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Wheat Gene taspl20-7d Molecular Marker and Its Application in Identification of Wheat Yield-related Traits

A technology for yield-related traits and molecular markers, which can be used in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., and can solve problems such as laborious and time-consuming breeding models

Active Publication Date: 2019-08-09
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional breeding mode through phenotypic screening is time-consuming and laborious, but the development of functional markers provides a more convenient and quick method for the selection of excellent lines in the process of wheat breeding

Method used

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  • Wheat Gene taspl20-7d Molecular Marker and Its Application in Identification of Wheat Yield-related Traits
  • Wheat Gene taspl20-7d Molecular Marker and Its Application in Identification of Wheat Yield-related Traits
  • Wheat Gene taspl20-7d Molecular Marker and Its Application in Identification of Wheat Yield-related Traits

Examples

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Effect test

Embodiment 1

[0109] Example 1. Wheat set of molecular markers is correlated with wheat yield-related traits

[0110] 1. Discovery of dCAPS-7D-SNP1 and CAPS-7D-SNP2

[0111] The inventor of the present invention has discovered two wheat molecules related to wheat thousand-grain weight and plant height according to 32 common hexaploid wheat varieties in Table 1 (from the National Germplasm Resource Bank, the public can obtain from the National Germplasm Resource Bank) Markers, these two wheat molecular markers were named dCAPS-7D-SNP1 and CAPS-7D-SNP2 respectively, dCAPS-7D-SNP1 and CAPS-7D-SNP2 were composed to obtain a set of wheat molecular markers, and dCAPS-7D-SNP1 was the wheat genome The DNA corresponds to the 646th nucleotide of sequence 1 in the sequence listing, dCAPS-7D-SNP1 is A or C, and CAPS-7D-SNP2 corresponds to the 765th nucleotide of sequence 1 in the sequence listing in wheat genomic DNA Nucleotide, CAPS-7D-SNP2 is C or T.

[0112] Table 1, 32 common hexaploid wheat vari...

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Abstract

The invention discloses a wheat gene TaSPL20-7D molecular marker and an application thereof in identifying wheat yield related characters. The wheat gene TaSPL20-7D molecular marker is a wheat molecular marker dCAPS-7D-SNP1 and a complete wheat molecular marker; the wheat gene TaSPL20-7D molecular marker is nucleotide at site 646, which corresponds to a sequence 1 in a sequence table, in a wheat genome DNA (Deoxyribose Nucleic Acid); the complete wheat molecular maker is composed of a wheat molecular maker dCAPS-7D-SNP1 and a wheat molecular maker CAPS-7D-SNP2; and CAPS-7D-SNP2 is nucleotide at site 765, which corresponds to the sequence 1 in the sequence table, in wheat genome DNA. According to the experiments, dCAPS-7D-SNP1 and the complete wheat molecular marker are associated with thousand seed weight and yield of wheat.

Description

technical field [0001] The invention relates to the field of biotechnology, the wheat gene TaSPL20-7D molecular marker and its application in identifying wheat yield-related traits. Background technique [0002] Wheat is a worldwide food crop, and using various approaches to increase wheat yield has become one of the main goals of many scientists, including breeders. The traditional breeding mode through phenotypic screening is time-consuming and laborious, but the development of functional markers provides a more convenient and quick method for the selection of excellent lines in the process of wheat breeding. With the development of molecular biotechnology, the functions of important genes have been continuously revealed, and the efficient use of these genes in crop genetic improvement has become an important goal of gene discovery. Molecular marker-assisted selection technology provides a new effective way to improve the selection efficiency of target traits and improve ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 景蕊莲徐伟娜张斌刘霞昌小平
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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