1276 results about "Triticeae" patented technology

A wheat variety designated 25R47, the plants and seeds of wheat variety 25R47, methods for producing a wheat plant produced by crossing the variety 25R47 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety 25R47 with another wheat line or plant, and the creation of variants by mutagenesis or transformation of variety 25R47. This invention also relates to methods for producing other wheat varieties or breeding lines derived from wheat variety 25R47 and to wheat varieties or breeding lines produced by those methods.

The invention discloses a method for mixed culture of crabs, shrimps and mandarin fishes. The method comprises the following steps: (1) water in a pool is 0.3-1.5m deep, the slope ratio of the pool is 1:3, high-protection facilities are built at the periphery of the pool, and an automatic aerator is arranged per 667m<2>; (2) the plant area of waterweeds in the pool accounts for one third of the area of the pool, and at the same time, snails are stocked; (3) at the end of March, 500-700 crabs per 667m<2> are stocked based on the standard of 80 crabs per kg, 5-7kg of post larvae of lobsters per 667m<2> are stocked in the first and middle third of April based on the standard, 7-10 mandarin fishes per 667m<2> (in 5 cm length) are put in the last third of May, and 6 kg of fingerling silver carps per 667m<2> are put in the first third of June based on the standard of 800-1000 fingerling silver carps per 1kg; (4) overhead price river crab feed cp.40% is used, supplementary with wheat and bean pulp, feed amount daily is controlled to 4-6% of the weight of shrimp and crab; and (5) a water quality regulator is used for one time per 15-30 days. On each day, shrimp and crab shell auxin and allicin, the amount of which account for 1-1.5% of the amount of feeds, are added.

The invention provides a wheat TaAGO4a gene CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats)/-CRISPR-associated protein 9) vector and application thereof and belongs to the field of crop molecular biology. The wheat TaAGO4a gene CRISPR/Cas9 vector and the application thereof have the advantages that gRNA of a third exon of specificity-targeted TaAGO4a is provided firstly, a DNA sequence of the gRNA is shown as SEQ ID NO.1, and the gRNA contains an enzyme cutting site XmnI; subsequently, the CRISPR/Cas9 vector containing the gRNA is provided, and through co-transformation of Cas9 and the specific gRNA into a wheat protoplast as well as enzyme cutting and sequencing technologies, the condition that the gRNA can guide the Cas9 to cut three copies positioned on a chromosome 3A, a chromosome 3B and a chromosome 3D of the TaAGO4a respectively can be detected successfully so as to cause frameshift mutation of the gene and result in afunction or excalation of the gene; the wheat TaAGO4a gene CRISPR/Cas9 vector can be used for preparing TaAGO4a gene-deleted transgenic wheat.

Three choline utilizing strains of microorganisms isolated from the anthers of wheat, Aureobasidium pullulans strain AS 55.2, Arthrobacter species strain OH 221.3, and Pseudomonas species strain AS 64.4, are superior antagonists of F. graminearum. These microorganisms are effective for suppression and control of FHB in cereals, particularly in wheat and barley.

A wheat variety designated 25R78, the plants and seeds of wheat variety 25R78, methods for producing a wheat plant produced by crossing the variety 25R78 with itself or with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety 25R78 with another wheat line or plant, and the creation of variants by mutagenesis or transformation of variety 25R78. This invention also relates to methods for producing other wheat varieties or breeding lines derived from wheat variety 25R78 and to wheat varieties or breeding lines produced by those methods.

The invention provides a Pseudomonas.chlororaphis subsp.Aurantiaca Pa40, preservation number of which is CGMCCNo.2764. The strain generates HCN, prolease and siderophore relative to antibacterium and has plate board inhibition effect on a majority of tested plant pathogenic fungi and a part of tested plant pathogenic bacteria. Control effect of the biocontrol strain Pa40 on rhizoctonia solani is detected by taking wheat as an indicator plant on the plate, in the greenhouses and in the fields; a result shows that the control effects of the Pa40 on the rhizoctonia solani on the plate bed, in the greenhouse and in the fields are respectively 53.97%, 68.10% and 72.46%; wherein the control effects on the rhizoctonia solani in the greenhouses and fields are higher than that of validamycin.

The invention relates to a high-yield cultivation technique for summer corns and particularly introduces measures for summer corn high-yield cultivation from the aspects of purple sweet potato and summer corn seed selection, seed sowing, fertilization, field management and the like. High-yield summer corn varieties are selected. After wheat is harvested, early sowing against time is conducted. During sowing, five kilograms of diammonium phosphate and one kilogram of zinc sulfate are applied per mu land. During fertilization, seeds and fertilizers are required to be separated strictly to avoid the situation of seedling wilting. Then, water is sprayed to keep a full stand of seedlings. The seedlings are required to be thinned, complemented and fixed in time when three visible leaves grow from the corn seedlings. According to the condition of soil fertility, three times of additional fertilization are conducted at the seedling stage, the heading stage and the flowering to maturity stage of corns to ensure corn production, the corns are irrigated in time and attention is required to be paid to water drainage at a later stage. Decis, phoxim and the like are sprayed to prevent corn smut diseases, head smut, armyworms, corn borers, red spiders and the like. After the corns are ripe, the corns are required to be harvested and air-dried in time.

The invention relates to application and preparation of bacillus amyloliquefaciens subsp. plantarum and a bacterial agent thereof. An XH-9 bacterial strain is identified as bacillus amyloliquefaciens subsp. plantarum according to mycelial morphology, colony characteristics, physiological and biochemical indexes and 16S rDNA sequence analysis and is preserved in the China General Microbiological Culture Collection Center, and a preservation number is CGMCC NO.13151. The strain is high in antagonistic effect on pathogenic fungi such as fusarium oxysporum, bipolaris sorokiniana, fusarium pseudograminearum, colletotrichum gloeosporioides, botryosphaeria dothidea and alternaria alternata and is capable of generating heteroauxin and 1-aminocyclopropanecarboxylic acid (ACC) deaminase to stimulate plant growth and capable of degrading celluloses and can be stably colonized at rhizospheres of crops such as wheat, corn, peppers and the like. The bacterial agent prepared from the bacterial strain can be applied to prevention and treatment of root rot diseases or other soil-borne fungal diseases of crops such as wheat, corn, peppers and the like and has dual functions of disease prevention and growth promotion. The bacterial agent is simple in preparation process, short in fermentation period, low in cost and beneficial to industrial production and transport.

The invention relates to a balanced fertilization method under the condition of wheat/corn one-year-two-harvest cultivation total straw returning. In the winter wheat season, corn ears are harvested when corn is ripe, straw and root remains are smashed, or the straw and the root remains are smashed while the corn ears are harvested by a machine, the corn straw and the root remains are smashed once again before soil preparation, base fertilizer is applied, soil is subjected to rotary tillage or is subjected to ploughing to bury the corn straw, then rotary tillage is conducted, wheat is sown, and topdressing is carried out in the jointing stage; in the summer corn season, wheat is harvested by the machine when being ripe, meanwhile, wheat straw is smashed, the wheat straw is made to cover the field, then corn is subjected to no-till machine seeding or manual dibble seeding, wheat stubble cleaning is carried out at the corn seedling stage, topdressing is performed at the belltop stage and the anthesis maturity period, or the corn is subjected to no-till machine seeding, meanwhile, seed fertilizer is applied, and topdressing is performed at the belltop stage and the anthesis maturity period. According to the balanced fertilization method, wheat and corn are used as one fertilization unit, the fertilizing amount and the fertilizing method are balanced, the full stand and healthy seedling and the high and stable yield of wheat and corn are guaranteed, soil organic matter is increased, the soil fertility is raised, sustainable highyield is achieved, and the fertilizer utilization efficiency is improved.

The invention relates to a method for enzymolyzing wheat bran to prepare asafetida oligosaccharide, wherein it uses wheat bran as raw material; uses enzymatical to remove amidon, remove protein to prepare wheat bran insoluble fiber; then uses bacillus subtilis xylanase to hydrolyze wheat bran insoluble fiber to prepare the asafetida oligosaccharide. And the density of asafetida oligosaccharide will reach 1.497mmol/L. the invention can effectively utilize wheat bran, while prepared asafetida oligosaccharide has significant biological activity, to accelerate the generation of bifidobacteria, and restrain the erythrocyte oxidisability damage indused by free group, with better social and economic benefits.

The present invention relates to an artificial planting technique of haloxylon ammodendron cistanche salsa. Said technique is formed from three portions of sowing of haloxylon ammodendron cistanche salsa host, reforestation of haloxylon ammodendron cistanche salsa host and inoculation of haloxylon ammodendron cistanche salsa. The cistanche salsa is parasitized in the root system of haloxylon ammodendron and grown, in the fourth and fifth month of second year said haloxylon ammodendron cistanche salsa can be dug up.

The invention discloses Siam bacillus and application thereof to prevention and control of fusarium graminearum. The collection number of the disclosed Siam bacillus is CGMCC No.9501. As a biological prevention and control material of wheat scab, the Siam bacillus has a very good application prospect on the aspect of development of novel bio-pesticides or biological prevention and control microbial agents.

The invention provides pseudomonas chlororaphis for preventing and treating the crop fusarium disease and applications thereof. The preservation number of the pseudomonas chlororaphis is CGMCC No. 7729. The Pcho01 bacterium strain of the pseudomonas chlororaphis has a high effective antagonistic action, is capable of being applied to the preventing and treating wheat scab caused by fusarium, and the preventing and treating efficiency in the field is maintained above 60%. The Pcho01 bacterium strain also has a very good antagonistic action on botrytis cinerea, rice sheath blight fungus, bipolaris maydis, pythium wltmum, rhizopus, and pseudomonas solanacearum, and can be used to prevent and treat the diseases caused by those bacteria mentioned above.

The invention relates to hawthorn dried beef, which consists of the following raw materials by weight part: 120-130 of beef, 150-160 of cola, 130-150 of hawthorn, 40-50 of smoked plum, 30-40 of sunflower seed kernel, 20-25 of walnut kernel, 10-15 of laver powder, 3-5 of Chinese wolfberry powder, 70-80 of flour, 4-6 of wheat germ, 4-6 of prunella vulgaris, 3-5 of safflower, 3-5 of cactus, 2-3 of Rabdosia serra, 3-5 of lettuce seeds, and 2-3 of rape flower, etc. The preparation process of the hawthorn dried beef provided by the invention is simple. Added with laver powder, walnut and other food materials, the hawthorn dried beef has rich nutrition and good taste. Also added with wheat germ, prunella vulgaris, safflower, cactus and other Chinese herb extracts, the hawthorn dried beef has certain health care efficacy, and is suitable for most people to eat.

The invention relates to the technical field of feeds and particularly relates to a lamb feed. The feed is prepared from the following raw materials in parts by weight: 75-80 parts of corns, 15-16 parts of sunflower seed meal, 10-12 parts of soya bean meal, 8-10 parts of broken rice, 15-18 parts of black wheat bran, 20-22 parts of wheat straws, 12-15 parts of dry straws, 5-8 parts of grass seed meal, 8-10 parts of mung bean leaves, 8-10 parts of peanut seedlings, 6-8 parts of sugar beet earthnuts, 4-5 parts of oenothera biennis seeds, 2-3 parts of Chinese chive seeds, 2-3 parts of fresh ginger powder, 8-10 parts of pea seedlings, 6-8 parts of crowndaisy chrysanthemum, 6-8 parts of bamboo shoots, a suitable amount of table salt and 0.2-0.5 part of a phagostimulant. The lamb feed provided by the invention is enriched with nutrient elements including amino acids, vitamins, microelements and the like needed by the rapid growth and development of lambs; after the lambs eat the feed, the growth and development of the lambs are rapid and the weight of the lambs is increased obviously.

The invention discloses a method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi to bring the reciprocal symbiosis relation between AM fungi and host plants under drought stress into play reasonably and efficiently. According to the method, the AM fungi having a close symbiotic relation with the wheat are selectively used, and the symbiotic relation between the AM fungi and the wheat is built in a condition control mode to promote the wheat growth and improve drought adaptability. The AM fungi comprise one of glomus monosporum and acaulospora laevis, the two kinds of AM fungi are respectively propagated in an expanding mode in an expanding propagation matrix by using red clovers, and then two AM fungi inoculants and the wheat are cultured together in a symbiotic culture medium. The effective path for profitable symbiosis of the AM fungi and the host plants in adversity conditions is provided, the infection and symbiosis relation with the host plants of the AM fungi is improved in a man-made mode, and the yield of dry crops is improved.

The invention provides a breeding method for the germ plasm of a new translocation line wheat-rye T2BL.1RS, which is characterized in that, distant hybridization is performed by taking common wheat variety Xiaoyan 6 as female parent and relative plant rye variety German White as male parent; seeds are acquired after immature embryo salvation culture, colchicine half root soaking method and chromosome doubling; backcross seeds are acquired after disease resistance identification, cytological identification and backcrossing utilizing the original parent Xiaoyan 6 as male parent; then after disease resistance identification and cytological identification, individual plants with excellent comprehensive characters are selected in direction for self crossing, and after continuous self cross selective breeding for six generations, the finally acquired individual plant is confirmed to be wheat-rye T2BL.1RS translocation line after identified by continuous total genome in situ hybridization and three-probe multicolor fluorescence in situ hybridization. The breeding method for the germ plasm of a new translocation line wheat-rye T2BL.1RS has the advantages that, the wheat-rye T2BL.1RS translocation line has high resistance to stripe rust, current prevalent fungus strain, powdery mildew and other important virus strains; the invention has excellent high yield character; the method lays an excellent germplasm base for the cultivation of breakthrough new wheat variety with disease resistance and high yield.

The invention relates to a method for cultivating winter wheat. The method comprises the following steps: soaking seeds into oxygenated micrometer/nanometer bubbly water of which the dissolved oxygen concentration is 40-60mg/L; intensively ploughing and preparing soil before sowing seeds; applying a base fertilizer, additionally fertilizing at the later stage of wheat jointing, spraying a leaf fertilizer at the booting stage of the wheat, spraying a biological fertilizer at the head sprouting stage of the wheat, and spraying a water-retaining fertilizer at the filling period of the wheat. The cultivating method is easy in operation, and is low in cost; the seed quality is enhanced, and the problems concerned with high-quality cultivation of wheat, resource utilization of agricultural waste and sustainable development of agriculture are solved.

The invention provides an application of application of plant alpha-diastase in pollen abortion, and belongs to the technical field of genetic engineering. Through introducing polynucleotide of the plant alpha-diastase, recombinant vector and recombinant vector are structured; a pollen specific promoter drives the plant alpha-diastase to degrade starch in advance during the pollen developmental phase; the pollen sprouting energy cannot be provided, and the pollen sprouting is contained, thus abortion is generated; the transgenetic seedling of transgene pollen abortion is obtained. The plant alpha-diastase can be used as the pollen abortion gene and can be originated from genes of rice endogenesis, millet, broomcorn, barley, wheat and other gramineous plants; the plant alpha-diastase is very good for transgenic plant of pollen abortion. The plant alpha-diastase gene has accurate expression level and can control the spreading of transgenosis; the plant alpha-diastase gene also can keep and breed a male sterility line while leave out the artificial castration step during the hybrid seed production process; the application prospect is wide.