Detecting and evaluating method for environmental risk caused by transgene escape of herbicide-resistant rice

An environmental risk and herbicide-resistant technology, applied in the field of environmental biosafety detection and analysis

Inactive Publication Date: 2014-02-12
FUDAN UNIV
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  • Description
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  • Application Information

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Problems solved by technology

However, whether the escape of herbicide-resistant transgenes into wild relatives of cro

Method used

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  • Detecting and evaluating method for environmental risk caused by transgene escape of herbicide-resistant rice
  • Detecting and evaluating method for environmental risk caused by transgene escape of herbicide-resistant rice
  • Detecting and evaluating method for environmental risk caused by transgene escape of herbicide-resistant rice

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Embodiment 1

[0046] Utilize the method of the present invention to detect the herbicide-resistant transgenic rice epsps Environmental risks of transgene escape into weedy rice populations. The experimental materials are shown in Table 1.

[0047] The steps for constructing the hybrid experimental groups of wild relatives containing transgenes and non-transgenic wild relatives for environmental biosafety testing are as follows:

[0048] (1) Determine the hybrid combination:

[0049] A. Herbicide-resistant transgenic rice (G1) × weedy rice (WD1),

[0050] B. Non-transgenic rice (P1) × weedy rice (WD1);

[0051] (2) Hybrids F containing herbicide-resistant transgenes (hybrid combination A: G1 × WD1) and non-herbicide-resistant transgenes (hybrid combination B: P1 × WD1) were obtained by artificial crossing 1 group;

[0052] (3) Hybrid F to combination A (G1 × WD1) 1 Molecular testing of populations for bagging self-breeding and herbicide-resistant transgenes identified F 2 Whether indi...

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Abstract

The invention belongs to the technical field of transgenic environmental biology safety detection and analysis, and particularly relates to a detecting and evaluating method for the environmental risk caused by the transgene escape of herbicide-resistant rice. The detecting and evaluating method comprises the following steps: using an artificially modified EPSP synthetase herbicide-resistant transgenic rice, a non-transgenic rice and a wild rice relative population as analysis materials, performing artificial hybridization, establishing a hybrid F1 population comprising a group containing epsps transgenes and a group no containing the epsps transgenes as well as an F2-separated generation experimental population comprising a group containing the epsps transgenes and a group no containing the epsps transgenes; the planting the experimental population in different modes in the environment with glyphosate and the environment without glyphosate, and measuring five relative fitness traits. According to the method, the environmental risk to the wild relatives, caused by the transgene escape of the herbicide-resistant rice, is detected through calculating the complex fitness index of the transgene to the populations. The detecting result is accurate, the efficiency is high, and the method has a favorable application prospect in the aspect of the environment safety assurance in the herbicide resistant transgenic rice production process.

Description

technical field [0001] The invention belongs to the technical field of environmental biosafety detection and analysis, and specifically relates to rice herbicide-resistant transgene ( epsps ) detection and evaluation method of environmental risk caused by escape. Background technique [0002] The rapid development of transgenic technology has greatly promoted the cultivation and planting of genetically modified plants, and the commercial application of genetically modified crops has brought new opportunities for increasing crop yields, reducing the use of pesticides, improving the agricultural ecological environment and food security issues. However, a huge bottleneck facing the commercial planting of transgenic crops is the detection and evaluation of environmental biosafety, especially the escape of crop transgenes and the possible environmental risks, that is, transgenes escape to wild relatives of crops through gene drift , may bring serious weed problems and a series o...

Claims

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Application Information

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IPC IPC(8): A01H1/02C12Q1/68A01H1/04
Inventor 卢宝荣蔡星星汪魏杨箫
Owner FUDAN UNIV
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