32 results about "Oryza rufipogon" patented technology

The embodiment of the invention provides a rice blast disease grade intelligent evaluation and disease resistance preliminary screening method, system and equipment, and the method comprises the steps: S1, collecting image data of wild rice leaves infected with rice blast, carrying out the instance segmentation of the image data through an improved YOLOv8-Seg segmentation model, obtaining a segmentation result, and carrying out the segmentation of the image data through an improved YOLOv8-Seg segmentation model; the improvement measures comprise a GhostHierachicalNet backbone network, a CAHSFPN neck network and an LSDES Head segmentation head, and the LSDES Head segmentation head is connected with the CAHSFPN neck network and the LSDES Head segmentation head; s2, according to the segmentation result, counting the number and proportion of pixels of diseased leaves and disease areas, and obtaining a disease grade evaluation result in combination with disease resistance identification technical regulations; and S3, screening wild rice materials with strong disease resistance based on the disease grade evaluation results which are repeated for multiple times. The method can effectively improve the accuracy and efficiency of crop disease evaluation, and provides support for large-scale screening of disease resistance of wild rice, thereby promoting the development of rice blast-resistant breeding work.

The invention discloses bacillus velezensis capable of tolerating various environmental pollution and promoting growth and application of the bacillus velezensis. A plant endophyte bacillus velezensis L381 strain is identified in wild rice, and the strain is high in environmental adaptability, can grow in the environment with the temperature of 15-40 DEG C and the pH value of 5-8, and can tolerate the NaCl concentration of 4%; the rice growth promoting agent has a good growth promoting function, can secrete auxin IAA, solubilize phosphorus, dissolve potassium and secrete siderophores, and can effectively promote rice growth. Meanwhile, the strain has stress resistance to environmental stress such as heavy metals and antibiotics, and tetracycline can be degraded; the strain has a wide antibacterial spectrum, and can be used as a biocontrol bacterium for preventing and treating various plant diseases. The plant endophyte L381 can adapt to variable temperature conditions and variable soil environments in the natural environment, has very strong practical application potential, not only improves the functions of bacillus velezensis, but also widens the application range of the strain.

The invention discloses a cladosporium sp. Fungus YS37 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The cladosporioides sp. Fungus YS37 is a new species separated from the root system of wild rice, is named as Parasarocladium oryzae YS37, is preserved in the China Center for Type Culture Collection (CCTCC), and has a preservation number of CCTCC NO: M 20242286, and the cladosporioides sp. Fungus YS37 is a new species which is separated from the root system of wild rice and is named as Parasarocladium oryzae YS37. The Parasarocladium oryzae YS37 strain can be used for preparing a biological bacterial fertilizer, and when the Parasarocladium oryzae YS37 strain or a fermentation product of the Parasarocladium oryzae YS37 strain is applied to a rice root system, the growth of rice can be effectively promoted, and the plant biomass is increased. The invention provides a new way for application of microorganisms to development of crop production.

The invention discloses an endophytic fungus LH001 and application thereof, and belongs to the technical field of plant disease control. The endophytic fungus LH001 is separated from the root system of wild rice, is classified and named as Pseudophialophora sp.LH001, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M 20241751. The endophytic fungus LH001 strain is co-cultured with rice and colonized at the root of a rice seedling, so that leaf harm caused by magnaporthe oryzae can be reduced, the disease resistance of the rice to leaf blast in the seedling stage is enhanced, and the prevention and treatment effect reaches 94.05%. Therefore, the endophytic fungus LH001 has great value in popularization and application of the biological control effect on the rice blast in the agricultural field.

The invention discloses an application of a oryza longistaminata OlARF9 gene in improving hypoxia stress resistance of rice, and belongs to the field of bioengineering. According to the invention, overexpression is carried out on the oryza longistaminata OlARF9 gene in rice by adopting an overexpression technology, and according to phenotypic changes of transgenic plants, overexpression of the oryza longistaminata OlARF9 gene in the rice can significantly improve the ability of the rice to resist hypoxia stress, so that the oryza longistaminata OlARF9 gene can be applied to improve the resistance of the rice to abiotic stress and improve the resistance of the oryza longistaminata OlARF9 gene in the rice. Comprising the following steps: overexpressing the oryza longistaminata OlARF9 gene in rice to obtain a transgenic plant, wherein the resistance of the obtained transgenic plant to hypoxia stress is obviously enhanced. The method has application prospects in the aspects of improving and enhancing rice stress resistance, accelerating the stress-resistant molecular breeding process and the like.

The invention relates to the technical field of biological genes, in particular to clone of an oryza brachytonkinensis gene SBPL6 and application of the oryza brachytonkinensis gene SBPL6 to improvement of stigma traits of a rice male sterile line, in particular to application of the oryza brachytonkinensis gene SBPL6 to improvement of stigma traits of a sterile line, and improvement of the stigma traits of the sterile line is beneficial to improvement of outcrossing ability of the sterile line. The cDNA (complementary deoxyribonucleic acid) sequence of the gene SBPL6 is shown as SEQ ID NO.2. The wild brachyglossum gene SBPL6 can effectively increase the hair brush length and the stigma length of a rice stigma, so that the stigma exsertion rate is increased, and the outcrossing capability of a sterile line is favorably improved.

The utility model discloses a wild rice shallow soil detecting and sampling device which comprises a soil detecting and sampling mechanism and a soil collecting assembly, the soil collecting assembly comprises a lifting moving block, a through notch is formed in the lifting moving block, a threaded lead screw is installed in the through notch, and the threaded lead screw is connected with the lifting moving block. A threaded screw rod is connected to the interior of a lifting moving block in a penetrating manner, and in the rotating process of a spiral blade shaft, crushed soil can be continuously conveyed into a conveying cylinder and then conveyed into a sampling pipe through a connecting pipe, so that continuous sampling can be realized, and the working efficiency is improved; according to the wild rice shallow soil sampling device, soil can be prevented from being manually taken out and put into a sampling pipe, the wild rice shallow soil sample can be conveniently collected and collected at the same time, the opportunity of manually contacting the wild rice shallow soil sample can be reduced, and the risk that the wild rice shallow soil sample is polluted is reduced.

We offer a furikake (rice seasoning) product that allows you to enjoy the unique flavor of the leaves and stems of the wild rice plant. [Solution] The seasoning product 10 containing Makomo charcoal powder comprises a powdered seasoning 200 and a container body 100 for holding the seasoning. The container body has a storage section 140 for holding the seasoning, a first zipper member 162, and a second zipper member 164, and the storage section can be repeatedly sealed by interlocking the first zipper member and the second zipper member. The seasoning is a powder mixture of Makomo leaf powder, which is made by crushing dried Makomo leaves, Makomo charcoal powder, which is made by crushing carbonized Makomo stems, kelp powder, which is made by crushing dried kelp, salt koji powder, which is made by crushing dried salt koji, and salt powder.

The utility model relates to the technical field of wild rice storage devices, and discloses an ex-situ storage device for oryza meyeriana, which comprises a base, a sliding plate is slidably connected to the inner wall of the base, a spring is fixedly connected to the outer wall of the sliding plate, and a hollow frame is fixedly connected to the outer wall of the spring. The outer wall of the hollow frame is fixedly connected to the inner wall of the base, the outer wall of the sliding plate is slidably connected to the inner wall of the hollow frame, the outer wall of the sliding plate is fixedly connected with a sliding column, the outer wall of the sliding column is slidably connected to the inner wall of the hollow frame, and the outer wall of the sliding column is fixedly connected with a clamping block. And a fixing block is arranged on the outer wall of the clamping block. According to the flowerpot, the sliding plates are pushed to extrude the springs and drive the sliding columns to slide, then the clamping blocks are driven to slide out of the inner walls of the fixing blocks, locking of the fixing blocks is relieved, the flowerpot wall can be detached from the base, a user can conveniently clean rotten or diseased soil, and the effects of facilitating cleaning and maintenance and enhancing plant protection are achieved.

The application discloses an endophytic fungus LH001 and application thereof, and belongs to the technical field of plant disease control. The endophytic fungus LH001 is separated from the root system of wild rice, is classified and named as Pseudophialophora sp. LH001, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M 20241751. The endophytic fungus LH001 strain is co-cultured with rice, is allowed to colonize the root of rice seedlings, can reduce the leaf damage caused by Magnaporthe oryzae, enhances the disease resistance of rice seedlings to leaf blight, and the control effect reaches 94.05%. Therefore, the biological control effect of the endophytic fungus LH001 on rice blast has great value in the popularization and application in the agricultural field.

This invention discloses an InDel marker, Pmct3, for cold tolerance during rice budding, along with its identification method and applications, belonging to the field of molecular marker technology. This invention develops an InDel molecular marker from the insertion / deletion sequence between the Dongxiang wild rice introgression line D2 (background of the cold-tolerant rice 9311) and the cold-sensitive rice 9311 at the Chr1:28249918 and Chr1:28249919 sites in the O. sativa reference genome. Primers are then developed based on this molecular marker for identifying cold tolerance during rice budding. The detection cycle of this invention is only 2-3 hours, does not rely on subjective experience in traditional detection methods, and can obtain accurate and objective detection and identification results in a short time. It is low-cost, high-throughput, and highly specific, making it suitable for rice breeding and production practices.

The invention discloses an InDel marker Pmct1 for the cold-tolerant character of rice in the bud stage as well as an identification method and application of the InDel marker Pmct1, and belongs to the technical field of molecular markers. The InDel molecular marker is developed from insertion and deletion sequences of a Dongxiang wild rice introgression line D2 under the background of a bud-stage strong cold-resistant material 9311 and a bud-stage cold-sensitive material 9311 at an O.sativa reference genome Chr1: 32106831.. 32106871 site, and a primer is developed according to the molecular marker, so that the InDel molecular marker is used for identifying the bud-stage cold-resistant character of rice. The detection period of the method only needs 2-3 h, the method does not depend on subjective experience of people in traditional detection, accurate and objective detection and identification results can be obtained in a short time, and the method is low in cost, high in flux, high in specificity and suitable for rice breeding and production practice.

1. Name of the product in this design: Packaging bag (Fuyuan Wild Red Rice Meal Replacement Powder). 2. Purpose of this design: For packaging and storing rice. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: the front view. 5. This design is for a thin product, therefore the left view, right view, top view, and bottom view are omitted.

The embodiment of the present application provides a method, system and equipment for intelligent assessment of rice blast disease grade and initial screening of disease resistance, the method comprising: S1: collecting image data of wild rice leaves infected with rice blast, performing instance segmentation on the image data by improving the YOLOv8‑Seg segmentation model, and obtaining segmentation results, wherein the improvement measures include: GhostHierarchicalNet backbone network, CAHSFPN neck network and LSDECS Head segmentation head; S2: based on the segmentation results, counting the number of pixels and their proportion of diseased leaves and diseased areas, and combining the disease resistance identification technical regulations to obtain disease grade assessment results; S3: based on the disease grade assessment results repeated multiple times, screening wild rice materials with strong disease resistance. This method can effectively improve the accuracy and efficiency of crop disease assessment, provide support for large-scale wild rice disease resistance screening, and thus promote the development of rice blast resistance breeding.

The utility model relates to the technical field of wild rice planting, in particular to a wild rice seed collecting device which comprises a fixing rod, a plurality of receiving plates used for receiving seeds are arranged above the fixing rod, the receiving plates are combined to form a ring, and a contraction assembly used for folding and unfolding the receiving plates is arranged on the fixing rod. The contraction assembly comprises a plurality of gears, connecting blocks, first connecting rods and second connecting rods. The two ends of each first connecting rod are fixedly connected with the gears adjacent to the first connecting rod correspondingly. The gears are in running fit with the adjacent connecting blocks, and the gears located on the same connecting block are meshed with each other. The fixing rods are fixedly connected with the connecting blocks adjacent to the fixing rods, and a rotating shaft is coaxially and fixedly connected to one gear located above the fixing rods. The gear fixedly connected with the rotating shaft is driven to rotate by rotating the handle, the connecting rod mechanism can be driven to be unfolded or folded, seeds can be collected after being unfolded, the seeds can be stored after being folded, the size is reduced, and transportation and storage are convenient.

The application discloses an indole acetic acid producing endophytic microbacterium and application thereof. Microbcaterium The microbacterium (KlspL18) is separated from leaves of Oryza granulata Ohwi, and is preserved in the China Center for Type Culture Collection, and the number is CCTCC M 2022446. The strain of the application can produce IAA in large quantity, has a strong inhibiting effect on rhizoctonia solani, and can obviously promote seed germination and plant growth of rice after being colonized into the rice. The strain provided by the application has advantages of promoting plant growth, preventing and treating rhizoctonia solani and improving soil fertility.

This invention discloses a new gene, OlGn8.2, derived from wild rice Oryza longistaminata and applications thereof, belonging to the field of crop genetic engineering and molecular biology techniques. The OlGn8.2 was introduced into the near-isogenic line NIL-OlGn8.2 or overexpressed in rice variety 9311, resulting in thicker stems and a significant increase in the number of primary and secondary branches as well as the number of grains per panicle. These results indicate that the OlGn8.2 significantly enhances rice yield by promoting growth and development, increasing the development of the primary and secondary branches, and consequently increasing the number of grains per panicle. Therefore, the OlGn8.2 has great potential for application in the breeding of high-yield rice varieties.

The invention discloses a grain length gene cloned from African wild rice and application of the grain length gene. The invention aims to solve the problem of how to regulate and control agronomic traits of plants. The invention provides an application of a protein or a substance for regulating the expression of a coding gene of the protein or a substance for regulating the activity or content of the protein in regulating the agronomic traits of plants, and the protein is an ObGL1 protein, is a protein with an amino acid sequence of SEQ ID NO: 2, and is obtained by translating the grain length gene. The agronomic traits are grain weight and / or grain length and / or plant height and / or grain number per ear and / or single plant yield, and the regulation agronomic traits can be up-regulated, increased or increased grain weight and / or grain length and / or plant height and / or grain number per ear and / or single plant yield of main stem ears. The invention provides a new germplasm resource for genetic breeding work, provides a new material for rice variety breeding, and plays a positive role in accelerating improvement of rice varieties.

The invention discloses an SNP (Single Nucleotide Polymorphism) site combination for distinguishing wild rice from indica type cultivated rice and application of the SNP site combination. The SNP site combination comprises 180 SNP sites which are uniformly distributed on a rice whole genome. The genotypes of the 180 SNP loci in rice to be detected are detected, if the genotypes of at least 60% of the SNP loci in the rice to be detected are consistent with the genotype of the wild rice, the rice to be detected is identified as the wild rice, and if the genotypes of at least 60% of the SNP loci in the rice to be detected are consistent with the genotype of the indica type cultivated rice, the rice to be detected is identified as the indica type cultivated rice. By utilizing the 180 SNP site combination provided by the invention, wild rice and indica type cultivated rice can be quickly distinguished, so that the wild rice resource can be more sufficiently utilized.

The application relates to a method for creating a fertile tetraploid hybrid between a cultivated rice and an Oryza australiensis genome, which comprises the following steps: using CX35-2X or YZ32-2X as a female parent material, and Oryza australiensis EE as a male parent material to perform hybridization, thereby obtaining first-generation hybrid seedlings; doubling the first-generation hybrid seedlings by colchicine, thereby obtaining hybrid tetraploid seeds. The application utilizes the whole genome transfer and polyploidization, that is, a distant hybrid containing a complete wild rice genome is obtained through distant hybridization, and then a hybrid allopolyploid is obtained through chromosome doubling, so that the excellent genes of the wild rice and the distant hybridization advantage of the polyploid are fully utilized.

The embodiment of the invention provides an intelligent evaluation method and system for the salt tolerance of wild rice based on deep learning, and the method comprises the steps: S1, obtaining the wild rice, collecting the pictures of the wild rice, and making a data set; s2, using the data set to train an improved YOLOv8-seg model, and obtaining a wild rice salt tolerance intelligent evaluation model ST-YOLO, the improvement measures comprising: constructing a C2f-DBB module to replace a convolutional layer in the C2f module, introducing a convolution and attention fusion module CAFM, and using a deformable convolution and spatial information enhancement module to design a spatial pyramid pooling layer; s3, performing instance segmentation on an input image by using the ST-YOLO model, and outputting a green leaf pixel number, a yellow leaf pixel number and a total leaf pixel number of a single plant of wild rice leaf; and S4, obtaining the salt tolerance grade of the wild rice based on the pixel number. According to the method, by improving the YOLOv8-seg model, the feature extraction and fusion capability is improved, automatic salt tolerance grade evaluation is achieved, the precision is high, the real-time performance is high, and germplasm screening is assisted.

The invention relates to the technical field of biology, and particularly provides a diploid wild rice genetic transformation and polygene editing method and system. According to the method, a wild rice genetic transformation platform is established by optimizing conditions of an induction culture medium, agrobacterium infection and a regeneration culture medium; on the basis, key genes are domesticated; for example, a zinc finger transcription factor coding gene PROG1 for controlling the plant type of wild rice to be converted from creeping growth to upright growth, a gene D2 for determining the size of a tillering angle, a transcription factor coding gene An-1 for controlling the awn length and yield of the wild rice, a key pleiotropic gene DEP1 for controlling the yield of the rice, a coding gene GW2 for controlling the grain width and a gene Bh4 for controlling the glume shell color of the wild rice are used as target genes; a CRISPR / Cas9 mediated diploid wild rice polygene editing system is constructed, a gene editing stable plant is obtained through genetic transformation, an annual and perennial diploid wild rice genome editing technical system is established, and then diploid wild rice stress-resistant novel gene resources and new germplasm with breeding utilization value are rapidly obtained.

The present application relates to the technical field of biological genes, and particularly relates to a short-tongue wild rice gene SBPL6 cloning and application thereof in improving stigma traits of a rice male sterile line, in particular, application of the short-tongue wild rice gene SBPL6 in improving stigma traits of the sterile line, and the improved stigma traits of the sterile line help to improve the outcrossing ability of the sterile line, the cDNA sequence of the gene SBPL6 is shown as SEQ ID NO. 2, the short-tongue wild rice gene SBPL6 can effectively increase the stigma brush length and the stigma length of rice, thereby improving the stigma exposure rate and helping to improve the outcrossing ability of the sterile line.

The invention discloses an InDel marker Pmct3 for the cold-tolerant character of rice in the bud stage as well as an identification method and application of the InDel marker Pmct3, and belongs to the technical field of molecular markers. The InDel molecular marker is developed from an insertion and deletion sequence between Chr1: 28249918 and Chr1: 28249919 sites of an O.sativa reference genome of a Dongxiang wild rice introgression line D2 under the background of a bud-stage strong cold-resistant material 9311 and a bud-stage cold-sensitive material 9311, and a primer is developed according to the molecular marker, so that the InDel molecular marker is used for identifying the bud-stage cold-resistant character of rice. The detection period of the method only needs 2-3 h, the method does not depend on subjective experience of people in traditional detection, accurate and objective detection and identification results can be obtained in a short time, and the method is low in cost, high in flux, high in specificity and suitable for rice breeding and production practice.

The invention discloses a breeding method for increasing rice anther and increasing pollen quantity, which comprises the following steps of: identifying a plurality of chromosome single-fragment substitution lines which stably show large anther characters from a single-fragment substitution line (SSSLs) library which takes wild rice as a donor and Huajing indica 74 as a receptor; positioning the anther QTL carried on the substitution fragment of each chromosome single-fragment substitution line by utilizing linkage analysis; chromosome single-segment substitution lines carrying different anther QTLs are hybridized and selfed, molecular marker-assisted selection is combined, a homozygous multi-segment polymerization line is constructed, and the homozygous multi-segment polymerization line comprises at least one of a homozygous two-segment polymerization line, a homozygous three-segment polymerization line, a homozygous four-segment polymerization line and a homozygous five-segment polymerization line. According to the breeding method disclosed by the invention, a batch of multi-fragment polymerization line materials with the characteristics of large anther and large pollen quantity of rice can be obtained, and a new method and germplasm material are provided for improving the seed production yield and efficiency of hybrid rice.

The utility model relates to the technical field of cultivation protection, and discloses a wild rice heterohabitat protection net which comprises an outer layer, connecting pieces are fixedly connected to the left side and the right side of the outer layer respectively, a plurality of fixing rings are rotationally connected to the opposite sides of the two connecting pieces respectively, and fixing pipes are fixedly connected to the inner diameters of the fixing rings respectively. The bottom ends of the multiple fixing pipes are fixedly connected with puncture cones, the tops of the multiple puncture cones are rotationally connected with threaded rods, the surfaces of the multiple threaded rods are in threaded connection with moving parts, and multiple rotating rods are arranged between the multiple moving parts and the adjacent fixing pipes. The rotary knob is rotated to drive the threaded rod at the bottom to rotate, the threaded rod rotates to drive the moving part to move, and the moving part drives the rotating part at the bottom to move when moving, so that when the puncture cone is inserted into the ground, the rotating rod is unfolded to quickly fix the puncture cone.

The invention relates to an ultralow-temperature preservation method for root tips of wild rice, and belongs to the technical field of plant cell engineering. The ultralow-temperature preservation method comprises the following steps: S1, taking robust sterile tissue culture seedlings of wild rice subjected to subculture for 20-60 days, cutting off root tissues, inoculating the seedlings into a sucrose-containing MS culture medium, and inducing new roots; s2, transferring the sterile tissue culture seedlings with new roots into a culture medium containing cane sugar and ABA for pre-culture; and S3, cutting the root tips of the sterile tissue culture seedlings after pre-culture, and carrying out ultralow-temperature preservation. According to the method, the wild rice root tips are successfully preserved at the ultralow temperature for the first time, and the preserved root tips form calluses and can be differentiated into seedlings, so that the growth condition is good. The reference value is realized on the ultra-low temperature preservation of the wild rice and the gramineous plants.

The present invention discloses a fungus of the genus Parasarocladium oryzae Verticicladium sp. YS37 and its application in promoting rice growth, belonging to the field of microbial technology. The fungus Verticicladium sp. YS37 is a new species isolated from the roots of wild rice and named Parasarocladium oryzae YS37, which is deposited in the China Center for Type Culture Collection with the deposit number of CCTCC NO: M 20242286. Parasarocladium oryzae The strain YS37 can be used to prepare bio-fertilizer. Applying the strain YS37 or its fermentation product to the roots of rice can effectively promote rice growth and increase the plant biomass. The present invention provides a new way for the development of microbial application in crop production.

This invention discloses an InDel marker, Pmct1, for cold tolerance during rice budding, along with its identification method and applications, belonging to the field of molecular marker technology. This invention develops an InDel molecular marker by combining the insertion / deletion sequences of the Dongxiang wild rice introgression line D2 (background of the cold-tolerant rice 9311) and the cold-sensitive rice 9311 at the Chr1:32106831..32106871 locus in the O. sativa reference genome. Primers are then developed based on this molecular marker for identifying cold tolerance during rice budding. The detection cycle of this invention is only 2-3 hours, does not rely on subjective experience in traditional detection methods, and can obtain accurate and objective detection and identification results in a short time. It is low-cost, high-throughput, and highly specific, making it suitable for rice breeding and production practices.