108 results about "Oryza rufipogon" patented technology

The invention relates to a method for high-efficiently cultivating excellent later generation of the wild rice distant hybridization , which is to cultivate the wide rice distant-hybridization high-efficient excellent later generation through the hybridization of the cultivated rice, the embryo rescue, the strong seedling culture, the pollen culture and the molecular marker assisted selection of the hybridization later generation of the cultivated rice and the wide rice. The survival rate of the distant hybridization of the invention is high; the hybrid progeny is backcrossed at the preliminary period and then self-bred, thus being beneficial to overcome the reproductive barrier caused by the different genome in the hybrid progeny of the wide rice and the cultivated rice, and to stabilize more genetic characters of the wide rice inside the cultivated rice; the pollen culture can accelerate the stability of the purification of the hybrid progeny; with the established molecular marker assisted selection, the breeding can be accurately performed without being influenced by the environmental conditions and being restricted by the growth and development period.

The present invention discloses a screening method for breeding strong cold-resisting new rice cavity, it uses wile rice as female parent and uses cultured rice as male parent, and adopts the following steps: making sexual hybridization to obtain hybrid, planting to obtain seed, culturing said seed to grow two-leaves and one-heart, and making treatment for 48-72 hr. in refrigerator or biological culture box of -2- +1 deg.C, restoring for 5-10 days at normal temp. in the room, transplanting living seedling into field, after it is grown up and ripened, hibernating and screening at low temp. -5-+1 deg.C, using hibernated plant with regeneration capacity to make multiple hybridization or backcross, making its progeny undergo the indoor and outdoor low-temp. treatment and screen at -5 - +1 deg.C, making family selectino and obtaining said new variety.

A method for selectively culturing the irregular allopolyploid rice includes such steps as choosing parents, hybridizing while treating with hormone, embryonic retrieval culturing, reduplicating chromosome, reduplicating calli or fasciculate embryos, growing to become seedlings, rooting culture, transplanting, culturing and discriminating. The resultant rice may be irregular allohexaploid rice or irregular allooctaploid rice.

The present invention provides polyploid rice selectively breeding and identifying method with polyploid meiosis stability (PMeS) gene. The rice selectively breeding process includes: selecting parents; hybridization or complex hybridization; back crossing; doubling chromosome; identifying polyploid; selecting bearing property; observing meiosis behavior; self-crossing to form stable line; and identifying high bearing property. The identifying indexes include: polyploid line of Asia cultivated indica type rice or japonica rice; bearing rate not lower than 65 %; normal meiosis behavior; normal pollen shape and physiological activity and normal dyed pollution with 0.1 5 I-KI not lower than 95 %; bearing rate of hybrid of PMeS polyploid rice and other polyploid rice variety over 70 % and that of PMeS polyploid rice and wild rice variety over 65 %. The present invention solves the problem of low bearing rate of polyploid rice and may be used in breeding polyploid rice variety.

The invention discloses a method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue, belongs to the field of breeding by the biological technique, and aims at reducing the alcohol disinfecting time and reducing the alcohol concentration as well as creating foundation for the survival rate of hybridization seedlings. The method is that the MS of an embryo culture is improved; the concentration of KNO3, NH4NO3, KH2PO4 and MnSO4.4H2O is reduced; meanwhile, CuSO4.5H2O, CoCl2.6H2O, niacin, pyridoxine hydrochloride and thiamine hydrochloride are not added; the hardening-seedling technology is innovated; when in hardening seedling of the plants, the hardening-seedling treatment with convenient operation and good effect is carried out, so that the survival rate of the seedlings can be greatly increased. According to the operation of the method, the operation of embryo rescue is simplified; the whole culture costs 30 days only, so that the culture cycle can be greatly reduced; meanwhile, the seedling rate can be greatly increased and up to 94%; therefore, the breeding efficiency of the wild rice and cultivated rice distant hybridization embryo rescue can be increased.

The invention belongs to the technical field of cross breeding of plants and relates to a novel method for improving an achievement rate of a first backcross hybrid generation of oryza sativa and oryza minuta. The method includes that firstly, the oryza sativa is used as a female parent, the oryza minuta is used as a male parent, and the oryza sativa and the oryza minuta are subjected to hybridization and embryo rescue to obtain an interspecific hybrid F1 plant; secondly, the interspecific hybrid F1 plant is used as a female parent, the corresponding oryza sativa is used as a male parent, the interspecific hybrid F1 plant and the corresponding oryza sativa are subjected to backcross, by means of a method which is similar to a method of hybrid rice seed production, and the pollination is directly performed for 3 consecutive days to obtain a plurality of young embryos of a first wide cross and backcross hybrid generation; and finally, the plurality of young embryos are subjected to the embryo rescue to obtain the first backcross hybrid generation BC1F1 plant. According to the novel method for improving the achievement rate of the first backcross hybrid generation of the oryza sativa and the oryza minuta, the method is simple in operating and low in cost, the achievement rate can be stably improved from current 0.02% -0.03% to 0.32%-0.35%, and the problem that the first backcross hybrid generation BC1F1 of the oryza sativa and the oryza minuta is difficult to achieve is solved.

The invention discloses a QTL-seq based method for mining a cold-tolerant gene of Dongxiang wild rice. According to the method, cultivated rice 'Xieqing Zao B' is taken as a receptor parent, namely, a female parent; the Dongxiang wild rice is taken as a male parent; a recombinant inbred line BC1F10 obtained by distant hybridization and continuous backcrossing is subjected to cold tolerance identification; obtained 20 cold-tolerant strains form a cold-tolerant gene pool; and 20 cold-sensitive strains form a cold-sensitive gene pool. The two gene pools are subjected to high-throughput sequencing and QTL-seq analysis to obtain differential SNP makers and cold-tolerant character related QTL loci of the two gene pools; candidate cold-tolerant genes are subjected to fluorescent quantitative PCR detection; and finally the Dongxiang wild rice is detected to contain five cold-tolerant related genes by identification. According to the method, the cold-tolerant genes of the Dongxiang wild rice can be quickly and accurately detected and mined, a powerful evidence can be provided for comprehensively illuminating a genetic mechanism of cold tolerance character of the Dongxiang wild rice, a foundation is laid for cloning and functional analysis of the cold-tolerant genes of the Dongxiang wild rice, and the breeding practice of the cold-tolerant genes of the Dongxiang wild rice is facilitated.

The invention relates to a method for identifying viability of perennial rice naturally wintering rice stump, in particular to a method for breeding highly cold-resistant rice materials. The method is characterized in that: via performing sexual hybridization and backcross to cultivated rice and highly cold-resistant Dongxiang wild rice, then the later remained stump can live through the winter under a natural condition; in the next spring, the rice stump is dormant bud and germinates into seedlings, and the new perennial rice variety is obtained through 6 to 8 generations. The new perennial rice variety grows maturely and is harvested; the remained stump can live through the winter under the natural condition; the viability of the rice stump is identified after a clod winter (extremely low temperature at minus 12 DEG C); the identifying method relates to visual observation and touching identification. The method has the characteristics of convenience, fastness, easy control, excellent effect and the like. The new rice variety identified by the method is highly cold-resistant and is capable of wintering naturally. Breeding the perennial rice stump has great significance in the development of food production.

The invention discloses a method for quickly cultivating a novel cytoplasm male sterility line of rice. The method comprises the following steps of: using dongxiang wide rice as a female parent and a variety or a strain which is good in column exposure, excellent in comprehensive character and good in combining ability as a male parent to hybridize to obtain F1; and then, performing backcrossing by using the varieties and strains as the backcrossing male parents; and from second backcrossing generate, selecting a single plant with pollen sterility phenotype similar to that of the backcrossing male parent to perform backcrossing with the backcrossing male parent, and continuously backcrossing for 5-6 times to obtain the male sterility line with dongxiang wide rice as an aborted cytoplasm source. The backcrossing male parent variety or strain is a maintainer line of the male sterility line. The novel cytoplasm male sterility line of rice can be cultivated in an accelerated manner by the technical method provided by the method disclosed by the invention, and the method is good in repeatability and reliable in result.

The invention discloses a method for protection and breeding of medicinal wild rice and belongs to the technical field of plant tissue culture. The method for protection and breeding of medicinal wildrice comprises steps as follows: explant selection, seed treatment, seed sterilization, seed germination acceleration, seed bud callus induction, callus material treatment, callus induction culture,callus subculture, differentiation culture, primary subculture differentiation culture, secondary subculture differentiation culture, differentiation multiplication culture, strong seedling culture, rooting culture, seedling hardening culture, greenhouse transplantation of seedlings, field transplantation of the seedlings and field seedling management. The problem of difficulty in seed callus induction and seedling differentiation of the wild rice is solved, the callus rate and the differentiation rate are higher through induction, and medicinal wild rice seedlings obtained through tissue culture and rapid propagation grow well. The method provides powerful technical support for seedling differentiation of the medicinal wild rice and other rice varieties with difficulty in tissue culture through tissue culture and rapid propagation of seeds and also provides an effective method for protection and utilization of the medicinal wild rice.

The invention provides a method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction. The method includes the following steps: a, disposing wild rice under dark conditions to enable the flowering stage of the wild rice and the flowering stage of cultivated rice to converge; b, hybridizing the cultivated rice with the wild rice; c, after the hybridization, spraying young embryo development promoting fluid; d, carrying out embryo rescue cultivation; e, hybrid embryos send forth sprouts or calluses; f, treating the sprouts or the calluses with colchicines; i, transplanting tissue culture seedlings; j, selecting heterogenous hybrid seedlings; and k, determining allotetraploid rice or allohexaploid rice. Through the method, a plurality of species of heterogeneous hybrids and allopolyploids among a plurality of genomes can be obtained, and the method develops a new path for crossbreeding of cultivated rice / wild rice and full utilization of favorable genes of the wild rice.

The invention provides a Dongxiang wild rice endophytic fungus strain capable of transforming glycyrrhizic acid to produce glycyrrhetinic acid monoglucuronide. The strain is identified and named as Aspergillus flavus DX-SEL2 and preserved in China Center for Type Culture Collection, with an accession number of CCTCC NO: M2013686. The strain can induce generation of beta-glucuronidase in a transformation medium with a glycyrrhizic acid as an inductive agent, then hydrolysis is carried out so as to remove a glucuronyl group at the terminal of a glycyrrhizic acid molecule, so glycyrrhetinic acid monoglucuronide is produced (secreted in fermentation broth). Organic solvent extraction, normal-phase silica gel column chromatography, reversed-phase silica gel column chromatography and the like are used for further separation and purification so as to obtain glycyrrhetinic acid monoglucuronide. According to the invention, microbial catalytic conversion is employed, and the advantages of greenness, environmental protection, no pollution, simple transformation steps, a single product, a high transformation rate, etc. are obtained.

A method for ultralow-temperature storage and renewal cultivation of wild rice stem tips includes steps: taking a subcultured sterile tissue culture seedling, keeping the base portion, culturing in a culture medium free of hormone MS, cutting new sprouts of the base portion, transferring to a culture medium containing 0.3molL<-1> of sucrose and 0.5-5mgL<-1> of abscisic acid to realize culturing, transferring to a culture medium containing 0.7molL<-1> of sucrose and 0.5-5mgL<-1> of abscisic acid to realize preculture, stripping the stem tip, pre-culturing in an MS solid culture medium containing 0.4molL<-1> of sucrose and 2molL<-1> of glycerin, gently dipping in 2-4% calcium-free sodium alginate solution, putting on a loading strip, immersing in an MS liquid culture medium containing 0.1-0.3% of calcium chloride, 0.4molL<-1> of sucrose and 2molL<-1> of glycerin, immobilizing, sucking excessive liquid, treating in a pretreatment solution, transferring to a vitrification protective agent PVS2 to realize ice-bath treatment, and putting into liquid nitrogen to realize storage.The method has the advantages of simplicity, convenience and feasibility in storage, stability, reliability and favorable recovery growth conditions of stored wild rice.

The invention discloses a promoter of a gene Me094 related to bacterial-blight resistance of Oryza meyeriana. The promoter belongs to tissue specificity expression promoters and can drive specific expression of a GUS gene in leaves of transgenic Arabidopsis. The promoter can be applied to seed breeding of rice resistant to bacterial blight, and can improve resistance of the rice to bacterial blight by driving concentrated specific expression of other exogenous genes especially genes resistant to bacterial blight in leaves of the transgenic rice. Additionally, the promoter has great theoretical and practical significance in prevention of other foliage diseases of the rice.

The invention provides a cultivating method of cross-year regrowing rice seeds. According to the cultivating method, wild rice containing cold-resisting and root-flourishing genes is adopted as a male parent, DS89-1 glutinous rice is adopted as a female parent, 3-5 times of recrossing is carried out, and then the cross-year regrowing rice seeds can be obtained. The invention further provides a seedling planting method of the cross-year regrowing rice seeds. Rice which is grown from the cross-year regrowing rice seeds obtained through the cultivating method and is planted according to the seedling planting method has the advantages of being high in yield and milled rice rate, good in rice quality, capable of regrowing in the next year and strong in cold resistance, drought resistance, lodging resistance and Magnaporthe oryzae resistance.

The invention relates to a method, belonging to the plant cross breeding technology, which aims to solve the problem that affinity does not exist in the hybrids between the cultivated rice and the wild rice with various genome types; the invention is disclosed to improve the rate of immature embryos. The invention adopts the technical proposal that by temperature and humidity regulation during heading and flowering stage and the application of wind proofing facilities, a large amount of high-activity fresh pollen of the wild rice with various genome types is acquired; then deep color bagging or hot water treatment is utilized to adjust the flowering time encounter of male parent and female parent; the anther of singular floret is clipped by a nipper, or the spikelet is arranged on the female parent panicle to be pollinated, and then the spikelet is shaken by hand; the pollen utilization ratio of the wild rice with various genome types is improved, and the effect is better after two or three times of repeated pollination. By utilizing the hybridization between various types of cultivated rice material and wild rice with various genome types, materials with good affinity are selected to be hybridized with wild rice with various genome types. The invention has the advantages of simple operation, low cost, and the improvement of the rate of immature embryos from 0 to 40% to 30% to 60%; the method preferably solves the problem that affinity does not exist in the hybrids between the cultivated rice and the wild rice with various genome types, and the rate of immature embryos is greatly improved.

The invention belongs to the technical field of transgenic environmental biology safety detection and analysis, and particularly relates to a detecting and evaluating method for the environmental risk caused by the transgene escape of herbicide-resistant rice. The detecting and evaluating method comprises the following steps: using an artificially modified EPSP synthetase herbicide-resistant transgenic rice, a non-transgenic rice and a wild rice relative population as analysis materials, performing artificial hybridization, establishing a hybrid F1 population comprising a group containing epsps transgenes and a group no containing the epsps transgenes as well as an F2-separated generation experimental population comprising a group containing the epsps transgenes and a group no containing the epsps transgenes; the planting the experimental population in different modes in the environment with glyphosate and the environment without glyphosate, and measuring five relative fitness traits. According to the method, the environmental risk to the wild relatives, caused by the transgene escape of the herbicide-resistant rice, is detected through calculating the complex fitness index of the transgene to the populations. The detecting result is accurate, the efficiency is high, and the method has a favorable application prospect in the aspect of the environment safety assurance in the herbicide resistant transgenic rice production process.

The invention provides a method for identifying hypoxia tolerance and flooding tolerance of rice. The method comprises the following steps: performing sexual hybridization and backcrossing on cultivated rice and Dongxiang wild rice with good hypoxia tolerance and flooding tolerance, selecting a signal plant material from separated descendants of the hybrid rice, putting full dried seeds into a water container with water of 20-26cm deep, putting the water container into an incubator of 25-30 DEG C, detecting water absorption, germination, budding and seedling emergency of seeds, enabling the rice growing out of the water level to grow normally, breeding for 5-6 generations, thereby breeding a novel variety of rice with good hypoxia tolerance and flooding tolerance. The method for identifying hypoxia tolerance and flooding tolerance of rice, which is provided by the invention, has the characteristics of being simple, convenient, accurate, easy to handle, and the like; the novel variety of rice identified by using the method is good in hypoxia tolerance and flooding tolerance, a novel direct seeding rice variety can be bred, and the method has great meanings of cost reduction, synergism and production and yield increase in grain production.

The invention discloses a method for cloning a piricula oryzae gene. The method comprises the following steps of: 1) designing a primer according to a known piricula oryzae gene so as to obtain a primer pair; 2) performing polymerase chain reaction (PCR) amplification on the primer pair obtained in the step 1) by taking pyricularia grisea high-resistant paddy as a template so as to obtain PCR amplification products; 3) sequencing the PCR amplification products obtained in the step 2) so as to obtain the piricula oryzae gene; and 4) transferring the piricula oryzae gene into rice blast susceptible paddy, measuring a resistance spectrum of rice blast to obtain the piricula oryzae gene with a new resistance spectrum, namely the target piricula oryzae gene. Experiments of the method prove that: by the method, the piricula oryzae gene in wild paddy is directly separated and paddy varieties are transformed so as to obtain piricula oryzae paddy; and the resistance spectrum of transgenic plants is determined, and the piricula oryzae gene with a new resistance spectrum is screened out, so that the overall improvement on the paddy varieties is facilitated.

The invention discloses a method for identifying a rice blast-resistant gene of wild rice. The method comprises the steps of cutting middle parts of leaves of wild rice plants, and placing in culture dishes with single-layer filter paper; adding an appropriate amount of sterilized water to each dish; dropping 5mu L spore suspension on each leaf; inoculating each leaf for 5 points; covering lids; placing the culture dishes in an illumination incubator at 23 DEG C for dark culture for 24 hours; carrying out illumination culture with illumination intensity of 3400Lux / 1310Lux (12 hours / 12 hours); and investigating disease states after culturing for 6-7 days, wherein an investigation disease is divided into five levels, 0-2 level is disease-resistant, and 3-4 level is infected. According to the method, a method for inoculating rice blast is optimized; the simpler and large-scale method for identifying the resistance of the wild rice is founded; and the problem that the disease resistance is difficult to identify due to the fact that the wild rice is high in sterility rate, grains fall easily, seeds are difficult to collect, the degree of dormancy is different, and germination growth is inconsistent is solved.

The invention relates to a cutting seedling-raising method capable of making oryza plants ward off diseases and detoxified rapidly and belongs to the technical field of plant detoxification. The cutting seedling-raising method capable of making the oryza plants ward off diseases and detoxified rapidly comprises the steps of material taking form fields, disease warding-off, detoxification and sterilization treatment conducted on stems, stem cuttage preprocessing, stem cuttage and stem rejuvenation and cultivation. By utilizing a plant disease warding-off and detoxification technology, the pollution problem in the oryza stem cuttage process is solved effectively, the stems are higher in survival rate after cuttage, cutting seedlings grow vigorously and are good in growth vigor, and an effective means is provided for rejuvenation and rapid propagation of important hybridization materials and saving of wild rice high-quality germplasm resources. The method is simple, easy to operate and low in cost, the oryza plants surviving through cuttage are good in growth vigor, and diseases and insect pests do not occur basically. In addition, a cutting seedling-raising period is short, next transplantation can be performed in 1-2 months generally and is not affected by seasons and other environmental factors, and large-scale popularization and application can be achieved. The method can be also applied to saving and rapid propagation of certain rare oryza varieties.

The invention relates to the technical field of rice breeding, and specifically relates to a rice mitochondria sterility gene and the application thereof. The invention discloses a rice mitochondria sterility gene and a coding sequence thereof, and D1 type cytoplasm can be quickly and efficiently screened and authenticated from wild rice for cultivation of a D1 type cytoplasm sterile line by designing a specific molecular marker through the gene sequence. A mitochondrial genome specific sequence of the D1 type cytoplasm sterile is authenticated through comparative genomics, sterility related ORFs are authenticated through gene predication and differential expression analysis, and a plant transformation vecter is constructed for transforming maintenance line verification and sterility function.

The invention relates to a method for cultivating a fine breed of hybrid rice by utilizing wild rice. The method includes the specific steps of: first, using a castrated IR58025B as a female parent and hybridizing the female parent with a wild rice to obtain an F1; then using a castrated IR58025B as a female parent and hybridizing the female parent with F1 to obtain an F2; then using the same method for hybrid to obtain an F3; then conducting germination on the F3 grains, using solar energy to carry out focus radiation on the bottoms of the grain sprouts, and breeding a radiation generation 1 seed-grain; selecting dominant plants from the radiation generation 1, and conducting continuous breeding to obtain a radiation generation 4 crossbred wild B, and selecting 35 plants; and carrying out 35 groups of paring hybrid by using IR58025A and the crossbred wild B, selecting close pairing in an A*B growth period, and mainly selecting B line plants with large column, strong vigor and early flowering; then respectively sowing the 35 groups of paring hybrid seeds, planting by germ lines, and conducting microscopic examination to find out 3 groups of aborted sterile germ lines; and finally carrying out separate nursery protection and nursery selection at the same time, and separately collecting and planting the seeds by groups, so as to finally obtain the strong dominant hybridized combination.

The invention discloses a wild rice flower medicine culture one-time seedling development simple culture medium. The culture medium comprises potatoes, KNO3, (NH4)3SO4, Ca(NO3)2, KH2PO4, MgSO4 7H2O, ferric salt, wild rice flower medicine, 2.4-D, NAA,IAA,6-BA, saccharose and agar powder. The potatoes are used for replacing some chemical reagents, the wild rice culture cost is lowered, and storage and reproduction of wild rice resources of a basic agricultural department are facilitated. The culture medium can short the anther culture seedling period which originally needs two-time conversion of a medium to complete seedling, and the anther culture efficiency is improved. The using efficiency of agricultural products in anther culture can be effectively improved, the anther culture technical program is creatively upgraded, and more than half of the technical process and the laboratorial space are simplified.

The invention discloses a rice gene OsPGSIP1. The rice gene OsPGSIP1 has a nucleotide sequence represented by SEQ ID NO.1, or has a coding sequence represented by SEQ ID NO.2; or the rice gene OsPGSIP1 has an amino acid sequence represented by SEQ ID NO.4. The deficiencies in the prior art are overcome, the rice OsPGSIP1 gene is overexpressed in rice Zhenshan 97B, the OsPGSIP1expression level of atransgenic plant increase, and the thousand kernel weight, the seedling stage biomass, the cellulose content and the hemicellulose content increase; when the expression of the OsPGSIP1 gene in the Zhenshan 97B is suppressed, the expression level of OsPGSIP1 in the transgenic plant decrease, and the chlorophyll content and the nitrogen content in leaves increase; and introduction of alleles of OsPGSIP1 derived from wild rice ACC10 into the Zhenshan 97B can significantly increase the seedling stage biomass, the thousand kernel weight, the cellulose content, the hemicellulose content and the stem mechanical strength of the Zhenshan 97B.

The invention relates to a new technical field of rice breeding and particularly relates to a light-sensation two-line cultivation method. The cultivation method concretely comprises the steps of: hybridizing wild rice with commonly cultivated rice, selecting a good strain line, cultivating seeds of the good strain line to germinate, mutagenizing the strain line by focusing sunlight radiation, breeding the multagenized strain line in a iteration generation way, finding a semi-sterile strain in the third generation, cutting stump and tillering for dividing the strain into two strains, respectively transplanting the two strains to places with different elevations, and breeding out light-sensation two lines by the obtained material seeds, wherein the strain in a place in which the daytime is longer than 13 hours is taken as males with fertility and the strain in a place in which the daytime is shorter than 13 hours is mail without the fertility. The light-sensation two-line is bred out by implementing the method, i.e., a new product can be found from variation and stability can be achieved rapidly.

The invention discloses a method for producing low-protein rice, which includes hybridizing wild rice with cultivated rice, back-crossing the wild rice with the cultivated rice prior to multi-generation selfing, using molecular marking assisted selection technology and the like, and screening to obtain a cultivar which is applicable to large-area production, high in yield and low in protein content. The protein content of the rice produced by the method can be reduced evidently, the rice is suitable for patients with kidney diseases to eat, economic burden can be lowered for the patients, and palatability is improved and food therapeutic care is achieved.

The invention discloses a construction method of a wild rice chromsome segment substitution line (CSSL). According to the construction method of the wild rice chromsome segment substitution line, a substitution line population capable of covering an entire chromosome genome is constructed, genetic backgrounds of substitution lines are very clear, for one substitution line, the genetic background of the substitution line and a receptor parent are approximately the same, and a difference with a donor parent exists only in one known marker segment; for a complete set of substitution line, all wild rice chromsome segments have corresponding marking segments, and duplications and deletions are avoided. The constructed wild rice chromsome segment substitution line provides a basis for building a platform for comprehensively conducting wild rice genomics research and long-term beneficial help is provided for rice genomics research and innovation germplasm resources.