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Formula of culture medium for culturing tobacco anther
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A culture medium formula, technology of tobacco flowers, applied in the field of medium for plant tissue culture, can solve problems such as complicated research, increased rate of deformed seedlings, and non-solidified medium
Inactive Publication Date: 2011-08-10
GUIZHOU UNIV +1
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Problems solved by technology
Activated carbon has the effect of generally increasing the emergence rate in anther culture (Bu Xuexian [1998] the adsorption of activated carbon to growth-regulating substances in the medium; Liu Yongsheng [1994] the use of activated carbon in plant tissue culture; Wang Jingju [1983] improved sugarcane tissue Research on culture efficiency; Zhu Zhiqing [1988] Anther and anther culture. Economic plant tissue culture; Jia Xinghua [1991] Tobacco flower cultivation; Cao Ziyi [1996] Practical plant tissue culture technology tutorial; Anagnostakis SL [1974] Haploid plants from anthers of tobacco enhancement with charcoal; Johansson L[1982]Improrement of anther culture technique; Fridborg G[1975] Effects of activated charcoal on growth and morphogenesis in cell culture Physiol Plant; Smith RH[1983]In vitro propagation of Nadina domestica. Hortscience, etc.), However, activated carbon has side effects such as preventing the medium from solidifying, increasing the rate of deformed seedlings, and complicating the research (Bu Xuexian [1998] the adsorption of activated carbon to growth-regulating substances in the medium; Liu Yongsheng [1994] the use of activated carbon in plant tissue culture ; Jia Xinghua [1991] Tobacco flower cultivars); especially the increased rate of deformed seedlings, resulting in a decrease in the number of normally available seedlings (Jia Xinghua [1991] Tobacco flower cultivars), sometimes even less than the treatment without activated carbon, for obtaining haploid It is very unfavorable to study for the purpose of somatic plants
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Embodiment 1
[0062] Embodiment 1: The medium formula for cultivating tobacco anthers, calculated in parts by weight, it includes 30000 parts of sucrose, 7000 parts of agar, NH 4 NO 3 720 copies, KNO 3 900 parts, CaCl 2 2H 2 O160 parts, MgSO4 7H2O180 parts, NaH 2 PO 4 68 parts, Na 2 -60 parts of EDTA, FeSO 4 ·7H 2 O45 parts, MnSO 4 4H 2 O25 parts, ZnSO 4 ·7H 2 O20 parts, H 3 BO 4 20 parts, 0.5 parts of thiaminehydrochloride, 0.5 parts of niacin, 0.5 parts of pyridoxinehydrochloride, 100 parts of inositol and 2 parts of glycine; the pH value is 5.8.
Embodiment 2
[0063] Embodiment 2: The medium formula for cultivating tobacco anthers, calculated in parts by weight, it includes 28000 parts of sucrose, 8000 parts of agar, NH 4 NO 3 680 copies, KNO 3 880 parts, CaCl 2 2H 2 O190 parts, MgSO4 7H2O150 parts, NaH 2 PO 4 80 parts, Na 2 -50 parts of EDTA, FeSO 4 ·7H 2 O50 parts, MnSO 4 4H 2 O20 parts, ZnSO 4 ·7H 2 O25 parts, H 3 BO 4 15 parts, 0.3 parts of thiamine hydrochloride, 0.7 parts of niacin, 0.3 parts of pyridoxine hydrochloride, 90 parts of inositol and 3 parts of glycine; the pH value is 6.0.
Embodiment 3
[0064] Embodiment 3: The culture medium formula for cultivating tobacco anthers, calculated in parts by weight, it includes 32000 parts of sucrose, 6000 parts of agar, NH 4 NO 3 760 copies, KNO 3 920 parts, CaCl 2 2H 2 O130 parts, MgSO4·7H2O210 parts, NaH 2 PO 4 60 parts, Na 2 -70 parts of EDTA, FeSO 4 ·7H 2 O40 parts, MnSO 4 4H 2 O30 parts, ZnSO 4 ·7H 2 O15 parts, H 3 BO 4 25 parts, 0.7 parts of thiamine hydrochloride, 0.3 parts of niacin, 0.7 parts of pyridoxine hydrochloride, 110 parts of inositol and 1 part of glycine; the pH value is 5.5.
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Abstract
The invention provides a formula of a culture medium for culturing tobacco anther. The formula comprises the following components in part by weight: 2,800 to 32,000 parts of sucrose, 6,000 to 8,000 parts of agar, 680 to 760 parts of NH4NO3, 880 to 920 parts of KNO3, 130 to 190 parts of CaCl2.2H2O, 150 to 210 parts of MgSO4.7H2O, 60 to 80 parts of NaH2PO4, 50 to 70 parts of Na2-ethylenediamine tetraacetic acid (EDTA), 40 to 50 parts of FeSO4.7H2O, 20 to 30 parts of MnSO4.4H2O, 15 to 25 parts of ZnSO4.7H2O, 15 to 25 parts of H3BO4, 0.3 to 0.7 part of thiaminechloride, 0.3 to 0.7 part of niacin, 0.3 to 0.7 part of pyridoxinehydrochloride, 90 to 110 parts of inositol and 1 to 3 parts of glycine. The pH value is 5.5 to 6.0.
Description
technical field [0001] The invention relates to a culture medium for planttissue culture, in particular to a medium formula for cultivating tobacco anthers. Background technique [0002] Haploid plants are important materials for genetic research and haploid breeding. At present, anther culture is the most effective method to produce haploid plants. Activated carbon has the effect of generally increasing the emergence rate in anther culture (Bu Xuexian [1998] the adsorption of activated carbon to growth-regulating substances in the medium; Liu Yongsheng [1994] the use of activated carbon in planttissue culture; Wang Jingju [1983] improved sugarcane tissue Research on culture efficiency; Zhu Zhiqing [1988] Anther and anther culture. Economic planttissue culture; Jia Xinghua [1991] Tobacco flower cultivation; Cao Ziyi [1996] Practical plant tissue culture technology tutorial; Anagnostakis SL [1974] Haploid plants from anthers of tobacco enhancement with charcoal; Johansson...
Claims
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