Method for quickly cultivating novel cytoplasm male sterility line of rice

Abstract

The invention discloses a method for quickly cultivating a novel cytoplasm male sterility line of rice. The method comprises the following steps of: using dongxiang wide rice as a female parent and a variety or a strain which is good in column exposure, excellent in comprehensive character and good in combining ability as a male parent to hybridize to obtain F1; and then, performing backcrossing by using the varieties and strains as the backcrossing male parents; and from second backcrossing generate, selecting a single plant with pollen sterility phenotype similar to that of the backcrossing male parent to perform backcrossing with the backcrossing male parent, and continuously backcrossing for 5-6 times to obtain the male sterility line with dongxiang wide rice as an aborted cytoplasm source. The backcrossing male parent variety or strain is a maintainer line of the male sterility line. The novel cytoplasm male sterility line of rice can be cultivated in an accelerated manner by the technical method provided by the method disclosed by the invention, and the method is good in repeatability and reliable in result.

Description

technical field [0001] The invention belongs to the technical field of hybrid rice breeding, and in particular relates to a method for rapidly cultivating a novel cytoplasmic male sterile line of rice. Background technique [0002] Since the 1970s, a variety of rice cytoplasmic male sterile lines (CMS) from different sources have been bred by interspecific hybridization and nuclear replacement. At present, rice cytoplasmic male sterile lines from different sources used in production can be generally divided into wild-type (WA-CMS), Baotai-type (BT-CMS) and Honglian-type (HL- CMS) and other three major male sterile cytoplasmic types. [0003] Continuously cultivating cytoplasmic male sterile lines with new male sterile cytoplasmic sources and restorer lines with restorer gene sources is an effective technical means to avoid the potential risks of hybrid rice due to the long-term promotion of a single cytoplasmic source, and it is also an effective technical means to overcome...

AI Technical Summary

Problems solved by technology

However, in the process of cultivating new cytoplasmic male sterile lines, it is often encountered that restorer lines or restorer sources cannot be screened through extensive testing and recovery methods.

For example, the International Rice Institute uses perennial wild rice (female parent) to cross and backcross cultivated rice (hybrid and backcross male parent) to breed a new cytoplasmic male sterile line from perennial wild rice cytoplasmic male sterile source IR66707A, Jiaxing Academy of Agricultural Sciences in my country used this male sterile line as the female parent to breed Jiazao 935A, a new type of male sterile line with good recovery. Restoration lines with strong advantages have not been bred

Images