Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Wheat WRKY transcription factor gene and application thereof to transforming arabidopsis root development

A transcription factor, Arabidopsis technology, applied to wheat WRKY transcription factor gene and its application in changing Arabidopsis root development

Inactive Publication Date: 2014-08-27
天津农兴源农业科技发展有限公司
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the involvement of wheat WRKY transcription factors in the regulation of plant root development

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Wheat WRKY transcription factor gene and application thereof to transforming arabidopsis root development
  • Wheat WRKY transcription factor gene and application thereof to transforming arabidopsis root development
  • Wheat WRKY transcription factor gene and application thereof to transforming arabidopsis root development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The present invention will be described in further detail below in conjunction with specific embodiments.

[0014] 1. Acquisition of wheat WRKY transcription factor gene TaWRKY72-b

[0015] 1. Extraction of total RNA: the leaves of wheat Yangmai 158 were used as the extraction material, and the TIANGEN kit was used, and the operation steps were as shown in the kit instructions. The obtained RNA solution was tested for RNA quality by agarose gel electrophoresis, and stored at -80°C.

[0016] 2. cDNA synthesis: cDNA was synthesized using Invitrogen's SuperScript TM ⅢFirst-Strand Synthesis System for RT-PCR, first take a 0.5ml sterilized microcentrifuge tube, add the following components respectively: 11μL of total RNA; 1μL of Oligo(dT); 1μL of 10mM dNTP, mix well, and shake the centrifuge tube gently to make the solution concentrate to the bottom , warm the mixture at 65°C for 5 minutes, then quickly place it on ice for 1 minute, then add the following reverse transcrip...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of plant genetic engineering, provides a wheat WRKY transcription factor gene, and further relates to the application of the gene to transforming arabidopsis root development. According to the wheat WRKY transcription factor gene, the nucleotide sequence is embodied as SEQ ID NO:1. Transformation of arabidopsis roots of the gene is obviously changed, and the gene is involved in the plant development process.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to a wheat WRKY transcription factor gene and its application in changing the root system development of Arabidopsis thaliana. Background technique [0002] Wheat is an important food crop in my country, and its stable and high yield is related to national food security, and the research on wheat is relatively comprehensive. The root system of wheat is firmly planted in the soil, which can provide the nutrients and water needed for growth, and is the basis for all biological activities such as wheat growth and development and response to adversity stress. However, compared with model crops, the current research on the mechanism of wheat root development is still very weak, and few regulatory genes have been identified. Therefore, it is difficult to adopt genetic manipulation methods to improve wheat root traits. Transcription factors control the expression of ge...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 谢晓东丁博李明王俊斌陈帅君白子彧
Owner 天津农兴源农业科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com