157 results about "Thiamine hcl" patented technology

Personal care compositions containing an active selected from the group consisting of phlorogine, phlorgine BG, deoxyArbutin, sucrose dilaurate, bakuchiol, pyrenoine, millet, arlatone dioic acid, cinnamic acid, ferulic acid, achromaxyl, methyl nicotinamide, oil soluble licorice extract, folic acid, undecylenic acid, zinc undecylenate, L-tryptophan, thiamine HCl, hexylresorcinol, lipidami red vine, dragosine, methyl gentisate, inositol, symdiol 68, laminaine, their salts, their derivatives, their precursors, and / or combinations thereof. Methods for regulating the condition of mammalian keratinous tissue by topically applying the personal care compositions are also provided.

Personal care compositions containing an active selected from the group consisting of phlorogine, phlorgine BG, deoxyArbutin, sucrose dilaurate, bakuchiol, pyrenoine, millet, arlatone dioic acid, cinnamic acid, ferulic acid, achromaxyl, methyl nicotinamide, oil soluble licorice extract, folic acid, undecylenic acid, zinc undecylenate, L-tryptophan, thiamine HCl, hexylresorcinol, lipidami red vine, dragosine, methyl gentisate, inositol, symdiol 68, laminaine, their salts, their derivatives, their precursors, and / or combinations thereof. Methods for regulating the condition of mammalian keratinous tissue by topically applying the personal care compositions are also provided.

The invention discloses a dietetic food composition for losing weight. The composition comprises the following ingredients: fresh meat and aquatic product, vegetables, fruits, dried mushroom products, cereals, beans, nut meat, edible vegetable oil, eggs, protein hydrolysate powder, dietary fiber powder, konjac powder, guar gum, polyols, phosphatidylcholine, fruit polyphenols, L-carnitine, vitamin A, vitamin E, vitamin C, thiamine hydrochloride, riboflavin, nicotinamide, pyridoxine hydrochloride, folic acid, cyanide-containing cobalt and pantothenic acid. By adopting the dietetic food composition disclosed by the invention, the total energy intake is reduced under the premise of ensuring normal basic metabolic rate, the requirements of the body on protein at a low-energy diet period are improved, and related nutrients are replenished to facilitate body fat, visceral fat and blood lipid metabolism, so as to achieve the target of losing weight. Meanwhile, the invention also discloses a preparation method of the dietetic food composition and use of the dietetic food composition in preparation of a medicament for preventing or treating an obesity disease.

A synergistic composition, or the use of that composition in the manufacture of a medicament, or a method of treatment including the use of that composition, for the treatment of hair loss and baldness, for combined, sequential or simultaneous administration, in any form, via any biological route. In its optimal embodiment the composition consists, in the form of a lotion: 1600-2400 IU / mL Vitamin A Palmitate, 0.64%-0.96% Thiamine Hydrochloride, 0.64%-0.96% Pyridoxine Hydrochloride, 4.8%-7.2% Niacinamide, 2.85%-5.2% D-Panthenol, 1.6%-2.4% L-Arginine, 3.6%-4.4% Methyl Sulphonyl Methane (MSM), 0.08%-0.12% Ginger Oil, 0.08%-0.12% Cinnamon Oil, 0.0996%-0.1494% Oleoresin Capsicum, 1.3%-1.95% Magnesium, 2.4%-3.6% Zinc, 0.192%-0.288% Manganese, 2.6%-3.9% Urea, 2.4%-3.6% Sodium Glycerophosphate, 4.8%-7.2% L-Lysine HCl, plus Preservatives, Co-solvent (Propylene Glycol), Fragrances, Anti-Oxidant, Cooling agent (Menthol), Emulsifier, and Vehicle (Purified water).

The invention relates to a culture medium suitable for a large-scale sympodial bamboo including sinocalamus affinis and Mianzhu and a culture method, in particular to a large-scale sympodial bamboo callus induction and plant regeneration high efficiency culture medium and a culture method thereof. The culture medium comprises an MS (Murashige and Skoog) culture medium and a callus induction and plant regeneration high efficiency culture medium and is characterized in that the MS culture medium is prepared from the following raw materials: (a) macroelements: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate and calcium chloride; (b) microelements: potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulfate and cobalt chloride; (c) organic components: inosite, glycine, aneurin hydrochloride, puridoxinehydrochloride and niacin; and (d), ferric salts: disodiumedetate and ferrous sulfate.

The invention relates to a soo guy chicken processing technique, belonging to the technical field of deep chicken processing. L-cysteine hydrochloride, D-xylopyranose, thiamin hydrochloride and redistilled water are mixed to prepare an aroma enhancing liquid; 3 g of fructus amomi, 18 g of galanga, 18 g of radix angelicae, 1 g of clove, 6g of tsaoko amomum fruit, 18g of cinnamon, 6 g of dried tangerine peel and 3 g of cardamom are wrapped by gauze and then mixed with 250 g of edible salt, 10 g of sodium glutamate to prepare a curing liquid. The processing technique comprises the steps of thawing, cleaning, leaching, curing, shaping, three-sectional drying (drying I, drying II and drying III), vacuum packaging, sterilization, cooling, checking and product finishing. The processing technique can effectively improve the color and the taste of a chicken product, obviously reduce the generation of cancerogenic substances, i.e. naphtho-(alpha)pyrene, and improve the safety, sanitation and eating quality of the chicken product, and is beneficial to improving the economic benefit of relative enterprises and quickening the development of poulard raising industry in China.

The invention discloses a method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue, belongs to the field of breeding by the biological technique, and aims at reducing the alcohol disinfecting time and reducing the alcohol concentration as well as creating foundation for the survival rate of hybridization seedlings. The method is that the MS of an embryo culture is improved; the concentration of KNO3, NH4NO3, KH2PO4 and MnSO4.4H2O is reduced; meanwhile, CuSO4.5H2O, CoCl2.6H2O, niacin, pyridoxine hydrochloride and thiamine hydrochloride are not added; the hardening-seedling technology is innovated; when in hardening seedling of the plants, the hardening-seedling treatment with convenient operation and good effect is carried out, so that the survival rate of the seedlings can be greatly increased. According to the operation of the method, the operation of embryo rescue is simplified; the whole culture costs 30 days only, so that the culture cycle can be greatly reduced; meanwhile, the seedling rate can be greatly increased and up to 94%; therefore, the breeding efficiency of the wild rice and cultivated rice distant hybridization embryo rescue can be increased.

The invention discloses a goose liver essence and a preparation method of the goose liver essence. The goose liver essence comprises the following components by weight ratio: 40-60 parts of goose liver enzyme hydrolysis solution, 1-10 parts of reducing sugar, 1-15 parts of amino acid, 0.1-1.5 parts of thiamine hydrochloride, 0.1-5 parts of disodium 5'-ribonucleotide, 0.1-5 parts of spice powder and 30-45 parts of hydrolyzed vegetable protein solution. The goose liver essence is prepared from the goose liver enzyme hydrolysis solution as well as the reducing sugar, the amino acid, the thiamine hydrochloride, the disodium 5'-ribonucleotide, the spice powder and the hydrolyzed vegetable protein solution through Maillard reaction, wherein the goose liver enzyme hydrolysis solution serves as the main raw material, and the reducing sugar, the amino acid, the thiamine hydrochloride, the disodium 5'-ribonucleotide, the spice powder and the hydrolyzed vegetable protein solution serve as the auxiliary raw materials. Therefore, the prepared product has strong real goose liver characteristic aroma, mellow taste and high nutritional value and can be widely used as the essence of instant noodle seasonings, food ingredients and meat products.

The invention belongs to the technical field of biological engineering, and relates to a culture medium for the spore germination and seedling culturing of pteridophyte, which is characterized by consisting of the following nutritional components: 2 to 60 mmol.L<-1> nitrate ions, 1 to 30 mmol.L<-1> ammonium ions, 0.3 to 8 mmol.L<-1> phosphate radical ions, 2.7 to 66 mmol.L<-1> potassium ions, 0.37 to 12.0 mmol.L<-1> calcium ions, 0.18 to 1.8 mmol.L<-1> magnesium ions, 0.173 to 1.73 mmol.L<-1> sulfate ions, 10 to 100 mumol.L<-1> chelated ferric salt or chelated ferrous salt, 10 to 200 mumol.L<-1> boric acid, 10 to 100 mumol.L<-1> divalent manganese ions, 3 to 30 mumol.L<-1> zinc ions, 0.5 to 8 mumol.L<-1> divalent copper ions or complexes thereof, 0.1 to 1 mumol.L<-1> molybdate ions, 0.01 to 0.1 mumol.L<-1> divalent cobalt ions, 0.05 to 12 mumol.L<-1> chloride ions, 0.3 to 30 mumol.L<-1> thiamine hydrochloride and the balance of water, wherein the pH value of the culture medium is between 4.0 and 9.0. The culture medium can improve the germination rate of spores.

The invention discloses a Chinese hamster ovary culture medium as well as a preparation method and application thereof. The culture medium comprises ascorbic acid, pyridoxine hydrochloride, vitamin B12, nicotinamide, riboflavin, thiamine chloride, choline chloride, folic acid, calcium pantothenate, sodium pyruvate, reduced glutathione, inositol, biotin, hypoxanthine, putrescine, lipoic acid, linoleic acid, thymine, glucose, HEPES (2-hydroxyethyl) buffer solution, dextran sulphate, Pluronic-F68, various amino acids and salts thereof and various inorganic salts. The culture medium has low cost, and the component does not contain animal origin ingredients, does not contain protein and has definite chemical ingredients. All ingredients are cooperated and blended, and the Chinese hamster ovary culture medium can satisfy the basic growth requirement of cells, and can effectively regulate, transfer and control the culture of cells so as to realize good high-density cell culture. Compared with the culture effect of the existing culture medium, the culture effect of culture medium disclosed in the invention is at least equivalent to even superior to the existing culture medium.

The invention discloses a medium for the seed germination of orchids; the medium consists of NH4NO3, KNO3, CaCl2.2H2O, MgSO4.7H2O, KH2PO4, KI, H3BO3, MnSO4.4H2O, ZnSO4.7H2O, Na2MoO4.2H2O, CuSO4.5H2O, CoCl2.6H2O, FeSO4.7H2O, Na2.EDTA, inosite, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, glycine, peptone from casein, mashed potato and sucrose and the volume is limited by water; by using the medium of the invention to culture orchid, not only the seed germination time can be shortened but also the seed germination rate can be improved; the experiment indicates that by using the medium of the invention to culture orchid, only two weeks are needed from seeding to germination and the germination rate is more than 70 percent. The method of the invention is suitable for culturing various caulicolous orchids and has the advantages of simple operation and low cost; therefore, the medium and the method for culturing the orchids of the invention have important promotion value and are applicable to practice use.

The invention provides a culture medium for bletilla striata tissue culture. The culture medium consists of improved MS culture medium: 1890mg / L to 1900mg / L of potassium nitrate, 1650mg / L to 1660mg / L of ammonium nitrate, 440mg / L to 445mg / L of CaCl2.2H2O, 370mg / L to 375mg / L of magnesium sulfate heptahydrate, 165mg / L to 170mg / L of monopotassium phosphate, 0.75mg / L to 0.83mg / L of KI, 0.025mg / L to 0.03mg / L of cobalt chloride, 27.8mg / L to 30mg / L of ferrous sulfate, 24.3mg / L to 26mg / L of ethylenediaminetetraacetic acid, 95mg / L to 100mg / L of inositol, 0.8mg / L to 1mg / L of niacin, 0.8mg / L to 1mg / L of pyridoxine hydrochloride, 0.8mg / L to 1mg / L of thiamine hydrochloride and 3mg / L to 4mg / L of glycine. Bletilla striata grown by the culture medium has the characteristics of strong roots, big corms, less variation phenomenon, high transplanting survival rate and fast growth.

The present invention provides a topical application of thiamin to human skin or mammalian coats to provide effective insect repellency. Any commercially available thiamin compounds such as thiamin hydrochloride or thiamin mononitrate can be combined with a pharmaceutically or cosmetically acceptable carrier for preparing a composition for topical application. This composition and method of application can further be combined with sun-block or sunscreen agents to provide protection against insects as well as the harmful effects of UV radiation. This composition may also contain botanical oils or extracts to mask the thiamin odor and provide repellant synergy.

The invention discloses a basic medium ZS for plant tissue culture and relates to the field of plant propagation. Every 1000 mL of the basic medium ZS contains 3075 mg of potassium nitrate, 578 mg ofammonium phosphate, 491 mg of calcium nitrate, 160 mg of ammonium chloride, 222 mg of magnesium nitrate, 396 mg of ammonium sulfate, 0.83 mg of potassium iodide, 6.2 mg of boric acid, 22.3 mg of manganese sulfate, 8.6 mg of zinc sulfate, 0.25 mg of sodium molybdate, 0.025 mg of copper sulfate, 0.025 mg of cobalt chloride, 100 mg of inositol, 0.1 mg of thiamine hydrochloride (VB1), 0.5 mg of pyridoxine hydrochloride (VB6), 0.5 mg of nicotinic acid, 37.3 mg of ethylenediaminetetraacetic acid disodium salt and 27.8 mg of ferrous sulfate. By means of the basic medium, the same tissue culture effect as an MS medium can be obtained without ammonium nitrate.

The invention relates to a preparation method of a dendritic copper sulphide microcrystal. According to the preparation method, cupric nitrate and thiamine hydrochloride serving as raw materials, deionized water serving as a solvent, and a preparing solution are subjected to hydro-thermal synthesis in a reaction kettle, and then are centrifugally separated, washed, extracted and filtered, and dried in vacuum to obtain a dendritic copper sulphide microcrystal product. The preparation method adopts the thiamine hydrochloride as a sulfur source, thus the dendritic copper sulphide microcrystal is green and nontoxic; and due to that the preparation process is advanced and reasonable, the data are full and accurate, the product has high purity up to 98%, the yield is high and up to 90%, no pollution is brought to the environment, the method is an ideal method for preparing the dendritic copper sulphide microcrystal.

The invention discloses thermally reacted chicken powder and a production process thereof. The thermally reacted chicken powder comprises the following raw materials by weight, wherein the used raw materials comprise two parts: the first part is 140 to 160 grams of water, 17 to 18 grams of sodium hydroxide, 50 to 60 grams of L-cysteine hydrochloride, 16 to 19 grams of thiamine chloride, 0.7 to 0.9 gram of xylose, 30 to 35 grams of glucose, 90 to 100 grams of monosodium glutamate, 20 to 30 grams of gelatin, 0.5 to 1 gram of lecithin and 70 to 80 grams of pure chicken meal; and the second part is 40 to 60 grams of I+G, 600 to 800 grams of monosodium glutamate, 80 to 90 grams of pure chicken meal, 180 to 200 grams of salt, 150 to 200 grams of starch, 90 to 100 grams of flour and 80 to 100 grams of chicken grease. The raw materials are heated, then dried under vacuum and smashed into the powder. The production process is simple; and the produced chicken powder has intense chicken flavor.

The invention discloses a planting method of plateau mine herbaceous plants, comprising the following steps of: preparing soil, seeding, and spraying a nutrient solution, wherein the nutrient solution comprises the following components in proportion: 0.5L of original mixing liquid, 0.5L of water and 100g of water-retaining agent and the original mixing liquid comprises the following components: 1650mg / L of NH4NO3, 1900mg / L of KNO3, 370mg / L of MgSO4 7H2O, 170mg / L of KH2PO4, 440mg / L of CaCl2 2H2O, 0.83mg / L of KI, 6.20mg / L of H3BO3, 22.30mg / L of MnSO4 4H2O, 8.60mg / L of ZnSO4 7H2O, 0.25mg / L of Na2MoO4 2H2O, 0.025mg / L of CuSO4 5H2O, 0.025mg / L of CoCl2 6H2O, 28.70mg / L of FeSO4 7H2O, 37.3mg / L of Na2-EDTA 2H2O, 100 mg / L of inositol, 0.5mg / L of niacin, 0.5mg / L of pyridoxine hydrochloride, 0.1mg / L of thiamine chloride, 2mg / L of glycine and 25g of sucrose; covering soil; and covering mulching film. Through the implementation and the usage of the method, the germination rate and the survival rate and the per-plant tiller number of elymus dahuricus turcz can be greatly increased, therefore, the possibility of recovering vegetation of mines is increased.

The invention discloses an orchid plant growth regulator. The orchid plant growth regulator comprises a solid growth regulator and a liquid growth regulator, wherein the solid growth regulator is prepared from sodium para-nitrophenolate, sodium 2-nitrophenolate, 5-nitroguaiacol sodium salt, thiamine hydrochloride, pyridoxine hydrochloride and cyclohexanhexol in a mixing manner. The orchid plant growth regulator has the high affinity and interactivity to cytoplasm of orchid plants, to be facilitated to enter a sensitive part of a plant body, promotes the photosynthesis and the metabolism, accelerates the synthesis and the accumulation of dry substances, achieves the anti-microbial and anti-virus effects, has the characteristics of environment protection and high efficiency, greatly saves the using amount while the high efficiency is ensured, increases the effective utilization rate of raw materials, reduces the toxicity, facilitates the implementing of the medicinal value of the orchid plants, and has low cost; the orchid plant grows vigorously when the orchid plant growth regulator is used, has a high ornamental value, and has the high quality when being used as medicinal materials, has no toxic and side effects and a good medicinal effect.

The invention discloses a culture medium for delaying the growth of potato tissue cultured seedlings. Every 1L of the culture medium comprises the following components: 627mg-693mg of ammonium nitrate (NH4NO3), 722mg-798mg of potassium nitrate (KNO3), 168mg-184mg of calcium chloride (CaCl2.2H2O), 160mg-176mg of magnesium sulfate (MgSO4.7H2O), 65mg-71mg of monopotassium phosphate (KH2PO4), 0.315mg-0.348mg of potassium iodide (KI), 2.36mg-2.60mg of boric acid (H3BO3), 6.43mg-7.09mg of manganese sulfate (MnSO4.4H2O), 3.27mg-3.61mg of zinc sulfate (ZnSO4.7H2O), 0.095mg-0.105mg of sodium molybdate (Na2MoO4.2H2O), 0.009mg-0.011mg of copper sulfate (CuSO4.5H2O), 0.009mg-0.011mg of cobalt chloride (CoCl2.6H2O), 28.5mg-31.5mg of ferric sodium edelate (FeNa2EDTA.H2O), 38mg-42mg of inositol (C6H12O6), 0.19mg-0.21mg of nicotinic acid (C6H5NO2), 0.19mg-0.21mg of pyridoxine hydrochloride (C8H11NO3.HCl), 0.038mg-0.042mg of thiamine hydrochloride (C12H17ClN4OS.HCl), 7600mg-8400mg of cane sugar (C12H22O11), 7600mg-8400mg of mannitol, 6650mg-7350mg of agar and the balance of water. By adopting the culture medium disclosed by the invention, the potato tissue cultured seedlings grow slowly and need 140-150 days to grow to a height of 8cm since the inoculation date, thus the preservation time of the potato seedlings is remarkably prolonged.

The invention relates to a supplementing culture medium for CHO cell culture and a CHO cell culture method. The supplementing culture medium comprises amino acids, vitamins, sodium hydroxide and waterfor injection. According to the total mass of the supplementing culture medium, the supplementing culture medium comprises, by mass, 4.8-19.75% of amino acids, 0.00925-0.13% of vitamins, 1.25-5.5% ofsodium hydroxide and the balance water for injection, wherein the amino acids include L-aspartic acid, L-tyrosine, L-threonine, L-tryptophan and L-glutamic acid, and the vitamins include riboflavin,folic acid, vitamin B12, biotin, menadione sodium bisulfite, thiamine hydrochloride and L-ascorbic acid. By means of the supplementing culture medium and the cell culture method, CHO cell growth can be optimized, and the capacity of cells of expressing target protein can be improved.

The invention belongs to a dendrobium candidum sprouting and rooting culture medium, and a preparation method and a cultivation method thereof. The culture medium is characterized by comprising potassium nitrate, ammonium nitrate, monopotassium phosphate, magnesium sulphate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, cupric sulfate, cobaltous chloride, disodium EDTA, ferrous sulfate, phaseomannite, glycine, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, cane sugar, agar and distilled water by weight. The invention solves the technical problem of extremely low natural fertility, as well as increase of contamination rate and high cost of the culture medium, which are caused by twice manual transplanting and cultivating. The dendrobium candidum of the sprouting and rooting two-in-one culture medium can meet the demands of both production and people.

The invention discloses a whey protein solid beverage. The whey protein solid beverage is prepared from the following raw materials: concentrated whey protein powder, inulin, resistant dextrin, a flavoring agent, vitamin A acetate, vitamin C, vitamin E acetate, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, vitamin B12, folic acid, calcium pantothenate, ferrous fumarate, zinc sulfate, maltodextrin, natural strawberry essence, silicon dioxide and stevioside. According to the whey protein solid beverage disclosed by the invention, the raw materials have high protein content and contain various types of vitamins, so that the absorption of proteins can be accelerated, the water solubility is relatively high and the mouthfeel is more fine and smooth. The whey protein solid beverage disclosed by the invention has the effects of supplementing physical ability, nutrients and various types of the vitamins, protecting eyesight and the like. The whey protein solid beverage has simple and reasonable raw material composition, natural components and a proper proportion; when being brewed with water, the whey protein solid beverage is more uniformly dispersed and is convenient for oral administration; the whey protein solid beverage is a solid beverage suitable for people.

A sports drink mixture for making a sports drink that remains palatable while providing nutritional and energy benefits without causing negative physiological responses in the human body includes compositions of: tapioca starch, further including compositions of unprocessed and pregelatinized tapioca starch; bromelain; natural flavors; citric acid; vitamin E oil; stevia; potassium phosphate; sodium citrate; magnesium chloride; ascorbic acid; niacinamide; d-calcium pantothenate; pyridoxine hydrochloride; riboflavin; thiamine hydrochloride; folic acid; biotin; and cyanocobalamin. A sports drink includes compositions of the sports drink mixture with an aqueous liquid.

The invention provides a formula of a culture medium for culturing tobacco anther. The formula comprises the following components in part by weight: 2,800 to 32,000 parts of sucrose, 6,000 to 8,000 parts of agar, 680 to 760 parts of NH4NO3, 880 to 920 parts of KNO3, 130 to 190 parts of CaCl2.2H2O, 150 to 210 parts of MgSO4.7H2O, 60 to 80 parts of NaH2PO4, 50 to 70 parts of Na2-ethylene diamine tetraacetic acid (EDTA), 40 to 50 parts of FeSO4.7H2O, 20 to 30 parts of MnSO4.4H2O, 15 to 25 parts of ZnSO4.7H2O, 15 to 25 parts of H3BO4, 0.3 to 0.7 part of thiamine chloride, 0.3 to 0.7 part of niacin, 0.3 to 0.7 part of pyridoxine hydrochloride, 90 to 110 parts of inositol and 1 to 3 parts of glycine. The pH value is 5.5 to 6.0.

The invention discloses a method for forcing dunaliella tertiolecta to accumulate beta-carotene and belongs to the aquaculture field. Conventionally, the dunaliella tertiolecta adopts an open type runway big pool and uses an Hutner culture solution to culture, is slow in growth, low yin yield, easy to pollute, high in cost and low in content of beta-carotene, and the illumination intensity and the temperature are completely dependent on nature. In order to overcome the defects in the prior art, indoor light-control temperature-control culture is adopted, the illumination intensity and the temperature are regulated according to the special needs of different growth stages of the dunaliella tertiolecta, especially at the later culture period, the illumination intensity and the temperature are increased, ammonium chloride, human urine, calcium phosphate, thiamine hydrochloride and the like are supplemented, the conventional bath culture method is changed; after the later culture period, nitrogen salt and phosphor salt in the culture solution are removed, the salinity is improved, the growth speed of the dunaliella tertiolecta is greatly increased, and the content of the beta-carotene is increased by 70%.

The invention discloses a solid culture medium for culturing garlic epigamous embryo, belonging to the field of agricultural biotechnology. The solid culture medium includes the following components: potassium nitrate, ammonium nitrate, monopotassium phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulphate, cobalt chloride, ferric sulfate, sodium ethylene diamine tetracetate, cyclohexanehexol, nicotinic acid, puridoxine hydrochloride, thiamine hydrochloride, glycin, coconut cream, sucrose, 6-benzylamino adenine or zeatin, indoleacetic acid or rhodofix and agaragar. The solid culture medium for culturing garlic epigamous embryo has the advantages of good culturing effect and high success rate; based on the original foundation, the planting percent is increased by more than 30% and the propagation coefficient is improved by two times, thus greatly improving the culturing success rate of the garlic epigamous embryo or the whole plant thereof and solving the key technology of the difficult problem that the garlic can not be reproduced sexually.

The present invention belongs to the technical field of soil environments, and specifically relates to a microbial soil improvement agent for petroleum polluted soil. The microbial soil improvement agent comprises the following raw materials: Paxillus involutus Hypha, Hebeloma mesophaeusm, Acinetobacter calcoaceticus, Sphingomonas paucimobilis, pyrene degrading bacteria, Glomus etunicatum, Cephalosporium roseum, Aureobasidium pullulans, nitrilase, long-chain alkane degradation enzyme, integration membrane di iron alkane hydroxylase, phenylalanine deaminase, arginine dihydrolase, Rhodococcus erythropolis, pseudomonas aeruginosa, sodium oleate, thiamine hydrochloride, calcium pantothenate, oyster shell powder, vermiculite powder, paspalum wettsteinii hackel powder, kentucky eupatorium fortunei powder, miscanthus floridulus powder, medicago sativa powder, pig manure granules, goat manure granules, gamma-polyglutamic acid, malic acid, citric acid, and Nostocales powder. According to the present invention, various types of the microorganisms in the improvement agent synergistically coordinate, and co-act with the carrier raw materials; and with the application of the improvement agent to treat different types of crude oil polluted soil in the Shengli oil field, the total removal rate of the petroleum hydrocarbon in the polluted soil achieves 77.68-89.76%.

An Oryza meyeriana in vitro immature embryo medium contains KNO2, (NH4)3SO4, KH2PO4, MgSO4.7H2O, CaCl2.2H2O, MnSO4.7H2O, ZnSO4.7H2O, H3BO4, KI, NaMoO4.2H2O, CuSO4.5H2O, CoCl.6H2O, glycine, thiamine hydrochloride, pyridoxol hydrochloride, nicotinic acid, inositol, lactoalbumin hydrolysate, a ferric salt, sucrose, 6-BA, IAA and agar. Above components include various nutritional components and contents needed in the Oryza meyeriana in vitro immature embryo culture process, so the medium has the advantages of high emergence rate and high survival rate of immature embryos.

The invention belongs to the field of bark regenerants, and particularly discloses a bark regenerant. The bark regenerant at least comprises one of a bark regenerant I, a bark regenerant II and a bark regenerant III, wherein the bark regenerant I is prepared from nicotinic acid, glycine, thiamine hydrochloride, pyridoxine hydrochloride, vitamin, naphthylacetic acid and indolebutyric acid; the bark regenerant II is prepared from vitamin C, amoxicillin sulbactam, a povidone iodine preparation, polyvinylpyrrolidone, naphthylacetic acid and indolebutyric acid; the bark regenerant III is prepared from inositol, nicotinic acid, glycine, thiamine hydrochloride, pyridoxine hydrochloride, vitamin C, potassium iodide, sodium sulfate, potassium nitrate, calcium nitrate tetrahydrate, magnesium sulfate heptahydrate, manganese sulfate tetrahydrate, zinc sulphate heptahydrate, sodium molybdate, copper sulfate pentahydrate, ferric sulfate, naphthylacetic acid and indolebutyric acid. The bark regenerant can effectively promote regeneration of new barks of peeled trees, is general and is extremely good in use effect in most tree species.

The invention discloses a synthetic medium for Staphylococcus carnosus, and a preparation method and application of Staphylococcus carnosus fermentation broth. The synthetic medium for Staphylococcus carnosus comprises the following raw materials by weight: on the basis of a volume of 1 L, 10 to 50 g of anhydrous glucose, 0.1 to 1.5 g of lysine, 1.0 to 5.0 g of valine, 0.1 to 1.5 g of glycine, 1.0 to 5.0 g of arginine, 1.0 to 5.0 g of proline, 5 to 10.0 g of glutamic acid, 0.1 to 0.5 g of tryptophan, 0.1 to 1.5 g if cystine, 1.0 to 3.0 mg of thiamine hydrochloride, 1.0 to 3.0 mg of calcium pantothenate, 1.0 to 3.0 mg of nicotinic acid, 1.0 to 3.0 mg of manganese sulfate, 0.1 to 1.0 g of potassium dihydrogen phosphate, 0.1 to 0.8 g of magnesium sulfate heptahydrate, 0.005 to 0.010 g of ferrous sulfate heptahydrate, 1.0 to 5.0 g of dipotassium hydrogen phosphate trihydrate and 6 to 10 g of 3-(N-morpholino)propanesulfonic acid, with the balance being water. The invention also discloses a preparation method for the Staphylococcus aureus fermentation broth. The synthetic medium for Staphylococcus carnosus is reasonable in composition, rich in nutrients and capable of meeting the growth demands of Staphylococcus carnosus. The growth amount of Staphylococcus carnosus obtained through the synthetic medium provided by the invention is increased by 5% compared with the growth amount of Staphylococcus carnosus obtained through frequently used LB (Luria-Bertani) mediums.