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Wild rice flower medicine culture one-time seedling development simple culture medium

A culture medium and wild rice technology, which is applied in the field of wild rice breeding, can solve the problems of loss of good genes in common wild rice, and achieve the effects of improving anther culture efficiency, convenient preservation and reproduction, and efficiency of submission and use

Inactive Publication Date: 2016-02-03
广西壮族自治区农业科学院水稻研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in the course of evolution, many good genes of common wild rice were lost

Method used

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  • Wild rice flower medicine culture one-time seedling development simple culture medium
  • Wild rice flower medicine culture one-time seedling development simple culture medium
  • Wild rice flower medicine culture one-time seedling development simple culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The wild rice anther of the present invention is cultivated once to become seedling simple medium, and in described medium, every 1000ml distilled water contains the raw material of following formula:

[0025]

[0026]

[0027] The pH of the medium was 5.8.

[0028] The wild rice anthers are crushed and added to the culture medium.

[0029] The iron salt is FeSO 4 .7H 2 O and Na-EDTA, FeSO 4 .7H 2 Dissolve O5.57g and Na-EDTA7.45g in 1000ml of distilled water, draw 5.5ml per 1000ml of medium. The potatoes are boiled with water and then filtered to obtain the juice. The weight-to-volume ratio of the potatoes to water is 1:2 mg / ml.

Embodiment 2

[0031] The wild rice anther of the present invention is cultivated once to become seedling simple medium, and in described medium, every 1000ml distilled water contains the raw material of following formula:

[0032]

[0033]

[0034] The pH of the medium was 5.8.

[0035] The wild rice anthers are crushed and added to the culture medium.

[0036] The iron salt is FeSO 4 .7H 2 O and Na-EDTA, FeSO 4 .7H 2 Dissolve O5.57g and Na-EDTA7.45g in 1000ml of distilled water, draw 5.5ml per 1000ml of medium. The potatoes are boiled with water and then filtered to obtain the juice. The weight-to-volume ratio of the potatoes to water is 1:2 mg / ml.

Embodiment 3

[0038] The wild rice anther of the present invention is cultivated once to become seedling simple medium, and in described medium, every 1000ml distilled water contains the raw material of following formula:

[0039]

[0040]

[0041] The pH of the medium was 5.8.

[0042] The wild rice anthers are crushed and added to the culture medium.

[0043] The iron salt is FeSO 4 .7H 2 O and Na-EDTA, FeSO 4 .7H 2 Dissolve O5.57g and Na-EDTA7.45g in 1000ml of distilled water, draw 5.5ml per 1000ml of medium. The potatoes are boiled with water and then filtered to obtain the juice. The weight-to-volume ratio of the potatoes to water is 1:2 mg / ml.

[0044] The medium formula of the present invention has undergone more than 50 inoculation experiments, inoculated more than 15,790 anthers of common wild rice, and the average green seedling differentiation rate reaches 13.4%, which is 5-6.3% higher than the green seedling differentiation rate of the control medium ms and m6 on aver...

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Abstract

The invention discloses a wild rice flower medicine culture one-time seedling development simple culture medium. The culture medium comprises potatoes, KNO3, (NH4)3SO4, Ca(NO3)2, KH2PO4, MgSO4 7H2O, ferric salt, wild rice flower medicine, 2.4-D, NAA,IAA,6-BA, saccharose and agar powder. The potatoes are used for replacing some chemical reagents, the wild rice culture cost is lowered, and storage and reproduction of wild rice resources of a basic agricultural department are facilitated. The culture medium can short the anther culture seedling period which originally needs two-time conversion of a medium to complete seedling, and the anther culture efficiency is improved. The using efficiency of agricultural products in anther culture can be effectively improved, the anther culture technical program is creatively upgraded, and more than half of the technical process and the laboratorial space are simplified.

Description

technical field [0001] The invention relates to the technical field of wild rice breeding, in particular to a simple medium for culturing wild rice anthers into seedlings once. Background technique [0002] Common wild rice is the close ancestor of cultivated rice. Common wild rice has evolved over the years to become modern cultivated rice. But during the evolution process, many good genes of common wild rice were lost. [0003] Wild rice is a perennial aquatic herb. The culm is about 1.5 meters high, and the lower part is spongy or rooted on the nodes. Leaf sheaths cylindrical, loose, glabrous; ligule up to 17mm; auricles conspicuous; leaf blade linear, flat, up to 40cm long, about 1cm wide, margins and midrib scabrous, apex pointed. Panicles about 20 cm long, erect and then drooping; main axis and branches rough; spikelets 8-9 mm long, 2-2.5(3) mm wide, with 2 tiny semicircular degenerated glumes at the base; spontaneous after maturity Detached on spikelet joint; fir...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈成斌赖群珍梁云涛张烨梁世春潘英华
Owner 广西壮族自治区农业科学院水稻研究所
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