Siam bacillus and application thereof to prevention and control of fusarium graminearum

A technology of bacillus and fusarium, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of increasing difficulty in prevention and control, and there is no ideal high-resistant wheat variety, and achieve the effect of good application prospects

Active Publication Date: 2015-02-11
北京中农探味科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For many years, the control of wheat head blight has mainly relied on chemical pesticides, but with the environmental problems caused by chemical pesticides, coupled with years of use of pesticides has led to the emergence of pathogenic bact

Method used

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  • Siam bacillus and application thereof to prevention and control of fusarium graminearum
  • Siam bacillus and application thereof to prevention and control of fusarium graminearum
  • Siam bacillus and application thereof to prevention and control of fusarium graminearum

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Experimental program
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Embodiment 1

[0029] The isolation and identification of embodiment 1 bacteria

[0030] 1. Isolation of bacteria

[0031] (1) In June 2012, 10 ears of diseased wheat ears collected in Jiangsu were placed in 100 ml sterile distilled water for 15 minutes to prepare a bacterial suspension in an ultra-clean workbench, and then treated in a water bath at 80°C for 10 minutes.

[0032] (2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water, spread it on the NA medium plate, and cultivate it at 30°C for 24 hours. The colonies cover the entire plate. Use an inoculation loop to pick out the shape, size, Strains with different colors and transparency were streaked and purified on the plate, and the purified strains were applied to the confrontation culture experiment of Fusarium graminearum (such as figure 1 shown). The obtained strain with strong antagonistic ability against Fusarium graminearum was named JS29I.

[0033] 2. Identification

[0034] (1) Accordi...

Embodiment 2B

[0044]Antagonism of Example 2 Bacillus siamensis JS29I to Fusarium graminearum

[0045] The antagonistic effect of Bacillus siamensisJS29I on Fusarium graminearum was detected by confrontation culture method.

[0046] 1. The cultivated pathogenic fungus Fusarium graminearum (Fusarium graminearum) FG was cultured with a hole puncher with a diameter of 5 mm. 1 Take the plate from the edge of the colony, and pick the plate into the center of the PDA plate with a sterilized toothpick.

[0047] 2. Inoculate the same volume and concentration of JS29I on a four-dot dash line at a distance of 25mm from the center of the PDA plate. Take the PDA plate only inoculated with the plate of Fusarium graminearum as the control group, culture at 28°C for 4-5 days, and observe whether there is antibacterial effect bands formed. Do three parallels for each group. The results are shown in Table 1. It shows that JS29I has a strong inhibitory effect on Fusarium graminearum.

[0048] The inhibit...

Embodiment 3J

[0051] The inhibitory effect of embodiment 3JS29I fermented liquid to Fusarium graminearum

[0052] The inhibitory effect of Bacillus siamensis JS29I on Fusarium graminearum was detected by confrontation culture method.

[0053] 1. The cultivated pathogenic fungus Fusarium graminearum (Fusarium graminearum) FG was cultured with a hole puncher with a diameter of 5 mm. 1 Take the plate from the edge of the colony, and pick the plate into the center of the PDA plate with a sterilized toothpick.

[0054] 2. Inoculate JS29I with the same volume and concentration at four points 25mm away from the center of the PDA plate. Take the PDA plate inoculated only with the plate of Fusarium graminearum as the control group, culture at 28°C for 4-5 days, and observe whether there is any antibacterial effect. bands formed. Do three parallels for each group. The results are shown in Table 1. It shows that JS29I has a strong inhibitory effect on Fusarium graminearum.

[0055] Table 2 The in...

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Abstract

The invention discloses Siam bacillus and application thereof to prevention and control of fusarium graminearum. The collection number of the disclosed Siam bacillus is CGMCC No.9501. As a biological prevention and control material of wheat scab, the Siam bacillus has a very good application prospect on the aspect of development of novel bio-pesticides or biological prevention and control microbial agents.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a siamese bacillus, and also relates to a siamese bacillus and its application in preventing and treating Fusarium graminearum. Background technique [0002] Fusarium graminearum is one of the important pathogens of gramineous crops, which can cause Fusarium Head Blight (FHB). Fusarium graminearum is a major factor limiting wheat yield. my country's climatic conditions are very favorable for the occurrence of wheat head blight, and the perennial disease in the middle and lower reaches of the Yangtze River caused a yield loss of 10%-15%, and a production reduction of nearly 50% in a pandemic year. Due to the impact of global warming and changes in farming systems such as straw returning to the field and no-tillage cultivation, the occurrence area of ​​wheat head blight in my country has rapidly expanded from the Yangtze River Basin to Northwest and North China, especially ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 赵月菊刘阳邢福国周露王龑
Owner 北京中农探味科技有限公司
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