research results show that after drought stress treatment, compared with a wild type, the silent strain is higher in relative conductivity, malondialdehyde content and hydrogen peroxide content, and lower in proline content and peroxidase activity. The transcription factor MabHLH770 can effectively improve the drought tolerance of the plant. The discovery of the gene provides a new gene resource for plant stress-resistant molecular breeding, provides reference for plant cultivation in arid regions, and is beneficial to improving the utilization rate of the arid regions.

The invention discloses a Pennisetum americanum transcription factor gene PgC3H11 for improving plant cold resistance and application thereof, and belongs to the technical field of gene engineering. The CDS sequence of the transcription factor gene PgC3H11 of the pennisetum americana is as shown in SEQ ID NO. 25. The zinc finger protein gene PgC3H11 is identified in pennisetum americana, the expression of the gene is induced by low-temperature stress, and the cold resistance of a transgenic plant can be enhanced by improving the expression level of the CBFs gene. The gene has important production value for improving the cold resistance of plants, creating excellent parent elements with high cold resistance and cultivating cold-resistant varieties.