84 results about "WRKY transcription factor" patented technology

The invention discloses application of panax japonicus transcription factor gene PjWRKY1, namely, application in improving the expression quantity of key enzyme genes in the biological synthesis of panax japonicus saponins, and increasing the content of saponins in panax japonicus callus. The nucleotide sequence of the PjWRKY1 gene is shown as SEQ ID NO: 1, and WRKY transcription factors are coded. Through the adoption of functional genomics and techniques related to metabolic engineering, a panax japonicus PjWRKY1 transcription factor is proved to have the effect of positively regulating the biological synthesis of panax japonicus saponins; when the panax japonicus PjWRKY1 transcription factor gene disclosed by the invention is established on a plant expression vector and transferred into the panax japonicus callus for over expression, the expression quantity of the key enzyme genes in the synthetic route of panax japonicus saponins is increased, and the yield of panax japonicus saponins is increased.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY4. The nucleotide sequence of the lilium regale WRKY transcription factor gene LrWRKY4 is described as in the SEQ ID NO:1,and the coded protein of the lilium regale WRKY transcription factor gene LrWRKY4 corresponds to the amino acid sequence described in the SEQ ID NO:2. According to the lilium regale WRKY transcriptionfactor gene LrWRKY4, it is proved that the LrWRKY4 gene has the function of improving the antifungal ability of plants through the technical research related to the functional genomics, the antifungal LrWRKY4 gene is constructed to a plant expression vector and transferred into tobacco for overexpression, the transgenic tobacco has very high antifungal activity, and the experimental result showsthat the tobacco with the LrWRKY4 gene overexpressed is highly resistant to infestation of nigrospora oryzae, fusarium graminearum, fusarium graminearum, botryosphaeria and fusarium solanum.

The invention relates to an induction-enhanced tissue specific promoter and application thereof, belonging to the technical field of plant genetic engineering. A promoter (BPHP, i.e. Bph14 promoter) for Oryza Sativa L. genes resistant to brown plant hoppers is separated from Oryza Sativa L. and is cloned. The promoter has a nucleotide sequence shown as SEQ ID No. 1, can control Oryza Sativa L. genes resistant to the brown plant hoppers to be specifically expressed in vascular bundle tissues and can be expressed in a way that the feeding induction of the brown plant hoppers is enhanced. The promoter contains BS1EGCCR cis-acting elements and can enable the genes to be specifically expressed in plant vascular bundle tissues. Moreover, the promoter has defense-related response cis-acting elements which can be combined with WRKY transcription factors, and the gene expression is increased when Oryza Sativa L. is fed by the brown plant hoppers or is infected by pathogenic bacteria. The promoter can be used as the promoter for the specific expression in the vascular bundle tissues and can be used as the brown plant hopper and pathogenic bacteria induction-enhanced promoter.

The invention discloses a Lilium regale Wilson WRKY transcription factor gene LrWRKY11. The Lilium regale Wilson WRKY transcription factor gene LrWRKY11 has a nucleotide sequence shown as SEQ ID NO: 1, and encodes a protein having an amino acid sequences shown as SEQ ID NO: 2. According to the invention, genomic studies have confirmed that the LrWRKY11 gene can improve anti-fungal functions of plants. Being constructed onto a plant expression vector and transferred into tobacco so as to be subjected to overexpression, the anti-fungal LrWRKY11 gene disclosed by the invention is capable of endowing the transgenic tobacco with very strong resistance to fungal pathogens. Experimental results have shown that transgenic tobacco with overexpression of the LrWRKY11 gene has very high level of resistance to infection of Nigrospora oryzae, Fusarium verticillioides, Botryosphaeria dothidea, Fusarium solani and Alternaria panax.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY3 and a preparation method thereof. A nucleotide sequence of the gene is shown as SEQ ID NO: 1, and a protein with an aminoacid sequence shown as shown in SEQ ID NO: 2; according to the invention, functional genomics related technical researches prove that the LrWRKY3 gene has the function of improving the antifungal performance of plants; the antifungal LrWRKY3 gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for overexpression; the transgenic tobacco plantshave very strong fungus infection resistance, and experimental results show that transgenic tobacco leaves with over-expression of LrWRKY3 have very strong resistance to infection of five pathogenicfungi such as alternaria oryzae, Botryosphaeria dothidea, fusarium graminearum, Fusarium rotunda and fusarium solani.

The invention discloses a myrothamnus flabellifolia gene MfWRKY17 and an application thereof. The nucleotide sequence of the gene is shown in SEQ ID NO. 1; The amino acid sequence of the protein encoded by the gene is shown in SEQ ID NO: 2. The invention improves the stress resistance performance of the plant and improves the stress resistance performance of the plant through the research on the drought resistance WRKY transcription factor of Milo.

The invention belongs to the technical field of plant gene engineering and specifically relates to a WRKY transcription factor for regulating and controlling aging of leaf blades and multiple stress tolerance and an application thereof. A plant WRKY6 protein provided by the invention is derived from arabidopsis, rape and other species, but is not limited to the species, and the plant WRKY6 protein comprises an analogous protein, wherein the homeology of the analogous protein with AtWRKY6 (the amino acid sequence is as shown in SEQ ID NO: 2) in the arabidopsis is higher than 30%. An encoding gene of the arabidopsis WRKY6 protein is one of the following nucleotide sequences: (1) SEQ ID NO: 1 in a sequence table; and (2) polynucleotide with the amino acid sequence as shown in the SEQ ID NO: 2 in a coded sequence table. The invention can provide a protein resource and a method in the aspects of deferring the aging of the leaf blades and improving the yield, quality and multiple stress tolerance of crops.

The invention belongs to the technical field of gene engineering, and discloses a camellia sinensis(L.)O.Kuntze) WRKY29 gene and an application thereof to improving cold resistance of plants. The nucleotide sequence of the camellia sinensis(L.)O.Kuntze) WRKY29 gene is shown as SEQ ID NO.1 in a sequence table. The amino acid sequence of the protein coded by the camellia sinensis(L.)O.Kuntze) WRKY29 gene is shown as SEQ ID NO.2 in the sequence table. The expression mode of the camellia sinensis(L.)O.Kuntze) WRKY29 is related to the response of camellia sinensis(L.)O.Kuntze) to low-temperature stress, the gene is over-expressed to transform plants, the cold resistance of the plants can be improved, the expression of CsWRKY29 is inhibited through an antisense oligonucleotide technology, and the damage of camellia sinensis(L.)O.Kuntze) leaves at low temperature is obviously aggravated. The cloning of the gene not only is beneficial to exploring the effect of a WRKY transcription factor in the cold resistance process of the camellia sinensis(L.)O.Kuntze), but also is beneficial to promoting the genetic improvement process aiming at enhancing the cold resistance of the camellia sinensis(L.)O.Kuntze) and promoting the sustainable development of the tea industry, so that the gene has a great application value.

The invention discloses a pseudo-ginseng WRKY transcription factor gene PnWRKY9 and the application thereof. The nucleotide sequence of the PnWRKY9 gene is as shown in SEQ ID NO: 1, and the PnWRKY9 gene encodes a WRKY transcription factor; according to the application, molecular biology and functional genomics related technical researches prove that the PnWRKY9 gene has the function of improving the fungal disease resistance of plants, the antifungal gene PnWRKY9 is constructed on a plant expression vector and transferred into tobacco for overexpression, and the transgenic tobacco plant has very strong in-vitro antifungal activity; according to the present invention, the PnWRKY9 overexpressed transgenic tobacco has the significant inhibition effect on the growth of Alternaria Compacta, Fusarium solani, and Nigrospora oryzae, and the PnWRKY9 overexpressed transgenic tobacco has the significant inhibition effect on the growth of the Alternaria Compacta, the Fusarium solani, and the Nigrospora oryzae, such that the PnWRKY9 overexpressed transgenic tobacco has the significant inhibition effect on the growth of the Nigrospora oryzae,.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to isolation and cloning, functional verification and application of an identified Gb-based WRKY1 factor in Sea Island cotton. The cDNA (Complementary Deoxyribonucleic Acid) sequence of an encoding gene of the Gb-based WRKY1 factor is as shown in SEQ ID NO: 1; and the amino acid sequence of the encoding gene is as shown in SEQ ID NO: 2. The invention also discloses a function of the gene (Gb WRKY1) for promoting the expression of the phosphate transporter; and the gene has the function of promoting the efficient utilization of arabidopsis thaliana phosphorus nutrition.

The invention relates to the technical fields of the molecular biology and the gene engineering, and concretely relates to a ZmWRKY58 gene obtained through the amplification in the total cDNA of a corn inbred line B73 by utilizing a PCR technology according to a transcription factor ZmWRKY58 gene sequence design primer. The gene codes a protein having one of amino acid residue sequences comprising SEQ ID No:1 in a sequence table, and an amino acid residue sequence obtained through substituting and/or deleting and/or adding 1-11 amino acid residues to the amino acid residue sequence represented by SEQ ID No:1 and having an interaction with a W box (TTGACC/T) cis-element to improve the stress resistances of plants. Results of onion cuticle subcell localization experiments show that the ZmWRKY58 protein has the nuclear localization characteristic of the transcription factor. The corn ZmWRKY58 gene is used for rice transformation, and obtained transgenic rice has an improved sensitivity to ABA, and has obviously-enhanced salt and drought tolerances. The corn ZmWRKY58 gene provides an important gene resource for the plant stress resistance gene engineering, and is of great significance to the improvement of the output of crops.

The invention relates to an atropa belladonna WRKY transcription factor gene and a recombinant plant expression vector and application thereof. The gene has a specific gene promoter activation role for synthesis of scopolamine, wherein the nucleotide sequence thereof is as shown in SEQ ID NO.3, and the coded amino acid sequence is as shown in SEQ ID NO.4. The AbWRKY1 gene is overexpressed in atropa belladonna, so that the content of scopolamine in atropa belladonna hairy root is increased, therefore, the gene can be applied to improvement of quality of the atropa belladonna, and can increase the content of scopolamine in the atropa belladonna.

The invention discloses a transcription factor relevant to blossoming of gossypium aridum, namely gossypium wild species gossypium aridum WRKY gene GarWRKY9, which is characterized in that the transcription factor has a sequence as SEQ ID NO. 2 (Sequence Identifier Number 2) shown in a sequence table. The protein GarWRKY9 relevant to the blossoming of gossypium aridum is separated by using electronic cloning and RT-PCR (Reverse Transcription-Polymerase Chain Reaction) technologies, functional verification is conducted by transgenic arabidopsis, and the transcription factor is proven to be able to advance the blossoming of a plant.

The invention discloses a tomato-resistant meloidogyne incognita related gene and application thereof. The invention provides a tomato-resistant meloidogyne incognita related gene of which the nucleotide sequence is as shown in SEQ ID NO.1, simultaneously provides protein encoded by the gene, and provides application thereof in enhancing the resistance of plants to meloidogyne incognita. A WRKY transcription factor encoding gene SlWRKY1 is separated from tomatoes, and the gene can be introduced into the tomatoes as a target gene to enhance the resistance of the tomatoes to meloidogyne incognita, thereby improving tomato varieties.

The invention discloses a pseudo-ginseng WRKY transcription factor gene PnWRKY15. The nucleotide sequence of which is as shown in SEQ ID NO: 1, the PnWRKY15 gene is proved to have a function of improving the antifungal function of plants through functional genomics related technical research, and the antifungal PnWRKY15 gene is constructed to a plant expression vector and transferred into tobacco for overexpression, transgenic tobacco plants have very strong fungal infection resistance, and experimental results show that the PnWRKY15 overexpression transgenic tobacco leaf total protein obviously inhibits mycelial growth of fusarium chlamydosporum and alternaria compacta.

The invention relates to the field of plant genetic engineering, provides a wheat WRKY transcription factor gene, and further relates to the application of the gene to transforming arabidopsis root development. According to the wheat WRKY transcription factor gene, the nucleotide sequence is embodied as SEQ ID NO:1. Transformation of arabidopsis roots of the gene is obviously changed, and the gene is involved in the plant development process.

The invention belongs to the technical field of genetic engineering, and particularly relates to the cloning of a Calycanthus praecox WRKY transcription factor gene CpWRKY71 and its promoter and an application thereof. The object of the present invention is to provide a new option for the study of adversity stress of Calycanthus praecox. The present invention provides the Calycanthus praecox WRKYtranscription factor gene CpWRKY71, and its encoded protein has an amino acid sequence shown as SEQ ID No.3. The Calycanthus praecox CpWRKY71 gene is cloned for the first time, and the expression characteristics of CpWRKY71 gene are detected by a real-time fluorescent quantitative PCR technology, and the function of the gene promoter is verified in Arabidopis thaliana by a transgenic technology.

The invention belongs to the technical field of genetic engineering, and relates to the cloning of a wheat salt-tolerant and drought-resistant WRKY transcription factor gene TaWRKY80 and application thereof. The invention discloses a wheat salt-tolerant WRKY transcription factor gene TaWRKY80 and application thereof in cultivating salt-tolerant plants. Experiments prove that: the salt tolerance of the transgenic plants is improved. The gene provided by the invention plays an important role in cultivating salt-tolerant plants (especially crops).

The invention relates to an application of a medicago sativa L. WRKY transcription factor in improving aluminum toxicity resistance and salt stress resistance of transgenic plants. 1, the WRKY transcription factor protein disclosed by the invention is a protein composed of an amino acid sequence shown in SEQ ID NO. 2; 2, the invention also provides a nucleotide sequence for encoding the protein, which is shown in SEQ ID NO.1; 3, the invention provides an application of the medicago sativa L. WRKY transcription factor protein and an encoding gene thereof in improving aluminum toxicity resistance and salt stress resistance of transgenic plants; 4, the invention provides a host cell containing the MsWRKY22 transcription factor gene and transformed by genetic engineering; and 5, the invention provides an application of an MsWRKY22 nucleotide sequence or an amino acid sequence or a cloning vector or an expression vector or a host cell in genetic engineering. The invention provides a theoretical basis for molecular breeding for improving aluminum toxicity resistance and salt stress resistance of medicago sativa L. by using a genetic engineering technology, and has very important application value.