The invention discloses a culture medium for promoting
germination of
saussurea involucrata seeds and increasing the flavone content in a
cell culture and application of the culture medium. Multivitamins for promoting
germination of
saussurea involucrata seeds and increasing the flavone content in the
cell culture comprise
thiamine hydrochloride VB1, nicotinic acid and
pyridoxine hydrochloride VB6; wherein the concentration of
thiamine hydrochloride VB1 is 1000 mg / L, the concentration of nicotinic acid is 500 mg / L, and the concentration of
pyridoxine hydrochloride VB6 is 500 mg / L. A mixed mother solution (V3
vitamin for short) of three vitamins is prepared for
filtration, sterilization and addition, the concentrations of the three vitamins are
thiamine hydrochloride VB11000 mg / L, nicotinic acid 500 mg / L and
pyridoxine hydrochloride VB6500 mg / L respectively, the overall addition amount of V3 is 1 mL / L, the
germination rate of
saussurea involucrata seeds can be effectively improved, and the germination rate of the seeds is 40.58%.