Method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue

A technology of distant hybridization and wild rice, which is applied in the field of biological technology breeding, can solve the problems of hybrids not fruiting efficiency, etc., and achieve the effects of shortening alcohol disinfection time, lowering alcohol concentration, and convenient operation

Inactive Publication Date: 2015-09-02
云南省农业科学院生物技术与种质资源研究所
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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of non-fertilization of hybrids and low efficiency in the process of embryo rescue in the process of distant

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  • Method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue
  • Method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue

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Embodiment Construction

[0021] The method of the present invention will be further described below in conjunction with the specific implementation example of interspecific distant hybridization between Jinghong common wild rice (Oryza rufipogon Griff.) and japonica rice 02428. No special instructions in each embodiment are conventional methods.

[0022] Jinghong common wild rice (Oryza rufipogon Griff.) was directly sourced and originally sourced from Yunnan Province, China, and the original source time and direct source time were both in April 2006 (acquisition method). Japonica rice 02428 is commercially available.

[0023] (1) Disinfection treatment of immature embryos

[0024] After 10 days of cross-pollination between common wild rice and japonica rice 02428 in Jinghong, the hybrid spikelets were removed from the female parent japonica rice 02428, and a total of 50 seeds were obtained. Take it back to the laboratory immediately, peel off the chaff and treat it with 65% alcohol by volume fracti...

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Abstract

The invention discloses a method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue, belongs to the field of breeding by the biological technique, and aims at reducing the alcohol disinfecting time and reducing the alcohol concentration as well as creating foundation for the survival rate of hybridization seedlings. The method is that the MS of an embryo culture is improved; the concentration of KNO3, NH4NO3, KH2PO4 and MnSO4.4H2O is reduced; meanwhile, CuSO4.5H2O, CoCl2.6H2O, niacin, pyridoxine hydrochloride and thiamine hydrochloride are not added; the hardening-seedling technology is innovated; when in hardening seedling of the plants, the hardening-seedling treatment with convenient operation and good effect is carried out, so that the survival rate of the seedlings can be greatly increased. According to the operation of the method, the operation of embryo rescue is simplified; the whole culture costs 30 days only, so that the culture cycle can be greatly reduced; meanwhile, the seedling rate can be greatly increased and up to 94%; therefore, the breeding efficiency of the wild rice and cultivated rice distant hybridization embryo rescue can be increased.

Description

technical field [0001] The invention relates to a method for distant hybrid breeding of wild rice and cultivated rice, in particular to a method for improving the efficiency of embryo pull breeding after hybridization of wild rice and cultivated rice, belonging to the field of biological technology breeding. Background technique [0002] Wild rice usually has many excellent genetic characteristics due to its long-term growth in various adversities. It is an excellent genetic resource for improving cultivated rice to increase yield and resist diseases and insect pests, drought, cold, and improve nutrition. Generally, the excellent genetic characteristics of wild rice are integrated into cultivated rice through distant hybridization technology, so as to be used in scientific research and creation of new varieties. [0003] However, the wild rice genome is quite different from the cultivated rice genome. After the cultivated rice with the A genome is crossed with the wild rice ...

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
Inventor 程在全陈玲李定琴余腾琼肖素勤殷富有张敦宇钟巧芳柯学杨雅云付坚陈越王玲仙蒋聪罗红梅李娥贤将春苗曾民赵才美黄兴奇王波
Owner 云南省农业科学院生物技术与种质资源研究所
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