Method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue

Abstract

The invention discloses a method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue, belongs to the field of breeding by the biological technique, and aims at reducing the alcohol disinfecting time and reducing the alcohol concentration as well as creating foundation for the survival rate of hybridization seedlings. The method is that the MS of an embryo culture is improved; the concentration of KNO3, NH4NO3, KH2PO4 and MnSO4.4H2O is reduced; meanwhile, CuSO4.5H2O, CoCl2.6H2O, niacin, pyridoxine hydrochloride and thiamine hydrochloride are not added; the hardening-seedling technology is innovated; when in hardening seedling of the plants, the hardening-seedling treatment with convenient operation and good effect is carried out, so that the survival rate of the seedlings can be greatly increased. According to the operation of the method, the operation of embryo rescue is simplified; the whole culture costs 30 days only, so that the culture cycle can be greatly reduced; meanwhile, the seedling rate can be greatly increased and up to 94%; therefore, the breeding efficiency of the wild rice and cultivated rice distant hybridization embryo rescue can be increased.

Description

technical field [0001] The invention relates to a method for distant hybrid breeding of wild rice and cultivated rice, in particular to a method for improving the efficiency of embryo pull breeding after hybridization of wild rice and cultivated rice, belonging to the field of biological technology breeding. Background technique [0002] Wild rice usually has many excellent genetic characteristics due to its long-term growth in various adversities. It is an excellent genetic resource for improving cultivated rice to increase yield and resist diseases and insect pests, drought, cold, and improve nutrition. Generally, the excellent genetic characteristics of wild rice are integrated into cultivated rice through distant hybridization technology, so as to be used in scientific research and creation of new varieties. [0003] However, the wild rice genome is quite different from the cultivated rice genome. After the cultivated rice with the A genome is crossed with the wild rice ...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of non-fertilization of hybrids and low efficiency in the process of embryo rescue in the process of distant hybridization between cultivated rice and wild rice, and to provide a new method that can quickly and effectively obtain a large number of wild-planted distant hybrid seedlings

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