Wheat-rye T2BL.1RS translocation line germplasm breeding method

Abstract

The invention provides a breeding method for the germ plasm of a new translocation line wheat-rye T2BL.1RS, which is characterized in that, distant hybridization is performed by taking common wheat variety Xiaoyan 6 as female parent and relative plant rye variety German White as male parent; seeds are acquired after immature embryo salvation culture, colchicine half root soaking method and chromosome doubling; backcross seeds are acquired after disease resistance identification, cytological identification and backcrossing utilizing the original parent Xiaoyan 6 as male parent; then after disease resistance identification and cytological identification, individual plants with excellent comprehensive characters are selected in direction for self crossing, and after continuous self cross selective breeding for six generations, the finally acquired individual plant is confirmed to be wheat-rye T2BL.1RS translocation line after identified by continuous total genome in situ hybridization and three-probe multicolor fluorescence in situ hybridization. The breeding method for the germ plasm of a new translocation line wheat-rye T2BL.1RS has the advantages that, the wheat-rye T2BL.1RS translocation line has high resistance to stripe rust, current prevalent fungus strain, powdery mildew and other important virus strains; the invention has excellent high yield character; the method lays an excellent germplasm base for the cultivation of breakthrough new wheat variety with disease resistance and high yield.

Description

technical field [0001] The invention relates to a germplasm breeding method of a wheat-rye T2BL.1RS new translocation line, which creates germplasm resources with excellent disease resistance and high-yield traits, and belongs to the field of agricultural biotechnology breeding research. Background technique [0002] The breakthrough of wheat breeding depends on the discovery and utilization of excellent germplasm gene resources. However, with the improvement of modern agricultural farming system, the genetic variation within cultivated wheat species has decreased sharply, and excellent germplasm resources are increasingly scarce. Numerous studies have shown that wheat relatives contain many rich and valuable genetic resources. Through distant hybridization, exogenous good genes are introduced into cultivated wheat species to broaden the genetic basis of wheat, which can not only create new species, but also breed new types with certain good characteristics. Therefore, the...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the main planted T1BL.1RS translocation varieties are gradually losing resistance due to the emergence of new pathogenic races (bacteria), i

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